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The map label for this gene is oprM [H]

Identifier: 91781482

GI number: 91781482

Start: 115383

End: 116912

Strand: Reverse

Name: oprM [H]

Synonym: Bxe_A4364

Alternate gene names: 91781482

Gene position: 116912-115383 (Counterclockwise)

Preceding gene: 91781483

Following gene: 91781481

Centisome position: 2.39

GC content: 68.95

Gene sequence:

>1530_bases
ATGAACGCGCCGGCATCCCGTCGCCGTTTCGTCGTGCGGCCGCTGCGGCGCGTCTCGCTGCATGCGTGCGCCTTGACACT
CGCAAGCGCGTGCGCACTCACCGCGTGCACCGTCGGCCCCGACTACGTGAAGCCGACCGCGACCACCGCCGCCACCTACA
AGGAACTCGACGGCACCGGCTGGAAACCCGCGCAACCCGCCGACACTTTCGCACGCGGCGCTTGGTGGAGCGTCTACGCG
GATCCGTCGCTCGACGCGCTCGAACAGCAGGTCGCGAGCGCGAACCAGAACGTGCAGGCTGCCGAAGCACGCTTTCGCGC
CGCCCGTGCCACCGTCGCGCAATATCGCTCGAGTTTCTTCCCGCTGGTCAGCGCGACCGGCGCCTATTCGCGCGCGCGTT
CATCGGAGAACGTGCTGCACAAATCGACCGCGGGTCTCACGCTGAATGACTACCTCGTGCAAGCCGACGCGTCGTGGGAA
CCCGACCTGTGGGGCCGCGTGTCGCGCAGTGTCGAAGGCGCGAAAGCCGAAGCGCAGGCAAGCGCCGCCGACACACAAGC
CGCGCTGCTGTCGATGCAGGCCGAACTCGCCACCGACTACTTCGAGCTGCGCGGACTCGATCAGGAGCGCCAACTGCTCG
ACGACACGATCGAGGCCTATAGGCAAGCGCTGGAGCTGACGCAGCACCGCTATACCGGCGGCATCGCCACGGACGCCGAT
GTCGCGCAGGCGCAGACACAACTGAAAACCACCCAGGCCCAAGCGCTCGACCTCGGCGTGCAGCGCGCGCAGCTCGAACA
CGCGATCGCGATCCTGATCGGCCAACCGCCTTCCACGTTTGCATTGCCGGTCGCGCCGCTCGCGGCGGTGCCGGTCGTCG
CGGCGGCGGGCGTGCCGTCCGCATTGCTCGAACGGCGCCCCGACATCGCGGCGGCGGAACGGCGCGTCGCCGGGATGAAC
GCGCAGATCGGCGTGGCGACGGCGGCGTTTTTCCCGAACCTCGTTTTGTCCGTGACGGGCGGCCTCGAAGCGACCAACTA
CAGCCAATGGCTGCTGGCGCCGAGCCGGCTGTGGTCGCTCGGGCCGACGTTGGCCGGAACGCTGCTCGACTTCGGCGGAC
GCGCCTCCGTCAAGGAACAGGCCCGCGCGCACTACGACGAAAGCGTCGCGCAATACCGGCAGACCGTGCTGACCGCCTTC
GGCCAGGTCGAGGACAATCTCGCCGCGCTGCGCGTGCTCGAACAGGAAGCGAGCGCGCAGGACGACGCGGTGACAGCCGC
GCAACGTGCGCTGGCGGTCGTATCGGACCGCTACAGGAACGGCGCGATCACCTATCTCGACGTGGTGGTCGCGCAAACCA
CCGCGCTCACCAATGAACGCGATGCCGTATCGATCGCGCGCCGGCGCATGGCCGCGAGCGTGGCGCTGATCAAGGCGCTG
GGCGGCGGCTGGGACGAGTCCGCGCTGCCGACGGACGAACAGATCGTGCATCCGTCCCCACCCGCGAGCGATGCAGCCGC
GCACGGGTAG

Upstream 100 bases:

>100_bases
CAGCCGCGGGCAATGATCGGGCGGCGGGCGGCTCCAACGCTGTTGCGGCAGACACGCCCACCACGCATGTCGCCGCGTCG
CTGTCCGTAAAGGCATCGTC

Downstream 100 bases:

>100_bases
CCGGGGTTTGTCGATGCGGTGAAAGGGGGCCGGGCACGGCGCCTGGGTGGGTCGTGGGTGGGTCGTGGGTGGGTCGTGGG
TGGGTCGTGGGTGGGTCGTG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNAPASRRRFVVRPLRRVSLHACALTLASACALTACTVGPDYVKPTATTAATYKELDGTGWKPAQPADTFARGAWWSVYA
DPSLDALEQQVASANQNVQAAEARFRAARATVAQYRSSFFPLVSATGAYSRARSSENVLHKSTAGLTLNDYLVQADASWE
PDLWGRVSRSVEGAKAEAQASAADTQAALLSMQAELATDYFELRGLDQERQLLDDTIEAYRQALELTQHRYTGGIATDAD
VAQAQTQLKTTQAQALDLGVQRAQLEHAIAILIGQPPSTFALPVAPLAAVPVVAAAGVPSALLERRPDIAAAERRVAGMN
AQIGVATAAFFPNLVLSVTGGLEATNYSQWLLAPSRLWSLGPTLAGTLLDFGGRASVKEQARAHYDESVAQYRQTVLTAF
GQVEDNLAALRVLEQEASAQDDAVTAAQRALAVVSDRYRNGAITYLDVVVAQTTALTNERDAVSIARRRMAASVALIKAL
GGGWDESALPTDEQIVHPSPPASDAAAHG

Sequences:

>Translated_509_residues
MNAPASRRRFVVRPLRRVSLHACALTLASACALTACTVGPDYVKPTATTAATYKELDGTGWKPAQPADTFARGAWWSVYA
DPSLDALEQQVASANQNVQAAEARFRAARATVAQYRSSFFPLVSATGAYSRARSSENVLHKSTAGLTLNDYLVQADASWE
PDLWGRVSRSVEGAKAEAQASAADTQAALLSMQAELATDYFELRGLDQERQLLDDTIEAYRQALELTQHRYTGGIATDAD
VAQAQTQLKTTQAQALDLGVQRAQLEHAIAILIGQPPSTFALPVAPLAAVPVVAAAGVPSALLERRPDIAAAERRVAGMN
AQIGVATAAFFPNLVLSVTGGLEATNYSQWLLAPSRLWSLGPTLAGTLLDFGGRASVKEQARAHYDESVAQYRQTVLTAF
GQVEDNLAALRVLEQEASAQDDAVTAAQRALAVVSDRYRNGAITYLDVVVAQTTALTNERDAVSIARRRMAASVALIKAL
GGGWDESALPTDEQIVHPSPPASDAAAHG
>Mature_509_residues
MNAPASRRRFVVRPLRRVSLHACALTLASACALTACTVGPDYVKPTATTAATYKELDGTGWKPAQPADTFARGAWWSVYA
DPSLDALEQQVASANQNVQAAEARFRAARATVAQYRSSFFPLVSATGAYSRARSSENVLHKSTAGLTLNDYLVQADASWE
PDLWGRVSRSVEGAKAEAQASAADTQAALLSMQAELATDYFELRGLDQERQLLDDTIEAYRQALELTQHRYTGGIATDAD
VAQAQTQLKTTQAQALDLGVQRAQLEHAIAILIGQPPSTFALPVAPLAAVPVVAAAGVPSALLERRPDIAAAERRVAGMN
AQIGVATAAFFPNLVLSVTGGLEATNYSQWLLAPSRLWSLGPTLAGTLLDFGGRASVKEQARAHYDESVAQYRQTVLTAF
GQVEDNLAALRVLEQEASAQDDAVTAAQRALAVVSDRYRNGAITYLDVVVAQTTALTNERDAVSIARRRMAASVALIKAL
GGGWDESALPTDEQIVHPSPPASDAAAHG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=25.3763440860215, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI2367348, Length=494, Percent_Identity=26.9230769230769, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54106; Mature: 54106

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPASRRRFVVRPLRRVSLHACALTLASACALTACTVGPDYVKPTATTAATYKELDGTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
WKPAQPADTFARGAWWSVYADPSLDALEQQVASANQNVQAAEARFRAARATVAQYRSSFF
CCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
PLVSATGAYSRARSSENVLHKSTAGLTLNDYLVQADASWEPDLWGRVSRSVEGAKAEAQA
HHHHCCCHHHHHCCHHHHHHHHCCCCEEEHEEEEECCCCCCHHHHHHHHHCCCCHHHHHH
SAADTQAALLSMQAELATDYFELRGLDQERQLLDDTIEAYRQALELTQHRYTGGIATDAD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
VAQAQTQLKTTQAQALDLGVQRAQLEHAIAILIGQPPSTFALPVAPLAAVPVVAAAGVPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCH
ALLERRPDIAAAERRVAGMNAQIGVATAAFFPNLVLSVTGGLEATNYSQWLLAPSRLWSL
HHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHC
GPTLAGTLLDFGGRASVKEQARAHYDESVAQYRQTVLTAFGQVEDNLAALRVLEQEASAQ
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDAVTAAQRALAVVSDRYRNGAITYLDVVVAQTTALTNERDAVSIARRRMAASVALIKAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GGGWDESALPTDEQIVHPSPPASDAAAHG
CCCCCCCCCCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MNAPASRRRFVVRPLRRVSLHACALTLASACALTACTVGPDYVKPTATTAATYKELDGTG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
WKPAQPADTFARGAWWSVYADPSLDALEQQVASANQNVQAAEARFRAARATVAQYRSSFF
CCCCCCHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
PLVSATGAYSRARSSENVLHKSTAGLTLNDYLVQADASWEPDLWGRVSRSVEGAKAEAQA
HHHHCCCHHHHHCCHHHHHHHHCCCCEEEHEEEEECCCCCCHHHHHHHHHCCCCHHHHHH
SAADTQAALLSMQAELATDYFELRGLDQERQLLDDTIEAYRQALELTQHRYTGGIATDAD
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
VAQAQTQLKTTQAQALDLGVQRAQLEHAIAILIGQPPSTFALPVAPLAAVPVVAAAGVPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCH
ALLERRPDIAAAERRVAGMNAQIGVATAAFFPNLVLSVTGGLEATNYSQWLLAPSRLWSL
HHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHC
GPTLAGTLLDFGGRASVKEQARAHYDESVAQYRQTVLTAFGQVEDNLAALRVLEQEASAQ
CHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DDAVTAAQRALAVVSDRYRNGAITYLDVVVAQTTALTNERDAVSIARRRMAASVALIKAL
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GGGWDESALPTDEQIVHPSPPASDAAAHG
CCCCCCCCCCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]