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The map label for this gene is pcaB [H]

Identifier: 91790387

GI number: 91790387

Start: 4820512

End: 4821861

Strand: Direct

Name: pcaB [H]

Synonym: Bpro_4557

Alternate gene names: 91790387

Gene position: 4820512-4821861 (Clockwise)

Preceding gene: 91790386

Following gene: 91790388

Centisome position: 92.7

GC content: 62.37

Gene sequence:

>1350_bases
ATGCCCGCAAGCATCATTGATTCCAGAATTTTCGGCGACATGTTCAGCGACGCCAAAATGCGCCAGGTCTGGTCCGACGA
AAACCGTACCGCCAAGTACCTCGACATCGAACGCGCGCTCGCCAAGGTGCAGGGCGAACTCGGCCTCATCCCCAGGGAAG
CGGCCGACGAGATCGTCAAGAACTGCAATATCGACCAGATTGACTGGGCCCAGCTGAAGACCAAGACCGAACAGATCGGC
TATCCCATCATCGCGGTAGTCAACCAGATCAATGCCAACTGCAGGGACAAGCTGGGCGAATACTGCCACTGGGGCGCCAC
CACACAAGACATCACCGACACGGCCGCCGTGCTGCAAATCCGCGAGGGCCTGGCGCTGGTCGAAGAAGACCTGAAAGACA
TCTCGGCGGCCCTCGCCAACCTGGCCCGCACGCACCGCAACACACCCGTCATTGGCCGCAGCAACCTGCAGCAGGCCACG
CCCATCACCTTCGGCTACAAGATGGCCAGCATCCTGGCCGGCATTGAACGCCATCGCGAGCGCCTGCAGCAACTCAAACC
CCGCGTGCTGGTGGGTGAGTTTGGCGGTGCGTCGGGAACCCTGTCCTCGCTGGAAAAAGGCGCCATGGAAACCCAGGCCG
GGCTGATGAGAGAGCTGGGCCTGGGCCAGCCGCTGATTTCATGGCACACGGTGCGTGACAACTTCGCCGAAGTCGGCGCC
TTCCTCGGGCTGGTCGGCGGCTCGCTCGGCAAGATAGCCATGGACGTCAAGCTGATGATGCAGACGGAAGTGGCCGAGGT
CTATGAGCCCTTCGCGCCGGGGCGCGGCTCGTCGTCCACGATGCCGCAAAAGCGCAACCCGATCTCGTGCCTCTACATCC
ACGCCAACATCTCGGTCGTGCGCCAGCTGGCCGCCTCCCTGATGGATGCCATGGTGGCCGACCATGAGCGCTCGACCGGG
CCGTGGGAAATCGAATGGGTCGCGATGCCCGAAATCTTCTGCCTCATGTCGGGCGCGCTCAAGCAGACGAAATTCATCCT
GAGCGGCCTGGAAGTCGATGCGGTGCAGATGCGCCGCAACATCGACATGACGCACGGGCTGGTGATGTCCGAGGCCGTGA
TGATGGGCCTGGGGCCCTTCATTGGCCGCGAATACGCCCACGACCTGGTGTATGACATCTGCCGCGAGGCGCTCAAGCTC
AAACGCCCACTGATCGAACTGCTGGCCGAGCACCCTGAAATCAATAAACATGTCACGCGCGAACAGCTGGCCTCCTTCTG
CGACCCGATGAACAACCTGGGCCAGGCCGGCGTCATGGTGGACCGGGTATTGGCGGCGCGCAAAGCCTGA

Upstream 100 bases:

>100_bases
CCGCCCAATCGTCCTGAAAGCTGAGCGCATCGTTGCACAAGCAGCCCGAACGAATTAAGTCCAGGCCCCAATAAAAACAT
CAATCCAAACGGAGAACCCC

Downstream 100 bases:

>100_bases
TCTCAAGAATTTTTAACCCTCAGAAAAAAGAAAGAGACAAACGCATGAAAACTCACTCCACCGGCTGCCGCATCCCGCTG
CTTGGTCTGTCATTGCTGGC

Product: fumarate lyase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MPASIIDSRIFGDMFSDAKMRQVWSDENRTAKYLDIERALAKVQGELGLIPREAADEIVKNCNIDQIDWAQLKTKTEQIG
YPIIAVVNQINANCRDKLGEYCHWGATTQDITDTAAVLQIREGLALVEEDLKDISAALANLARTHRNTPVIGRSNLQQAT
PITFGYKMASILAGIERHRERLQQLKPRVLVGEFGGASGTLSSLEKGAMETQAGLMRELGLGQPLISWHTVRDNFAEVGA
FLGLVGGSLGKIAMDVKLMMQTEVAEVYEPFAPGRGSSSTMPQKRNPISCLYIHANISVVRQLAASLMDAMVADHERSTG
PWEIEWVAMPEIFCLMSGALKQTKFILSGLEVDAVQMRRNIDMTHGLVMSEAVMMGLGPFIGREYAHDLVYDICREALKL
KRPLIELLAEHPEINKHVTREQLASFCDPMNNLGQAGVMVDRVLAARKA

Sequences:

>Translated_449_residues
MPASIIDSRIFGDMFSDAKMRQVWSDENRTAKYLDIERALAKVQGELGLIPREAADEIVKNCNIDQIDWAQLKTKTEQIG
YPIIAVVNQINANCRDKLGEYCHWGATTQDITDTAAVLQIREGLALVEEDLKDISAALANLARTHRNTPVIGRSNLQQAT
PITFGYKMASILAGIERHRERLQQLKPRVLVGEFGGASGTLSSLEKGAMETQAGLMRELGLGQPLISWHTVRDNFAEVGA
FLGLVGGSLGKIAMDVKLMMQTEVAEVYEPFAPGRGSSSTMPQKRNPISCLYIHANISVVRQLAASLMDAMVADHERSTG
PWEIEWVAMPEIFCLMSGALKQTKFILSGLEVDAVQMRRNIDMTHGLVMSEAVMMGLGPFIGREYAHDLVYDICREALKL
KRPLIELLAEHPEINKHVTREQLASFCDPMNNLGQAGVMVDRVLAARKA
>Mature_448_residues
PASIIDSRIFGDMFSDAKMRQVWSDENRTAKYLDIERALAKVQGELGLIPREAADEIVKNCNIDQIDWAQLKTKTEQIGY
PIIAVVNQINANCRDKLGEYCHWGATTQDITDTAAVLQIREGLALVEEDLKDISAALANLARTHRNTPVIGRSNLQQATP
ITFGYKMASILAGIERHRERLQQLKPRVLVGEFGGASGTLSSLEKGAMETQAGLMRELGLGQPLISWHTVRDNFAEVGAF
LGLVGGSLGKIAMDVKLMMQTEVAEVYEPFAPGRGSSSTMPQKRNPISCLYIHANISVVRQLAASLMDAMVADHERSTGP
WEIEWVAMPEIFCLMSGALKQTKFILSGLEVDAVQMRRNIDMTHGLVMSEAVMMGLGPFIGREYAHDLVYDICREALKLK
RPLIELLAEHPEINKHVTREQLASFCDPMNNLGQAGVMVDRVLAARKA

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17508577, Length=395, Percent_Identity=21.7721518987342, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32564234, Length=277, Percent_Identity=22.3826714801444, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323391, Length=456, Percent_Identity=24.1228070175439, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24647570, Length=290, Percent_Identity=23.7931034482759, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 49614; Mature: 49483

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPASIIDSRIFGDMFSDAKMRQVWSDENRTAKYLDIERALAKVQGELGLIPREAADEIVK
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
NCNIDQIDWAQLKTKTEQIGYPIIAVVNQINANCRDKLGEYCHWGATTQDITDTAAVLQI
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
REGLALVEEDLKDISAALANLARTHRNTPVIGRSNLQQATPITFGYKMASILAGIERHRE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLQQLKPRVLVGEFGGASGTLSSLEKGAMETQAGLMRELGLGQPLISWHTVRDNFAEVGA
HHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FLGLVGGSLGKIAMDVKLMMQTEVAEVYEPFAPGRGSSSTMPQKRNPISCLYIHANISVV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
RQLAASLMDAMVADHERSTGPWEIEWVAMPEIFCLMSGALKQTKFILSGLEVDAVQMRRN
HHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
IDMTHGLVMSEAVMMGLGPFIGREYAHDLVYDICREALKLKRPLIELLAEHPEINKHVTR
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EQLASFCDPMNNLGQAGVMVDRVLAARKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PASIIDSRIFGDMFSDAKMRQVWSDENRTAKYLDIERALAKVQGELGLIPREAADEIVK
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
NCNIDQIDWAQLKTKTEQIGYPIIAVVNQINANCRDKLGEYCHWGATTQDITDTAAVLQI
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
REGLALVEEDLKDISAALANLARTHRNTPVIGRSNLQQATPITFGYKMASILAGIERHRE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RLQQLKPRVLVGEFGGASGTLSSLEKGAMETQAGLMRELGLGQPLISWHTVRDNFAEVGA
HHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
FLGLVGGSLGKIAMDVKLMMQTEVAEVYEPFAPGRGSSSTMPQKRNPISCLYIHANISVV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHH
RQLAASLMDAMVADHERSTGPWEIEWVAMPEIFCLMSGALKQTKFILSGLEVDAVQMRRN
HHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
IDMTHGLVMSEAVMMGLGPFIGREYAHDLVYDICREALKLKRPLIELLAEHPEINKHVTR
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
EQLASFCDPMNNLGQAGVMVDRVLAARKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]