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The map label for this gene is exoA [H]

Identifier: 91781453

GI number: 91781453

Start: 86576

End: 87355

Strand: Direct

Name: exoA [H]

Synonym: Bxe_A4393

Alternate gene names: 91781453

Gene position: 86576-87355 (Clockwise)

Preceding gene: 91781452

Following gene: 91781455

Centisome position: 1.77

GC content: 58.59

Gene sequence:

>780_bases
ATGTTGCGTGTGATTACCGCCAACCTGAACGGCATCCGCTCAGCGGCGAAGAAGGGCTTTTTCGACTGGTTCGGAGAACA
GAAGGCCGACGTGCTGTGCGTGCAAGAAATCAAATGCTCGCAAGACGACATGACCCCGGAATTCATGGCGCCGCACGATT
TCACCGGCTATTTTCAGCACGCCGTGAAGAAAGGCTATAGCGGCGCGGGCGTGTACACCCGTCACGAACCGGACGAAGTG
GTGATCGGCTTCGGCAGCGAGGAATTCGATCCGGAAGGGCGCTATGTCGAACTGCGTTTCGGCAAGCTGTCGGTGATTTC
GGTGTACGTGCCGTCGGGTTCGAGCGGCGACGAGCGCCAGCAGGCCAAATACCGTTTCATGGACGAGTTCATGCCGCATC
TCGCGGAACTGGCTCAGGAGCGCGAAGTGATCGTGTGCGGCGACGTCAACATCGTCCACAAGGAAATCGACATCAAGAAC
TGGAAGAGCAACCAGAAGAATTCGGGTTGCCTGCCGGAAGAGCGCGCGTGGCTCACCAAACTGTTCGATGAGGTCGGTTA
CGTCGACGTGTTCCGCACGCTCGACCAGCGGCCGGAACAGTACACGTGGTGGAGCAATCGCGGCCAGGCGTATGCGAAGA
ACGTCGGGTGGCGGATCGATTATCAGATCGCCACGCCGGGTATCGCGAGCAAGGCGAAACGCACCGACGTGTTCCGCGAT
ATCAAGTTCAGCGACCATGCGCCGCTGACGGTCGACTACGACCACAAGCTCAAGAAGTAA

Upstream 100 bases:

>100_bases
AAGCTCGAATATCCACCGGCCAAAGAATCGCTGAAGGGCATCAAGATCGTCTGACCGCTGCCCTTTGCTTCAGAAACGAA
TCGACCATACAAGGAACGAC

Downstream 100 bases:

>100_bases
GCGGAGTTCGCGTCCTCGCGGCAGAAGAGGCTGATGTCGGCCTCAGGGGGACGCTTCGTCAGCGCAGAGCCGCGGGTACG
TTCTTCGGCCCGCGCAACTC

Product: exodeoxyribonucleaseiii xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MLRVITANLNGIRSAAKKGFFDWFGEQKADVLCVQEIKCSQDDMTPEFMAPHDFTGYFQHAVKKGYSGAGVYTRHEPDEV
VIGFGSEEFDPEGRYVELRFGKLSVISVYVPSGSSGDERQQAKYRFMDEFMPHLAELAQEREVIVCGDVNIVHKEIDIKN
WKSNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIASKAKRTDVFRD
IKFSDHAPLTVDYDHKLKK

Sequences:

>Translated_259_residues
MLRVITANLNGIRSAAKKGFFDWFGEQKADVLCVQEIKCSQDDMTPEFMAPHDFTGYFQHAVKKGYSGAGVYTRHEPDEV
VIGFGSEEFDPEGRYVELRFGKLSVISVYVPSGSSGDERQQAKYRFMDEFMPHLAELAQEREVIVCGDVNIVHKEIDIKN
WKSNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIASKAKRTDVFRD
IKFSDHAPLTVDYDHKLKK
>Mature_259_residues
MLRVITANLNGIRSAAKKGFFDWFGEQKADVLCVQEIKCSQDDMTPEFMAPHDFTGYFQHAVKKGYSGAGVYTRHEPDEV
VIGFGSEEFDPEGRYVELRFGKLSVISVYVPSGSSGDERQQAKYRFMDEFMPHLAELAQEREVIVCGDVNIVHKEIDIKN
WKSNQKNSGCLPEERAWLTKLFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIASKAKRTDVFRD
IKFSDHAPLTVDYDHKLKK

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=253, Percent_Identity=34.7826086956522, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI18375503, Length=253, Percent_Identity=34.7826086956522, Blast_Score=171, Evalue=6e-43,
Organism=Homo sapiens, GI18375501, Length=253, Percent_Identity=34.7826086956522, Blast_Score=171, Evalue=6e-43,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=29.3233082706767, Blast_Score=94, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=30.7392996108949, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=32.8063241106719, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=32.8063241106719, Blast_Score=147, Evalue=8e-36,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29796; Mature: 29796

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRVITANLNGIRSAAKKGFFDWFGEQKADVLCVQEIKCSQDDMTPEFMAPHDFTGYFQH
CEEEEECCCHHHHHHHHHCHHHHHCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCHHHHHH
AVKKGYSGAGVYTRHEPDEVVIGFGSEEFDPEGRYVELRFGKLSVISVYVPSGSSGDERQ
HHHCCCCCCCCEECCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEEECCCCCCCHHH
QAKYRFMDEFMPHLAELAQEREVIVCGDVNIVHKEIDIKNWKSNQKNSGCLPEERAWLTK
HHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIASKAKRTDVFRD
HHHHCCHHHHHHHHHCCCCCCEEECCCCCEEHHCCCEEEEEEEECCCCHHHHHHHHHHHC
IKFSDHAPLTVDYDHKLKK
CCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MLRVITANLNGIRSAAKKGFFDWFGEQKADVLCVQEIKCSQDDMTPEFMAPHDFTGYFQH
CEEEEECCCHHHHHHHHHCHHHHHCCCCCCEEEEHHHCCCCCCCCCCCCCCCCCHHHHHH
AVKKGYSGAGVYTRHEPDEVVIGFGSEEFDPEGRYVELRFGKLSVISVYVPSGSSGDERQ
HHHCCCCCCCCEECCCCCEEEEECCCCCCCCCCCEEEEEECCEEEEEEEECCCCCCCHHH
QAKYRFMDEFMPHLAELAQEREVIVCGDVNIVHKEIDIKNWKSNQKNSGCLPEERAWLTK
HHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LFDEVGYVDVFRTLDQRPEQYTWWSNRGQAYAKNVGWRIDYQIATPGIASKAKRTDVFRD
HHHHCCHHHHHHHHHCCCCCCEEECCCCCEEHHCCCEEEEEEEECCCCHHHHHHHHHHHC
IKFSDHAPLTVDYDHKLKK
CCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]