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The map label for this gene is fixJ [H]

Identifier: 91789940

GI number: 91789940

Start: 4309556

End: 4310245

Strand: Direct

Name: fixJ [H]

Synonym: Bpro_4101

Alternate gene names: 91789940

Gene position: 4309556-4310245 (Clockwise)

Preceding gene: 91789939

Following gene: 91789941

Centisome position: 82.87

GC content: 67.39

Gene sequence:

>690_bases
ATGAAAGAACGCTCCGGCAACGCACCCCGGGGCGCGGCGGGACCCCATGAGGAAAAAACCGCGGGGGCCCTGCTGTCACC
GCTGATCCACCTGATCGACGACGACGAGGCGGTGCGCAGCAGCCTGGCGCTGCTGATCAGCACGGTCGGCCTGCGGGTGC
AGAGCTGGAGCGACCCGCAGGCCTTCATCAGCGGCTTTGACCGCCAGGGCATTGGCGCCATCGTGCTGGACGTGCGCATG
CCCGGCATCAGCGGTCTGACCATACTGGACACGCTGATGGCCCAGGGCGTGGACCAGCCGGTGATCATGCTGACCGGGCA
CGGCACGGTGGACATGTGCCGCCGCGCCTTCAAGTCCGGCGCGGCCGAATTCCTGGAAAAGCCCGTCGATGACGAGCAAC
TGCTGGAGGCATTGCAAAACGCGGTCCGCCAGCATGTGAAGTCGCGCGAGCGGCGCCAGACCGACCAGCAGGCGCGGCAA
CGCTATGCGCAGCTGTCGGAGCGCGAACGCGAGGTGCTGGGCCTGATCGTCGCCGGCCTGACCAACAAGGAGATCGGCCG
GGCGCTGGCTGTCTCGCCGCGCACCGTGGAAACCCACCGCGCCAACCTGTTTGCCAAGCTGGAGGCCGAGTCATTGGCGC
AGCTGATCCGGCAGTATGCGACGCTGGCAGACGAAGCCGACGGTCGATAG

Upstream 100 bases:

>100_bases
GCGAAACACTGGCCACCGGCATGGGCGGCAGCCTGACGGCGGCACACAACCTGCCGCGCGGCGCGGTATTCAGGCTGTCC
TTACCCCTGGCAGACACACC

Downstream 100 bases:

>100_bases
GTATTCACCGAAAGCCAGTCCAAGCCGCGGAACCGGCTTCGCCGGGCCGCAGGCGGGCGGCCCCCTCGGGGGGCAGGGAG
TTACGCGAAGCGAAGGACCG

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKERSGNAPRGAAGPHEEKTAGALLSPLIHLIDDDEAVRSSLALLISTVGLRVQSWSDPQAFISGFDRQGIGAIVLDVRM
PGISGLTILDTLMAQGVDQPVIMLTGHGTVDMCRRAFKSGAAEFLEKPVDDEQLLEALQNAVRQHVKSRERRQTDQQARQ
RYAQLSEREREVLGLIVAGLTNKEIGRALAVSPRTVETHRANLFAKLEAESLAQLIRQYATLADEADGR

Sequences:

>Translated_229_residues
MKERSGNAPRGAAGPHEEKTAGALLSPLIHLIDDDEAVRSSLALLISTVGLRVQSWSDPQAFISGFDRQGIGAIVLDVRM
PGISGLTILDTLMAQGVDQPVIMLTGHGTVDMCRRAFKSGAAEFLEKPVDDEQLLEALQNAVRQHVKSRERRQTDQQARQ
RYAQLSEREREVLGLIVAGLTNKEIGRALAVSPRTVETHRANLFAKLEAESLAQLIRQYATLADEADGR
>Mature_229_residues
MKERSGNAPRGAAGPHEEKTAGALLSPLIHLIDDDEAVRSSLALLISTVGLRVQSWSDPQAFISGFDRQGIGAIVLDVRM
PGISGLTILDTLMAQGVDQPVIMLTGHGTVDMCRRAFKSGAAEFLEKPVDDEQLLEALQNAVRQHVKSRERRQTDQQARQ
RYAQLSEREREVLGLIVAGLTNKEIGRALAVSPRTVETHRANLFAKLEAESLAQLIRQYATLADEADGR

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=195, Percent_Identity=31.2820512820513, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1788521, Length=154, Percent_Identity=34.4155844155844, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1788222, Length=187, Percent_Identity=26.2032085561497, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786747, Length=164, Percent_Identity=28.0487804878049, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790299, Length=127, Percent_Identity=23.6220472440945, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 25028; Mature: 25028

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERSGNAPRGAAGPHEEKTAGALLSPLIHLIDDDEAVRSSLALLISTVGLRVQSWSDPQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHH
AFISGFDRQGIGAIVLDVRMPGISGLTILDTLMAQGVDQPVIMLTGHGTVDMCRRAFKSG
HHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
AAEFLEKPVDDEQLLEALQNAVRQHVKSRERRQTDQQARQRYAQLSEREREVLGLIVAGL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNKEIGRALAVSPRTVETHRANLFAKLEAESLAQLIRQYATLADEADGR
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKERSGNAPRGAAGPHEEKTAGALLSPLIHLIDDDEAVRSSLALLISTVGLRVQSWSDPQ
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHH
AFISGFDRQGIGAIVLDVRMPGISGLTILDTLMAQGVDQPVIMLTGHGTVDMCRRAFKSG
HHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHH
AAEFLEKPVDDEQLLEALQNAVRQHVKSRERRQTDQQARQRYAQLSEREREVLGLIVAGL
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNKEIGRALAVSPRTVETHRANLFAKLEAESLAQLIRQYATLADEADGR
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2046550 [H]