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The map label for this gene is citE [H]

Identifier: 91790386

GI number: 91790386

Start: 4819645

End: 4820469

Strand: Direct

Name: citE [H]

Synonym: Bpro_4556

Alternate gene names: 91790386

Gene position: 4819645-4820469 (Clockwise)

Preceding gene: 91790385

Following gene: 91790387

Centisome position: 92.68

GC content: 63.64

Gene sequence:

>825_bases
ATGCCTGACTGCGAACCCCTGATCTACCTGTTCGTGCCCGGCAACCGCCCCGAGCGCTTCACCAAGGCGCTGGCCTCGGG
GGCTGACCGCATCATCATCGACCTGGAAGACGCTGTAGCGCCCGAATGCAAAGCGGCGGCTCGCACCGCGATTGGGGAAT
GGATGGCTGATAGCGATGCGCCGCGCGAGCGGGTGCTGATACGCATCAATGATGCTGCCACCCCCTGGCATGCCGACGAT
CTAACGCTGGTCAGCACGGTGCGAGCGCTTTGCGTGATGTTGCCGAAATGCGAAACACCCAATCAGGTCGCTGCGGTGCT
GGCCTGTTTACCCAAGGATGCAACCGTGCTGCCGCTGATTGAAACCGCACGCGGCGTGGTGGCCTTGCATGAGATTGCCG
CAGCGCCCGGCGTGACGCGGCTGGCCTTTGGGTCACTGGATTACATGGCGGACCTGGATATTCCCGCAGGCAGCCTGGCA
CTCCAGTTCGCCGCCGCACAAATTGCCATCGCCTCGCGCGCTGCAGGCATTGGATCGCCCGTAGCCGGCGTCACGCCCGA
TCTCGACGCCACGCGCGTGGCCACCGATATGGCGAACGCCCGCACTTTGGGGTTCGGCGCCAAGATGTGCATTCACCCTT
CGCAAGTAGCTGCGGCGCGTACCGCCCTGGCGCCCAGCGCCCAGGAGCTGGCTTGGGCACAGCGTGTACTGCAAGCCTGG
AATACGTCTGCCGGCGACGCAATACAGGTGGACGGCAAAATGGTGGACCGCCCAATCGTCCTGAAAGCTGAGCGCATCGT
TGCACAAGCAGCCCGAACGAATTAA

Upstream 100 bases:

>100_bases
CCGTGCCCGCACTGGGCCAGCACACCGATGCGATCCTGGGCCACTATGGTTACAGCGCAGAGCGCATTGCGGATCTGCGC
GCTGAAGGTGTCGTGTGACC

Downstream 100 bases:

>100_bases
GTCCAGGCCCCAATAAAAACATCAATCCAAACGGAGAACCCCATGCCCGCAAGCATCATTGATTCCAGAATTTTCGGCGA
CATGTTCAGCGACGCCAAAA

Product: HpcH/HpaI aldolase

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MPDCEPLIYLFVPGNRPERFTKALASGADRIIIDLEDAVAPECKAAARTAIGEWMADSDAPRERVLIRINDAATPWHADD
LTLVSTVRALCVMLPKCETPNQVAAVLACLPKDATVLPLIETARGVVALHEIAAAPGVTRLAFGSLDYMADLDIPAGSLA
LQFAAAQIAIASRAAGIGSPVAGVTPDLDATRVATDMANARTLGFGAKMCIHPSQVAAARTALAPSAQELAWAQRVLQAW
NTSAGDAIQVDGKMVDRPIVLKAERIVAQAARTN

Sequences:

>Translated_274_residues
MPDCEPLIYLFVPGNRPERFTKALASGADRIIIDLEDAVAPECKAAARTAIGEWMADSDAPRERVLIRINDAATPWHADD
LTLVSTVRALCVMLPKCETPNQVAAVLACLPKDATVLPLIETARGVVALHEIAAAPGVTRLAFGSLDYMADLDIPAGSLA
LQFAAAQIAIASRAAGIGSPVAGVTPDLDATRVATDMANARTLGFGAKMCIHPSQVAAARTALAPSAQELAWAQRVLQAW
NTSAGDAIQVDGKMVDRPIVLKAERIVAQAARTN
>Mature_273_residues
PDCEPLIYLFVPGNRPERFTKALASGADRIIIDLEDAVAPECKAAARTAIGEWMADSDAPRERVLIRINDAATPWHADDL
TLVSTVRALCVMLPKCETPNQVAAVLACLPKDATVLPLIETARGVVALHEIAAAPGVTRLAFGSLDYMADLDIPAGSLAL
QFAAAQIAIASRAAGIGSPVAGVTPDLDATRVATDMANARTLGFGAKMCIHPSQVAAARTALAPSAQELAWAQRVLQAWN
TSAGDAIQVDGKMVDRPIVLKAERIVAQAARTN

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=288, Percent_Identity=25.6944444444444, Blast_Score=92, Evalue=7e-19,
Organism=Escherichia coli, GI87081764, Length=278, Percent_Identity=29.136690647482, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17557458, Length=298, Percent_Identity=24.8322147651007, Blast_Score=77, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 28777; Mature: 28646

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDCEPLIYLFVPGNRPERFTKALASGADRIIIDLEDAVAPECKAAARTAIGEWMADSDA
CCCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCC
PRERVLIRINDAATPWHADDLTLVSTVRALCVMLPKCETPNQVAAVLACLPKDATVLPLI
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEHHH
ETARGVVALHEIAAAPGVTRLAFGSLDYMADLDIPAGSLALQFAAAQIAIASRAAGIGSP
HHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VAGVTPDLDATRVATDMANARTLGFGAKMCIHPSQVAAARTALAPSAQELAWAQRVLQAW
CCCCCCCCCHHHHHHHHHCCEEECCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NTSAGDAIQVDGKMVDRPIVLKAERIVAQAARTN
CCCCCCEEEECCEECCCCEEEHHHHHHHHHHCCC
>Mature Secondary Structure 
PDCEPLIYLFVPGNRPERFTKALASGADRIIIDLEDAVAPECKAAARTAIGEWMADSDA
CCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHCCCCC
PRERVLIRINDAATPWHADDLTLVSTVRALCVMLPKCETPNQVAAVLACLPKDATVLPLI
CCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEHHH
ETARGVVALHEIAAAPGVTRLAFGSLDYMADLDIPAGSLALQFAAAQIAIASRAAGIGSP
HHHHHHHHHHHHHHCCCCHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VAGVTPDLDATRVATDMANARTLGFGAKMCIHPSQVAAARTALAPSAQELAWAQRVLQAW
CCCCCCCCCHHHHHHHHHCCEEECCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHH
NTSAGDAIQVDGKMVDRPIVLKAERIVAQAARTN
CCCCCCEEEECCEECCCCEEEHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]