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The map label for this gene is lipB [H]

Identifier: 91781633

GI number: 91781633

Start: 283228

End: 283953

Strand: Direct

Name: lipB [H]

Synonym: Bxe_A4213

Alternate gene names: 91781633

Gene position: 283228-283953 (Clockwise)

Preceding gene: 91781632

Following gene: 91781634

Centisome position: 5.79

GC content: 64.88

Gene sequence:

>726_bases
ATGTGCGCCACCCCGGTTTCTCCGACTCCCTTACCCGCAACGGCACCGCTCGCGCTGCGCTGGCGTGGCACCGAACCCTA
CGAAGCCAGTTTCGAAGCCATGCGCGCGTTCACCGACGGGCGCACCGCCGAGACTCCCGACGAAATCTGGCTGGTCGAAC
ATCCTCCCGTTTTCACGCTAGGCCAGGCCGGCGACCCAGCGCACCTGCTGGCGGCTGACAGCGGTATTCCTCTGGTTAAA
GTCGACCGCGGCGGGCAGATTACGTATCACGGCCCCGGCCAGGTGGTTGCCTACCTGCTGCTCGATTTGCGCCGTCGCAA
ATTGATGGTGCGCGAGCTGGTTACGCGGATCGAGCAAGCCGTGATCGACACGCTTGCGGCGTATAATCTCGCCGGAGAAC
GAAAGGCGGGCGCCCCCGGTATCTATGTGGCGCCCGGGCCGGCCGCCGGGTCGCACGCCGGCGCCAAGATCGCGGCGCTG
GGTTTGAAAATCCGCAACGGCTGCAGCTATCACGGCGTGAGTCTGAACGTGAACATGGATTTGCGGCCGTTTCTCGCCAT
CAATCCATGCGGCTACGCGGGGCTCGAAACAGTCGATATGGCAACGCTAGGCGTTACCGCCGGCTGGAACGACGTGGCCC
GTACCTTTGCAGCATGTCTCACCGCAAACCTCGACGGCAGTCCCGCGGCCGTCGCCCAACCGCAGGCCGGTGCACTTACC
GCCTGA

Upstream 100 bases:

>100_bases
ACATCTGGTATGACCGGCGCGGCCGCGCTATCTTGACTCGACGCTGCGGACCTCCCCATTCCCGGCGGGGATCCGCAGAT
TTCGGTAAACTGACGGGATC

Downstream 100 bases:

>100_bases
CTGGACCGAACGAATGACTGACGTTACCGCGAACCTCGCAGCTTCTCCCGCTCCTGATGCCGTGCCGGCTCCCGCCGCCT
ACGACGCCACCGCGAAGCAG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MCATPVSPTPLPATAPLALRWRGTEPYEASFEAMRAFTDGRTAETPDEIWLVEHPPVFTLGQAGDPAHLLAADSGIPLVK
VDRGGQITYHGPGQVVAYLLLDLRRRKLMVRELVTRIEQAVIDTLAAYNLAGERKAGAPGIYVAPGPAAGSHAGAKIAAL
GLKIRNGCSYHGVSLNVNMDLRPFLAINPCGYAGLETVDMATLGVTAGWNDVARTFAACLTANLDGSPAAVAQPQAGALT
A

Sequences:

>Translated_241_residues
MCATPVSPTPLPATAPLALRWRGTEPYEASFEAMRAFTDGRTAETPDEIWLVEHPPVFTLGQAGDPAHLLAADSGIPLVK
VDRGGQITYHGPGQVVAYLLLDLRRRKLMVRELVTRIEQAVIDTLAAYNLAGERKAGAPGIYVAPGPAAGSHAGAKIAAL
GLKIRNGCSYHGVSLNVNMDLRPFLAINPCGYAGLETVDMATLGVTAGWNDVARTFAACLTANLDGSPAAVAQPQAGALT
A
>Mature_241_residues
MCATPVSPTPLPATAPLALRWRGTEPYEASFEAMRAFTDGRTAETPDEIWLVEHPPVFTLGQAGDPAHLLAADSGIPLVK
VDRGGQITYHGPGQVVAYLLLDLRRRKLMVRELVTRIEQAVIDTLAAYNLAGERKAGAPGIYVAPGPAAGSHAGAKIAAL
GLKIRNGCSYHGVSLNVNMDLRPFLAINPCGYAGLETVDMATLGVTAGWNDVARTFAACLTANLDGSPAAVAQPQAGALT
A

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI87081767, Length=210, Percent_Identity=50.9523809523809, Blast_Score=210, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6323268, Length=181, Percent_Identity=30.939226519337, Blast_Score=82, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21355483, Length=220, Percent_Identity=29.5454545454545, Blast_Score=77, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 25193; Mature: 25193

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCATPVSPTPLPATAPLALRWRGTEPYEASFEAMRAFTDGRTAETPDEIWLVEHPPVFTL
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEE
GQAGDPAHLLAADSGIPLVKVDRGGQITYHGPGQVVAYLLLDLRRRKLMVRELVTRIEQA
CCCCCCCEEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIDTLAAYNLAGERKAGAPGIYVAPGPAAGSHAGAKIAALGLKIRNGCSYHGVSLNVNMD
HHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCC
LRPFLAINPCGYAGLETVDMATLGVTAGWNDVARTFAACLTANLDGSPAAVAQPQAGALT
CCCEEEECCCCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCC
A
C
>Mature Secondary Structure
MCATPVSPTPLPATAPLALRWRGTEPYEASFEAMRAFTDGRTAETPDEIWLVEHPPVFTL
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEE
GQAGDPAHLLAADSGIPLVKVDRGGQITYHGPGQVVAYLLLDLRRRKLMVRELVTRIEQA
CCCCCCCEEEEECCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VIDTLAAYNLAGERKAGAPGIYVAPGPAAGSHAGAKIAALGLKIRNGCSYHGVSLNVNMD
HHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCC
LRPFLAINPCGYAGLETVDMATLGVTAGWNDVARTFAACLTANLDGSPAAVAQPQAGALT
CCCEEEECCCCCCCCCCHHHEEEEEECCHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA