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The map label for this gene is tuf1 [H]

Identifier: 91790282

GI number: 91790282

Start: 4702549

End: 4703739

Strand: Reverse

Name: tuf1 [H]

Synonym: Bpro_4449

Alternate gene names: 91790282

Gene position: 4703739-4702549 (Counterclockwise)

Preceding gene: 91790283

Following gene: 91790281

Centisome position: 90.45

GC content: 62.89

Gene sequence:

>1191_bases
ATGGCAAAAGGCAAATTTGAGCGGACCAAGCCGCACGTCAACGTCGGCACGATTGGCCACGTGGACCATGGCAAGACGAC
CCTGACGGCGGCGATCACGACCGTGCTGGCATCCAAGTTCGGCGGCGAAGCCAAAGGTTACGACCAGATTGACGCGGCCC
CCGAAGAAAAGGCCCGCGGCATTACGATTAACACCGCCCACGTCGAGTACGAGACGGCCAATCGCCACTACGCCCACGTT
GACTGCCCCGGCCACGCCGACTACGTCAAGAACATGATCACCGGCGCGGCCCAGATGGACGGCGCCATTCTCGTTTGTTC
GGCTGCTGACGGCCCCATGCCCCAGACCCGCGAGCACATCCTGCTGGCCCGCCAGGTCGGCGTGCCCTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTCGACGACGCCGAACTGCTCGAACTCGTCGAGATGGAAGTGCGCGAGCTGCTCGACAAG
TACGATTTCCCTGGCGACAAGACCCCCATCATCCACGGCTCCGCCAAGCTCGCCATGGAAGGCGACAAGGGTCCCCTGGG
CGAGCAGGCCATCATGAAGCTGGCCGACGCCCTGGACACCTACATTCCCCTGCCCGAGCGCGCAGTCGACGGCGCCTTCC
TGATGCCCGTCGAAGACGTGTTCTCCATCTCCGGCCGCGGCACCGTCGTGACCGGCCGTGTCGAGCGCGGCGTGATCAAG
GTCGGCGAAGAGATCGAGATCGTCGGTATTGCCGACACGCAAAAGACCACCTGCACCGGCGTCGAGATGTTCAGGAAGCT
GCTCGATCAGGGCCAGGCCGGCGACAACGTCGGTATTTTGCTGCGCGGCACCAAGCGCGAAGACGTGCAGCGCGGCCAGG
TGCTGTGCAAGCCCGGCTCGATCAAGCCGCATACCCACTTCACCGGCGAGATCTACGTGCTGAGCAAGGACGAAGGCGGC
CGCCACACGCCCTTCTTCAACAACTACCGCCCCCAGTTTTACTTCCGCACGACGGACGTGACCGGCGCGATCGAGTTGCC
AGAAGGCAAGGAAATGGTCATGCCAGGCGACAACGTGTCGATCACCGTCAAGCTGATCAACCCGATCGCCATGGAAGAGG
GCCTGCGCTTTGCCATCCGCGAAGGCGGCAAGACCGTTGGTGCTGGCGTTGTGGCCAAGATCATTGCCTGA

Upstream 100 bases:

>100_bases
GATTCCCGCAAAGGGCACCACATTTTGAGTCGGCGCGGCGCGCTGTGCGCGGTTGCCGGCTGTAGGCGTTAACTGGTTTT
GTAACTTTTCGGAGTTGAAA

Downstream 100 bases:

>100_bases
GTTTTGAAGGTTTGATGGAGGGGTATAGCTCAATTGGCAGAGCGTCGGTCTCCAAAACCGAAGGTTGTAGGTTCGATTCC
TACTGCCCCTGCCACCCGGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLASKFGGEAKGYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLDK
YDFPGDKTPIIHGSAKLAMEGDKGPLGEQAIMKLADALDTYIPLPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQVLCKPGSIKPHTHFTGEIYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGAIELPEGKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGKTVGAGVVAKIIA

Sequences:

>Translated_396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLASKFGGEAKGYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLDK
YDFPGDKTPIIHGSAKLAMEGDKGPLGEQAIMKLADALDTYIPLPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQVLCKPGSIKPHTHFTGEIYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGAIELPEGKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGKTVGAGVVAKIIA
>Mature_395_residues
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLASKFGGEAKGYDQIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLDKY
DFPGDKTPIIHGSAKLAMEGDKGPLGEQAIMKLADALDTYIPLPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIKV
GEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQVLCKPGSIKPHTHFTGEIYVLSKDEGGR
HTPFFNNYRPQFYFRTTDVTGAIELPEGKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGKTVGAGVVAKIIA

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=395, Percent_Identity=56.7088607594937, Blast_Score=480, Evalue=1e-135,
Organism=Homo sapiens, GI4503475, Length=442, Percent_Identity=29.6380090497738, Blast_Score=155, Evalue=5e-38,
Organism=Homo sapiens, GI223555963, Length=433, Percent_Identity=28.4064665127021, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI5729864, Length=433, Percent_Identity=28.4064665127021, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.6380090497738, Blast_Score=148, Evalue=9e-36,
Organism=Homo sapiens, GI194018522, Length=438, Percent_Identity=28.7671232876712, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI46094014, Length=438, Percent_Identity=27.8538812785388, Blast_Score=125, Evalue=5e-29,
Organism=Homo sapiens, GI194097354, Length=438, Percent_Identity=28.7671232876712, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI194018520, Length=438, Percent_Identity=28.7671232876712, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI54607086, Length=320, Percent_Identity=28.75, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=28.7313432835821, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=31.8518518518519, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=396, Percent_Identity=79.5454545454545, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=79.746835443038, Blast_Score=629, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=372, Percent_Identity=29.3010752688172, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI48994988, Length=279, Percent_Identity=29.0322580645161, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1789108, Length=146, Percent_Identity=32.8767123287671, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=52.3573200992556, Blast_Score=414, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.4444444444444, Blast_Score=329, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=29.2792792792793, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=29.2792792792793, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=431, Percent_Identity=28.0742459396752, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=299, Percent_Identity=29.4314381270903, Blast_Score=128, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=25.7990867579909, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=25.7990867579909, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32566629, Length=349, Percent_Identity=28.080229226361, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=212, Percent_Identity=30.6603773584906, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=268, Percent_Identity=26.4925373134328, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=26.5822784810127, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=395, Percent_Identity=65.5696202531646, Blast_Score=531, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=29.2792792792793, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=29.2792792792793, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=340, Percent_Identity=28.8235294117647, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=268, Percent_Identity=27.6119402985075, Blast_Score=83, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6322937, Length=355, Percent_Identity=25.0704225352113, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323098, Length=135, Percent_Identity=32.5925925925926, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281363316, Length=394, Percent_Identity=58.3756345177665, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI17864358, Length=394, Percent_Identity=58.3756345177665, Blast_Score=484, Evalue=1e-137,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.2953367875648, Blast_Score=407, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=29.6213808463252, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=29.6213808463252, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=2e-37,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=30.791788856305, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=28.5245901639344, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI45550900, Length=435, Percent_Identity=28.0459770114943, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.2527881040892, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.2527881040892, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28572034, Length=125, Percent_Identity=40.8, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42941; Mature: 42810

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLASKFGGEAKGYDQIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLDKYDFPGDKTPIIHGSAKLAME
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEE
GDKGPLGEQAIMKLADALDTYIPLPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQVLCKPGS
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCEEECCCC
IKPHTHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPEGKEMVMPGDNVS
CCCCCEECEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCE
ITVKLINPIAMEEGLRFAIREGGKTVGAGVVAKIIA
EEEEEECCHHHHHCCEEEECCCCCEECHHHHHHHCC
>Mature Secondary Structure 
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLASKFGGEAKGYDQIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLDKYDFPGDKTPIIHGSAKLAME
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEE
GDKGPLGEQAIMKLADALDTYIPLPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVIK
CCCCCCCHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCCCCCEEE
VGEEIEIVGIADTQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVQRGQVLCKPGS
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHCCCCEEECCCC
IKPHTHFTGEIYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGAIELPEGKEMVMPGDNVS
CCCCCEECEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCE
ITVKLINPIAMEEGLRFAIREGGKTVGAGVVAKIIA
EEEEEECCHHHHHCCEEEECCCCCEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA