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The map label for this gene is htrB [H]

Identifier: 91781474

GI number: 91781474

Start: 107696

End: 108580

Strand: Reverse

Name: htrB [H]

Synonym: Bxe_A4372

Alternate gene names: 91781474

Gene position: 108580-107696 (Counterclockwise)

Preceding gene: 91781476

Following gene: 91781473

Centisome position: 2.22

GC content: 63.84

Gene sequence:

>885_bases
ATGCTGAGCCGCTATGGCGCAAAACTCGCGATCGGGCTGTTGAAACTGTTTGCACTATTGCCTTACGGCCTGATCGCCCG
CTTCGGCGACGGCCTCGGCTGGCTGCTGTATCAGATCCCGAGCCGGCGCAAACGCATCGTCCACATCAATCTGAAGCTGT
GCTTCCCCGAATGGAGCGACGAACGCCGCGAAGACGTCGCGCAGAAGCATTTCCGCCACGCGATCCGCAGCTACCTCGAA
CGCAGCGTGCAGTGGTTCGGCTCGGCGAAGAAGCTCGAAAAATTGATCCAGCTCGACAGCGCGATCGATCTGACCGACCC
GAATCTGCCGCCCACGCTGTTTCTCGGCTTTCACTTCGTCGGTATCGAGGCCGGCTCGATCTTCCTGAACTATTCGCTCA
GGCGCCAATGCGGCTCGCTCTATCAGCCGATGTCGAATCCGGAGCTCGAGGCAGTCGCCAAAGCGGCGCGCGCGCGTTTC
GGTGCGGACATGGCGAGCCGCGCCGACAGCGCGCGCATCGTGCTGCGCTGGCTGCGCGAGCGCAAACCGGTCATGCTCGG
CGCCGATATGGACTACGGCGCGCGCAACTCCACCTTCGTGCCGTTCTTCGGCGTGCCGACCTGCACGCTGACCGCGGTCG
GGCGTCTGGCCAAGGTCGGGCACGCGCAGGTGGTGCCGTTCATCGGCGAGGTGCTGCCGAACTACAAAGGCTACCGGCTC
AAGGTGTTCAAGCCGTGGGAGAACTACCCGACCGGCGACGACGACGCGGACGCCCGCCGCATGAACGCCTTTCTCGAAGA
GCAGATTCCGTTGATGCCCGAGCAGTACTACTGGGTGCACAAGCGCTTCAAGACCCGGCCGCCCGGCGACCCGAGCGTGT
ACTGA

Upstream 100 bases:

>100_bases
TTCCTCAGATTGTCACAGAGTGTCCACGAGTGCGGTATTACGTCAACTTCTCCGCTTTCCTGAAGCCCGTCACGCGCAAC
GCCTCTTTCCAGGAGGCGGC

Downstream 100 bases:

>100_bases
ACTGAACGGCCGCCCACGCGGCCCGTGTGGAAAGCGACCGGACGCGGAAAAAATACGGCGTTCGGCTCACCTGCGGGCGC
GTCACTTACAATAGCGTGAT

Product: lipid A biosynthesis lauroyl acyltransferase

Products: KDO2-(lauroyl)-lipid IVA [C]

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MLSRYGAKLAIGLLKLFALLPYGLIARFGDGLGWLLYQIPSRRKRIVHINLKLCFPEWSDERREDVAQKHFRHAIRSYLE
RSVQWFGSAKKLEKLIQLDSAIDLTDPNLPPTLFLGFHFVGIEAGSIFLNYSLRRQCGSLYQPMSNPELEAVAKAARARF
GADMASRADSARIVLRWLRERKPVMLGADMDYGARNSTFVPFFGVPTCTLTAVGRLAKVGHAQVVPFIGEVLPNYKGYRL
KVFKPWENYPTGDDDADARRMNAFLEEQIPLMPEQYYWVHKRFKTRPPGDPSVY

Sequences:

>Translated_294_residues
MLSRYGAKLAIGLLKLFALLPYGLIARFGDGLGWLLYQIPSRRKRIVHINLKLCFPEWSDERREDVAQKHFRHAIRSYLE
RSVQWFGSAKKLEKLIQLDSAIDLTDPNLPPTLFLGFHFVGIEAGSIFLNYSLRRQCGSLYQPMSNPELEAVAKAARARF
GADMASRADSARIVLRWLRERKPVMLGADMDYGARNSTFVPFFGVPTCTLTAVGRLAKVGHAQVVPFIGEVLPNYKGYRL
KVFKPWENYPTGDDDADARRMNAFLEEQIPLMPEQYYWVHKRFKTRPPGDPSVY
>Mature_294_residues
MLSRYGAKLAIGLLKLFALLPYGLIARFGDGLGWLLYQIPSRRKRIVHINLKLCFPEWSDERREDVAQKHFRHAIRSYLE
RSVQWFGSAKKLEKLIQLDSAIDLTDPNLPPTLFLGFHFVGIEAGSIFLNYSLRRQCGSLYQPMSNPELEAVAKAARARF
GADMASRADSARIVLRWLRERKPVMLGADMDYGARNSTFVPFFGVPTCTLTAVGRLAKVGHAQVVPFIGEVLPNYKGYRL
KVFKPWENYPTGDDDADARRMNAFLEEQIPLMPEQYYWVHKRFKTRPPGDPSVY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=289, Percent_Identity=34.6020761245675, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI87082094, Length=289, Percent_Identity=30.7958477508651, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788161, Length=300, Percent_Identity=30.6666666666667, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.- [C]

Molecular weight: Translated: 33628; Mature: 33628

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRYGAKLAIGLLKLFALLPYGLIARFGDGLGWLLYQIPSRRKRIVHINLKLCFPEWSD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEEEEECCCCCH
ERREDVAQKHFRHAIRSYLERSVQWFGSAKKLEKLIQLDSAIDLTDPNLPPTLFLGFHFV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEE
GIEAGSIFLNYSLRRQCGSLYQPMSNPELEAVAKAARARFGADMASRADSARIVLRWLRE
EEEECEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHH
RKPVMLGADMDYGARNSTFVPFFGVPTCTLTAVGRLAKVGHAQVVPFIGEVLPNYKGYRL
CCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEE
KVFKPWENYPTGDDDADARRMNAFLEEQIPLMPEQYYWVHKRFKTRPPGDPSVY
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLSRYGAKLAIGLLKLFALLPYGLIARFGDGLGWLLYQIPSRRKRIVHINLKLCFPEWSD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEEEEEEECCCCCH
ERREDVAQKHFRHAIRSYLERSVQWFGSAKKLEKLIQLDSAIDLTDPNLPPTLFLGFHFV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEE
GIEAGSIFLNYSLRRQCGSLYQPMSNPELEAVAKAARARFGADMASRADSARIVLRWLRE
EEEECEEEEEHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHH
RKPVMLGADMDYGARNSTFVPFFGVPTCTLTAVGRLAKVGHAQVVPFIGEVLPNYKGYRL
CCCEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEE
KVFKPWENYPTGDDDADARRMNAFLEEQIPLMPEQYYWVHKRFKTRPPGDPSVY
EEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO2-lipid IVA; lauroyl-ACP [C]

Specific reaction: KDO2-lipid IVA + lauroyl-ACP = KDO2-(lauroyl)-lipid IVA + [acyl carrier protein] [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]