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The map label for this gene is pykA [H]

Identifier: 91790463

GI number: 91790463

Start: 4914754

End: 4916187

Strand: Reverse

Name: pykA [H]

Synonym: Bpro_4634

Alternate gene names: 91790463

Gene position: 4916187-4914754 (Counterclockwise)

Preceding gene: 91790464

Following gene: 91790461

Centisome position: 94.54

GC content: 63.25

Gene sequence:

>1434_bases
ATGCCAAGTCGCGCTACCAAGATCGTTGTCACCCTCGGGCCCGCTTCCAGCGACCCTGTCCTGCTGGAAAAAATGATTCG
CGCAGGTGTCAATGTGGTGCGCCTGAATTTCAGCCATGGCACGGCGCAGGACCATGTCGACCGGGCAAAGCTGGTGCGCG
AAGCCGCCCAGCGCGCCGGCCGTGAAGTGGCCATCATGGCCGACCTGCAGGGCCCGAAAATCCGTGTCGGCAAATTTGCC
GAAGGCAAGGTGCGGCTCGAGGCCGGCGAGAAATTCATCCTCGATGCCTCCCGCACCGAACCCGGCGACCTCAAGGGTGT
GGGTCTGGACTACAAAGAGCTGCCGCGGGATGTGAAGGCGGGCGATGTGCTGCTGCTCAACGACGGCCTGATTGTGCTGA
CGGTGGAGGCGGTGCGCGGCGAGGAAGTGCACACCATCGTCAAGCTCGGCGGCGACCTGTCCAACAACAAGGGCATCAAC
AAGCAGGGCGGCGGCCTGACGGCCCCCGCGCTGACGGCCAAGGACATGGAAGACATCAAGACCGCGATGAGCTTTCGGGC
CGACTATGTGGCCGTGAGCTTTCCAAAAAATGCCACCGACATGGAAATGGCGCGCCAGCTGTGCGATGTGGCGGCCGCGC
CCTACAAGCACAAGCCCGGCCTGATCGCCAAGATCGAGCGGGCCGAAGCCATTCCCAAGCTGGAAGAAATCCTGCGCGTC
AGTGACGCCATCATGGTGGCCCGCGGCGACCTGGCGATTGAAGTGGGCAACGCGGCAGTGCCGGCCCTGCAAAAACGCAT
GATCAAGATGGCCCGGGCGCTCGACAAGGTCGTCATCACTGCCACGCAGATGATGGAAAGCATGATCGTCAATCCGGTAC
CCACGCGCGCCGAGGTCAGCGACGTGGCCAATGCCGTGCTCGACGGCAGCGACGCGGTGATGCTCAGCGCCGAAACCGCA
GCCGGCAAATATCCGCTCGAGACGGTGGAGGAAATGGCCAAGATTTGCGAGGAAGCCGAGAAAGCCGAGTACAAGGAACT
GGAAGCGGACTTCACCGGCAAGACCTTCACCCGTATCGACCAATCGATTGCCATGGGCACCCTGTTCACGGCCCACCACC
TGGGGGCCAAGGCGATCGTGGCGCTGACGGACAGCGGCTCCACGCCTCTCTGGATGAGCCGCCATGACATCCGCGTACCC
ATCTATGCCCTGACCCCGCACCTGGCCACGCAGCGCAGGATGGCGCTTTACCGCAATGTGCGTCCCCTGCTCATGGACAC
CAGCGCCGACCGCGACACCGCTCTGGCACAGGCCGAAGGCCACCTGCTGCGCCGCAATATCGTGCAGAGTGGCGACGTGT
ATGCCATCACCTGCGGTGAGCCGATGGGTGCGCCCGGCGGTACCAACATGCTCAAGATCTGCAGAGTAGCTTAG

Upstream 100 bases:

>100_bases
AGGGGGCTGCGATCCCGCAAGCCCGCGTGCGCGCCGCAGTTGGTACCGGTTTGGTAACTTTTTCGTGTGAAAATCGGGAA
CTAAATTACAGGAGACATTC

Downstream 100 bases:

>100_bases
CCCCCACGCTCCCCCACTTCGTGTGGGTCGCTGCCCCCCGAGGGGGCCGCTGCGCCTGCGGTCTGGCGAAGCCAGTCCCG
CGGCCCCTGCTGGGATCTAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MPSRATKIVVTLGPASSDPVLLEKMIRAGVNVVRLNFSHGTAQDHVDRAKLVREAAQRAGREVAIMADLQGPKIRVGKFA
EGKVRLEAGEKFILDASRTEPGDLKGVGLDYKELPRDVKAGDVLLLNDGLIVLTVEAVRGEEVHTIVKLGGDLSNNKGIN
KQGGGLTAPALTAKDMEDIKTAMSFRADYVAVSFPKNATDMEMARQLCDVAAAPYKHKPGLIAKIERAEAIPKLEEILRV
SDAIMVARGDLAIEVGNAAVPALQKRMIKMARALDKVVITATQMMESMIVNPVPTRAEVSDVANAVLDGSDAVMLSAETA
AGKYPLETVEEMAKICEEAEKAEYKELEADFTGKTFTRIDQSIAMGTLFTAHHLGAKAIVALTDSGSTPLWMSRHDIRVP
IYALTPHLATQRRMALYRNVRPLLMDTSADRDTALAQAEGHLLRRNIVQSGDVYAITCGEPMGAPGGTNMLKICRVA

Sequences:

>Translated_477_residues
MPSRATKIVVTLGPASSDPVLLEKMIRAGVNVVRLNFSHGTAQDHVDRAKLVREAAQRAGREVAIMADLQGPKIRVGKFA
EGKVRLEAGEKFILDASRTEPGDLKGVGLDYKELPRDVKAGDVLLLNDGLIVLTVEAVRGEEVHTIVKLGGDLSNNKGIN
KQGGGLTAPALTAKDMEDIKTAMSFRADYVAVSFPKNATDMEMARQLCDVAAAPYKHKPGLIAKIERAEAIPKLEEILRV
SDAIMVARGDLAIEVGNAAVPALQKRMIKMARALDKVVITATQMMESMIVNPVPTRAEVSDVANAVLDGSDAVMLSAETA
AGKYPLETVEEMAKICEEAEKAEYKELEADFTGKTFTRIDQSIAMGTLFTAHHLGAKAIVALTDSGSTPLWMSRHDIRVP
IYALTPHLATQRRMALYRNVRPLLMDTSADRDTALAQAEGHLLRRNIVQSGDVYAITCGEPMGAPGGTNMLKICRVA
>Mature_476_residues
PSRATKIVVTLGPASSDPVLLEKMIRAGVNVVRLNFSHGTAQDHVDRAKLVREAAQRAGREVAIMADLQGPKIRVGKFAE
GKVRLEAGEKFILDASRTEPGDLKGVGLDYKELPRDVKAGDVLLLNDGLIVLTVEAVRGEEVHTIVKLGGDLSNNKGINK
QGGGLTAPALTAKDMEDIKTAMSFRADYVAVSFPKNATDMEMARQLCDVAAAPYKHKPGLIAKIERAEAIPKLEEILRVS
DAIMVARGDLAIEVGNAAVPALQKRMIKMARALDKVVITATQMMESMIVNPVPTRAEVSDVANAVLDGSDAVMLSAETAA
GKYPLETVEEMAKICEEAEKAEYKELEADFTGKTFTRIDQSIAMGTLFTAHHLGAKAIVALTDSGSTPLWMSRHDIRVPI
YALTPHLATQRRMALYRNVRPLLMDTSADRDTALAQAEGHLLRRNIVQSGDVYAITCGEPMGAPGGTNMLKICRVA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=498, Percent_Identity=37.7510040160643, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI33286420, Length=498, Percent_Identity=37.7510040160643, Blast_Score=274, Evalue=1e-73,
Organism=Homo sapiens, GI10835121, Length=498, Percent_Identity=37.3493975903614, Blast_Score=268, Evalue=7e-72,
Organism=Homo sapiens, GI32967597, Length=497, Percent_Identity=37.0221327967807, Blast_Score=268, Evalue=7e-72,
Organism=Homo sapiens, GI33286418, Length=498, Percent_Identity=37.7510040160643, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI310128732, Length=294, Percent_Identity=37.0748299319728, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI310128730, Length=294, Percent_Identity=37.0748299319728, Blast_Score=161, Evalue=2e-39,
Organism=Homo sapiens, GI310128736, Length=242, Percent_Identity=34.7107438016529, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI310128734, Length=242, Percent_Identity=34.7107438016529, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=34.4036697247706, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=51.0460251046025, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1787965, Length=466, Percent_Identity=38.1974248927039, Blast_Score=289, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17544584, Length=484, Percent_Identity=37.8099173553719, Blast_Score=272, Evalue=3e-73,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=36.8312757201646, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=36.8312757201646, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=36.8312757201646, Blast_Score=242, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=36.8312757201646, Blast_Score=242, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6319279, Length=491, Percent_Identity=34.2158859470468, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324923, Length=435, Percent_Identity=35.1724137931034, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=38.3367139959432, Blast_Score=269, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=38.3367139959432, Blast_Score=268, Evalue=4e-72,
Organism=Drosophila melanogaster, GI24648966, Length=438, Percent_Identity=36.7579908675799, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI24581235, Length=487, Percent_Identity=31.8275154004107, Blast_Score=220, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24646914, Length=275, Percent_Identity=38.1818181818182, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51423; Mature: 51292

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRATKIVVTLGPASSDPVLLEKMIRAGVNVVRLNFSHGTAQDHVDRAKLVREAAQRAG
CCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCC
REVAIMADLQGPKIRVGKFAEGKVRLEAGEKFILDASRTEPGDLKGVGLDYKELPRDVKA
CEEEEEEECCCCEEEECCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCC
GDVLLLNDGLIVLTVEAVRGEEVHTIVKLGGDLSNNKGINKQGGGLTAPALTAKDMEDIK
CCEEEEECCEEEEEEEECCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
TAMSFRADYVAVSFPKNATDMEMARQLCDVAAAPYKHKPGLIAKIERAEAIPKLEEILRV
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHCCHHHHHHHHH
SDAIMVARGDLAIEVGNAAVPALQKRMIKMARALDKVVITATQMMESMIVNPVPTRAEVS
HHHEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
DVANAVLDGSDAVMLSAETAAGKYPLETVEEMAKICEEAEKAEYKELEADFTGKTFTRID
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
QSIAMGTLFTAHHLGAKAIVALTDSGSTPLWMSRHDIRVPIYALTPHLATQRRMALYRNV
HHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHCC
RPLLMDTSADRDTALAQAEGHLLRRNIVQSGDVYAITCGEPMGAPGGTNMLKICRVA
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHEECC
>Mature Secondary Structure 
PSRATKIVVTLGPASSDPVLLEKMIRAGVNVVRLNFSHGTAQDHVDRAKLVREAAQRAG
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCC
REVAIMADLQGPKIRVGKFAEGKVRLEAGEKFILDASRTEPGDLKGVGLDYKELPRDVKA
CEEEEEEECCCCEEEECCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCC
GDVLLLNDGLIVLTVEAVRGEEVHTIVKLGGDLSNNKGINKQGGGLTAPALTAKDMEDIK
CCEEEEECCEEEEEEEECCCCHHEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
TAMSFRADYVAVSFPKNATDMEMARQLCDVAAAPYKHKPGLIAKIERAEAIPKLEEILRV
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEHHHHCCHHHHHHHHH
SDAIMVARGDLAIEVGNAAVPALQKRMIKMARALDKVVITATQMMESMIVNPVPTRAEVS
HHHEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
DVANAVLDGSDAVMLSAETAAGKYPLETVEEMAKICEEAEKAEYKELEADFTGKTFTRID
HHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHH
QSIAMGTLFTAHHLGAKAIVALTDSGSTPLWMSRHDIRVPIYALTPHLATQRRMALYRNV
HHHHHHHHHHHHHCCCEEEEEEECCCCCCEEECCCCCEEEEEEECCCHHHHHHHHHHHCC
RPLLMDTSADRDTALAQAEGHLLRRNIVQSGDVYAITCGEPMGAPGGTNMLKICRVA
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]