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The map label for this gene is livK [H]

Identifier: 91789978

GI number: 91789978

Start: 4352091

End: 4353203

Strand: Direct

Name: livK [H]

Synonym: Bpro_4140

Alternate gene names: 91789978

Gene position: 4352091-4353203 (Clockwise)

Preceding gene: 91789975

Following gene: 91789981

Centisome position: 83.69

GC content: 61.1

Gene sequence:

>1113_bases
ATGAAAAAACTGAATTTATGGTGCGGCGCCATCGTGCTGATGGCCTTGGGTCACGCCAGCCAGGCGCAAATCCTGATCGG
ACAGACCGCCGGGTTTACCGGCGCGGTGGGCGCAGGTGTCAAGGAAACCACAGAAGGCGCCAAGCTTTACATTGACACCA
TCAACGCCAAGGGCGGTGTCAACGGCCAGAAGATTGAGCTGGTCTCGCTCGACGACAAGTTCGACCCCAAGCTGGCGGCC
GAAAACGCGCGCAAGCTGATAGAAGAGCAGAACGTGGTGGCAATGTTCCTGACGCGCGGCACGCCCCACACGGAAGGCAT
CATTCCCATCCTTGAAAAAAATGGCGTGCCGCTGATAGGCCCGTCCACCGGTGCCATGGTGCTGCACCAGCCCGTCAAGA
AGCATGTCTTCAATGTGCGCGCCACCTACCAGCGCGAAGCCGAAAAGGCCATCACCCATCTGCATTCCATGGGCATCACG
CGGATCGGCATTGTCTACGCGGACGACAGCTTCGGCGCCGATGGCGTGGCCGGCGCGCAAAAAGGCCTGACGACGGCCAA
GCTGCAGCCCGTTGTGCTGGAAAAATTCAACCGCGCCAAGCCCGACTTCGGGCCGATTGCTCCCAAGGTTTTTCAGTCCA
ATGCGCAGGCCATCATGATGGTGGCCTCCGGCCAGGCGGTGGTGGATGGCGTCAAGGCCTTCCGCGCGGCGGGCTCTGCG
GCACAGATTGTGACGCTGTCCAACAATGCCTCCAGCGGTTTTGTGAAGAGCCTGGGCGAGAACGCGCGGGGCGTGATCGT
CACGCAGGTCTTTCCCTACGAGCGCTCCATTGCCTTCCCCATGGTCAGGGAAGCCCAGGAAATGGCCAAGGCGAAGGGAC
AGGGCGAAATCACTCCGGCCATGCTGGAGGGCTTTGCCGCGGCCAAGGTGCTGGTGGAAGGCCTGCGCCGGGCCGGCGCC
AAACCCACCCGCGAAAAAATCCAGGCAGCCCTGGAAGGTCTTCGCAAACTGGATATTGGCGGACTGGAAGTGAACTACAG
CGCAGAGGACCACACCGGGCTTGATTTCGCCGATCTGTCCATCATCGGGACCGACGGGAAATTCAAGCGCTGA

Upstream 100 bases:

>100_bases
GCTCCTGCCCATGATTTCCTGTTCGTGGTTTCCTGTTCGTGATTTCCCCACTTCCTTATTCAGCGGGGATGCGTAAACTG
AACTCCACCAGGAGCAAAAC

Downstream 100 bases:

>100_bases
GGTGAACGCCCCGGCATCATGTCGAGACGGGGCCCTGTCATGCCCGGAGCACGGTAGCGCCACTCGCCGTCGCGGCCTCA
GTCATACAGGGCGCGCTCCA

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: L-BP; LS-BP [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKKLNLWCGAIVLMALGHASQAQILIGQTAGFTGAVGAGVKETTEGAKLYIDTINAKGGVNGQKIELVSLDDKFDPKLAA
ENARKLIEEQNVVAMFLTRGTPHTEGIIPILEKNGVPLIGPSTGAMVLHQPVKKHVFNVRATYQREAEKAITHLHSMGIT
RIGIVYADDSFGADGVAGAQKGLTTAKLQPVVLEKFNRAKPDFGPIAPKVFQSNAQAIMMVASGQAVVDGVKAFRAAGSA
AQIVTLSNNASSGFVKSLGENARGVIVTQVFPYERSIAFPMVREAQEMAKAKGQGEITPAMLEGFAAAKVLVEGLRRAGA
KPTREKIQAALEGLRKLDIGGLEVNYSAEDHTGLDFADLSIIGTDGKFKR

Sequences:

>Translated_370_residues
MKKLNLWCGAIVLMALGHASQAQILIGQTAGFTGAVGAGVKETTEGAKLYIDTINAKGGVNGQKIELVSLDDKFDPKLAA
ENARKLIEEQNVVAMFLTRGTPHTEGIIPILEKNGVPLIGPSTGAMVLHQPVKKHVFNVRATYQREAEKAITHLHSMGIT
RIGIVYADDSFGADGVAGAQKGLTTAKLQPVVLEKFNRAKPDFGPIAPKVFQSNAQAIMMVASGQAVVDGVKAFRAAGSA
AQIVTLSNNASSGFVKSLGENARGVIVTQVFPYERSIAFPMVREAQEMAKAKGQGEITPAMLEGFAAAKVLVEGLRRAGA
KPTREKIQAALEGLRKLDIGGLEVNYSAEDHTGLDFADLSIIGTDGKFKR
>Mature_370_residues
MKKLNLWCGAIVLMALGHASQAQILIGQTAGFTGAVGAGVKETTEGAKLYIDTINAKGGVNGQKIELVSLDDKFDPKLAA
ENARKLIEEQNVVAMFLTRGTPHTEGIIPILEKNGVPLIGPSTGAMVLHQPVKKHVFNVRATYQREAEKAITHLHSMGIT
RIGIVYADDSFGADGVAGAQKGLTTAKLQPVVLEKFNRAKPDFGPIAPKVFQSNAQAIMMVASGQAVVDGVKAFRAAGSA
AQIVTLSNNASSGFVKSLGENARGVIVTQVFPYERSIAFPMVREAQEMAKAKGQGEITPAMLEGFAAAKVLVEGLRRAGA
KPTREKIQAALEGLRKLDIGGLEVNYSAEDHTGLDFADLSIIGTDGKFKR

Specific function: This protein is a component of the leucine-specific transport system, which is one of the two periplasmic binding protein-dependent transport systems of the high-affinity transport of the branched-chain amino acids in E.coli [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=315, Percent_Identity=22.2222222222222, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI48994941, Length=317, Percent_Identity=23.3438485804416, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39186; Mature: 39186

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLNLWCGAIVLMALGHASQAQILIGQTAGFTGAVGAGVKETTEGAKLYIDTINAKGGV
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
NGQKIELVSLDDKFDPKLAAENARKLIEEQNVVAMFLTRGTPHTEGIIPILEKNGVPLIG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEECCCCEEEC
PSTGAMVLHQPVKKHVFNVRATYQREAEKAITHLHSMGITRIGIVYADDSFGADGVAGAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHH
KGLTTAKLQPVVLEKFNRAKPDFGPIAPKVFQSNAQAIMMVASGQAVVDGVKAFRAAGSA
CCCCHHHCCHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCC
AQIVTLSNNASSGFVKSLGENARGVIVTQVFPYERSIAFPMVREAQEMAKAKGQGEITPA
EEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHH
MLEGFAAAKVLVEGLRRAGAKPTREKIQAALEGLRKLDIGGLEVNYSAEDHTGLDFADLS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
IIGTDGKFKR
EEECCCCCCC
>Mature Secondary Structure
MKKLNLWCGAIVLMALGHASQAQILIGQTAGFTGAVGAGVKETTEGAKLYIDTINAKGGV
CCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCC
NGQKIELVSLDDKFDPKLAAENARKLIEEQNVVAMFLTRGTPHTEGIIPILEKNGVPLIG
CCCEEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCEEEEECCCCEEEC
PSTGAMVLHQPVKKHVFNVRATYQREAEKAITHLHSMGITRIGIVYADDSFGADGVAGAQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHH
KGLTTAKLQPVVLEKFNRAKPDFGPIAPKVFQSNAQAIMMVASGQAVVDGVKAFRAAGSA
CCCCHHHCCHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCCC
AQIVTLSNNASSGFVKSLGENARGVIVTQVFPYERSIAFPMVREAQEMAKAKGQGEITPA
EEEEEECCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCHH
MLEGFAAAKVLVEGLRRAGAKPTREKIQAALEGLRKLDIGGLEVNYSAEDHTGLDFADLS
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCEEEEE
IIGTDGKFKR
EEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3891753; 4077929; 2195019; 8041620; 9278503; 6990419; 9298646; 2649683 [H]