Click here to switch to the map view.

The map label for this gene is braC [H]

Identifier: 91781519

GI number: 91781519

Start: 161380

End: 162588

Strand: Direct

Name: braC [H]

Synonym: Bxe_A4327

Alternate gene names: 91781519

Gene position: 161380-162588 (Clockwise)

Preceding gene: 91781518

Following gene: 91781520

Centisome position: 3.3

GC content: 59.97

Gene sequence:

>1209_bases
GTGTTGGGGAAGCGGGGGCACAGGGCGATTACGTTGTTTTTGGGACCGAAACTGGAGACTTACATGAACATCAAGATTCA
AAAGCTGTTGCCGATCAGCGCTGCGGCGATGCTGTTCGCAACGTTGGCAACTTCCGCGGCAGCTGACCAGGTCGTCAAGA
TCGGCCACGTTGCACCGCTGACTGGCGGTATCGCTCACCTGGGTAAAGACAACGAAAACGGTGCACGTCTGGCAGTTGAA
GAAATCAACGCCAAAGGCCTGACGATCGGCGGCCAGAAGATCACGCTGCAACTGGACGCACAAGACGACGCGGCCGACCC
GCGCACCGCAACGCAAGTCGCGCAGAAGCTGGTCGACGACAAGGTCGTCGCCGTGGTCGGCCACTTGAACTCGGGTACGT
CGATCCCGGCTTCGAAGATCTATAGCGATGCCGGCATCGTGCAGATCTCGCCGTCGGCAACGAACCCGGCCTACACGCAG
CAAGGTTTCAAGACCACGTACCGCGTCGTCGCGACCGATGCGCAACAAGGTCCGGCACTGGCTAACTACGCAGCGAAGGG
TCTGAAAGTGAAGAGCGTCGCGATCGTCGACGACTCGACCGCTTACGGCCAGGGTCTGGCAAACGAATTCGAAAAGACGG
CGAAATCGCTGGGCCTGAACGTGATGTCGCATGACGCGACGAACGACAAGGCTGTCGACTTCCGCGCGATTCTGACGAAG
ATCAAGGGCGAAAACCCGGACGCGATCATGTACGGCGGCATGGACGCCACCGGCGGCCCGTTCGCCAAGCAAGCCAAGCA
GCTTGGCCTGCGCGCGAAGGTGCTGGCAGGCGACGGCGTGTGTACCGACAAGCTGTCGGACCTGGCCGGCGACGCAACCG
ACAACATCGTCTGCTCGGAAGCCGGTATGGCGCTCGAGAAGATGGCCGGCGGCGCAGCGTTCCTCGCCAAGTATCAGAAG
CGTTTCGGTCAGCCGATCCAGATCTACGCTCCGTTCACGTATGACGCTGTGTACATCATCGTCGACGCTATGAAGCGTGC
TAACTCGACCGACCCGGCCAAGATCCTGGCAGCCATGCCGAGCACGGACTACAAGGGTGTGATCGGTGAAACCACGTTCG
ACTCGAAGGGCGACCTTCAGCACGGCGTGATCTCGCTGTACAACTACAAGGCAGGCAAGAAGACGCTGCTCGACGTAGTG
AAGATGTAA

Upstream 100 bases:

>100_bases
CAAGTACGGTTTGCCGCCTGCCACAAACAGTCCGGCGAGCGTGCTGGCCAAGTGCATGAACGATGCAACCGGCGTGGTCG
TCCACGGGACTGAGCGACAC

Downstream 100 bases:

>100_bases
AAATCGCGTGACCGTGGGCAGCGTTGGTCTGTCTTCGGTCGCCTGGTCAGGAAGGCACGCGCGCCGCGAGGTTCGCGTGC
CTTTTTCTTTGCCGATTCGT

Product: branched chain amino acid ABC transporter periplasmic ligand-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLGKRGHRAITLFLGPKLETYMNIKIQKLLPISAAAMLFATLATSAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVE
EINAKGLTIGGQKITLQLDAQDDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQ
QGFKTTYRVVATDAQQGPALANYAAKGLKVKSVAIVDDSTAYGQGLANEFEKTAKSLGLNVMSHDATNDKAVDFRAILTK
IKGENPDAIMYGGMDATGGPFAKQAKQLGLRAKVLAGDGVCTDKLSDLAGDATDNIVCSEAGMALEKMAGGAAFLAKYQK
RFGQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKILAAMPSTDYKGVIGETTFDSKGDLQHGVISLYNYKAGKKTLLDVV
KM

Sequences:

>Translated_402_residues
MLGKRGHRAITLFLGPKLETYMNIKIQKLLPISAAAMLFATLATSAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVE
EINAKGLTIGGQKITLQLDAQDDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQ
QGFKTTYRVVATDAQQGPALANYAAKGLKVKSVAIVDDSTAYGQGLANEFEKTAKSLGLNVMSHDATNDKAVDFRAILTK
IKGENPDAIMYGGMDATGGPFAKQAKQLGLRAKVLAGDGVCTDKLSDLAGDATDNIVCSEAGMALEKMAGGAAFLAKYQK
RFGQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKILAAMPSTDYKGVIGETTFDSKGDLQHGVISLYNYKAGKKTLLDVV
KM
>Mature_402_residues
MLGKRGHRAITLFLGPKLETYMNIKIQKLLPISAAAMLFATLATSAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVE
EINAKGLTIGGQKITLQLDAQDDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQ
QGFKTTYRVVATDAQQGPALANYAAKGLKVKSVAIVDDSTAYGQGLANEFEKTAKSLGLNVMSHDATNDKAVDFRAILTK
IKGENPDAIMYGGMDATGGPFAKQAKQLGLRAKVLAGDGVCTDKLSDLAGDATDNIVCSEAGMALEKMAGGAAFLAKYQK
RFGQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKILAAMPSTDYKGVIGETTFDSKGDLQHGVISLYNYKAGKKTLLDVV
KM

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=358, Percent_Identity=34.0782122905028, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI48994941, Length=370, Percent_Identity=31.8918918918919, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 42272; Mature: 42272

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGKRGHRAITLFLGPKLETYMNIKIQKLLPISAAAMLFATLATSAAADQVVKIGHVAPL
CCCCCCCCEEEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TGGIAHLGKDNENGARLAVEEINAKGLTIGGQKITLQLDAQDDAADPRTATQVAQKLVDD
CCCHHHCCCCCCCCCEEEEEECCCCCEEECCEEEEEEEECCCCCCCCHHHHHHHHHHHCC
KVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPAL
CEEEEEEECCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHCCCEEEEEEEECCCCCCHH
ANYAAKGLKVKSVAIVDDSTAYGQGLANEFEKTAKSLGLNVMSHDATNDKAVDFRAILTK
HHHHHCCEEEEEEEEEECCCHHCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHH
IKGENPDAIMYGGMDATGGPFAKQAKQLGLRAKVLAGDGVCTDKLSDLAGDATDNIVCSE
HCCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCCEEECC
AGMALEKMAGGAAFLAKYQKRFGQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKILAAMP
CCHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHEEECC
STDYKGVIGETTFDSKGDLQHGVISLYNYKAGKKTLLDVVKM
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MLGKRGHRAITLFLGPKLETYMNIKIQKLLPISAAAMLFATLATSAAADQVVKIGHVAPL
CCCCCCCCEEEEEECCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
TGGIAHLGKDNENGARLAVEEINAKGLTIGGQKITLQLDAQDDAADPRTATQVAQKLVDD
CCCHHHCCCCCCCCCEEEEEECCCCCEEECCEEEEEEEECCCCCCCCHHHHHHHHHHHCC
KVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPAL
CEEEEEEECCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHCCCEEEEEEEECCCCCCHH
ANYAAKGLKVKSVAIVDDSTAYGQGLANEFEKTAKSLGLNVMSHDATNDKAVDFRAILTK
HHHHHCCEEEEEEEEEECCCHHCCHHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHH
IKGENPDAIMYGGMDATGGPFAKQAKQLGLRAKVLAGDGVCTDKLSDLAGDATDNIVCSE
HCCCCCCEEEECCCCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCCCCCEEECC
AGMALEKMAGGAAFLAKYQKRFGQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKILAAMP
CCHHHHHHCCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHEEECC
STDYKGVIGETTFDSKGDLQHGVISLYNYKAGKKTLLDVVKM
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]