Click here to switch to the map view.

The map label for this gene is bioB [H]

Identifier: 91781580

GI number: 91781580

Start: 228924

End: 230006

Strand: Reverse

Name: bioB [H]

Synonym: Bxe_A4266

Alternate gene names: 91781580

Gene position: 230006-228924 (Counterclockwise)

Preceding gene: 91781581

Following gene: 91781577

Centisome position: 4.7

GC content: 62.97

Gene sequence:

>1083_bases
ATGACGCAACTGAACATCGCTCCGGCGTCCACCGACACGGCCGCCGCCAGCAACAACAACGCCGCAGCCGGCACGAAGCA
GCTCGCGCGCTGGCGCGTGGCGGACATCGTCTCGTTGTACGAACTGCCGTTCAACGACCTGATGTTCCAGGCGCAGCAAA
CCCATCGCGAGCATTTCGACGCGAACACCGTGCAACTGTCCACGCTGCTGTCGATCAAGACCGGCGGCTGCGAGGAAGAT
TGCGCGTATTGTCCGCAGTCGGTGCATCACGACACGGGCCTGCAAGCCGACAAGCTGATGCCGGTCGACGAAGTGCTCGC
CGCCGCGAAGGTCGCCAAGGAAAACGGCGCGACGCGTTTCTGCATGGGCGCCGCGTGGCGCAACCCGAAAGACCGTCACC
TCGAACCGATCAAGGACATGATCCGCGGCGTGAAGGCAATGGGCCTTGAAACCTGCGTGACGCTCGGCATGCTCGAAACG
CATCAGGCGCAAGGGCTGCGCGAAGCGGGCCTCGACTACTACAACCACAATCTCGATACGTCGCCGGAGTTCTACGGTCA
GATCATTTCGACGCGCACGTATCAGGACCGCCTCGACACGCTCGAACGCGTGCGCGACGCGGGCATCAACGTATGCTGCG
GCGGGATCGTCGGTCTGGGCGAGTCGCGCCGTGAACGCGCGGGCCTGATCGCGCAACTGGCGAACATGGATCCGTATCCG
GAATCGGTGCCCATCAACAATCTGGTTCAGGTGGAAGGCACGCCGCTGACCGGCACCGAAGCGATCGACCCGTTCGAATT
CGTGCGCACGATCGCGATTGCGCGCATCACCATGCCGCGCGCAATGGTGCGTCTGTCGGCCGGCCGCGAACAAATGAACG
AAGCGTTGCAGGCCATGTGTTTCCTCGCGGGCGCGAACTCGATTTTCTACGGCGATCAATTGCTGACCACGAGCAACCCG
CAAGCCGAAGCCGACCGCAAACTGCTCGAACGTCTCGGCATTCGCGCCGAGGCCGCGCAGCAGATGCCACTCGATCAAAG
CGGCTGCGAACATGGCTGCGATAAACACGCTGCGCCGAACTGA

Upstream 100 bases:

>100_bases
GCAGCGGAGCAACTCGACATCAACCGGCTTCTGCAAACGCTGCGCCATGCGCATGCGCAGCGCTGAACCACCATCACATC
CATCCATGAGGATCGACGAC

Downstream 100 bases:

>100_bases
GCGCATGCGTCGCGTGCAGTAGAACACGCGCGATAAAAAAGGCTGCCTCGTTTTCCACGAGGCAGCCTTTTTACTTGTGA
AGCCGGAAATTACTTCTTGT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTQLNIAPASTDTAAASNNNAAAGTKQLARWRVADIVSLYELPFNDLMFQAQQTHREHFDANTVQLSTLLSIKTGGCEED
CAYCPQSVHHDTGLQADKLMPVDEVLAAAKVAKENGATRFCMGAAWRNPKDRHLEPIKDMIRGVKAMGLETCVTLGMLET
HQAQGLREAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIVGLGESRRERAGLIAQLANMDPYP
ESVPINNLVQVEGTPLTGTEAIDPFEFVRTIAIARITMPRAMVRLSAGREQMNEALQAMCFLAGANSIFYGDQLLTTSNP
QAEADRKLLERLGIRAEAAQQMPLDQSGCEHGCDKHAAPN

Sequences:

>Translated_360_residues
MTQLNIAPASTDTAAASNNNAAAGTKQLARWRVADIVSLYELPFNDLMFQAQQTHREHFDANTVQLSTLLSIKTGGCEED
CAYCPQSVHHDTGLQADKLMPVDEVLAAAKVAKENGATRFCMGAAWRNPKDRHLEPIKDMIRGVKAMGLETCVTLGMLET
HQAQGLREAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIVGLGESRRERAGLIAQLANMDPYP
ESVPINNLVQVEGTPLTGTEAIDPFEFVRTIAIARITMPRAMVRLSAGREQMNEALQAMCFLAGANSIFYGDQLLTTSNP
QAEADRKLLERLGIRAEAAQQMPLDQSGCEHGCDKHAAPN
>Mature_359_residues
TQLNIAPASTDTAAASNNNAAAGTKQLARWRVADIVSLYELPFNDLMFQAQQTHREHFDANTVQLSTLLSIKTGGCEEDC
AYCPQSVHHDTGLQADKLMPVDEVLAAAKVAKENGATRFCMGAAWRNPKDRHLEPIKDMIRGVKAMGLETCVTLGMLETH
QAQGLREAGLDYYNHNLDTSPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIVGLGESRRERAGLIAQLANMDPYPE
SVPINNLVQVEGTPLTGTEAIDPFEFVRTIAIARITMPRAMVRLSAGREQMNEALQAMCFLAGANSIFYGDQLLTTSNPQ
AEADRKLLERLGIRAEAAQQMPLDQSGCEHGCDKHAAPN

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=64.2622950819672, Blast_Score=428, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6321725, Length=344, Percent_Identity=47.093023255814, Blast_Score=326, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 39393; Mature: 39262

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLNIAPASTDTAAASNNNAAAGTKQLARWRVADIVSLYELPFNDLMFQAQQTHREHFD
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
ANTVQLSTLLSIKTGGCEEDCAYCPQSVHHDTGLQADKLMPVDEVLAAAKVAKENGATRF
CCCEEHHHHEEECCCCCCHHHHHCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEE
CMGAAWRNPKDRHLEPIKDMIRGVKAMGLETCVTLGMLETHQAQGLREAGLDYYNHNLDT
EEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCC
SPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIVGLGESRRERAGLIAQLANMDPYP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCC
ESVPINNLVQVEGTPLTGTEAIDPFEFVRTIAIARITMPRAMVRLSAGREQMNEALQAMC
CCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FLAGANSIFYGDQLLTTSNPQAEADRKLLERLGIRAEAAQQMPLDQSGCEHGCDKHAAPN
HHHCCCCEEECCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
TQLNIAPASTDTAAASNNNAAAGTKQLARWRVADIVSLYELPFNDLMFQAQQTHREHFD
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
ANTVQLSTLLSIKTGGCEEDCAYCPQSVHHDTGLQADKLMPVDEVLAAAKVAKENGATRF
CCCEEHHHHEEECCCCCCHHHHHCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCEE
CMGAAWRNPKDRHLEPIKDMIRGVKAMGLETCVTLGMLETHQAQGLREAGLDYYNHNLDT
EEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHCCCCCC
SPEFYGQIISTRTYQDRLDTLERVRDAGINVCCGGIVGLGESRRERAGLIAQLANMDPYP
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCC
ESVPINNLVQVEGTPLTGTEAIDPFEFVRTIAIARITMPRAMVRLSAGREQMNEALQAMC
CCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
FLAGANSIFYGDQLLTTSNPQAEADRKLLERLGIRAEAAQQMPLDQSGCEHGCDKHAAPN
HHHCCCCEEECCEEEECCCCCHHHHHHHHHHHCCHHHHHHHCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA