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The map label for this gene is otsB [H]

Identifier: 91789756

GI number: 91789756

Start: 4109601

End: 4110383

Strand: Direct

Name: otsB [H]

Synonym: Bpro_3915

Alternate gene names: 91789756

Gene position: 4109601-4110383 (Clockwise)

Preceding gene: 91789755

Following gene: 91789757

Centisome position: 79.03

GC content: 68.2

Gene sequence:

>783_bases
ATGCAGCTTCTCACCCCATCCCCGCAAATGGGCTCACCCCGCCTGCCCCTGATCGGGCCGCAGACCGCACTTTTTCTCGA
CTTCGACGGCACACTGGCTGACTTGGCGCCGCAGCCCGGGGCCGTGCAGGTGACCGCAGGGTTGATTCCCACCCTGGCCC
AGCTTGCGACCCGGCTCGGTGGCGCGCTGGCCATTGTGTCGGGGCGCACACTCACGGACCTGGACCGGTTTCTCGACCCC
CTGCAACTGGCCGCCGCGGCCGAGCACGGAGCGCAGCGCCGCCAGGCAGATGGCCAGGTGGTCAGCGTGGCCGCCCCCGA
TTTGCAGGAGGTGGTGCGCCGAGCCGAGGCGCTGGCTGCACGCCATCCCGGCCTGTACCTCGAAATCAAGTCCGCGGCCG
TCGCCCTGCACTACCGCCATGCCCCCGACCTCGAAACGCTGGCGTTGCAGGTCATGCGGGAAGCGGCCAACAGCACACCG
GGCGCAGAATTGCTGCAGGGCAAGTATGTCTTTGAAATCAAACCGGCCGGCGTCAGCAAGGGCACGGCCATTGAGGCTTT
CATGGGAGAAATGCCGTTTGTGGGTCGCCTGCCCCTGTTCGCCGGCGACGACGTGACCGATGAAGCCGGGTTCTCCGTGG
TGCAGTTCCTCGGCGGCCAAGGCATCAAGGTTGGCGAAGGCGCCACCCTGGCCAGGCACCGGTGCGCGGCGCCGGCCGCA
CTGCGGCAGTGGCTGCAGGCTGCCAGCCAGAATTCGGGCCACTGGCGCGGGATCAGCCCATGA

Upstream 100 bases:

>100_bases
TTTGCGGCGGGCTCCCCCGGCTGATCTTTCGACCGAAAATTGTGAAAAAGCGAAGCCGGCCTACAGCCATGTCGCACGTG
TTTTGCTAAGGTGAATGCCG

Downstream 100 bases:

>100_bases
CGGCCCCGAACGACTCCGCGCGCTTTGTGCCGCCCGCTGAACCCTCGCTCGCGATGGGCGTGATCGGCAACTGCGCGTTC
AGCGCGCTGATTGACATCCG

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Osmoregulatory trehalose synthesis protein B; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MQLLTPSPQMGSPRLPLIGPQTALFLDFDGTLADLAPQPGAVQVTAGLIPTLAQLATRLGGALAIVSGRTLTDLDRFLDP
LQLAAAAEHGAQRRQADGQVVSVAAPDLQEVVRRAEALAARHPGLYLEIKSAAVALHYRHAPDLETLALQVMREAANSTP
GAELLQGKYVFEIKPAGVSKGTAIEAFMGEMPFVGRLPLFAGDDVTDEAGFSVVQFLGGQGIKVGEGATLARHRCAAPAA
LRQWLQAASQNSGHWRGISP

Sequences:

>Translated_260_residues
MQLLTPSPQMGSPRLPLIGPQTALFLDFDGTLADLAPQPGAVQVTAGLIPTLAQLATRLGGALAIVSGRTLTDLDRFLDP
LQLAAAAEHGAQRRQADGQVVSVAAPDLQEVVRRAEALAARHPGLYLEIKSAAVALHYRHAPDLETLALQVMREAANSTP
GAELLQGKYVFEIKPAGVSKGTAIEAFMGEMPFVGRLPLFAGDDVTDEAGFSVVQFLGGQGIKVGEGATLARHRCAAPAA
LRQWLQAASQNSGHWRGISP
>Mature_260_residues
MQLLTPSPQMGSPRLPLIGPQTALFLDFDGTLADLAPQPGAVQVTAGLIPTLAQLATRLGGALAIVSGRTLTDLDRFLDP
LQLAAAAEHGAQRRQADGQVVSVAAPDLQEVVRRAEALAARHPGLYLEIKSAAVALHYRHAPDLETLALQVMREAANSTP
GAELLQGKYVFEIKPAGVSKGTAIEAFMGEMPFVGRLPLFAGDDVTDEAGFSVVQFLGGQGIKVGEGATLARHRCAAPAA
LRQWLQAASQNSGHWRGISP

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=233, Percent_Identity=43.3476394849785, Blast_Score=183, Evalue=9e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 27319; Mature: 27319

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLLTPSPQMGSPRLPLIGPQTALFLDFDGTLADLAPQPGAVQVTAGLIPTLAQLATRLG
CCCCCCCCCCCCCCCCEECCCEEEEEECCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHC
GALAIVSGRTLTDLDRFLDPLQLAAAAEHGAQRRQADGQVVSVAAPDLQEVVRRAEALAA
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
RHPGLYLEIKSAAVALHYRHAPDLETLALQVMREAANSTPGAELLQGKYVFEIKPAGVSK
CCCCEEEEEECEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCC
GTAIEAFMGEMPFVGRLPLFAGDDVTDEAGFSVVQFLGGQGIKVGEGATLARHRCAAPAA
CCHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHCCHHH
LRQWLQAASQNSGHWRGISP
HHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MQLLTPSPQMGSPRLPLIGPQTALFLDFDGTLADLAPQPGAVQVTAGLIPTLAQLATRLG
CCCCCCCCCCCCCCCCEECCCEEEEEECCCCHHHCCCCCCCEEEHHHHHHHHHHHHHHHC
GALAIVSGRTLTDLDRFLDPLQLAAAAEHGAQRRQADGQVVSVAAPDLQEVVRRAEALAA
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHH
RHPGLYLEIKSAAVALHYRHAPDLETLALQVMREAANSTPGAELLQGKYVFEIKPAGVSK
CCCCEEEEEECEEEEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCCEEEEEECCCCCC
GTAIEAFMGEMPFVGRLPLFAGDDVTDEAGFSVVQFLGGQGIKVGEGATLARHRCAAPAA
CCHHHHHHCCCCCCCCCCEECCCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHCCHHH
LRQWLQAASQNSGHWRGISP
HHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8045430; 9097040; 9278503 [H]