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The map label for this gene is amtB [H]

Identifier: 91781657

GI number: 91781657

Start: 307547

End: 309046

Strand: Direct

Name: amtB [H]

Synonym: Bxe_A4189

Alternate gene names: 91781657

Gene position: 307547-309046 (Clockwise)

Preceding gene: 91781656

Following gene: 91781658

Centisome position: 6.28

GC content: 65.53

Gene sequence:

>1500_bases
ATGCGCAAATTATTGATGTCCTTGCTGATGGCCGGTTCGCTGCTCGCGGGCGGTATCGGCGCTGCTCTCGCGGACGACGC
GTCCGCTCCCGCAGCCGCCTCGGCTCCCGATGCGACGGCGAGCGCCCCGGCACCGGACGCTTCGGCGGCACCCGCTGCCG
CCGCCGCTTCGGCACCCGCCGCCGATGCATCCGCCGCACCGGCTGCCAGCGCGGCCGCCGCACCTGCTGCATCGGACGCC
GCGCCGGCCGCGCCGACCGCACCGTTCTCCGTCGATTCCTCGAAGATCAATTCCGGCGACACCGCCTGGATGCTGACTTC
CACCGCCCTCGTGCTGTTCATGACGATCCCGGGTCTGGCACTGTTCTACGGCGGCATGGTCCGCAAGAAGAACGTGCTCG
CGATCCTGATGCAAAGCTTCGCGATCACCTGCGTGGTGACGATCGTCTGGATCGTGATCGGCTACAGCCTTGCCTTCACG
CCGGGCAGTTCGTTCATCGGCGGCTTCTCGCGCGTCTTCATGGCGGGCATGAGCTACATCAAGGGCGACAAGGCCACGAC
GCTGACCGTCAGCCATCTGGCCCCGACGATCCCGGAAACGGTCTATTGCGTCTATCAGATGACGTTCGCGATCATCACCC
CGGCACTGATCACGGGCGCGTTTGCCGACCGTATGAAGTTCTCGGCGATGCTGGTGTTCATGACGCTGTGGTCGATCATC
GTCTACTCGCCGATCGCACACATGGTCTGGGAACCGACCGGCTGGCTGGCTAGCGCGGGCATCCTCGACTTCGCGGGCGG
CACGGTGGTGCACATCAACGCCGGTATTGCGGCGCTGGTCTGCGCGCTGGTCCTCGGCAAGCGTATCGGCTACGGCAAGG
AAGCGATGGCGCCGCACAACCTGACGCTCACGCTGATCGGCGGCGCCATGCTGTGGGTGGGCTGGTTCGGCTTCAACGCG
GGTTCGGCAGTGGCGGCTGACGGCCGTGCCGGTTTCGCGATGTTCGCAACCCAGGTGGCAACTGCCGCGGCAGCACTCGC
CTGGATGTTCGCTGAATGGGCCGCCAAGGGTAAGCCGTCGGTGCTCGGTATCGTGTCGGGCGCAGTGGCAGGTCTGGTGG
CGATCACGCCGGCTTCGGGCTTCGTCGGTACGCTCGGCTCGCTGGTGATCGGTATCGTGGCAGGCGTGGTCTGCTTCTGG
TCGGCAACGTGGCTCAAGCACAAGCTCGGCTACGACGATTCGCTCGACGCGTTCGGCGTGCACTGCATCGGCGGTATCGT
GGGTGCGCTCCTGACCGGCGTGTTCGCGGTCAAGGACATCGGCGGCGCGGACGGCAGCGTCATCCTGCAAGCCAAGGGCG
TGCTGACGACGCTGATCTACAGCGGCGTGGTGAGCTACATCCTGCTGAAGATCATCGACATGGTGATGGGCATTCGCGTG
ACGGAAGAACAGGAACGCGAAGGTCTGGACGTGAGCCTGCACGGCGAACACGTCGAGTAA

Upstream 100 bases:

>100_bases
CGATCGAGCAGGTGATCCGGATTCGCACCGGGGAGACCGGCGCGGACGCTCTGTAATACCAAAAGATAGCGACACAGATA
GCGATAAGAGGAAACGAAAG

Downstream 100 bases:

>100_bases
GCAACGTACCGCGCGAGCCCGCGCTCCCGGGCTCGCCGGATCAGAAATAAGCGCAGCGACTTTGGCCCGCCTTCATGGCG
GGCTTTTTTCTTGCGCGCTA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MRKLLMSLLMAGSLLAGGIGAALADDASAPAAASAPDATASAPAPDASAAPAAAAASAPAADASAAPAASAAAAPAASDA
APAAPTAPFSVDSSKINSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKNVLAILMQSFAITCVVTIVWIVIGYSLAFT
PGSSFIGGFSRVFMAGMSYIKGDKATTLTVSHLAPTIPETVYCVYQMTFAIITPALITGAFADRMKFSAMLVFMTLWSII
VYSPIAHMVWEPTGWLASAGILDFAGGTVVHINAGIAALVCALVLGKRIGYGKEAMAPHNLTLTLIGGAMLWVGWFGFNA
GSAVAADGRAGFAMFATQVATAAAALAWMFAEWAAKGKPSVLGIVSGAVAGLVAITPASGFVGTLGSLVIGIVAGVVCFW
SATWLKHKLGYDDSLDAFGVHCIGGIVGALLTGVFAVKDIGGADGSVILQAKGVLTTLIYSGVVSYILLKIIDMVMGIRV
TEEQEREGLDVSLHGEHVE

Sequences:

>Translated_499_residues
MRKLLMSLLMAGSLLAGGIGAALADDASAPAAASAPDATASAPAPDASAAPAAAAASAPAADASAAPAASAAAAPAASDA
APAAPTAPFSVDSSKINSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKNVLAILMQSFAITCVVTIVWIVIGYSLAFT
PGSSFIGGFSRVFMAGMSYIKGDKATTLTVSHLAPTIPETVYCVYQMTFAIITPALITGAFADRMKFSAMLVFMTLWSII
VYSPIAHMVWEPTGWLASAGILDFAGGTVVHINAGIAALVCALVLGKRIGYGKEAMAPHNLTLTLIGGAMLWVGWFGFNA
GSAVAADGRAGFAMFATQVATAAAALAWMFAEWAAKGKPSVLGIVSGAVAGLVAITPASGFVGTLGSLVIGIVAGVVCFW
SATWLKHKLGYDDSLDAFGVHCIGGIVGALLTGVFAVKDIGGADGSVILQAKGVLTTLIYSGVVSYILLKIIDMVMGIRV
TEEQEREGLDVSLHGEHVE
>Mature_499_residues
MRKLLMSLLMAGSLLAGGIGAALADDASAPAAASAPDATASAPAPDASAAPAAAAASAPAADASAAPAASAAAAPAASDA
APAAPTAPFSVDSSKINSGDTAWMLTSTALVLFMTIPGLALFYGGMVRKKNVLAILMQSFAITCVVTIVWIVIGYSLAFT
PGSSFIGGFSRVFMAGMSYIKGDKATTLTVSHLAPTIPETVYCVYQMTFAIITPALITGAFADRMKFSAMLVFMTLWSII
VYSPIAHMVWEPTGWLASAGILDFAGGTVVHINAGIAALVCALVLGKRIGYGKEAMAPHNLTLTLIGGAMLWVGWFGFNA
GSAVAADGRAGFAMFATQVATAAAALAWMFAEWAAKGKPSVLGIVSGAVAGLVAITPASGFVGTLGSLVIGIVAGVVCFW
SATWLKHKLGYDDSLDAFGVHCIGGIVGALLTGVFAVKDIGGADGSVILQAKGVLTTLIYSGVVSYILLKIIDMVMGIRV
TEEQEREGLDVSLHGEHVE

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=440, Percent_Identity=48.6363636363636, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17550182, Length=449, Percent_Identity=28.0623608017817, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=27.4599542334096, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=429, Percent_Identity=24.4755244755245, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI133931100, Length=451, Percent_Identity=22.8381374722838, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324187, Length=435, Percent_Identity=35.1724137931034, Blast_Score=231, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6325396, Length=427, Percent_Identity=33.4894613583138, Blast_Score=221, Evalue=3e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=414, Percent_Identity=31.6425120772947, Blast_Score=210, Evalue=3e-55,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=25.8293838862559, Blast_Score=109, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50692; Mature: 50692

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLLMSLLMAGSLLAGGIGAALADDASAPAAASAPDATASAPAPDASAAPAAAAASAPA
CHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
ADASAAPAASAAAAPAASDAAPAAPTAPFSVDSSKINSGDTAWMLTSTALVLFMTIPGLA
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LFYGGMVRKKNVLAILMQSFAITCVVTIVWIVIGYSLAFTPGSSFIGGFSRVFMAGMSYI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHH
KGDKATTLTVSHLAPTIPETVYCVYQMTFAIITPALITGAFADRMKFSAMLVFMTLWSII
CCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYSPIAHMVWEPTGWLASAGILDFAGGTVVHINAGIAALVCALVLGKRIGYGKEAMAPHN
HHHHHHHHHCCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCC
LTLTLIGGAMLWVGWFGFNAGSAVAADGRAGFAMFATQVATAAAALAWMFAEWAAKGKPS
EEEEEEHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLGIVSGAVAGLVAITPASGFVGTLGSLVIGIVAGVVCFWSATWLKHKLGYDDSLDAFGV
EEHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
HCIGGIVGALLTGVFAVKDIGGADGSVILQAKGVLTTLIYSGVVSYILLKIIDMVMGIRV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEEQEREGLDVSLHGEHVE
CCHHHHCCCCEEECCCCCC
>Mature Secondary Structure
MRKLLMSLLMAGSLLAGGIGAALADDASAPAAASAPDATASAPAPDASAAPAAAAASAPA
CHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCC
ADASAAPAASAAAAPAASDAAPAAPTAPFSVDSSKINSGDTAWMLTSTALVLFMTIPGLA
CCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LFYGGMVRKKNVLAILMQSFAITCVVTIVWIVIGYSLAFTPGSSFIGGFSRVFMAGMSYI
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHH
KGDKATTLTVSHLAPTIPETVYCVYQMTFAIITPALITGAFADRMKFSAMLVFMTLWSII
CCCCCEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VYSPIAHMVWEPTGWLASAGILDFAGGTVVHINAGIAALVCALVLGKRIGYGKEAMAPHN
HHHHHHHHHCCCCCHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCC
LTLTLIGGAMLWVGWFGFNAGSAVAADGRAGFAMFATQVATAAAALAWMFAEWAAKGKPS
EEEEEEHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VLGIVSGAVAGLVAITPASGFVGTLGSLVIGIVAGVVCFWSATWLKHKLGYDDSLDAFGV
EEHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
HCIGGIVGALLTGVFAVKDIGGADGSVILQAKGVLTTLIYSGVVSYILLKIIDMVMGIRV
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TEEQEREGLDVSLHGEHVE
CCHHHHCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]