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The map label for this gene is aspS [H]

Identifier: 91781802

GI number: 91781802

Start: 464088

End: 465887

Strand: Direct

Name: aspS [H]

Synonym: Bxe_A4043

Alternate gene names: 91781802

Gene position: 464088-465887 (Clockwise)

Preceding gene: 91781801

Following gene: 91781803

Centisome position: 9.48

GC content: 62.78

Gene sequence:

>1800_bases
ATGTCGATGAGATCTGAATACTGCGGTCTGGTGACCGAAGAACTGCTGGGCCAATCCGTCTCGCTGTGCGGCTGGGTGAG
CCGCCGCCGCGACCACGGCGGCGTCATCTTCATCGACCTGCGCGATCGCGAAGGCCTCGTTCAGGTCGTCTGCGACCCGG
ACCGCGCGGAGATGTTCAAGGCGGCCGAAGGCGTGCGCAACGAGTTCTGCCTGCAGATCAAGGGCGTGGTTCGCGCCCGT
CCGGAAGGCACCACGAACGCTGCGCTGAAGAGCGGCAAGATCGAAGTGCTGTGCCACGAGCTGATCGTGCTGAACCCGTC
GATCACGCCGCCGTTCCAGCTCGACGACGACAACCTGTCGGAAACCACGCGCCTCACGCACCGCGTGCTGGACCTGCGCC
GTCCGCAGATGCAGCACAACCTGCGTCTGCGTTACCGCGTCGCGATCGAAGTGCGCAAGTACCTGGACGCGCAAGGCTTC
ATCGACATCGAAACGCCGATGCTCACCAAGAGCACGCCGGAAGGCGCGCGCGACTACCTGGTGCCGTCGCGTACGAACCC
GGGCCAGTTCTTCGCGCTGCCGCAATCGCCGCAGCTGTTCAAGCAGTTGCTGATGGTGGCGAACTTCGATCGCTACTACC
AGATCGTCAAGTGCTTCCGCGACGAAGATCTGCGCGCCGACCGTCAGCCGGAATTCACGCAGATCGACTGCGAAACCTCG
TTCCTGTCGGAACAGGAAATCCGCGATCTGTTCGAAGCGATGATCCGTCACGTGTTCAAGGAAACGATCGGCGTCACGCT
GGACGAGAAATTCCCGGTCATGCTGTATTCGGAAGCCATGCGCCGCTTCGGTTCGGACAAGCCGGACCTGCGCGTGAAGC
TCGAATTCACGGACCTCACGGACGCCGTTCGCGACGTCGACTTCAAGGTGTTCAGCACGCCGGCCAACACGAAAGACGGC
CGTGTCGCGGCGATTCGCGTGCCGAAGGGCGGCGAGCTCTCGCGTGGCGACATCGACAGCTACACGGAATTCGTGCGCAT
CTACGGTGCGAAGGGTCTGGCGTGGATCAAGGTCAACGAAGTCGCGAAGGGCCGTGACGGTCTGCAAAGCCCGATCGTCA
AGAACCTGCACGACGAAGCGGTGAAGGCGATCATCGAGCGCACCGGCGCGCAGGACGGCGACATCATTTTCTTCGCGGCG
GATCGCGCGAAAGTGGTGAACGACAGCCTCGGCGCGCTGCGTCTGAAGATCGGTCATTCGGAATTCGGCAAGGCCAATGG
TCTGGTGGAAAGCGGCTGGAAGCCGCTGTGGGTGATCGACTTCCCCATGTTCGAATACGACGAGGAAGACAACCGCTACG
TCGCCGCGCATCACCCGTTCACGAGCCCGAAAGACGAGCACCTCGAGTATCTGGAAACGGATCCGGCGCGCTGCCTCGCG
AAGGCCTACGACATGGTGCTGAACGGCTGGGAAATCGGCGGCGGTTCGGTGCGTATCCACCGTGAAGAAGTGCAGAGCAA
GGTGTTCCGCGCGCTGAAGATCAACGCGGAAGAAGCGCAGGCCAAGTTCGGCTTCCTGCTCGACGCGCTGCAATACGGCG
CGCCGCCGCACGGCGGTATCGCATTTGGTCTCGACCGCATCGTCACGATGATGGCCGGCGCCGATTCGATCCGCGACGTG
ATCGCGTTCCCGAAAACGCAGCGCGCGCAGTGTCTGCTGACGCAGGCGCCGAGCGAAGTGGACGAGCGCCAGTTGCGCGA
GTTGCACATTCGTCTGCGCCAGCCGGAACCGAAGGCGTAA

Upstream 100 bases:

>100_bases
GTGCGCCGCACGACAGTCGAGCCTCCGCTGTAATGCCGGCGCGCTGCTGTGTTTCACTGCGGCGCGCCGTTCAACCAATG
CAAAACGAAAGACAAACATC

Downstream 100 bases:

>100_bases
TGGCATAAGCGGAACGAGTGCTGTCTGAAAACGACAGCGGGCAGCAACCGGAAAAGGCGCGTCATGCGCCTTTTTCGTTT
TTTGCGTTACAGTCGATGCA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MSMRSEYCGLVTEELLGQSVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFKAAEGVRNEFCLQIKGVVRAR
PEGTTNAALKSGKIEVLCHELIVLNPSITPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEVRKYLDAQGF
IDIETPMLTKSTPEGARDYLVPSRTNPGQFFALPQSPQLFKQLLMVANFDRYYQIVKCFRDEDLRADRQPEFTQIDCETS
FLSEQEIRDLFEAMIRHVFKETIGVTLDEKFPVMLYSEAMRRFGSDKPDLRVKLEFTDLTDAVRDVDFKVFSTPANTKDG
RVAAIRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDEAVKAIIERTGAQDGDIIFFAA
DRAKVVNDSLGALRLKIGHSEFGKANGLVESGWKPLWVIDFPMFEYDEEDNRYVAAHHPFTSPKDEHLEYLETDPARCLA
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKINAEEAQAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQCLLTQAPSEVDERQLRELHIRLRQPEPKA

Sequences:

>Translated_599_residues
MSMRSEYCGLVTEELLGQSVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFKAAEGVRNEFCLQIKGVVRAR
PEGTTNAALKSGKIEVLCHELIVLNPSITPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEVRKYLDAQGF
IDIETPMLTKSTPEGARDYLVPSRTNPGQFFALPQSPQLFKQLLMVANFDRYYQIVKCFRDEDLRADRQPEFTQIDCETS
FLSEQEIRDLFEAMIRHVFKETIGVTLDEKFPVMLYSEAMRRFGSDKPDLRVKLEFTDLTDAVRDVDFKVFSTPANTKDG
RVAAIRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDEAVKAIIERTGAQDGDIIFFAA
DRAKVVNDSLGALRLKIGHSEFGKANGLVESGWKPLWVIDFPMFEYDEEDNRYVAAHHPFTSPKDEHLEYLETDPARCLA
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKINAEEAQAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDV
IAFPKTQRAQCLLTQAPSEVDERQLRELHIRLRQPEPKA
>Mature_598_residues
SMRSEYCGLVTEELLGQSVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFKAAEGVRNEFCLQIKGVVRARP
EGTTNAALKSGKIEVLCHELIVLNPSITPPFQLDDDNLSETTRLTHRVLDLRRPQMQHNLRLRYRVAIEVRKYLDAQGFI
DIETPMLTKSTPEGARDYLVPSRTNPGQFFALPQSPQLFKQLLMVANFDRYYQIVKCFRDEDLRADRQPEFTQIDCETSF
LSEQEIRDLFEAMIRHVFKETIGVTLDEKFPVMLYSEAMRRFGSDKPDLRVKLEFTDLTDAVRDVDFKVFSTPANTKDGR
VAAIRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKVNEVAKGRDGLQSPIVKNLHDEAVKAIIERTGAQDGDIIFFAAD
RAKVVNDSLGALRLKIGHSEFGKANGLVESGWKPLWVIDFPMFEYDEEDNRYVAAHHPFTSPKDEHLEYLETDPARCLAK
AYDMVLNGWEIGGGSVRIHREEVQSKVFRALKINAEEAQAKFGFLLDALQYGAPPHGGIAFGLDRIVTMMAGADSIRDVI
AFPKTQRAQCLLTQAPSEVDERQLRELHIRLRQPEPKA

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=610, Percent_Identity=38.6885245901639, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI45439306, Length=277, Percent_Identity=28.158844765343, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI194272210, Length=248, Percent_Identity=22.9838709677419, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI5031815, Length=248, Percent_Identity=22.9838709677419, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=58.3756345177665, Blast_Score=709, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=297, Percent_Identity=25.2525252525253, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790571, Length=257, Percent_Identity=25.6809338521401, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI32566633, Length=602, Percent_Identity=33.8870431893688, Blast_Score=317, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI71994340, Length=276, Percent_Identity=22.8260869565217, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535925, Length=276, Percent_Identity=22.8260869565217, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535927, Length=276, Percent_Identity=22.8260869565217, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551876, Length=262, Percent_Identity=25.1908396946565, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=300, Percent_Identity=38, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6323011, Length=281, Percent_Identity=27.7580071174377, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=258, Percent_Identity=25.968992248062, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=275, Percent_Identity=24.3636363636364, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6324256, Length=174, Percent_Identity=29.3103448275862, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24584738, Length=600, Percent_Identity=36, Blast_Score=351, Evalue=8e-97,
Organism=Drosophila melanogaster, GI17136276, Length=264, Percent_Identity=30.3030303030303, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640849, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24640851, Length=256, Percent_Identity=25.78125, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67881; Mature: 67750

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMRSEYCGLVTEELLGQSVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFK
CCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCHHHHHH
AAEGVRNEFCLQIKGVVRARPEGTTNAALKSGKIEVLCHELIVLNPSITPPFQLDDDNLS
HHHHHHHHHEEEEEHHHCCCCCCCCHHHHCCCCCEEEEEEEEEECCCCCCCCEECCCCCH
ETTRLTHRVLDLRRPQMQHNLRLRYRVAIEVRKYLDAQGFIDIETPMLTKSTPEGARDYL
HHHHHHHHHHHHCCCHHHCCCEEEEEEEEHHHHHCCCCCEEEECCCCCCCCCCCCCHHCC
VPSRTNPGQFFALPQSPQLFKQLLMVANFDRYYQIVKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLSEQEIRDLFEAMIRHVFKETIGVTLDEKFPVMLYSEAMRRFGSDKPDLRVKLEFTDLT
HCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECHHH
DAVRDVDFKVFSTPANTKDGRVAAIRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKVNE
HHHHHCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
VAKGRDGLQSPIVKNLHDEAVKAIIERTGAQDGDIIFFAADRAKVVNDSLGALRLKIGHS
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCC
EFGKANGLVESGWKPLWVIDFPMFEYDEEDNRYVAAHHPFTSPKDEHLEYLETDPARCLA
CCCCCCCCHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHH
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKINAEEAQAKFGFLLDALQYGAPPHGGI
HHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCH
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQCLLTQAPSEVDERQLRELHIRLRQPEPKA
HHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SMRSEYCGLVTEELLGQSVSLCGWVSRRRDHGGVIFIDLRDREGLVQVVCDPDRAEMFK
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEECCCCCEEEEEECCCHHHHHH
AAEGVRNEFCLQIKGVVRARPEGTTNAALKSGKIEVLCHELIVLNPSITPPFQLDDDNLS
HHHHHHHHHEEEEEHHHCCCCCCCCHHHHCCCCCEEEEEEEEEECCCCCCCCEECCCCCH
ETTRLTHRVLDLRRPQMQHNLRLRYRVAIEVRKYLDAQGFIDIETPMLTKSTPEGARDYL
HHHHHHHHHHHHCCCHHHCCCEEEEEEEEHHHHHCCCCCEEEECCCCCCCCCCCCCHHCC
VPSRTNPGQFFALPQSPQLFKQLLMVANFDRYYQIVKCFRDEDLRADRQPEFTQIDCETS
CCCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHH
FLSEQEIRDLFEAMIRHVFKETIGVTLDEKFPVMLYSEAMRRFGSDKPDLRVKLEFTDLT
HCCHHHHHHHHHHHHHHHHHHHHCCEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECHHH
DAVRDVDFKVFSTPANTKDGRVAAIRVPKGGELSRGDIDSYTEFVRIYGAKGLAWIKVNE
HHHHHCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
VAKGRDGLQSPIVKNLHDEAVKAIIERTGAQDGDIIFFAADRAKVVNDSLGALRLKIGHS
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHCCCCCEEEEEECCC
EFGKANGLVESGWKPLWVIDFPMFEYDEEDNRYVAAHHPFTSPKDEHLEYLETDPARCLA
CCCCCCCCHHCCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHH
KAYDMVLNGWEIGGGSVRIHREEVQSKVFRALKINAEEAQAKFGFLLDALQYGAPPHGGI
HHHHHHHCCEEECCCEEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCH
AFGLDRIVTMMAGADSIRDVIAFPKTQRAQCLLTQAPSEVDERQLRELHIRLRQPEPKA
HHHHHHHHHHHHCHHHHHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA