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The map label for this gene is crp [H]

Identifier: 91790057

GI number: 91790057

Start: 4439705

End: 4440364

Strand: Reverse

Name: crp [H]

Synonym: Bpro_4219

Alternate gene names: 91790057

Gene position: 4440364-4439705 (Counterclockwise)

Preceding gene: 91790058

Following gene: 91790050

Centisome position: 85.39

GC content: 62.88

Gene sequence:

>660_bases
ATGAGTTCATTTACCGCCTCAGGCGAGGACCTGTACGCCCGTTTGCCCCCCGAATTGCGTGCGCTGGCCGTGCGCGGCAT
GGTGCGCAGCTACCCCAAAAAGGCGGTGGTGATCAACGAAGCCGAAATTGGCGACAGCCTGTTTGTGCTGCTCAAGGGCA
GCGTCAAGGTGTTTTCGATGGACGAAAGCGGCCGGGAGATCACTTTCGGCAGGATTCATGCGGGTGACTATTTTGGTGAA
ATGTCGCTCGATGGCGGGCCCCGCTCGGCGTCGGTGATCACCGTGGAGCCCTGCACCTGTGCCGTCGTCAACCGCACCGA
AGTGGGTGAGCACCTGGCCAGCGCGCCCGAGTTTGCCCTTAATCTGGTGATGCGGGTCATCCGCCGCGCGCGCGACGCCA
CGGAGGCTGCGCGCAACATGGCGCTGCTGGACGTGTATGGCCGGCTCATCGCGGTGCTTGAAAAGCAGGATGGCCTGACA
ACCAGCACCACGGCTGCCGGCGTCACGCTCGAAGCCATCACGCACCAGGAAATCGCCAGCCGCGTCGGCGCCTCCCGCGA
GATGGTCAGCCGCCTGCTCAAAGACCTGGAAAAGGGCGGTTACATCGAGATGGAAACCAAGCGCATTACGCTGCTCAAGA
AGCTGCCCCTGCGCTGGTAG

Upstream 100 bases:

>100_bases
TTGCGAACTCCGCAAAAGACCGGTTTTGCCGGCGCCAGCATGCGCTTTTGAACGCTCAGGCCCGTCTTTTTGACGCTAAC
GTGCTAGATTACGCCTCATC

Downstream 100 bases:

>100_bases
TTATTCCCCCGGCATTTACCTCGCCCCCGCACCGCTGACCGGGCTGCCGCCCAGCTCCGGCATAGGTGCAATTTAAAGCG
GCAGCAAGCCGCTACCCTCG

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSSFTASGEDLYARLPPELRALAVRGMVRSYPKKAVVINEAEIGDSLFVLLKGSVKVFSMDESGREITFGRIHAGDYFGE
MSLDGGPRSASVITVEPCTCAVVNRTEVGEHLASAPEFALNLVMRVIRRARDATEAARNMALLDVYGRLIAVLEKQDGLT
TSTTAAGVTLEAITHQEIASRVGASREMVSRLLKDLEKGGYIEMETKRITLLKKLPLRW

Sequences:

>Translated_219_residues
MSSFTASGEDLYARLPPELRALAVRGMVRSYPKKAVVINEAEIGDSLFVLLKGSVKVFSMDESGREITFGRIHAGDYFGE
MSLDGGPRSASVITVEPCTCAVVNRTEVGEHLASAPEFALNLVMRVIRRARDATEAARNMALLDVYGRLIAVLEKQDGLT
TSTTAAGVTLEAITHQEIASRVGASREMVSRLLKDLEKGGYIEMETKRITLLKKLPLRW
>Mature_218_residues
SSFTASGEDLYARLPPELRALAVRGMVRSYPKKAVVINEAEIGDSLFVLLKGSVKVFSMDESGREITFGRIHAGDYFGEM
SLDGGPRSASVITVEPCTCAVVNRTEVGEHLASAPEFALNLVMRVIRRARDATEAARNMALLDVYGRLIAVLEKQDGLTT
STTAAGVTLEAITHQEIASRVGASREMVSRLLKDLEKGGYIEMETKRITLLKKLPLRW

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=201, Percent_Identity=31.8407960199005, Blast_Score=99, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 23959; Mature: 23828

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSFTASGEDLYARLPPELRALAVRGMVRSYPKKAVVINEAEIGDSLFVLLKGSVKVFSM
CCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCEEEEEE
DESGREITFGRIHAGDYFGEMSLDGGPRSASVITVEPCTCAVVNRTEVGEHLASAPEFAL
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEECHHHHHHHHHCCHHHHH
NLVMRVIRRARDATEAARNMALLDVYGRLIAVLEKQDGLTTSTTAAGVTLEAITHQEIAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCEEHHHHHHHHHHH
RVGASREMVSRLLKDLEKGGYIEMETKRITLLKKLPLRW
HHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSFTASGEDLYARLPPELRALAVRGMVRSYPKKAVVINEAEIGDSLFVLLKGSVKVFSM
CCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEECCEEEEEE
DESGREITFGRIHAGDYFGEMSLDGGPRSASVITVEPCTCAVVNRTEVGEHLASAPEFAL
CCCCCEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEECHHHHHHHHHCCHHHHH
NLVMRVIRRARDATEAARNMALLDVYGRLIAVLEKQDGLTTSTTAAGVTLEAITHQEIAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCEEHHHHHHHHHHH
RVGASREMVSRLLKDLEKGGYIEMETKRITLLKKLPLRW
HHCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]