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The map label for this gene is rfbB [H]

Identifier: 91789862

GI number: 91789862

Start: 4220109

End: 4221191

Strand: Reverse

Name: rfbB [H]

Synonym: Bpro_4022

Alternate gene names: 91789862

Gene position: 4221191-4220109 (Counterclockwise)

Preceding gene: 91789863

Following gene: 91789861

Centisome position: 81.17

GC content: 60.94

Gene sequence:

>1083_bases
ATGATTTTCGTTACCGGAGGCGCCGGCTTTATTGGTGCCAACTTTGTTCTTGACTGGCTTGCGCAAACCGATGAGCCTGT
GGTCAATATCGACAATTTGACCTACGCGGGCAACCTTGAAAACCTCTCCTCCCTGCAGGCTGACCCGCGCCATGTCTTTG
TCAAGGGCGACATCGGCGATTCCGCGTTGGTCGGCCGCCTGCTGGCCACCCACCAGCCCAGAGCCATCCTCAACTTTGCG
GCCGAGTCACATGTAGACCGTTCCATTCACGGCCCGGAAGACTTCATTCAGACCAATATCGTCGGCACGTTCCGCCTGCT
GGAGGCCGTCAGGGCGTATTGGGGCGGCTTGCAAGGCGATGCCAAGGCGGAGTTCCGCTTTTTGCATGTCTCAACAGACG
AGGTCTATGGCTCGCTCGCGCCGGCCGACGCCGCCTTCACGGAAACGCACCGCTACGAGCCCAACAGCCCGTACTCGGCC
AGCAAGGCCGCCAGCGACCACCTGGTGCGCGCCTACCACCACACCTACGGCCTGCCGGTGCTCACCACCAACTGCAGCAA
CAACTACGGCCCCTACCATTTCCCCGAGAAGCTCATCCCCCTGATGATCGTCAACGCCCTGGCCGGCAAGCCCCTGCCGG
TGTACGGCGACGGCATGCAGGTGCGCGACTGGCTCTACGTGAAAGACCATTGCAGCGCCATTCGCCGTGTGCTGGAAGCC
GGCAAAACCGGCGAGGTCTACAACGTGGGCGGCTGGAACGAAAAGCCCAACATCGAGATCGTCAACATCGTGTGCACGCT
GCTTGACGAATTGCGTCCCCGCGCCGACGGCAAAAACTACCGCGAGCAAATCAGCTACGTCAAAGACCGCCCCGGCCACG
ACCGCCGCTACGCCATAGACGCCAGCAAAATCCAGCGCGAATTGGGCTGGAAACCGGCTGAAACCTTTGAGACCGGTATC
CGTAAAACCGTGCAGTGGTACCTGGCCAACCCCGACTGGGTGGCCCATGTGCAAAGCGGGGCCTACCGCGAGTGGACGGC
CAAACAGTACGAGTACGGACAAACCCAAAAGGCTGCAGCTTGA

Upstream 100 bases:

>100_bases
TCTATGTACAAACGAGGCTGGACGCTGCGGTGGGATAATTGCCGGGTCAAGGGTGACTGCCCTGCTGCGTCCTTAACAGT
GACCTCCATCAAAGGCTTGC

Downstream 100 bases:

>100_bases
AAATCCTGCTTTTCGGCAAAAACGGCCAGGTCGGCTGGGAACTACAGCGCAGCCTGGCTCCTTTGGGTGAACTCACGGCG
CTCGACCGTCACAGCACAGA

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MIFVTGGAGFIGANFVLDWLAQTDEPVVNIDNLTYAGNLENLSSLQADPRHVFVKGDIGDSALVGRLLATHQPRAILNFA
AESHVDRSIHGPEDFIQTNIVGTFRLLEAVRAYWGGLQGDAKAEFRFLHVSTDEVYGSLAPADAAFTETHRYEPNSPYSA
SKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMIVNALAGKPLPVYGDGMQVRDWLYVKDHCSAIRRVLEA
GKTGEVYNVGGWNEKPNIEIVNIVCTLLDELRPRADGKNYREQISYVKDRPGHDRRYAIDASKIQRELGWKPAETFETGI
RKTVQWYLANPDWVAHVQSGAYREWTAKQYEYGQTQKAAA

Sequences:

>Translated_360_residues
MIFVTGGAGFIGANFVLDWLAQTDEPVVNIDNLTYAGNLENLSSLQADPRHVFVKGDIGDSALVGRLLATHQPRAILNFA
AESHVDRSIHGPEDFIQTNIVGTFRLLEAVRAYWGGLQGDAKAEFRFLHVSTDEVYGSLAPADAAFTETHRYEPNSPYSA
SKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMIVNALAGKPLPVYGDGMQVRDWLYVKDHCSAIRRVLEA
GKTGEVYNVGGWNEKPNIEIVNIVCTLLDELRPRADGKNYREQISYVKDRPGHDRRYAIDASKIQRELGWKPAETFETGI
RKTVQWYLANPDWVAHVQSGAYREWTAKQYEYGQTQKAAA
>Mature_360_residues
MIFVTGGAGFIGANFVLDWLAQTDEPVVNIDNLTYAGNLENLSSLQADPRHVFVKGDIGDSALVGRLLATHQPRAILNFA
AESHVDRSIHGPEDFIQTNIVGTFRLLEAVRAYWGGLQGDAKAEFRFLHVSTDEVYGSLAPADAAFTETHRYEPNSPYSA
SKAASDHLVRAYHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMIVNALAGKPLPVYGDGMQVRDWLYVKDHCSAIRRVLEA
GKTGEVYNVGGWNEKPNIEIVNIVCTLLDELRPRADGKNYREQISYVKDRPGHDRRYAIDASKIQRELGWKPAETFETGI
RKTVQWYLANPDWVAHVQSGAYREWTAKQYEYGQTQKAAA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=36.144578313253, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI42516563, Length=342, Percent_Identity=23.3918128654971, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI56237023, Length=360, Percent_Identity=28.0555555555556, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI56118217, Length=360, Percent_Identity=28.0555555555556, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI189083684, Length=360, Percent_Identity=28.0555555555556, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI4504031, Length=295, Percent_Identity=26.1016949152542, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788353, Length=358, Percent_Identity=61.1731843575419, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=61.3372093023256, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1786974, Length=361, Percent_Identity=27.4238227146814, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1788366, Length=357, Percent_Identity=25.7703081232493, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1788365, Length=306, Percent_Identity=25.1633986928105, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=330, Percent_Identity=35.1515151515151, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=32.9268292682927, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17539532, Length=338, Percent_Identity=25.1479289940828, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=287, Percent_Identity=26.4808362369338, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI133901786, Length=286, Percent_Identity=25.1748251748252, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17539422, Length=286, Percent_Identity=25.1748251748252, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=286, Percent_Identity=25.1748251748252, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=286, Percent_Identity=25.1748251748252, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=286, Percent_Identity=25.1748251748252, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=365, Percent_Identity=23.2876712328767, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=25.6797583081571, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=26.2686567164179, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40253; Mature: 40253

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFVTGGAGFIGANFVLDWLAQTDEPVVNIDNLTYAGNLENLSSLQADPRHVFVKGDIGD
CEEEECCCCHHHHHHHHHHHHCCCCCEEEECCEEEECCCHHHHHCCCCCCEEEEECCCCC
SALVGRLLATHQPRAILNFAAESHVDRSIHGPEDFIQTNIVGTFRLLEAVRAYWGGLQGD
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AKAEFRFLHVSTDEVYGSLAPADAAFTETHRYEPNSPYSASKAASDHLVRAYHHTYGLPV
CCCEEEEEEEEHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPYHFPEKLIPLMIVNALAGKPLPVYGDGMQVRDWLYVKDHCSAIRRVLEA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCEEEHEEEHHHHHHHHHHHHHC
GKTGEVYNVGGWNEKPNIEIVNIVCTLLDELRPRADGKNYREQISYVKDRPGHDRRYAID
CCCCCEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
ASKIQRELGWKPAETFETGIRKTVQWYLANPDWVAHVQSGAYREWTAKQYEYGQTQKAAA
HHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIFVTGGAGFIGANFVLDWLAQTDEPVVNIDNLTYAGNLENLSSLQADPRHVFVKGDIGD
CEEEECCCCHHHHHHHHHHHHCCCCCEEEECCEEEECCCHHHHHCCCCCCEEEEECCCCC
SALVGRLLATHQPRAILNFAAESHVDRSIHGPEDFIQTNIVGTFRLLEAVRAYWGGLQGD
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
AKAEFRFLHVSTDEVYGSLAPADAAFTETHRYEPNSPYSASKAASDHLVRAYHHTYGLPV
CCCEEEEEEEEHHHHHCCCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
LTTNCSNNYGPYHFPEKLIPLMIVNALAGKPLPVYGDGMQVRDWLYVKDHCSAIRRVLEA
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCCEEEHEEEHHHHHHHHHHHHHC
GKTGEVYNVGGWNEKPNIEIVNIVCTLLDELRPRADGKNYREQISYVKDRPGHDRRYAID
CCCCCEEECCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
ASKIQRELGWKPAETFETGIRKTVQWYLANPDWVAHVQSGAYREWTAKQYEYGQTQKAAA
HHHHHHHHCCCCHHHHHHHHHHHEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]