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The map label for this gene is gcl [H]

Identifier: 91790391

GI number: 91790391

Start: 4825663

End: 4827459

Strand: Direct

Name: gcl [H]

Synonym: Bpro_4561

Alternate gene names: 91790391

Gene position: 4825663-4827459 (Clockwise)

Preceding gene: 91790389

Following gene: 91790392

Centisome position: 92.8

GC content: 64.72

Gene sequence:

>1797_bases
ATGGCAAAAATGAAGGCTGCAATGGCCGCCGTGCTCGTGATGGAAAAAGAAGGCGTGACCCAGGCCTTTGGCGTGCCGGG
CGCAGCCATCAACCCGCTGTACGCCGCGCTGCGCGAGCGCCAGTCGATTACCCACATCCTGGCGCGTCACGTGGAAGGCG
CCTCCCACATGGCCGAGGGCTACACCCGCGCCAAGGCCGGCAACATTGGCGTGTGCATAGGCACCAGCGGCCCGGCCGGC
ACCGACATGATCACCGGGCTGTACTCGGCCATTGCCGACAGCATTCCGATTTTGTGCATCACCGGCCAGGCGCCGCGCGC
ACGGCTCTACAAGGAAGACTTCCAGGCGGTGGACATCGAATCGATTTCCAAGCCGGTGACCAAGTGGAGCGTGACGGTGC
GCGAGCCGGCCCAGGTGCCGCGCGCCTTCCAGCAGGCCTTTCACCTGATGCGTTCGGGCCGCCCCGGCCCGGTGCTGATT
GACCTGCCGTTCGACGTGCAGATGGCCGAAATCGAATTCGACATCGACACCTACGAACCGCTGCCGGTCTACAAGCCGGC
CGCCAGCCGCAAGCAGATCGAAAAGGCGCTCGACATGCTGGCCGCCGCCTCCAAGCCGCTGATCGTCGCCGGCGGCGGCA
TCATCAATGCCGATGCGACCGACCTGCTGGTCGAGTTCGCCGAGCTGACCGGCGTGCCGGTGATTCCCACGCTGATGGGC
TGGGGCAGCATTCCCGACGACCACCCGCTGATGGCGGGCATGTGCGGCCTGCAGACCAGCCACCGCTACGGCAACGCCAC
CATGCTGGCCAGTGACTTCGTGTTCGGCATCGGCAACCGCTGGGCCAACCGCCACACCGGCTCGACCGAGGTCTACTGCA
AGGGCCGCACCTTTGTCCACGTCGACATCGAACCGACGCAAATCGGCCGCGTGTTCAGCCCCGACTTCGGCATCGTCAGC
GACGCCAAGGCCGCGTTGACGCTGCTGGTCGAGGTGGCCCGCGAGCGCAAGGCGGCCAAGCGGCTGCGGGACTACAGCGA
ATGGGCGGCACGCTGCGCCGAGCGCAAGAAACTGATGCACCGCAAGTCGCACTTCACCGAGACACCGATCAAGCCGATGC
GGGTGTACGAGGAGATGAACAAGGTGTTTCCGCGCGACACGATTTATGTGACGACCATCGGCCTGTCGCAAATTGCCGGC
GCGCAGTTCCTCAACGTCTATGGGCCGCGCCAGTGGATCAACTGCGGCCAGGCCGGCCCGCTGGGCTGGACGATTCCGGC
CGCGCTGGGCGTGGTGGCGGCTGACCCGACGCGCACGGTGGTGGGCCTGTCGGGCGACTACGACTTCCAGTTCCTGATGG
AAGAGCTGGCCGTTGGCGCGCAGTTCAACCTGCCCTATGTGCAGGTGCTGGTGAACAACAGCTACCTGGGCCTGATTCGC
CAGAGCCAGCGCGGCTTCGACATGGACTACTGCGTGCAGCTGGCATTTGAAAACCAGAACGTGCCGGCAGGCGAAGGCGA
TCTGCGCAGCTACGGCGTCGATCACCAGGCAGTCGTGCAGGGCCTGGGCTGCAAATCCCTGCGCGTCACCGATCCCGAGA
AGATTGAATCGGCGATGGTCGAGGCCCAGGCCATGGCGCGCAAATACCGCGTGCCGGTGGTGGTGGAAGTGATCCTGGAA
AAAGTCACCAACATTGCCATGGGCACCGAAATCGACAAGGTCAATGAGTTTGAAGACATTGACTGCCGCCATCCCAAGGG
CCGCGAGGGCCTGGAACTGGCCGGCCTGCTGGAGTAA

Upstream 100 bases:

>100_bases
TCCATTCTGGAATAACCGTAATGCATGCCTTGGGGCTTCCGCGCAAGCGGCAGGCGGGCGACCATCAAGCCAGTTCAAAG
CCACCCCAAGGAGACTCTCA

Downstream 100 bases:

>100_bases
GGCCGCGGCAACGCAGTCATCAACGCCCCCCTACTCACTTATACCCACCCACAGACAAGACATGCCAAGATTTTCCGCCA
ACCTCAGCATGCTCTTCACC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MAKMKAAMAAVLVMEKEGVTQAFGVPGAAINPLYAALRERQSITHILARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAG
TDMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIESISKPVTKWSVTVREPAQVPRAFQQAFHLMRSGRPGPVLI
DLPFDVQMAEIEFDIDTYEPLPVYKPAASRKQIEKALDMLAAASKPLIVAGGGIINADATDLLVEFAELTGVPVIPTLMG
WGSIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSTEVYCKGRTFVHVDIEPTQIGRVFSPDFGIVS
DAKAALTLLVEVARERKAAKRLRDYSEWAARCAERKKLMHRKSHFTETPIKPMRVYEEMNKVFPRDTIYVTTIGLSQIAG
AQFLNVYGPRQWINCGQAGPLGWTIPAALGVVAADPTRTVVGLSGDYDFQFLMEELAVGAQFNLPYVQVLVNNSYLGLIR
QSQRGFDMDYCVQLAFENQNVPAGEGDLRSYGVDHQAVVQGLGCKSLRVTDPEKIESAMVEAQAMARKYRVPVVVEVILE
KVTNIAMGTEIDKVNEFEDIDCRHPKGREGLELAGLLE

Sequences:

>Translated_598_residues
MAKMKAAMAAVLVMEKEGVTQAFGVPGAAINPLYAALRERQSITHILARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAG
TDMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIESISKPVTKWSVTVREPAQVPRAFQQAFHLMRSGRPGPVLI
DLPFDVQMAEIEFDIDTYEPLPVYKPAASRKQIEKALDMLAAASKPLIVAGGGIINADATDLLVEFAELTGVPVIPTLMG
WGSIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSTEVYCKGRTFVHVDIEPTQIGRVFSPDFGIVS
DAKAALTLLVEVARERKAAKRLRDYSEWAARCAERKKLMHRKSHFTETPIKPMRVYEEMNKVFPRDTIYVTTIGLSQIAG
AQFLNVYGPRQWINCGQAGPLGWTIPAALGVVAADPTRTVVGLSGDYDFQFLMEELAVGAQFNLPYVQVLVNNSYLGLIR
QSQRGFDMDYCVQLAFENQNVPAGEGDLRSYGVDHQAVVQGLGCKSLRVTDPEKIESAMVEAQAMARKYRVPVVVEVILE
KVTNIAMGTEIDKVNEFEDIDCRHPKGREGLELAGLLE
>Mature_597_residues
AKMKAAMAAVLVMEKEGVTQAFGVPGAAINPLYAALRERQSITHILARHVEGASHMAEGYTRAKAGNIGVCIGTSGPAGT
DMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIESISKPVTKWSVTVREPAQVPRAFQQAFHLMRSGRPGPVLID
LPFDVQMAEIEFDIDTYEPLPVYKPAASRKQIEKALDMLAAASKPLIVAGGGIINADATDLLVEFAELTGVPVIPTLMGW
GSIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSTEVYCKGRTFVHVDIEPTQIGRVFSPDFGIVSD
AKAALTLLVEVARERKAAKRLRDYSEWAARCAERKKLMHRKSHFTETPIKPMRVYEEMNKVFPRDTIYVTTIGLSQIAGA
QFLNVYGPRQWINCGQAGPLGWTIPAALGVVAADPTRTVVGLSGDYDFQFLMEELAVGAQFNLPYVQVLVNNSYLGLIRQ
SQRGFDMDYCVQLAFENQNVPAGEGDLRSYGVDHQAVVQGLGCKSLRVTDPEKIESAMVEAQAMARKYRVPVVVEVILEK
VTNIAMGTEIDKVNEFEDIDCRHPKGREGLELAGLLE

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=564, Percent_Identity=23.936170212766, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI21361361, Length=612, Percent_Identity=23.5294117647059, Blast_Score=117, Evalue=4e-26,
Organism=Escherichia coli, GI1786717, Length=580, Percent_Identity=74.6551724137931, Blast_Score=902, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=565, Percent_Identity=33.6283185840708, Blast_Score=315, Evalue=7e-87,
Organism=Escherichia coli, GI1790104, Length=476, Percent_Identity=34.2436974789916, Blast_Score=278, Evalue=9e-76,
Organism=Escherichia coli, GI1787096, Length=570, Percent_Identity=24.9122807017544, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788716, Length=423, Percent_Identity=26.7139479905437, Blast_Score=134, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=522, Percent_Identity=25.6704980842912, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=522, Percent_Identity=25.6704980842912, Blast_Score=109, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6323755, Length=598, Percent_Identity=31.2709030100334, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6320816, Length=570, Percent_Identity=24.7368421052632, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323163, Length=478, Percent_Identity=23.4309623430962, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323073, Length=488, Percent_Identity=22.5409836065574, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19922626, Length=584, Percent_Identity=24.8287671232877, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 65374; Mature: 65243

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMKAAMAAVLVMEKEGVTQAFGVPGAAINPLYAALRERQSITHILARHVEGASHMAEG
CCHHHHHHHHHHEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YTRAKAGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCEECHH
SISKPVTKWSVTVREPAQVPRAFQQAFHLMRSGRPGPVLIDLPFDVQMAEIEFDIDTYEP
HHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCC
LPVYKPAASRKQIEKALDMLAAASKPLIVAGGGIINADATDLLVEFAELTGVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHHCCCCCHHHHHC
WGSIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSTEVYCKGRTFVH
CCCCCCCCCHHHHHHCCCCCCCCCCEEEEHHHHHHHCCCHHCCCCCCCCEEEECCCEEEE
VDIEPTQIGRVFSPDFGIVSDAKAALTLLVEVARERKAAKRLRDYSEWAARCAERKKLMH
EECCHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSHFTETPIKPMRVYEEMNKVFPRDTIYVTTIGLSQIAGAQFLNVYGPRQWINCGQAGP
HHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECHHHHCCHHHHHHCCCHHHHCCCCCCC
LGWTIPAALGVVAADPTRTVVGLSGDYDFQFLMEELAVGAQFNLPYVQVLVNNSYLGLIR
CCEEHHHHHHEEECCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEE
QSQRGFDMDYCVQLAFENQNVPAGEGDLRSYGVDHQAVVQGLGCKSLRVTDPEKIESAMV
CCCCCCCHHHEEEEEECCCCCCCCCCCHHHCCCCHHHHHHCCCCCCEECCCHHHHHHHHH
EAQAMARKYRVPVVVEVILEKVTNIAMGTEIDKVNEFEDIDCRHPKGREGLELAGLLE
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKMKAAMAAVLVMEKEGVTQAFGVPGAAINPLYAALRERQSITHILARHVEGASHMAEG
CHHHHHHHHHHEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
YTRAKAGNIGVCIGTSGPAGTDMITGLYSAIADSIPILCITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCEECHH
SISKPVTKWSVTVREPAQVPRAFQQAFHLMRSGRPGPVLIDLPFDVQMAEIEFDIDTYEP
HHCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEEECCCCCC
LPVYKPAASRKQIEKALDMLAAASKPLIVAGGGIINADATDLLVEFAELTGVPVIPTLMG
CCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCEECCCHHHHHHHHHHHCCCCCHHHHHC
WGSIPDDHPLMAGMCGLQTSHRYGNATMLASDFVFGIGNRWANRHTGSTEVYCKGRTFVH
CCCCCCCCCHHHHHHCCCCCCCCCCEEEEHHHHHHHCCCHHCCCCCCCCEEEECCCEEEE
VDIEPTQIGRVFSPDFGIVSDAKAALTLLVEVARERKAAKRLRDYSEWAARCAERKKLMH
EECCHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSHFTETPIKPMRVYEEMNKVFPRDTIYVTTIGLSQIAGAQFLNVYGPRQWINCGQAGP
HHHCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECHHHHCCHHHHHHCCCHHHHCCCCCCC
LGWTIPAALGVVAADPTRTVVGLSGDYDFQFLMEELAVGAQFNLPYVQVLVNNSYLGLIR
CCEEHHHHHHEEECCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEE
QSQRGFDMDYCVQLAFENQNVPAGEGDLRSYGVDHQAVVQGLGCKSLRVTDPEKIESAMV
CCCCCCCHHHEEEEEECCCCCCCCCCCHHHCCCCHHHHHHCCCCCCEECCCHHHHHHHHH
EAQAMARKYRVPVVVEVILEKVTNIAMGTEIDKVNEFEDIDCRHPKGREGLELAGLLE
HHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]