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The map label for this gene is def [H]

Identifier: 91781430

GI number: 91781430

Start: 62298

End: 62801

Strand: Direct

Name: def [H]

Synonym: Bxe_A4416

Alternate gene names: 91781430

Gene position: 62298-62801 (Clockwise)

Preceding gene: 91781426

Following gene: 91781431

Centisome position: 1.27

GC content: 57.94

Gene sequence:

>504_bases
ATGGCTTTACTGAACATCATCAATTACCCGGACAAACGGCTGCACAAGATTGCGAAGCCGGTCGAAGCGGTCAACGACCG
CATCCGCCGCCTCGTCAAGGATATGGCCGAGACCATGTACGCCGCGCCCGGCGTCGGCCTCGCGGCGACCCAGGTGGACG
TGCATGAGCGTGTGATCGTGATCGACGTGTCGGACGACCATAACGAATTGCTTACCTTCATCAATCCGGAAATCATCTGG
TCGAGCGACGAAAGAAAGCTGTCCGAAGAGGGGTGTCTCTCGGTGCCGGGCATTTACGACAATGTCGAGCGCGCGGAGAA
AGTGCGCGTGCGGGCGCTCAACGAGAAGGGCGAAACCTTCGAGATGGACTGTGAAGGCCTGCTTGCGGTGTGCATCCAGC
ACGAAATGGATCACCTGATGGGCCGCGTGTTCGTCGAGTATCTGTCGTCGCTCAAGCAGACGCGCATCAAGAGCAAGATG
AAGAAGCTCGCCCACGCAATGTAA

Upstream 100 bases:

>100_bases
CCATCTATCCTGTGCGCAACGCGACGAACCCACAACCTGGCCGAATGGCCAACGCGAGCCTTCCACCGGTCAGCGGCGCC
TGAAAATCACACTCGAAATC

Downstream 100 bases:

>100_bases
TGCGCGTTCACCTCGCCTACCGCCCCCGTTCATGAGTCATTCGTTGCGCGTCATCTTTGCCGGTACGCCGGAGTTCGCCG
CGGCCGCACTGGCCGCGATC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MALLNIINYPDKRLHKIAKPVEAVNDRIRRLVKDMAETMYAAPGVGLAATQVDVHERVIVIDVSDDHNELLTFINPEIIW
SSDERKLSEEGCLSVPGIYDNVERAEKVRVRALNEKGETFEMDCEGLLAVCIQHEMDHLMGRVFVEYLSSLKQTRIKSKM
KKLAHAM

Sequences:

>Translated_167_residues
MALLNIINYPDKRLHKIAKPVEAVNDRIRRLVKDMAETMYAAPGVGLAATQVDVHERVIVIDVSDDHNELLTFINPEIIW
SSDERKLSEEGCLSVPGIYDNVERAEKVRVRALNEKGETFEMDCEGLLAVCIQHEMDHLMGRVFVEYLSSLKQTRIKSKM
KKLAHAM
>Mature_166_residues
ALLNIINYPDKRLHKIAKPVEAVNDRIRRLVKDMAETMYAAPGVGLAATQVDVHERVIVIDVSDDHNELLTFINPEIIWS
SDERKLSEEGCLSVPGIYDNVERAEKVRVRALNEKGETFEMDCEGLLAVCIQHEMDHLMGRVFVEYLSSLKQTRIKSKMK
KLAHAM

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=166, Percent_Identity=28.9156626506024, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789682, Length=163, Percent_Identity=57.6687116564417, Blast_Score=199, Evalue=7e-53,
Organism=Drosophila melanogaster, GI24645728, Length=146, Percent_Identity=32.8767123287671, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19017; Mature: 18886

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLNIINYPDKRLHKIAKPVEAVNDRIRRLVKDMAETMYAAPGVGLAATQVDVHERVIV
CCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHCEEEE
IDVSDDHNELLTFINPEIIWSSDERKLSEEGCLSVPGIYDNVERAEKVRVRALNEKGETF
EEECCCHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
EMDCEGLLAVCIQHEMDHLMGRVFVEYLSSLKQTRIKSKMKKLAHAM
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALLNIINYPDKRLHKIAKPVEAVNDRIRRLVKDMAETMYAAPGVGLAATQVDVHERVIV
CCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHCEEEE
IDVSDDHNELLTFINPEIIWSSDERKLSEEGCLSVPGIYDNVERAEKVRVRALNEKGETF
EEECCCHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
EMDCEGLLAVCIQHEMDHLMGRVFVEYLSSLKQTRIKSKMKKLAHAM
ECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]