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The map label for this gene is hpcH [H]

Identifier: 91781442

GI number: 91781442

Start: 73714

End: 74496

Strand: Direct

Name: hpcH [H]

Synonym: Bxe_A4404

Alternate gene names: 91781442

Gene position: 73714-74496 (Clockwise)

Preceding gene: 91781441

Following gene: 91781443

Centisome position: 1.51

GC content: 68.2

Gene sequence:

>783_bases
ATGAGCAGCCTGACCAATCCCCTCAAGCAGCGCCTCAAGGAAACCGGGCCGCTGTTCGGCCTGTGGCTCTCGCTCGGCAG
CGATGCCGCGGCCGAAGCGCTCGCGCACGCCGGTTACGACTGGCTGCTGATCGACATGGAGCACGCGCCGAACGATAGCG
GCGACGTCGCCTCGCAATTGCGCGCGATCGCCGCCGCGCATCTGCCGAGCGAACCGGTGGTGCGCGTGCCGGCCAACGAG
GCATGGCTCGTCAAGCGCGTGCTGGATGCCGGCGCGCGTACGCTGATGTTCCCGAATATCGAGTCCGCGGACGAAGCGGC
GCGCGCCGTGCGTCTCACGCAGTATCCGACCGCCGGCGCGCTCGACGGCGAGCGCGGCGTTGCCGGCGTGGTGCGGGCCG
CGGCCTACGGCATGCGGCGCGATTACGTGCAGACCGCCAACGCGCAGATTGCGACCATCGTGCAGATCGAATCGGCGCGC
GGCTTGCAGGAAGTCGAAAAGATCGCGGCGACGCCGGGTGTCGATTGCCTGTTCGTCGGACCGGCCGACCTCGCCGCGAG
CCTCGGTCATCTCGGCGATTCGAAGCACGCCGACGTGCAGGCCGCGATGGCGCGCATCGTCAGCGCCGCGGACAAGGCGG
GCATTGCCGCCGGGATTTTTGCTATGGACGTCGCGAGCGCAAAGCAGCATCGCGACGCCGGTTTTCGTTTTATCGCTCTG
GCCGCCGACGTCATGTGGATGTTGCGCGCGACTCGCCAGGCGTTGCAGGAGGTGAGGTCATGA

Upstream 100 bases:

>100_bases
ACGGCCTGAGCCATTGTCTTTCTCTTTCTCTAGCCCGTCACGCCGGCATTGCACGTTGCGGTTCGGGCGGCGCATGTCAT
CGCCGCTGCGGAGCGCGGCG

Downstream 100 bases:

>100_bases
AGGGGATGGTGCAGTGCGTCGTGGTGCGTCTGGCGCTCACGGCGGCGTTGATGGCGAATGTGGCGGCGTATGCGCAGGAC
CGCGTCGCGAAGTCGGACGA

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: 2-Hydroxy-3-oxopropanoate; Pyruvate [C]

Alternate protein names: 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; HHED aldolase; 4-hydroxy-2-ketoheptane-1,7-dioate aldolase; HKHD aldolase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MSSLTNPLKQRLKETGPLFGLWLSLGSDAAAEALAHAGYDWLLIDMEHAPNDSGDVASQLRAIAAAHLPSEPVVRVPANE
AWLVKRVLDAGARTLMFPNIESADEAARAVRLTQYPTAGALDGERGVAGVVRAAAYGMRRDYVQTANAQIATIVQIESAR
GLQEVEKIAATPGVDCLFVGPADLAASLGHLGDSKHADVQAAMARIVSAADKAGIAAGIFAMDVASAKQHRDAGFRFIAL
AADVMWMLRATRQALQEVRS

Sequences:

>Translated_260_residues
MSSLTNPLKQRLKETGPLFGLWLSLGSDAAAEALAHAGYDWLLIDMEHAPNDSGDVASQLRAIAAAHLPSEPVVRVPANE
AWLVKRVLDAGARTLMFPNIESADEAARAVRLTQYPTAGALDGERGVAGVVRAAAYGMRRDYVQTANAQIATIVQIESAR
GLQEVEKIAATPGVDCLFVGPADLAASLGHLGDSKHADVQAAMARIVSAADKAGIAAGIFAMDVASAKQHRDAGFRFIAL
AADVMWMLRATRQALQEVRS
>Mature_259_residues
SSLTNPLKQRLKETGPLFGLWLSLGSDAAAEALAHAGYDWLLIDMEHAPNDSGDVASQLRAIAAAHLPSEPVVRVPANEA
WLVKRVLDAGARTLMFPNIESADEAARAVRLTQYPTAGALDGERGVAGVVRAAAYGMRRDYVQTANAQIATIVQIESARG
LQEVEKIAATPGVDCLFVGPADLAASLGHLGDSKHADVQAAMARIVSAADKAGIAAGIFAMDVASAKQHRDAGFRFIALA
ADVMWMLRATRQALQEVRS

Specific function: Catalyzes the reversible retro-aldol cleavage of 4- hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=252, Percent_Identity=41.2698412698413, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1788578, Length=258, Percent_Identity=39.922480620155, Blast_Score=170, Evalue=8e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR012689
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.2.20 [C]

Molecular weight: Translated: 27477; Mature: 27345

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLTNPLKQRLKETGPLFGLWLSLGSDAAAEALAHAGYDWLLIDMEHAPNDSGDVASQL
CCCCCHHHHHHHHHHCCCEEHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
RAIAAAHLPSEPVVRVPANEAWLVKRVLDAGARTLMFPNIESADEAARAVRLTQYPTAGA
HHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCC
LDGERGVAGVVRAAAYGMRRDYVQTANAQIATIVQIESARGLQEVEKIAATPGVDCLFVG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCHHHHHHHHCCCCCCEEEEC
PADLAASLGHLGDSKHADVQAAMARIVSAADKAGIAAGIFAMDVASAKQHRDAGFRFIAL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHH
AADVMWMLRATRQALQEVRS
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SSLTNPLKQRLKETGPLFGLWLSLGSDAAAEALAHAGYDWLLIDMEHAPNDSGDVASQL
CCCCHHHHHHHHHHCCCEEHHHHHCCHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHH
RAIAAAHLPSEPVVRVPANEAWLVKRVLDAGARTLMFPNIESADEAARAVRLTQYPTAGA
HHHHHHCCCCCCEEECCCCHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCC
LDGERGVAGVVRAAAYGMRRDYVQTANAQIATIVQIESARGLQEVEKIAATPGVDCLFVG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEHHHCCHHHHHHHHCCCCCCEEEEC
PADLAASLGHLGDSKHADVQAAMARIVSAADKAGIAAGIFAMDVASAKQHRDAGFRFIAL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHH
AADVMWMLRATRQALQEVRS
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 5-Dehydro-4-deoxy-D-glucarate [C]

Specific reaction: 5-Dehydro-4-deoxy-D-glucarate --> 2-Hydroxy-3-oxopropanoate + Pyruvate [C]

General reaction: Carbon-Carbon Lyases; Aldehyde-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12384590; 12704152 [H]