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The map label for this gene is 91781658

Identifier: 91781658

GI number: 91781658

Start: 309285

End: 310574

Strand: Direct

Name: 91781658

Synonym: Bxe_A4188

Alternate gene names: NA

Gene position: 309285-310574 (Clockwise)

Preceding gene: 91781657

Following gene: 91781659

Centisome position: 6.32

GC content: 62.87

Gene sequence:

>1290_bases
ATGGTTCCGCACCTAGTTACGGCGTTAAACGGCCCGCTGCTCGATCTTGAGCGGAAGATCCTCGACGCCACGCCCGCCAT
CGAACGCTGGTTCCGGCTCGAATGGCAGGAGCACACGCCGCCGTTCTACTGTTCCGTCGATCTGCGCAACGCGGGTTTCA
AGCTCGCGCCGGTCGACACCAATCTGTTTCCCGGCGCATTCAACAATCTGCCGCAGGAAGTGCTGCCGCTCGCCGTGCAG
GCCGCGATGGCCTCGATCGAGAAGATCTGCCCGGACGCCAAGAACCTGCTGGTGATTCCCGAGCGCCACACGCGCAACGC
GTTTTATCTGGAAAACGTCGCCCGTCTGGCCGCGATCATGCGCCAGGCCGGCTTGAACGTGCGCTTCGGCACGCTCGACG
AAACCATCAACGGGCCCGTCACGATCGCGCTCTCCGACGGCCAGAAACTCGTGCTCGAGCCGCTCGAGCGTTCGCCGCGG
CGCCTTGGTCTGAAGAATTTCGACCCGTGCTCGATTCTGCTGAACAACGATCTGTCGGGCGGCATCCCGCCAGTGCTCGA
AAATCTGCACGAGCAGTATCTGCTGCCGCCGCTGCATGCCGGCTGGGCCGTGCGCCGCAAATCCACGCATTTTTCCTGCT
ATGACGACGTCGCGAAGAAGTTCGCGAAGATGGTCGAGATCGATCCGTGGATGATCAATCCGTACTTCGCGCATGTGGAA
GGCGTCGATTTCCAGGAACGCACCGGCGAAGAAGCGTTAGCGGATGCGATCGACGGCGTGCTGAAGAAGATCGCCAAGAA
GTATCGCGAGTACGGCATCTCCGAGAAACCGTATGTCGTCATCAAGTCGGATGCGGGCACCTATGGCATGGGCGTGATGA
CCGTGCACGACGCGTCGGAAGTGGCCGCGCTCACCAAGCGCGAACGCGCCAAGATGGCGGCGACCAAAGACGGCCTGGAA
GTGCACGACGTGATCGTGCAGGAAGGCGTGTACACCTTCGAGCGCATCGGCGAAGAAGTGGCCGAGCCGGTCGTGTACAT
GATCGACCGCTATGTGGTGGGCGGCTTCTACCGCGTGCACGGCAGCCGCGAACGCGATCAGAATCTGAACGCGCCGGGCA
TGCACTTCGTGCCGCTCGGCTTCGAGCACACGGCGCTGCCCGACGCGCACGCCAAGCCCGGCGCGGCGCCGCCGAACCGC
TTCTACATGTACGGCGTGGTGGCGCGGCTCGGGCTGCTGGCCGCATCGGTCGAACTGGAAAAGACCGATCCGGAAGCGAT
CCAGGTTTAA

Upstream 100 bases:

>100_bases
AGCGAGAATGCATTCGCGTCCGCTTGCGAATGGAGAAACCGTCCCCAAATGGACAAAGCATTTGCTCTACAATCTTTTTT
CTCCAGTCTGCGAGTGATCA

Downstream 100 bases:

>100_bases
GCCACCCTGGGTGGGCGATGGCGGCATGCGTGCCGCCATCGCCATTTCCAACGACTATATTTACGATCAATGCCAAGCCC
ATCAGGGCGCACCGGGACCT

Product: putative glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Glutamate-Cysteine Ligase GshA; Glutamate-Cysteine Ligase-Related Protein; Glutathione Synthetase; GshA Protein

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGAFNNLPQEVLPLAVQ
AAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLAAIMRQAGLNVRFGTLDETINGPVTIALSDGQKLVLEPLERSPR
RLGLKNFDPCSILLNNDLSGGIPPVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVEIDPWMINPYFAHVE
GVDFQERTGEEALADAIDGVLKKIAKKYREYGISEKPYVVIKSDAGTYGMGVMTVHDASEVAALTKRERAKMAATKDGLE
VHDVIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVHGSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNR
FYMYGVVARLGLLAASVELEKTDPEAIQV

Sequences:

>Translated_429_residues
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGAFNNLPQEVLPLAVQ
AAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLAAIMRQAGLNVRFGTLDETINGPVTIALSDGQKLVLEPLERSPR
RLGLKNFDPCSILLNNDLSGGIPPVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVEIDPWMINPYFAHVE
GVDFQERTGEEALADAIDGVLKKIAKKYREYGISEKPYVVIKSDAGTYGMGVMTVHDASEVAALTKRERAKMAATKDGLE
VHDVIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVHGSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNR
FYMYGVVARLGLLAASVELEKTDPEAIQV
>Mature_429_residues
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDTNLFPGAFNNLPQEVLPLAVQ
AAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLAAIMRQAGLNVRFGTLDETINGPVTIALSDGQKLVLEPLERSPR
RLGLKNFDPCSILLNNDLSGGIPPVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVEIDPWMINPYFAHVE
GVDFQERTGEEALADAIDGVLKKIAKKYREYGISEKPYVVIKSDAGTYGMGVMTVHDASEVAALTKRERAKMAATKDGLE
VHDVIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVHGSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNR
FYMYGVVARLGLLAASVELEKTDPEAIQV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 47746; Mature: 47746

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPQEVLPLAVQAAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLAAIM
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGTLDETINGPVTIALSDGQKLVLEPLERSPRRLGLKNFDPCSILLNNDLSG
HHCCCCEEECCCHHCCCCCEEEEECCCCEEHHHHHHCCHHHCCCCCCCCEEEEECCCCCC
GIPPVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVEIDPWMINPYFAHVE
CCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEC
GVDFQERTGEEALADAIDGVLKKIAKKYREYGISEKPYVVIKSDAGTYGMGVMTVHDASE
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCHHH
VAALTKRERAKMAATKDGLEVHDVIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
GSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNRFYMYGVVARLGLLAASVELE
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEE
KTDPEAIQV
CCCCHHCCC
>Mature Secondary Structure
MVPHLVTALNGPLLDLERKILDATPAIERWFRLEWQEHTPPFYCSVDLRNAGFKLAPVDT
CCCCHHHHHCCCHHHHHHHHHHCCHHHHHHHHCCHHCCCCCEEEEEEECCCCEEEEECCC
NLFPGAFNNLPQEVLPLAVQAAMASIEKICPDAKNLLVIPERHTRNAFYLENVARLAAIM
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
RQAGLNVRFGTLDETINGPVTIALSDGQKLVLEPLERSPRRLGLKNFDPCSILLNNDLSG
HHCCCCEEECCCHHCCCCCEEEEECCCCEEHHHHHHCCHHHCCCCCCCCEEEEECCCCCC
GIPPVLENLHEQYLLPPLHAGWAVRRKSTHFSCYDDVAKKFAKMVEIDPWMINPYFAHVE
CCCHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCEEEEC
GVDFQERTGEEALADAIDGVLKKIAKKYREYGISEKPYVVIKSDAGTYGMGVMTVHDASE
CCCHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCHHH
VAALTKRERAKMAATKDGLEVHDVIVQEGVYTFERIGEEVAEPVVYMIDRYVVGGFYRVH
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEC
GSRERDQNLNAPGMHFVPLGFEHTALPDAHAKPGAAPPNRFYMYGVVARLGLLAASVELE
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEE
KTDPEAIQV
CCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA