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The map label for this gene is gatB [H]

Identifier: 91781451

GI number: 91781451

Start: 84079

End: 85554

Strand: Direct

Name: gatB [H]

Synonym: Bxe_A4395

Alternate gene names: 91781451

Gene position: 84079-85554 (Clockwise)

Preceding gene: 91781450

Following gene: 91781452

Centisome position: 1.72

GC content: 61.99

Gene sequence:

>1476_bases
ATGACCAAGCAATGGGAAGTCGTGATCGGTCTGGAGACCCACGCGCAACTGTCGACCCACTCGAAGATTTTTTCGGGCGC
CTCGACGCAATTCGGCGCTGCGCCGAATACGCAGGCCTGCCCCGTCGATCTGGCGTTGCCGGGCACGTTGCCGGTGATGA
ACCGCGGCGCCGTGGAGCGCGCAATCCAGTTCGGCCTCGCGATCGGCGCGACAGTGGCGCCGCGCAGCATCTTCGCGCGC
AAGAATTATTTCTACCCCGATCTGCCCAAGGGCTACCAGATCAGCCAGTACGAAATCCCCGTCGTGCAGGGCGGCCAGGT
GACGATTCAGGTGCCGGCCAATGAAAAGGCGAATACTCCGGCGTACGAAAAGGTCGTCAACCTCACGCGCGCCCACCTCG
AAGAAGACGCGGGCAAGTCGCTGCACGAAGACTTCGCGGGCATGACCGGCATCGACCTGAATCGCGCCGGCACGCCGCTG
CTCGAAATCGTCACCGAGCCGGAAATGCGCAGCGCGGCGGAAGCGGTCGCCTACGCGAAGACGCTGCATACGCTCGTCAC
GTGGCTCGGTATCTGCGACGGCAACATGCAGGAAGGCTCGTTCCGCTGCGACGCGAATGTATCGGTGCGTCCGGTCGGTC
AGGCGGAATTCGGCACGCGTGCCGAGATCAAGAACCTGAACTCGTTCCGCTTCCTCGAAGAAGCGATCCAGTACGAAGTG
CGCCGTCAGATCGAACTGATCGAAGACGGCGGCACGGTGGTGCAGGAAACGCGTCTGTACGATCCGGACAAGCGCGAAAC
GCGCTCCATGCGCAGCAAGGAAGACGCACACGATTACCGCTATTTCCCCGATCCCGATCTGATGCCGCTCGTGATCGACG
CGGCGTGGGTCGAGCGCGTCAAAAGCGAAATGCCGGAACTGCCGGTTGCGATTCAGCAGCGCTTCGTCACGCAATACGGT
TTGACGCCGTACGACGCCAACGTGCTGACGTCGAGCAAGGCAATGGCGGCGTACTACGAAGCGGTGGTGAGCAAGCTCGG
CCCGGCCAACGCCAAGGCCGCGGCGAACTGGCTGATGGGCGAAGTGTCGTCGCAACTGAATCGCGAGGACCTCGACATCG
CTGCGAGCCCGGTATCGTCCGCGCAACTTGCGCTGCTGCTACAGCGTATCGCGGACGGCACGATCTCCCACAAGATCGCC
AAGGAAATTTTCCTGTCGATCTGGGAAGAGAAAGCCACCGACGAAGCCGCCACCGATCGCATCATCGAAGCGAAAGGCCT
GAAGCAGATTTCGGATACCGGCGCGCTGGAAGCGATCATCGACGAAGTGCTCGCCGCGAATCAGAAGTCGGTCGAGGAAT
TCCGCGCCGGCAAGGAAAAGGCGTTCAACGCGCTGATCGGCCAGGCCATGAAGGCGACCAAGGGCAAGGCCAATCCGGCG
CAGGTGAACGAACTGCTCAGGAAGAAGCTGTCCTGA

Upstream 100 bases:

>100_bases
TCGGCAACTATTTCAATGAAGCCCGGATGCTGCAGGTGGCCGACGCGTTCCAGCGCGCGACCGACTGGCACCGCAAAGCA
CCGGCAGGAGTGTGAGCACC

Downstream 100 bases:

>100_bases
ACTGAAGCAGGGTTGAAAAAAGTACGGCCGAAGCGTTCGCGCTTTGGCCACCGCGGGCTGTTGCCGGAAACGAAAGTGGG
GCAGCGGCCCGTTTCGTTTG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTKQWEVVIGLETHAQLSTHSKIFSGASTQFGAAPNTQACPVDLALPGTLPVMNRGAVERAIQFGLAIGATVAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGQVTIQVPANEKANTPAYEKVVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKTLHTLVTWLGICDGNMQEGSFRCDANVSVRPVGQAEFGTRAEIKNLNSFRFLEEAIQYEV
RRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDAAWVERVKSEMPELPVAIQQRFVTQYG
LTPYDANVLTSSKAMAAYYEAVVSKLGPANAKAAANWLMGEVSSQLNREDLDIAASPVSSAQLALLLQRIADGTISHKIA
KEIFLSIWEEKATDEAATDRIIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPA
QVNELLRKKLS

Sequences:

>Translated_491_residues
MTKQWEVVIGLETHAQLSTHSKIFSGASTQFGAAPNTQACPVDLALPGTLPVMNRGAVERAIQFGLAIGATVAPRSIFAR
KNYFYPDLPKGYQISQYEIPVVQGGQVTIQVPANEKANTPAYEKVVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPL
LEIVTEPEMRSAAEAVAYAKTLHTLVTWLGICDGNMQEGSFRCDANVSVRPVGQAEFGTRAEIKNLNSFRFLEEAIQYEV
RRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDAAWVERVKSEMPELPVAIQQRFVTQYG
LTPYDANVLTSSKAMAAYYEAVVSKLGPANAKAAANWLMGEVSSQLNREDLDIAASPVSSAQLALLLQRIADGTISHKIA
KEIFLSIWEEKATDEAATDRIIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPA
QVNELLRKKLS
>Mature_490_residues
TKQWEVVIGLETHAQLSTHSKIFSGASTQFGAAPNTQACPVDLALPGTLPVMNRGAVERAIQFGLAIGATVAPRSIFARK
NYFYPDLPKGYQISQYEIPVVQGGQVTIQVPANEKANTPAYEKVVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLL
EIVTEPEMRSAAEAVAYAKTLHTLVTWLGICDGNMQEGSFRCDANVSVRPVGQAEFGTRAEIKNLNSFRFLEEAIQYEVR
RQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDAAWVERVKSEMPELPVAIQQRFVTQYGL
TPYDANVLTSSKAMAAYYEAVVSKLGPANAKAAANWLMGEVSSQLNREDLDIAASPVSSAQLALLLQRIADGTISHKIAK
EIFLSIWEEKATDEAATDRIIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPAQ
VNELLRKKLS

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=501, Percent_Identity=38.5229540918164, Blast_Score=352, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI115534094, Length=466, Percent_Identity=33.0472103004292, Blast_Score=229, Evalue=3e-60,
Organism=Saccharomyces cerevisiae, GI6319391, Length=523, Percent_Identity=31.5487571701721, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45550808, Length=498, Percent_Identity=35.140562248996, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53857; Mature: 53726

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKQWEVVIGLETHAQLSTHSKIFSGASTQFGAAPNTQACPVDLALPGTLPVMNRGAVER
CCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHH
AIQFGLAIGATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQVTIQVPANEKANTP
HHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCCCCH
AYEKVVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
TLHTLVTWLGICDGNMQEGSFRCDANVSVRPVGQAEFGTRAEIKNLNSFRFLEEAIQYEV
HHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHH
RRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDAAWVERV
HHHHHHHCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KSEMPELPVAIQQRFVTQYGLTPYDANVLTSSKAMAAYYEAVVSKLGPANAKAAANWLMG
HHHCCCCCHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EVSSQLNREDLDIAASPVSSAQLALLLQRIADGTISHKIAKEIFLSIWEEKATDEAATDR
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
IIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPA
HHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLRKKLS
HHHHHHHHHCC
>Mature Secondary Structure 
TKQWEVVIGLETHAQLSTHSKIFSGASTQFGAAPNTQACPVDLALPGTLPVMNRGAVER
CCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHHH
AIQFGLAIGATVAPRSIFARKNYFYPDLPKGYQISQYEIPVVQGGQVTIQVPANEKANTP
HHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCCCEEECCEEECCEEEEEECCCCCCCCH
AYEKVVNLTRAHLEEDAGKSLHEDFAGMTGIDLNRAGTPLLEIVTEPEMRSAAEAVAYAK
HHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHH
TLHTLVTWLGICDGNMQEGSFRCDANVSVRPVGQAEFGTRAEIKNLNSFRFLEEAIQYEV
HHHHHHHHHHHCCCCCCCCCEEECCCCEEEECCCCCCCCHHHHHCCHHHHHHHHHHHHHH
RRQIELIEDGGTVVQETRLYDPDKRETRSMRSKEDAHDYRYFPDPDLMPLVIDAAWVERV
HHHHHHHCCCCCEEEHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHH
KSEMPELPVAIQQRFVTQYGLTPYDANVLTSSKAMAAYYEAVVSKLGPANAKAAANWLMG
HHHCCCCCHHHHHHHHHHHCCCCCCCCHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EVSSQLNREDLDIAASPVSSAQLALLLQRIADGTISHKIAKEIFLSIWEEKATDEAATDR
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHH
IIEAKGLKQISDTGALEAIIDEVLAANQKSVEEFRAGKEKAFNALIGQAMKATKGKANPA
HHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHH
QVNELLRKKLS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA