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The map label for this gene is fixI [H]

Identifier: 91790145

GI number: 91790145

Start: 4538890

End: 4541172

Strand: Reverse

Name: fixI [H]

Synonym: Bpro_4307

Alternate gene names: 91790145

Gene position: 4541172-4538890 (Counterclockwise)

Preceding gene: 91790149

Following gene: 91790144

Centisome position: 87.33

GC content: 71.05

Gene sequence:

>2283_bases
ATGCAAACTGCCACCCTTGCCCTGGGCACAGAGCCTGTTGCCCCGATCAATGCCGCCTGGGCTGCGCTGGACGAGCGCGC
CGAATGGGAGGGCTTCAGCCGGCCCGTGGCCGGGCGCGATGGCTGGTGGGAGACGTACCTCGCGATTGACGGCATGCACT
GCGCTGCCTGCTCGCTCACGGTGGAGGAAGCACTCGGCCAGTTGCCGGACGTCGACGGCGTACAGGTCAATGGGGCGACC
GCGATGGCGCGCCTCGTGTGGCGGCCCGGGCGCACCCGCCCCTCGGCCTGGATGGCCGCGCTGCAGCGGGCCGGCTACAC
GGCGCTGCCGGCGGGCGACATGCTGCTGGCCGCGCCGCGGCTGCAGGCCCAGCGCCTGATGCTCTGGCGCTGGCTGGTCG
CGGGCTTTTGCATGATGCAGGTGATGATGTATGCCGTGCCCGCCTACGTGGCCGGCCCCGGCGAGATCACGCCGGATGTG
CAGGCGCTGCTGCGCTGGGCCTCCTGGGTGCTGACGTTGCCGGTCGTGCTGTTTTCCTGCTGGCCGTTCTTTGCCACGGC
GCTGCGTGACCTGCGCGGCCGGCGCATCGGCATGGATGTGCCGGTGGCGCTGGGCATCCTCATCACCTTTGGTGCCAGCA
CGGCCGCCACCTTCGATGCTTCCGGGCCGTGGGGTGCCGAGGTCTGGTACGACTCGTTGACCATGTTCGTGTTCTTCCTG
TTGTCCGGCCGCCTGCTGGAGCAGCGCCTGCGTGACCGCACGGCCGGCTCGCTGGAGGCGCTGATGCGCAGGCTGCCCGA
CAGTGTGGAGCGGCAAGGGGCCGACGGCCAGTATGAGCGCGTGGCCCTGCGGCGCCTCGTCGTGGGCGATCTGATCCGTG
TGCTGCCGGGCGAAGTCATTCCCGCCGACGGCACGGTGACTGCGGGCGACAGCCGGGTGGACGAAGCCCTGCTGACGGGT
GAATCCACGCCGCTGCAGCGGTGCGCCGGTGGCGCGGTGATCGCCGGCAGCCACAACCTCTCGGGCCTGCTGCTGGTGCG
GGTGGAGCGCGTGGGTGCTGAGACGCGGTATGCCAGCATCGTGGCGCTGATGGAGCAGGCCTCGGTGCAAAAGCCGCGGC
TGGCCCAGGTTGCCGATCGCATTGCCAACCCCTTCCTGCTGGGGGTGCTGCTGGCCAGCGCCGCTGCGGCGTTGTGGTGG
TGGCCGGTGGATCCGGCGCATGCCATCAGCGTGGCGGTTGCCGTCCTCATCGTCACCTGCCCCTGCGCGCTGTCGCTGGC
CACGCCGGCCGCCACGCTGGCCGCGGCAGGCGCGCTGGCGCGCCGCGGCGTGCTGGTGCGCCGCCTGCAGGCGCTGGAGT
CCGGCGCATCGGTGGACACGGTGGTGTTCGACAAGACCGGCACGCTGACCGAGGACCGTATGGCGGTGCTCATGCGCCGG
GTCCGCCCGGGCACAGACCCGACACAGGCCCTGCATCTTGCGGCCGCGCTGGCGCAGCATTCGCACCACCCGGTGTCTCG
CGCGATTGTGCAGGCCGTTGCTGGCGCAGTACCAGGCGCGGAGAACATCACGGAGATAGCCGGCCATGGACTGCAGGGCC
AGATCGCCGGGGGTGCGGCAGGGCGCGTGTTGCGGCTGGGTTCGGCCGCATTTTGCGGCGCGCCGCTGCAGGATACGCCG
GCCCATGGCCCGCAGGTGCACCTGGCCGACGAGCGGGGCTGGCTGGCCAGCTTCGAATTTGAGGAGGCGTTGCGGCCCGA
TGCCAGGCCTGCGGTCGGCAGGCTGCAGGCGATGGGCCTGCGGGTGCTGCTTCTCTCGGGCGACCAGGGTGGTGCCGTGT
TGCGGCTGGCCGCCCGCGCTGGTATCGATGAGGCCTACGGTCAGCGCACACCGGAGGACAAGCTGGCGCATGTGCGCTCC
CTGCAACTGCGCGGCCGGCGCGTTGCGATGGTGGGCGACGGCATGAACGACGGGCCGGTGCTGGCCTGCGCAGACCTGTC
GGTGGCCATGGGGCACGCCGTGCCGCTGGCACAGGCGAAGTCCGACTTCGTGGTGCTGGGCAGCCGGCTGTCCGATGTGC
CTGCCTTGCTGCTGCAGGCGCGCCGCACGCGGACCGTCGTGCGGCAAAACCTGCTGTGGGCCGCCGTCTACAACGCGGTC
TGCGTGCCGCTGGCCGTGCTCGGATTCATGCCACCCTGGCTGGCCGGGTTGGGCATGGCGGCCAGTTCATTGCTGGTGGT
GCTGAATTCGGCACGGCTGGCGCGTATTCCCGGAGCCGCTTGA

Upstream 100 bases:

>100_bases
TCCTTTTTTTTGGGTAGCGCCTTCAGCTTAGGCGCACGGGTCCGGTTCCGGATTGATCCAGATCAACGGGACGGTTCGAT
GCGCTGGTTAACCTTGCCCC

Downstream 100 bases:

>100_bases
ATGGACATCCTCTTCCTGCTGATTCCGCTGTCGGTCGTGCTGGCGTTGCTCATACTCGGCGCGCTGGGCTGGGCTGTCTG
GCGTGGCCAGTTCGAGGCGC

Product: heavy metal translocating P-type ATPase

Products: ADP; Orthophosphate. [C]

Alternate protein names: E1-E2 type cation ATPase FixI [H]

Number of amino acids: Translated: 760; Mature: 760

Protein sequence:

>760_residues
MQTATLALGTEPVAPINAAWAALDERAEWEGFSRPVAGRDGWWETYLAIDGMHCAACSLTVEEALGQLPDVDGVQVNGAT
AMARLVWRPGRTRPSAWMAALQRAGYTALPAGDMLLAAPRLQAQRLMLWRWLVAGFCMMQVMMYAVPAYVAGPGEITPDV
QALLRWASWVLTLPVVLFSCWPFFATALRDLRGRRIGMDVPVALGILITFGASTAATFDASGPWGAEVWYDSLTMFVFFL
LSGRLLEQRLRDRTAGSLEALMRRLPDSVERQGADGQYERVALRRLVVGDLIRVLPGEVIPADGTVTAGDSRVDEALLTG
ESTPLQRCAGGAVIAGSHNLSGLLLVRVERVGAETRYASIVALMEQASVQKPRLAQVADRIANPFLLGVLLASAAAALWW
WPVDPAHAISVAVAVLIVTCPCALSLATPAATLAAAGALARRGVLVRRLQALESGASVDTVVFDKTGTLTEDRMAVLMRR
VRPGTDPTQALHLAAALAQHSHHPVSRAIVQAVAGAVPGAENITEIAGHGLQGQIAGGAAGRVLRLGSAAFCGAPLQDTP
AHGPQVHLADERGWLASFEFEEALRPDARPAVGRLQAMGLRVLLLSGDQGGAVLRLAARAGIDEAYGQRTPEDKLAHVRS
LQLRGRRVAMVGDGMNDGPVLACADLSVAMGHAVPLAQAKSDFVVLGSRLSDVPALLLQARRTRTVVRQNLLWAAVYNAV
CVPLAVLGFMPPWLAGLGMAASSLLVVLNSARLARIPGAA

Sequences:

>Translated_760_residues
MQTATLALGTEPVAPINAAWAALDERAEWEGFSRPVAGRDGWWETYLAIDGMHCAACSLTVEEALGQLPDVDGVQVNGAT
AMARLVWRPGRTRPSAWMAALQRAGYTALPAGDMLLAAPRLQAQRLMLWRWLVAGFCMMQVMMYAVPAYVAGPGEITPDV
QALLRWASWVLTLPVVLFSCWPFFATALRDLRGRRIGMDVPVALGILITFGASTAATFDASGPWGAEVWYDSLTMFVFFL
LSGRLLEQRLRDRTAGSLEALMRRLPDSVERQGADGQYERVALRRLVVGDLIRVLPGEVIPADGTVTAGDSRVDEALLTG
ESTPLQRCAGGAVIAGSHNLSGLLLVRVERVGAETRYASIVALMEQASVQKPRLAQVADRIANPFLLGVLLASAAAALWW
WPVDPAHAISVAVAVLIVTCPCALSLATPAATLAAAGALARRGVLVRRLQALESGASVDTVVFDKTGTLTEDRMAVLMRR
VRPGTDPTQALHLAAALAQHSHHPVSRAIVQAVAGAVPGAENITEIAGHGLQGQIAGGAAGRVLRLGSAAFCGAPLQDTP
AHGPQVHLADERGWLASFEFEEALRPDARPAVGRLQAMGLRVLLLSGDQGGAVLRLAARAGIDEAYGQRTPEDKLAHVRS
LQLRGRRVAMVGDGMNDGPVLACADLSVAMGHAVPLAQAKSDFVVLGSRLSDVPALLLQARRTRTVVRQNLLWAAVYNAV
CVPLAVLGFMPPWLAGLGMAASSLLVVLNSARLARIPGAA
>Mature_760_residues
MQTATLALGTEPVAPINAAWAALDERAEWEGFSRPVAGRDGWWETYLAIDGMHCAACSLTVEEALGQLPDVDGVQVNGAT
AMARLVWRPGRTRPSAWMAALQRAGYTALPAGDMLLAAPRLQAQRLMLWRWLVAGFCMMQVMMYAVPAYVAGPGEITPDV
QALLRWASWVLTLPVVLFSCWPFFATALRDLRGRRIGMDVPVALGILITFGASTAATFDASGPWGAEVWYDSLTMFVFFL
LSGRLLEQRLRDRTAGSLEALMRRLPDSVERQGADGQYERVALRRLVVGDLIRVLPGEVIPADGTVTAGDSRVDEALLTG
ESTPLQRCAGGAVIAGSHNLSGLLLVRVERVGAETRYASIVALMEQASVQKPRLAQVADRIANPFLLGVLLASAAAALWW
WPVDPAHAISVAVAVLIVTCPCALSLATPAATLAAAGALARRGVLVRRLQALESGASVDTVVFDKTGTLTEDRMAVLMRR
VRPGTDPTQALHLAAALAQHSHHPVSRAIVQAVAGAVPGAENITEIAGHGLQGQIAGGAAGRVLRLGSAAFCGAPLQDTP
AHGPQVHLADERGWLASFEFEEALRPDARPAVGRLQAMGLRVLLLSGDQGGAVLRLAARAGIDEAYGQRTPEDKLAHVRS
LQLRGRRVAMVGDGMNDGPVLACADLSVAMGHAVPLAQAKSDFVVLGSRLSDVPALLLQARRTRTVVRQNLLWAAVYNAV
CVPLAVLGFMPPWLAGLGMAASSLLVVLNSARLARIPGAA

Specific function: FixI is a pump of a specific cation involved in symbiotic nitrogen fixation. The four proteins FixG, FixH, FixI, and FixS may participate in a membrane-bound complex coupling the FixI cation pump with a redox process catalyzed by FixG [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HMA domain [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=514, Percent_Identity=28.4046692607004, Blast_Score=187, Evalue=4e-47,
Organism=Homo sapiens, GI115529486, Length=539, Percent_Identity=28.2003710575139, Blast_Score=179, Evalue=1e-44,
Organism=Homo sapiens, GI55743073, Length=500, Percent_Identity=25.8, Blast_Score=139, Evalue=1e-32,
Organism=Homo sapiens, GI153946397, Length=219, Percent_Identity=28.7671232876712, Blast_Score=68, Evalue=3e-11,
Organism=Escherichia coli, GI1786691, Length=759, Percent_Identity=32.6745718050066, Blast_Score=296, Evalue=4e-81,
Organism=Escherichia coli, GI1789879, Length=735, Percent_Identity=29.5238095238095, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1786914, Length=446, Percent_Identity=28.47533632287, Blast_Score=122, Evalue=9e-29,
Organism=Escherichia coli, GI2367363, Length=573, Percent_Identity=23.2111692844677, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556548, Length=519, Percent_Identity=28.5163776493256, Blast_Score=159, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6319772, Length=636, Percent_Identity=29.7169811320755, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6320475, Length=562, Percent_Identity=30.0711743772242, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6325221, Length=584, Percent_Identity=22.6027397260274, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321430, Length=561, Percent_Identity=23.1729055258467, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI221329854, Length=520, Percent_Identity=29.8076923076923, Blast_Score=186, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR006403
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: 3.6.3.4 [C]

Molecular weight: Translated: 80574; Mature: 80574

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00154 ATPASE_E1_E2 ; PS50846 HMA_2 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTATLALGTEPVAPINAAWAALDERAEWEGFSRPVAGRDGWWETYLAIDGMHCAACSLT
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHEECCCHHHHHHEE
VEEALGQLPDVDGVQVNGATAMARLVWRPGRTRPSAWMAALQRAGYTALPAGDMLLAAPR
HHHHHHCCCCCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCH
LQAQRLMLWRWLVAGFCMMQVMMYAVPAYVAGPGEITPDVQALLRWASWVLTLPVVLFSC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WPFFATALRDLRGRRIGMDVPVALGILITFGASTAATFDASGPWGAEVWYDSLTMFVFFL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
LSGRLLEQRLRDRTAGSLEALMRRLPDSVERQGADGQYERVALRRLVVGDLIRVLPGEVI
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
PADGTVTAGDSRVDEALLTGESTPLQRCAGGAVIAGSHNLSGLLLVRVERVGAETRYASI
CCCCCEECCCHHHHHHHHCCCCCHHHHHCCCEEEECCCCCCEEEEEEEHHHCCHHHHHHH
VALMEQASVQKPRLAQVADRIANPFLLGVLLASAAAALWWWPVDPAHAISVAVAVLIVTC
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHH
PCALSLATPAATLAAAGALARRGVLVRRLQALESGASVDTVVFDKTGTLTEDRMAVLMRR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
VRPGTDPTQALHLAAALAQHSHHPVSRAIVQAVAGAVPGAENITEIAGHGLQGQIAGGAA
HCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCC
GRVLRLGSAAFCGAPLQDTPAHGPQVHLADERGWLASFEFEEALRPDARPAVGRLQAMGL
CHHHEECCHHHCCCCCCCCCCCCCEEEEECCCCCEECCCHHHHCCCCCCHHHHHHHHCCE
RVLLLSGDQGGAVLRLAARAGIDEAYGQRTPEDKLAHVRSLQLRGRRVAMVGDGMNDGPV
EEEEEECCCCCCEEEEHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCE
LACADLSVAMGHAVPLAQAKSDFVVLGSRLSDVPALLLQARRTRTVVRQNLLWAAVYNAV
EEEECHHHHHCCCCCHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVPLAVLGFMPPWLAGLGMAASSLLVVLNSARLARIPGAA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure
MQTATLALGTEPVAPINAAWAALDERAEWEGFSRPVAGRDGWWETYLAIDGMHCAACSLT
CCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHEECCCHHHHHHEE
VEEALGQLPDVDGVQVNGATAMARLVWRPGRTRPSAWMAALQRAGYTALPAGDMLLAAPR
HHHHHHCCCCCCCEEECCHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCH
LQAQRLMLWRWLVAGFCMMQVMMYAVPAYVAGPGEITPDVQALLRWASWVLTLPVVLFSC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
WPFFATALRDLRGRRIGMDVPVALGILITFGASTAATFDASGPWGAEVWYDSLTMFVFFL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
LSGRLLEQRLRDRTAGSLEALMRRLPDSVERQGADGQYERVALRRLVVGDLIRVLPGEVI
HHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEE
PADGTVTAGDSRVDEALLTGESTPLQRCAGGAVIAGSHNLSGLLLVRVERVGAETRYASI
CCCCCEECCCHHHHHHHHCCCCCHHHHHCCCEEEECCCCCCEEEEEEEHHHCCHHHHHHH
VALMEQASVQKPRLAQVADRIANPFLLGVLLASAAAALWWWPVDPAHAISVAVAVLIVTC
HHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHH
PCALSLATPAATLAAAGALARRGVLVRRLQALESGASVDTVVFDKTGTLTEDRMAVLMRR
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHH
VRPGTDPTQALHLAAALAQHSHHPVSRAIVQAVAGAVPGAENITEIAGHGLQGQIAGGAA
HCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCC
GRVLRLGSAAFCGAPLQDTPAHGPQVHLADERGWLASFEFEEALRPDARPAVGRLQAMGL
CHHHEECCHHHCCCCCCCCCCCCCEEEEECCCCCEECCCHHHHCCCCCCHHHHHHHHCCE
RVLLLSGDQGGAVLRLAARAGIDEAYGQRTPEDKLAHVRSLQLRGRRVAMVGDGMNDGPV
EEEEEECCCCCCEEEEHHHCCCHHHHCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCE
LACADLSVAMGHAVPLAQAKSDFVVLGSRLSDVPALLLQARRTRTVVRQNLLWAAVYNAV
EEEECHHHHHCCCCCHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CVPLAVLGFMPPWLAGLGMAASSLLVVLNSARLARIPGAA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Cu [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O [C]

Specific reaction: ATP + H2O = ADP + Orthophosphate. [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA