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The map label for this gene is lipA [H]

Identifier: 91781634

GI number: 91781634

Start: 283967

End: 284971

Strand: Direct

Name: lipA [H]

Synonym: Bxe_A4212

Alternate gene names: 91781634

Gene position: 283967-284971 (Clockwise)

Preceding gene: 91781633

Following gene: 91781635

Centisome position: 5.8

GC content: 61.19

Gene sequence:

>1005_bases
ATGACTGACGTTACCGCGAACCTCGCAGCTTCTCCCGCTCCTGATGCCGTGCCGGCTCCCGCCGCCTACGACGCCACCGC
GAAGCAGAAAGCGCAAGCCAAGACCGCCCGTATCCCCATCAAGGTCATCCCGATCGAAAAGCTGAAGAAGCCGGACTGGA
TTCGCGTGAAAGCGGCCACCGGCAATTCGCGCTTCTACGAAATCAAGCAGATTCTGCGCGAACATAACCTGCATACGGTG
TGTGAAGAAGCGAGTTGCCCGAACATTGGCGAGTGTTTCGGCAAGGGCACCGCGACCTTCATGATCATGGGCGACAAATG
CACGCGCCGCTGCCCGTTCTGCGACGTCGGCCACGGTCGCCCGGATCCGCTCGACGCCGACGAACCAGGCAATCTGGCGC
GCACGATCGCCGCGCTCAAGCTGAAGTACGTGGTGATCACCAGCGTTGACCGCGACGATTTGCGTGACGGCGGCGCGGCT
CACTTCGTGGAATGTATCCGTCAGACGCGCGAGCTTTCGCCGCAGACGCGCATCGAAATCCTGACGCCGGACTTCCGTGG
CCGTCTGGATCGTGCGCTCGGTATTCTGAATGCCGCACCGCCCGATGTGATGAATCACAACCTCGAAACGGTGCCGCGCC
TGTACAAGGAAGCGCGTCCGGGTTCGGACTATGCGCATTCGCTGAAGCTGCTGAAGGACTTCAAGGCACTGCATCCTGAC
GTGGCCACCAAGTCCGGTTTGATGGTCGGCCTCGGCGAAACCGAAGAAGAGATTCTGCAAGTGATGCGCGACCTGCGCGA
ACACGACGTGGATATGCTGACGATCGGCCAGTACCTGCAGCCGTCGGAACACCATCTGCCGGTGCGCTCGTACGTGCACC
CGGACACGTTCAGGATGTACGAGGAAGAAGCGTACAAGATGGGTTTCACGCACGCAGCCGTGGGAGCGATGGTTCGTTCG
AGCTACCACGCCGACTTGCAGGCGCATGGAGCCGGCGTGGTCTGA

Upstream 100 bases:

>100_bases
CGTACCTTTGCAGCATGTCTCACCGCAAACCTCGACGGCAGTCCCGCGGCCGTCGCCCAACCGCAGGCCGGTGCACTTAC
CGCCTGACTGGACCGAACGA

Downstream 100 bases:

>100_bases
GTATCTTCGCTAGCTGGCTGTCAGATTGGGAAAAGCCTGCATGTGTTGCAGGCTTTTCTCTTTGGGCGGCCAAACATTGA
GCGCTGGAATCACACGCGGT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MTDVTANLAASPAPDAVPAPAAYDATAKQKAQAKTARIPIKVIPIEKLKKPDWIRVKAATGNSRFYEIKQILREHNLHTV
CEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPGNLARTIAALKLKYVVITSVDRDDLRDGGAA
HFVECIRQTRELSPQTRIEILTPDFRGRLDRALGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPD
VATKSGLMVGLGETEEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVRSYVHPDTFRMYEEEAYKMGFTHAAVGAMVRS
SYHADLQAHGAGVV

Sequences:

>Translated_334_residues
MTDVTANLAASPAPDAVPAPAAYDATAKQKAQAKTARIPIKVIPIEKLKKPDWIRVKAATGNSRFYEIKQILREHNLHTV
CEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPGNLARTIAALKLKYVVITSVDRDDLRDGGAA
HFVECIRQTRELSPQTRIEILTPDFRGRLDRALGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPD
VATKSGLMVGLGETEEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVRSYVHPDTFRMYEEEAYKMGFTHAAVGAMVRS
SYHADLQAHGAGVV
>Mature_333_residues
TDVTANLAASPAPDAVPAPAAYDATAKQKAQAKTARIPIKVIPIEKLKKPDWIRVKAATGNSRFYEIKQILREHNLHTVC
EEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGRPDPLDADEPGNLARTIAALKLKYVVITSVDRDDLRDGGAAH
FVECIRQTRELSPQTRIEILTPDFRGRLDRALGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPDV
ATKSGLMVGLGETEEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVRSYVHPDTFRMYEEEAYKMGFTHAAVGAMVRSS
YHADLQAHGAGVV

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=286, Percent_Identity=47.9020979020979, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=49.2, Blast_Score=241, Evalue=8e-64,
Organism=Escherichia coli, GI1786846, Length=317, Percent_Identity=62.4605678233439, Blast_Score=415, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32564533, Length=254, Percent_Identity=45.6692913385827, Blast_Score=224, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=289, Percent_Identity=44.9826989619377, Blast_Score=245, Evalue=6e-66,
Organism=Drosophila melanogaster, GI221513272, Length=286, Percent_Identity=46.5034965034965, Blast_Score=259, Evalue=1e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 36987; Mature: 36856

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVTANLAASPAPDAVPAPAAYDATAKQKAQAKTARIPIKVIPIEKLKKPDWIRVKAAT
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEEEC
GNSRFYEIKQILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGR
CCCHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCC
PDPLDADEPGNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVECIRQTRELSPQTRIEI
CCCCCCCCCCHHHHHHHHHHEEEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
LTPDFRGRLDRALGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPD
ECCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
VATKSGLMVGLGETEEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVRSYVHPDTFRMY
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCHHHHCCCHHHHHH
EEEAYKMGFTHAAVGAMVRSSYHADLQAHGAGVV
HHHHHHHCCHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure 
TDVTANLAASPAPDAVPAPAAYDATAKQKAQAKTARIPIKVIPIEKLKKPDWIRVKAAT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECHHHCCCCCEEEEEEEC
GNSRFYEIKQILREHNLHTVCEEASCPNIGECFGKGTATFMIMGDKCTRRCPFCDVGHGR
CCCHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCC
PDPLDADEPGNLARTIAALKLKYVVITSVDRDDLRDGGAAHFVECIRQTRELSPQTRIEI
CCCCCCCCCCHHHHHHHHHHEEEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEE
LTPDFRGRLDRALGILNAAPPDVMNHNLETVPRLYKEARPGSDYAHSLKLLKDFKALHPD
ECCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
VATKSGLMVGLGETEEEILQVMRDLREHDVDMLTIGQYLQPSEHHLPVRSYVHPDTFRMY
CCCCCCEEEECCCCHHHHHHHHHHHHHCCCCEEEHHHHCCCCCCCCCHHHHCCCHHHHHH
EEEAYKMGFTHAAVGAMVRSSYHADLQAHGAGVV
HHHHHHHCCHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA