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The map label for this gene is potB [H]

Identifier: 91790251

GI number: 91790251

Start: 4663799

End: 4664671

Strand: Reverse

Name: potB [H]

Synonym: Bpro_4418

Alternate gene names: 91790251

Gene position: 4664671-4663799 (Counterclockwise)

Preceding gene: 91790252

Following gene: 91790250

Centisome position: 89.7

GC content: 56.93

Gene sequence:

>873_bases
ATGGCTGATGCCCTGTCACCCCGTCCCAGTGCACCCTGGGTACTGTCCTCGCCTGCGCTCCTGCTGTTTGTAGGCATCTT
GTTGGTGCCCCTGGCGCTGACCGCAGTGCTGTCGTTCAATGCTTTCGACGGCATGCGCGGCATTCAGCCCAATTACACCC
TGGCCAACTATGCGGAAATACTCACAGACAGCTATTACCACCAGATATTCGTCCGAACCGGCCTCATGGCCTTGGGTGTC
ACGGCGCTGTGTGTGCTGCTTGGCGTGCCGGAAACGCTCATCCTGGCTCGCATGCAGGCGCCCTGGCGTGGTCTATTCCT
TGTCGTCATCCTCGGGCCGCTTCTGATATCGGTGGTCGTACGCACACTTGGCTGGTCGATTCTGCTGGGCAGCCAAGGCA
TGATCAATAATGCCCTGCAAAGCCTTGGCGTCATCGACGGACCCATACGTTTTCTCTTTTCGATGTTGGGTGTCGTGATT
GCTTTGACGCACGTGTTTGTGCCCTTCATGGTCATTGCAGTATGGAGTGCGCTGCAAAAGCTGGATCCGCAGGTAGAACA
CGCCGGTCGATCCTTAGGGGCAACGCCGTCGACTTCGTTTTTGCGTATCGTGTTTCCGCAATTGCTGCCTGGCGTGCTTT
CGGGAAGCATCATTGTTTTTGCACTCGCCGCCTCGGCCTTTGCCACGCCATCGATTATTGGCGGCCGTCGTGTCAAGGTG
GTCGCCACGGCCACCTACGACGAGTTCCTCAACTCGATGAACTGGCCGCTGGGGGCGGCTATCGCAGTGCTACTTCTTTT
GGCCAATGTCGTCATTATTGTGGGTTGCAACCGCATGCTTGAGCGCCGCTTTAAACAGGTATTCGCATCATGA

Upstream 100 bases:

>100_bases
ACATCGACAAATCTGGGCGACCACCTTCCCGGAGAAGGCGAGAACATCGGCCTGGTCTGGCGCGATGACGACCTGCGTGC
CATCCCCAGCGAGCGTGCCC

Downstream 100 bases:

>100_bases
AAGGCGAGATCGGAAGAAACGGCTGGCTGGCCTTGCTGTATCACGCGTGCTTTGTGATTTTTATCGTCGCGCCGCTCATC
GTGGTGGCGTTGGTCTCGTT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MADALSPRPSAPWVLSSPALLLFVGILLVPLALTAVLSFNAFDGMRGIQPNYTLANYAEILTDSYYHQIFVRTGLMALGV
TALCVLLGVPETLILARMQAPWRGLFLVVILGPLLISVVVRTLGWSILLGSQGMINNALQSLGVIDGPIRFLFSMLGVVI
ALTHVFVPFMVIAVWSALQKLDPQVEHAGRSLGATPSTSFLRIVFPQLLPGVLSGSIIVFALAASAFATPSIIGGRRVKV
VATATYDEFLNSMNWPLGAAIAVLLLLANVVIIVGCNRMLERRFKQVFAS

Sequences:

>Translated_290_residues
MADALSPRPSAPWVLSSPALLLFVGILLVPLALTAVLSFNAFDGMRGIQPNYTLANYAEILTDSYYHQIFVRTGLMALGV
TALCVLLGVPETLILARMQAPWRGLFLVVILGPLLISVVVRTLGWSILLGSQGMINNALQSLGVIDGPIRFLFSMLGVVI
ALTHVFVPFMVIAVWSALQKLDPQVEHAGRSLGATPSTSFLRIVFPQLLPGVLSGSIIVFALAASAFATPSIIGGRRVKV
VATATYDEFLNSMNWPLGAAIAVLLLLANVVIIVGCNRMLERRFKQVFAS
>Mature_289_residues
ADALSPRPSAPWVLSSPALLLFVGILLVPLALTAVLSFNAFDGMRGIQPNYTLANYAEILTDSYYHQIFVRTGLMALGVT
ALCVLLGVPETLILARMQAPWRGLFLVVILGPLLISVVVRTLGWSILLGSQGMINNALQSLGVIDGPIRFLFSMLGVVIA
LTHVFVPFMVIAVWSALQKLDPQVEHAGRSLGATPSTSFLRIVFPQLLPGVLSGSIIVFALAASAFATPSIIGGRRVKVV
ATATYDEFLNSMNWPLGAAIAVLLLLANVVIIVGCNRMLERRFKQVFAS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=228, Percent_Identity=33.7719298245614, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1787080, Length=240, Percent_Identity=30, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1787713, Length=209, Percent_Identity=30.1435406698565, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788764, Length=236, Percent_Identity=25, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31126; Mature: 30995

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADALSPRPSAPWVLSSPALLLFVGILLVPLALTAVLSFNAFDGMRGIQPNYTLANYAEI
CCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LTDSYYHQIFVRTGLMALGVTALCVLLGVPETLILARMQAPWRGLFLVVILGPLLISVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RTLGWSILLGSQGMINNALQSLGVIDGPIRFLFSMLGVVIALTHVFVPFMVIAVWSALQK
HHHCHHHEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDPQVEHAGRSLGATPSTSFLRIVFPQLLPGVLSGSIIVFALAASAFATPSIIGGRRVKV
HCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEE
VATATYDEFLNSMNWPLGAAIAVLLLLANVVIIVGCNRMLERRFKQVFAS
EEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ADALSPRPSAPWVLSSPALLLFVGILLVPLALTAVLSFNAFDGMRGIQPNYTLANYAEI
CCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
LTDSYYHQIFVRTGLMALGVTALCVLLGVPETLILARMQAPWRGLFLVVILGPLLISVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RTLGWSILLGSQGMINNALQSLGVIDGPIRFLFSMLGVVIALTHVFVPFMVIAVWSALQK
HHHCHHHEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDPQVEHAGRSLGATPSTSFLRIVFPQLLPGVLSGSIIVFALAASAFATPSIIGGRRVKV
HCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCEEEE
VATATYDEFLNSMNWPLGAAIAVLLLLANVVIIVGCNRMLERRFKQVFAS
EEECHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]