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The map label for this gene is lspA

Identifier: 91789694

GI number: 91789694

Start: 4042877

End: 4043371

Strand: Direct

Name: lspA

Synonym: Bpro_3850

Alternate gene names: 91789694

Gene position: 4042877-4043371 (Clockwise)

Preceding gene: 91789693

Following gene: 91789695

Centisome position: 77.74

GC content: 58.79

Gene sequence:

>495_bases
ATGGCCAAGGCTTTCGGAAAAACCTCGGGTTCCATGCTGCCCTGGCTGGGGCTGGCACTGATTCTGCTAATTGCCGACCA
GTTCACCAAGGTGCTGATCCTGGGCTATTACCGCCTGGGCGATGCCACCTATGTGACCAGTTTCTTCAATGTGGTGCGGG
TTCACAACACCGGCGCCGCGTTTTCCTTTCTGGCCTCAGCGTCGGGCTGGCAGCGCTGGTTTTTTACCGGTCTTGGTATT
GCCGCAGCGGTCTTCATTGTGTGGCTGCTCAGGTCTCATGCCGGGCAAAAGCTGTTTTCGTTCGCGCTGGCCTGCATCCT
GGGCGGTGCAGTCGGCAACGTGATCGACCGCACCCTGCACGGCTACGTGGTGGACTTTCTGGACTTCCACTACGGCAACT
GGCATTTTCCGGCCTTCAACATCGCCGACAGCGCCATCACCATTGGCGCGATTTTTCTGGTGCTGGACGAGTTGCGGCGC
GTGCGCAGAAGTTGA

Upstream 100 bases:

>100_bases
ACTACCGCGAAGACGTGGGCCATGACCCGGCGCACCCGACCATTTGCGGCCGCTGCACCAGCAACCTGTATGGCGCCGGC
GAAGACAGGAAGTACGCCTG

Downstream 100 bases:

>100_bases
CCCGGCCCCGCGCTCGCGCCCCTTGCCCGGCGTGTGGCTTCAGAGTGTGGCTTTAACTTCACAGTGCCGGGGTCAGGCCG
CTTGTGCCGCAACCCACTGG

Product: lipoprotein signal peptidase

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MAKAFGKTSGSMLPWLGLALILLIADQFTKVLILGYYRLGDATYVTSFFNVVRVHNTGAAFSFLASASGWQRWFFTGLGI
AAAVFIVWLLRSHAGQKLFSFALACILGGAVGNVIDRTLHGYVVDFLDFHYGNWHFPAFNIADSAITIGAIFLVLDELRR
VRRS

Sequences:

>Translated_164_residues
MAKAFGKTSGSMLPWLGLALILLIADQFTKVLILGYYRLGDATYVTSFFNVVRVHNTGAAFSFLASASGWQRWFFTGLGI
AAAVFIVWLLRSHAGQKLFSFALACILGGAVGNVIDRTLHGYVVDFLDFHYGNWHFPAFNIADSAITIGAIFLVLDELRR
VRRS
>Mature_163_residues
AKAFGKTSGSMLPWLGLALILLIADQFTKVLILGYYRLGDATYVTSFFNVVRVHNTGAAFSFLASASGWQRWFFTGLGIA
AAVFIVWLLRSHAGQKLFSFALACILGGAVGNVIDRTLHGYVVDFLDFHYGNWHFPAFNIADSAITIGAIFLVLDELRRV
RRS

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=145, Percent_Identity=49.6551724137931, Blast_Score=136, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_POLSJ (Q125P4)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_550646.1
- STRING:   Q125P4
- MEROPS:   A08.001
- GeneID:   4013491
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3850
- NMPDR:   fig|296591.1.peg.1486
- eggNOG:   COG0597
- HOGENOM:   HBG724422
- OMA:   ITIWIAK
- PhylomeDB:   Q125P4
- ProtClustDB:   PRK00376
- BioCyc:   PSP296591:BPRO_3850-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 18097; Mature: 17966

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 116-116 ACT_SITE 143-143

Signals:

None

Transmembrane regions:

HASH(0xaf038c4)-; HASH(0xba6db70)-; HASH(0xafc265c)-; HASH(0x16bce484)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAFGKTSGSMLPWLGLALILLIADQFTKVLILGYYRLGDATYVTSFFNVVRVHNTGAA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHH
FSFLASASGWQRWFFTGLGIAAAVFIVWLLRSHAGQKLFSFALACILGGAVGNVIDRTLH
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
GYVVDFLDFHYGNWHFPAFNIADSAITIGAIFLVLDELRRVRRS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure 
AKAFGKTSGSMLPWLGLALILLIADQFTKVLILGYYRLGDATYVTSFFNVVRVHNTGAA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHH
FSFLASASGWQRWFFTGLGIAAAVFIVWLLRSHAGQKLFSFALACILGGAVGNVIDRTLH
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
GYVVDFLDFHYGNWHFPAFNIADSAITIGAIFLVLDELRRVRRS
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA