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The map label for this gene is rhtB [H]

Identifier: 91790082

GI number: 91790082

Start: 4470480

End: 4471118

Strand: Direct

Name: rhtB [H]

Synonym: Bpro_4244

Alternate gene names: 91790082

Gene position: 4470480-4471118 (Clockwise)

Preceding gene: 91790081

Following gene: 91790083

Centisome position: 85.97

GC content: 62.91

Gene sequence:

>639_bases
ATGTTGACAATTCAGGAACTCGCCTGGTTTGCACTTGCTGCGCTCGTGCTGGTGCTCACGCCCGGGCCGAACATGATTTA
TTGCATTTCGCGCACCCTGTGTCAGGGCCGCAGTGCCGGGCTCATTTCGCTGGGCGGCGTCGTGCTGGGTTTTGTGGTCC
ACCTGCTGGCCGCTTCATTCGGCCTGACGGCCCTGCTGCTGGCCGTTCCGCTGGCCTTTGACACCATCAAGCTGGCCGGC
GCGATGTATCTGCTGTGGCTGGCCTGGCAGGCGGTCAAGCCGGGTGGATTGGCGCCGTTCGAGACGCACGAATTGCCGCA
CGATCCGCCGCACAGGCTGTTCCTCATGGGCTTCATGACCAATCTGCTCAACCCGAAGGTGGCCATGTTTTACCTGTCTT
TCTTCCCGCAGTTTCTGCATCCGGAACGCGGCGAACTGCTGCTGCAAAGCCTGAGCCTGGGCACCGTGCAAATCGGCATC
AGCACCGGCGTCAACACGGTGCTCGTCTGCTTCGCGGCCGGGATGGCCGGCTTCCTGAACCGCAACCGCCGCTGGATGCG
CGTGCAGCGTTATGTGATGGGTAGCGTGCTGGGCTTGCTGGCGCTACGCCTGCTGTCCGAGAAGCGGGCTGCGGCATGA

Upstream 100 bases:

>100_bases
TCTCATCGCCACGCTGCAAACGCCGCGGGGTGTCGTCACGCTGGAGTCCAGGGGTATTTAAAGCGTCGCAAGAGTAAAGA
GAAAGAGAAAAAGGAACACC

Downstream 100 bases:

>100_bases
GCCGCCGGGCCGCTCCCAAGGCGAAAGGCACCGCAGCGCGCAGTGCGGAGGTCAAGCAATGAGCACCACGCTGCACGGCA
CGCCCACGGTCACCCCGCGG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MLTIQELAWFALAALVLVLTPGPNMIYCISRTLCQGRSAGLISLGGVVLGFVVHLLAASFGLTALLLAVPLAFDTIKLAG
AMYLLWLAWQAVKPGGLAPFETHELPHDPPHRLFLMGFMTNLLNPKVAMFYLSFFPQFLHPERGELLLQSLSLGTVQIGI
STGVNTVLVCFAAGMAGFLNRNRRWMRVQRYVMGSVLGLLALRLLSEKRAAA

Sequences:

>Translated_212_residues
MLTIQELAWFALAALVLVLTPGPNMIYCISRTLCQGRSAGLISLGGVVLGFVVHLLAASFGLTALLLAVPLAFDTIKLAG
AMYLLWLAWQAVKPGGLAPFETHELPHDPPHRLFLMGFMTNLLNPKVAMFYLSFFPQFLHPERGELLLQSLSLGTVQIGI
STGVNTVLVCFAAGMAGFLNRNRRWMRVQRYVMGSVLGLLALRLLSEKRAAA
>Mature_212_residues
MLTIQELAWFALAALVLVLTPGPNMIYCISRTLCQGRSAGLISLGGVVLGFVVHLLAASFGLTALLLAVPLAFDTIKLAG
AMYLLWLAWQAVKPGGLAPFETHELPHDPPHRLFLMGFMTNLLNPKVAMFYLSFFPQFLHPERGELLLQSLSLGTVQIGI
STGVNTVLVCFAAGMAGFLNRNRRWMRVQRYVMGSVLGLLALRLLSEKRAAA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=204, Percent_Identity=28.921568627451, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1786522, Length=194, Percent_Identity=27.8350515463918, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788099, Length=136, Percent_Identity=30.1470588235294, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23122; Mature: 23122

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTIQELAWFALAALVLVLTPGPNMIYCISRTLCQGRSAGLISLGGVVLGFVVHLLAASF
CCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GLTALLLAVPLAFDTIKLAGAMYLLWLAWQAVKPGGLAPFETHELPHDPPHRLFLMGFMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
NLLNPKVAMFYLSFFPQFLHPERGELLLQSLSLGTVQIGISTGVNTVLVCFAAGMAGFLN
HHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEHHHCHHHHHHHHHHHHHHHHH
RNRRWMRVQRYVMGSVLGLLALRLLSEKRAAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTIQELAWFALAALVLVLTPGPNMIYCISRTLCQGRSAGLISLGGVVLGFVVHLLAASF
CCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
GLTALLLAVPLAFDTIKLAGAMYLLWLAWQAVKPGGLAPFETHELPHDPPHRLFLMGFMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
NLLNPKVAMFYLSFFPQFLHPERGELLLQSLSLGTVQIGISTGVNTVLVCFAAGMAGFLN
HHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEHHHCHHHHHHHHHHHHHHHHH
RNRRWMRVQRYVMGSVLGLLALRLLSEKRAAA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]