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The map label for this gene is nfnB [H]

Identifier: 161621318

GI number: 161621318

Start: 1972681

End: 1973334

Strand: Direct

Name: nfnB [H]

Synonym: Bxe_A2684

Alternate gene names: 161621318

Gene position: 1972681-1973334 (Clockwise)

Preceding gene: 91783122

Following gene: 91783124

Centisome position: 40.29

GC content: 61.31

Gene sequence:

>654_bases
GTGAATATTGCAGGCTATGCGAAGAAGCGGTACTCGACGAAAGCGTTCGATCCCGCGCGCAAGGTGCCCGACGAAACCAT
TGCACAGTTGCGCGAGCTGATTCGTTTCAGCCCGTCGTCGGTGAATTCACAGCCATGGCACTTCGTCGTCGCGTCGAGCG
ACGACGCGAAGGCACGTGTCGCGAAGGCGGCACAGGGTCGCTTCGCTTATAACGAGCCGAAAGTACGCAACGCATCGCAC
GTGTTCGTGCTGTGCGCACGTGTGCTGATCGACGATGTGCATCTTGCGAGTGTGCTGTTGCAGGAAGAGAACGATGGGCG
CTTCGTCAGCGCCGACGCGCGCAACGCGCAGCGGAACTCGCGGCAGGGTTACGTCGACACGCATCGCTACGAGCTAAAGG
ACGCGCAGCACTGGATGGAGAAGCAGGTCTATCTCGCGCTGGGGACGCTGCTGCTCGGCGCAGCAGCGCTCGACGTGGAC
GCCTGCCCGATGGAGGGCTTCGACGCGAAGGTGCTCGACGACGAGCTGGGTTTGCGCGGAAAGGGCTTCACCAGTGTGGC
GCTTGTGGGACTCGGCTATAGCGGAGAGGGCGATTTCAACAAGGCGCTGCCGAAATCCCGTCTGGCGGATGAGGTGGTCT
TTACGGACATTTGA

Upstream 100 bases:

>100_bases
TGCTTGAAGGGTTCTGAGCGAAGCGCTTTACGGGTTGGTCCGGCTGTGCCCCATGCTGCCGGGCCGGCTGACATTTTTAT
CGACGAGGGAGGGCAGCATC

Downstream 100 bases:

>100_bases
GTCCGGTAACGGACCAGCGTGCCGCAGAGTATTCAACGCGCCTGTATATAGCGCACTGTCCGGCTTCATCATGCGGTCGA
CGGGAGATTCACGCTCATGG

Product: dihydropteridine reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 217

Protein sequence:

>217_residues
MNIAGYAKKRYSTKAFDPARKVPDETIAQLRELIRFSPSSVNSQPWHFVVASSDDAKARVAKAAQGRFAYNEPKVRNASH
VFVLCARVLIDDVHLASVLLQEENDGRFVSADARNAQRNSRQGYVDTHRYELKDAQHWMEKQVYLALGTLLLGAAALDVD
ACPMEGFDAKVLDDELGLRGKGFTSVALVGLGYSGEGDFNKALPKSRLADEVVFTDI

Sequences:

>Translated_217_residues
MNIAGYAKKRYSTKAFDPARKVPDETIAQLRELIRFSPSSVNSQPWHFVVASSDDAKARVAKAAQGRFAYNEPKVRNASH
VFVLCARVLIDDVHLASVLLQEENDGRFVSADARNAQRNSRQGYVDTHRYELKDAQHWMEKQVYLALGTLLLGAAALDVD
ACPMEGFDAKVLDDELGLRGKGFTSVALVGLGYSGEGDFNKALPKSRLADEVVFTDI
>Mature_217_residues
MNIAGYAKKRYSTKAFDPARKVPDETIAQLRELIRFSPSSVNSQPWHFVVASSDDAKARVAKAAQGRFAYNEPKVRNASH
VFVLCARVLIDDVHLASVLLQEENDGRFVSADARNAQRNSRQGYVDTHRYELKDAQHWMEKQVYLALGTLLLGAAALDVD
ACPMEGFDAKVLDDELGLRGKGFTSVALVGLGYSGEGDFNKALPKSRLADEVVFTDI

Specific function: Reduction of a variety of nitroaromatic compounds using NADH (and to lesser extent NADPH) as source of reducing equivalents; two electrons are transferred. Capable of reducing nitrofurazone [H]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

Organism=Escherichia coli, GI1786792, Length=217, Percent_Identity=50.2304147465438, Blast_Score=232, Evalue=1e-62,

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.-; 1.5.1.34

Molecular weight: Translated: 23948; Mature: 23948

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIAGYAKKRYSTKAFDPARKVPDETIAQLRELIRFSPSSVNSQPWHFVVASSDDAKARV
CCCCHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHH
AKAAQGRFAYNEPKVRNASHVFVLCARVLIDDVHLASVLLQEENDGRFVSADARNAQRNS
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHCC
RQGYVDTHRYELKDAQHWMEKQVYLALGTLLLGAAALDVDACPMEGFDAKVLDDELGLRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHCCCCC
KGFTSVALVGLGYSGEGDFNKALPKSRLADEVVFTDI
CCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNIAGYAKKRYSTKAFDPARKVPDETIAQLRELIRFSPSSVNSQPWHFVVASSDDAKARV
CCCCHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHH
AKAAQGRFAYNEPKVRNASHVFVLCARVLIDDVHLASVLLQEENDGRFVSADARNAQRNS
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHCC
RQGYVDTHRYELKDAQHWMEKQVYLALGTLLLGAAALDVDACPMEGFDAKVLDDELGLRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEHHHCCCCC
KGFTSVALVGLGYSGEGDFNKALPKSRLADEVVFTDI
CCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2179862; 11677609 [H]