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The map label for this gene is kefB [H]

Identifier: 91781730

GI number: 91781730

Start: 379570

End: 381570

Strand: Direct

Name: kefB [H]

Synonym: Bxe_A4116

Alternate gene names: 91781730

Gene position: 379570-381570 (Clockwise)

Preceding gene: 91781718

Following gene: 91781731

Centisome position: 7.75

GC content: 65.22

Gene sequence:

>2001_bases
ATGATTTCCCCGCTCGAAATGACGCTGTTCCTGCTGCTGGCATCGGTAGCGGGCGTGGTGACCTTTCGCTTTCTGAATCT
TCCGCCCATGCTCGGCTATCTGACGGTGGGCATCGTGGTCGGGCCGCATGCGTTCGGTTTGATTCCCGACTCGGCGGGCG
CGCAGAACCTGGCGGAATTCGGCGTCGTGTTCCTGATGTTTTCGATCGGGCTGGAGTTCTCGCTCGCCAAGCTGCGTTCC
ATGCGCCGCCTCGTGTTCGGTCTCGGCCTGCTGCAGGTGCTCGGCACGATCGCGGTGGCGGTCACGCTCGGCTTCGTGCT
GGAACGCTGGGTGCATATCACGTGGCAGGCGAGCGTGGCGCTGGGCGGCGCGCTCGCCATGTCGTCGACGGCGATCGTCA
GCAAGATGCTGGCGGAGCGGTTGGAGATCGAGACGGAGCACGGCCGCAATATCTTCGGCGTGCTGCTGTTCCAGGATCTG
GCGGTGGTGCCGCTGCTCATCGTCATCGCGGCGCTCGGCGGCAGTTCCAAAGATCTCGTGAGCTCGCTCGGCGTGGCGGC
CATCAAGATCGTCGTGGCGCTGTCGCTGCTGCTGATCGTCGGGCAGCGTTTCATGACGCGCTGGTTCAACGTGGTGGCGC
GGCGCCGTTCGCAGGAACTGTTCATTCTGAATCTGTTGCTGGTGACGCTCGGTTGCGCGTTCATCACCGACAAGTTCGGG
TTGTCGCTCGCGCTCGGCGCATTCATTGCCGGCATGCTGATCGCCGAGACGCCTTACCGGCATCAGGTGGAAGAAGATAT
CAAGCCGTTTCGCGACGTGCTGCTCGGGCTCTTCTTCATCACCACCGGCATGCTGCTCAATCCGCGTGTGATCTGGGAGC
ATCCGCTGATCGTGCTCGGCTTCCTGGTCGGGCCGATCCTGCTGAAAGCGGTCATGATCACAGGCCTCTCGCGACTGTTC
GGCGCGTCGCCGGGCGTCGCGATGCGCACCGGCATCGGCCTCGCGCAAGCGGGCGAGTTCGGCTTCGTGCTGCTGAATCT
GATTCTCGACAAGCATCTGGTCGACGCCACCCTGCTGCAGGCGATTCTCGCCGCCATGCTGCTGTCCATGCTGGCCGCGC
CGTTCCTGATCCAGAACGCGGATCGCATCGTGCTGCGCCTGTCGTCGACTGAATGGATGATGCAGTCGTTGCAGATGACG
CGCATCGCCACGCAGAGCCTGAAACAAAGCGGCCACGTGATCATTTGCGGGTACGGCCGGGCCGGACAGAATCTGGCGCG
CATGCTGGAGCACGAAGGGTTGTCGTACGTCGCGCTGGACCTGGATCCCGACCGCGTCGCGGCGGCGGCGGCGGCGGGCG
AGTCGGTCGTGTTCGGCGACGCCGGGCGGCGCGAGTCGCTGCTCGCCGCCGGTATCCACCGCGCGGCCGCGATTGCGATC
ACCTACGCGAACACGCCATCGGCATTGCGCGTGCTGCATAACATTCACGAACTCGAACCCACGCTGCCGGTAATCGTGCG
TACCGTCGACGACGCCGATCTGGAAAAGCTGCTGGCCGCGGGCGCGACCGAAGTGATTCCGGAAATCGTCGAAGGCAGTC
TGATGCTTGCCTCGCACACGCTGGTGGTGATGGGCGTGCCGATGCGGCGGGTGGTGCGGCGGGTCGAGGAAATGCGCGAT
GAGCGCTATGCGCTGCTGCGCGGCTATTTCCACGGCGCCGACGACGTGGACGACGACGACGGCCACGAACAGGTGCGGCT
ACAATCGGTGCCGGTCGACGAAAACTCGGACGCCGTGGGCCGCACGCTCGCGGAACTGGGCCTTTTCGAGGTGGGCGTCG
AAGTGACGGCGATTCGCCGGCACGGCATTCGCGGCGTCGAGCCGGACCCGTCCACCAAGCTGCGTTCGAGCGATATCGTG
GTGCTGCGCGGTTTGCCCGAACAGCTGGCGGAGGCCGAGGAACGGCTCTCGAAGCATCGGCGCGCGGGCGCCGCCGCCTA
G

Upstream 100 bases:

>100_bases
TTTATTGCTGCCTTTAACGGCCCCTCAACCTTGTCGCACGGCGTTTGGCGTGCGCGGTTCCGCTGACGAACGAGGACGCT
TTACCCATGCGTTTTTGCCG

Downstream 100 bases:

>100_bases
CGCCTGTACCCCGGCTGCGCGCGTTCCCGCCGCGGCATCCTGGTTTGGTTTTTCATCGGACCTGTCGAGGTCCATCCGGA
GACATCATGTCCAACGCGCT

Product: putative glutathione-regulated potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 666; Mature: 666

Protein sequence:

>666_residues
MISPLEMTLFLLLASVAGVVTFRFLNLPPMLGYLTVGIVVGPHAFGLIPDSAGAQNLAEFGVVFLMFSIGLEFSLAKLRS
MRRLVFGLGLLQVLGTIAVAVTLGFVLERWVHITWQASVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDL
AVVPLLIVIAALGGSSKDLVSSLGVAAIKIVVALSLLLIVGQRFMTRWFNVVARRRSQELFILNLLLVTLGCAFITDKFG
LSLALGAFIAGMLIAETPYRHQVEEDIKPFRDVLLGLFFITTGMLLNPRVIWEHPLIVLGFLVGPILLKAVMITGLSRLF
GASPGVAMRTGIGLAQAGEFGFVLLNLILDKHLVDATLLQAILAAMLLSMLAAPFLIQNADRIVLRLSSTEWMMQSLQMT
RIATQSLKQSGHVIICGYGRAGQNLARMLEHEGLSYVALDLDPDRVAAAAAAGESVVFGDAGRRESLLAAGIHRAAAIAI
TYANTPSALRVLHNIHELEPTLPVIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVVMGVPMRRVVRRVEEMRD
ERYALLRGYFHGADDVDDDDGHEQVRLQSVPVDENSDAVGRTLAELGLFEVGVEVTAIRRHGIRGVEPDPSTKLRSSDIV
VLRGLPEQLAEAEERLSKHRRAGAAA

Sequences:

>Translated_666_residues
MISPLEMTLFLLLASVAGVVTFRFLNLPPMLGYLTVGIVVGPHAFGLIPDSAGAQNLAEFGVVFLMFSIGLEFSLAKLRS
MRRLVFGLGLLQVLGTIAVAVTLGFVLERWVHITWQASVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDL
AVVPLLIVIAALGGSSKDLVSSLGVAAIKIVVALSLLLIVGQRFMTRWFNVVARRRSQELFILNLLLVTLGCAFITDKFG
LSLALGAFIAGMLIAETPYRHQVEEDIKPFRDVLLGLFFITTGMLLNPRVIWEHPLIVLGFLVGPILLKAVMITGLSRLF
GASPGVAMRTGIGLAQAGEFGFVLLNLILDKHLVDATLLQAILAAMLLSMLAAPFLIQNADRIVLRLSSTEWMMQSLQMT
RIATQSLKQSGHVIICGYGRAGQNLARMLEHEGLSYVALDLDPDRVAAAAAAGESVVFGDAGRRESLLAAGIHRAAAIAI
TYANTPSALRVLHNIHELEPTLPVIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVVMGVPMRRVVRRVEEMRD
ERYALLRGYFHGADDVDDDDGHEQVRLQSVPVDENSDAVGRTLAELGLFEVGVEVTAIRRHGIRGVEPDPSTKLRSSDIV
VLRGLPEQLAEAEERLSKHRRAGAAA
>Mature_666_residues
MISPLEMTLFLLLASVAGVVTFRFLNLPPMLGYLTVGIVVGPHAFGLIPDSAGAQNLAEFGVVFLMFSIGLEFSLAKLRS
MRRLVFGLGLLQVLGTIAVAVTLGFVLERWVHITWQASVALGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDL
AVVPLLIVIAALGGSSKDLVSSLGVAAIKIVVALSLLLIVGQRFMTRWFNVVARRRSQELFILNLLLVTLGCAFITDKFG
LSLALGAFIAGMLIAETPYRHQVEEDIKPFRDVLLGLFFITTGMLLNPRVIWEHPLIVLGFLVGPILLKAVMITGLSRLF
GASPGVAMRTGIGLAQAGEFGFVLLNLILDKHLVDATLLQAILAAMLLSMLAAPFLIQNADRIVLRLSSTEWMMQSLQMT
RIATQSLKQSGHVIICGYGRAGQNLARMLEHEGLSYVALDLDPDRVAAAAAAGESVVFGDAGRRESLLAAGIHRAAAIAI
TYANTPSALRVLHNIHELEPTLPVIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHTLVVMGVPMRRVVRRVEEMRD
ERYALLRGYFHGADDVDDDDGHEQVRLQSVPVDENSDAVGRTLAELGLFEVGVEVTAIRRHGIRGVEPDPSTKLRSSDIV
VLRGLPEQLAEAEERLSKHRRAGAAA

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=343, Percent_Identity=27.9883381924198, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1789749, Length=542, Percent_Identity=34.6863468634686, Blast_Score=253, Evalue=3e-68,
Organism=Escherichia coli, GI1786232, Length=515, Percent_Identity=33.7864077669903, Blast_Score=243, Evalue=4e-65,
Organism=Escherichia coli, GI1786685, Length=534, Percent_Identity=29.0262172284644, Blast_Score=195, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 71980; Mature: 71980

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISPLEMTLFLLLASVAGVVTFRFLNLPPMLGYLTVGIVVGPHAFGLIPDSAGAQNLAEF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
GVVFLMFSIGLEFSLAKLRSMRRLVFGLGLLQVLGTIAVAVTLGFVLERWVHITWQASVA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHH
LGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLIVIAALGGSSKDLV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SSLGVAAIKIVVALSLLLIVGQRFMTRWFNVVARRRSQELFILNLLLVTLGCAFITDKFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LSLALGAFIAGMLIAETPYRHQVEEDIKPFRDVLLGLFFITTGMLLNPRVIWEHPLIVLG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEECCCHHHHHH
FLVGPILLKAVMITGLSRLFGASPGVAMRTGIGLAQAGEFGFVLLNLILDKHLVDATLLQ
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHH
AILAAMLLSMLAAPFLIQNADRIVLRLSSTEWMMQSLQMTRIATQSLKQSGHVIICGYGR
HHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
AGQNLARMLEHEGLSYVALDLDPDRVAAAAAAGESVVFGDAGRRESLLAAGIHRAAAIAI
CCHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHEEEE
TYANTPSALRVLHNIHELEPTLPVIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHT
EECCCHHHHHHHHHHHHHCCCCHHEEEECCHHHHHHHHHCCHHHHHHHHHCCCCEEEECC
LVVMGVPMRRVVRRVEEMRDERYALLRGYFHGADDVDDDDGHEQVRLQSVPVDENSDAVG
CEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHH
RTLAELGLFEVGVEVTAIRRHGIRGVEPDPSTKLRSSDIVVLRGLPEQLAEAEERLSKHR
HHHHHHHHHHHCHHHEEHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
RAGAAA
HCCCCC
>Mature Secondary Structure
MISPLEMTLFLLLASVAGVVTFRFLNLPPMLGYLTVGIVVGPHAFGLIPDSAGAQNLAEF
CCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHH
GVVFLMFSIGLEFSLAKLRSMRRLVFGLGLLQVLGTIAVAVTLGFVLERWVHITWQASVA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHH
LGGALAMSSTAIVSKMLAERLEIETEHGRNIFGVLLFQDLAVVPLLIVIAALGGSSKDLV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SSLGVAAIKIVVALSLLLIVGQRFMTRWFNVVARRRSQELFILNLLLVTLGCAFITDKFG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LSLALGAFIAGMLIAETPYRHQVEEDIKPFRDVLLGLFFITTGMLLNPRVIWEHPLIVLG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEECCCHHHHHH
FLVGPILLKAVMITGLSRLFGASPGVAMRTGIGLAQAGEFGFVLLNLILDKHLVDATLLQ
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHH
AILAAMLLSMLAAPFLIQNADRIVLRLSSTEWMMQSLQMTRIATQSLKQSGHVIICGYGR
HHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCC
AGQNLARMLEHEGLSYVALDLDPDRVAAAAAAGESVVFGDAGRRESLLAAGIHRAAAIAI
CCHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHEEEE
TYANTPSALRVLHNIHELEPTLPVIVRTVDDADLEKLLAAGATEVIPEIVEGSLMLASHT
EECCCHHHHHHHHHHHHHCCCCHHEEEECCHHHHHHHHHCCHHHHHHHHHCCCCEEEECC
LVVMGVPMRRVVRRVEEMRDERYALLRGYFHGADDVDDDDGHEQVRLQSVPVDENSDAVG
CEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCHHH
RTLAELGLFEVGVEVTAIRRHGIRGVEPDPSTKLRSSDIVVLRGLPEQLAEAEERLSKHR
HHHHHHHHHHHCHHHEEHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHH
RAGAAA
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA