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The map label for this gene is pcnB [H]

Identifier: 91789415

GI number: 91789415

Start: 3760090

End: 3761742

Strand: Reverse

Name: pcnB [H]

Synonym: Bpro_3561

Alternate gene names: 91789415

Gene position: 3761742-3760090 (Counterclockwise)

Preceding gene: 91789416

Following gene: 91789414

Centisome position: 72.34

GC content: 62.79

Gene sequence:

>1653_bases
ATGATCAAAAAATTCATCGACAAGCTGTTTGGCAAATCCGGCAGCACCGGGTCCGCTGCTTCCGAAAGCAAACGGATCAA
AAAATTACCCTTCGGCAAGCGCCAGGACGTCGGCCTCAAAGAACACGGCATCGACCCCAAGCTGGTCGACGATCGTGCCA
TGGATGTCGTGCGTACCTTGAAGCAGGCCGGGTTTGAAGCCTATGTGGTGGGTGGTGCCGTGCGCGACCTGCTGGTCGGC
CTGCGCCCCAAGGACTTTGATGTGGCCACCGATGCCACACCCGAGCAGGTCAAGAGCCTCTTTCGCCGCGCCTTCATCAT
CGGCCGGCGCTTTCGCATCGTTCACGTGATTTACGGCCGTGGCCGCGAGCATGAAGTCATTGAGGTCTCGATCTTCCGCG
CGCACCTGGACAGCAGTCTGGCCGAACAGGTGGGCGGCAACGAGCGCACCAGCAAGAGCGAACTCGCCGGCATGAAGCAT
GCCGTCGACAGCTCGGGCCGCGTGCTGCGCGACAACGTCTGGGGTCCGCTGGAAGAAGACGCGGCGCGGCGCGACTTCAC
CATCAACGCCATGTACTACGACCCGGAAACCCAGATCGTGGTCGACTACCACAACGGCATTCGCGATGCCAAGAAAAAGA
TCATCCGCATGATTGGCAACCCGGCCGTGCGCTACCGTGAAGACCCGGTGCGCATCATTCGCGCCGTGCGCTTCGGTGCC
AAGCTCAGCGCGCTGGGGTTCAAGTTTGAAGACAAGACCTCAGCGCCCTTGCGCGAGATGAGCAGCCTGCTCGCCGATGT
GCCGCAGTCGCGACTGTTCGACGAAATGCTCAAGCTGCTGCAAACAGGCCATTCGCTGGCCAGCGTTGAGCAACTCAAGA
CCCTGGGCCTGGGTACTGGCATCTACCCGCTGCTGGATGTGGTGGTCGAGGCCGCCCATGAGCCCTTCCTGAACCTGGCG
CTCAAGGACACCGATCGCCGCGTGGGCGAAGGCAAACCGGTGGCGCCCAGCTTTTTGCTGTCGTGCATTCTGTGGTCCGA
TGTGCGCGACGGCTGGGCACGGCGCCGCAAGCGGGGCGAGCACCTGATGCCGGCGCTGCAGGACGCGATTGATGATGCGT
TCAATGCCAAGATCGGCGATGTCTCGGGCCGCGGCAAGCTGGGTGCCGACATGCGCGAAATTTGGACCATGCAGCCGCGT
TTTGAAAAACGTGTGGGCAGCTCACCCTATTCATTGCTGGAGCAGCCGCGCTTTCGCGCCGGGTTCGACTTTTTGCGCCT
GCGCGCGCAGACCGGCGAAGTCGACATGGCGCTGGCCGAGTGGTGGGAAAAATTCAGCACCGCGTATGACGATGAGCGCC
ACGCCATGATTGAAGAGGCGCGCCAGGAGCAACTGCAAAAGCCGCAGCATCCGCGCACGCGCGTGAAGCGCCAGGACGTG
GAGGCGGACGGGTCATCCGCCAGTCCGCCCAGTGTCCGCACGGACGCCGATCAACCCGAAACCTCGTCAGAACAAGGTGA
CATGGCGCCGGCCCGAAAACGTCGCCGGCGTCGCAAACCGGCGAATCGCGCCAATGCGACCGATTCCGGCAGTGCAGAAG
GCGGCGCTGGTGGCGGCGACCAGTCCTCGCCCGGTGTTGCCTCCAACCCTTGA

Upstream 100 bases:

>100_bases
CGCCACCGAGCGCGGATGGCGCATACTTGACCTGTTCTAATTGGCAAGGGCGGCGCCAGCCGCCCCGCAACGACGGGTTC
CCCCGAAAAATTCTGTTTTC

Downstream 100 bases:

>100_bases
GCCTTGTTGAGCCGTAAGCCATGGCCGCTGAGCGCGTCGTCCAGGCCTATATCGCCTTGGGAGCCAACCTCGGTGATGCG
GCGGCCGCGCTGAGACGGGC

Product: poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 550; Mature: 550

Protein sequence:

>550_residues
MIKKFIDKLFGKSGSTGSAASESKRIKKLPFGKRQDVGLKEHGIDPKLVDDRAMDVVRTLKQAGFEAYVVGGAVRDLLVG
LRPKDFDVATDATPEQVKSLFRRAFIIGRRFRIVHVIYGRGREHEVIEVSIFRAHLDSSLAEQVGGNERTSKSELAGMKH
AVDSSGRVLRDNVWGPLEEDAARRDFTINAMYYDPETQIVVDYHNGIRDAKKKIIRMIGNPAVRYREDPVRIIRAVRFGA
KLSALGFKFEDKTSAPLREMSSLLADVPQSRLFDEMLKLLQTGHSLASVEQLKTLGLGTGIYPLLDVVVEAAHEPFLNLA
LKDTDRRVGEGKPVAPSFLLSCILWSDVRDGWARRRKRGEHLMPALQDAIDDAFNAKIGDVSGRGKLGADMREIWTMQPR
FEKRVGSSPYSLLEQPRFRAGFDFLRLRAQTGEVDMALAEWWEKFSTAYDDERHAMIEEARQEQLQKPQHPRTRVKRQDV
EADGSSASPPSVRTDADQPETSSEQGDMAPARKRRRRRKPANRANATDSGSAEGGAGGGDQSSPGVASNP

Sequences:

>Translated_550_residues
MIKKFIDKLFGKSGSTGSAASESKRIKKLPFGKRQDVGLKEHGIDPKLVDDRAMDVVRTLKQAGFEAYVVGGAVRDLLVG
LRPKDFDVATDATPEQVKSLFRRAFIIGRRFRIVHVIYGRGREHEVIEVSIFRAHLDSSLAEQVGGNERTSKSELAGMKH
AVDSSGRVLRDNVWGPLEEDAARRDFTINAMYYDPETQIVVDYHNGIRDAKKKIIRMIGNPAVRYREDPVRIIRAVRFGA
KLSALGFKFEDKTSAPLREMSSLLADVPQSRLFDEMLKLLQTGHSLASVEQLKTLGLGTGIYPLLDVVVEAAHEPFLNLA
LKDTDRRVGEGKPVAPSFLLSCILWSDVRDGWARRRKRGEHLMPALQDAIDDAFNAKIGDVSGRGKLGADMREIWTMQPR
FEKRVGSSPYSLLEQPRFRAGFDFLRLRAQTGEVDMALAEWWEKFSTAYDDERHAMIEEARQEQLQKPQHPRTRVKRQDV
EADGSSASPPSVRTDADQPETSSEQGDMAPARKRRRRRKPANRANATDSGSAEGGAGGGDQSSPGVASNP
>Mature_550_residues
MIKKFIDKLFGKSGSTGSAASESKRIKKLPFGKRQDVGLKEHGIDPKLVDDRAMDVVRTLKQAGFEAYVVGGAVRDLLVG
LRPKDFDVATDATPEQVKSLFRRAFIIGRRFRIVHVIYGRGREHEVIEVSIFRAHLDSSLAEQVGGNERTSKSELAGMKH
AVDSSGRVLRDNVWGPLEEDAARRDFTINAMYYDPETQIVVDYHNGIRDAKKKIIRMIGNPAVRYREDPVRIIRAVRFGA
KLSALGFKFEDKTSAPLREMSSLLADVPQSRLFDEMLKLLQTGHSLASVEQLKTLGLGTGIYPLLDVVVEAAHEPFLNLA
LKDTDRRVGEGKPVAPSFLLSCILWSDVRDGWARRRKRGEHLMPALQDAIDDAFNAKIGDVSGRGKLGADMREIWTMQPR
FEKRVGSSPYSLLEQPRFRAGFDFLRLRAQTGEVDMALAEWWEKFSTAYDDERHAMIEEARQEQLQKPQHPRTRVKRQDV
EADGSSASPPSVRTDADQPETSSEQGDMAPARKRRRRRKPANRANATDSGSAEGGAGGGDQSSPGVASNP

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=251, Percent_Identity=29.4820717131474, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI87081691, Length=470, Percent_Identity=35.7446808510638, Blast_Score=295, Evalue=7e-81,
Organism=Escherichia coli, GI1789436, Length=246, Percent_Identity=30.8943089430894, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=217, Percent_Identity=30.4147465437788, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281360112, Length=282, Percent_Identity=28.0141843971631, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21357337, Length=282, Percent_Identity=28.0141843971631, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 61286; Mature: 61286

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKFIDKLFGKSGSTGSAASESKRIKKLPFGKRQDVGLKEHGIDPKLVDDRAMDVVRTL
CHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHH
KQAGFEAYVVGGAVRDLLVGLRPKDFDVATDATPEQVKSLFRRAFIIGRRFRIVHVIYGR
HHCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEEEEECC
GREHEVIEVSIFRAHLDSSLAEQVGGNERTSKSELAGMKHAVDSSGRVLRDNVWGPLEED
CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHH
AARRDFTINAMYYDPETQIVVDYHNGIRDAKKKIIRMIGNPAVRYREDPVRIIRAVRFGA
HCCCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCC
KLSALGFKFEDKTSAPLREMSSLLADVPQSRLFDEMLKLLQTGHSLASVEQLKTLGLGTG
HHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
IYPLLDVVVEAAHEPFLNLALKDTDRRVGEGKPVAPSFLLSCILWSDVRDGWARRRKRGE
HHHHHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
HLMPALQDAIDDAFNAKIGDVSGRGKLGADMREIWTMQPRFEKRVGSSPYSLLEQPRFRA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHCCCCCHHHHHCCCHHH
GFDFLRLRAQTGEVDMALAEWWEKFSTAYDDERHAMIEEARQEQLQKPQHPRTRVKRQDV
CHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
EADGSSASPPSVRTDADQPETSSEQGDMAPARKRRRRRKPANRANATDSGSAEGGAGGGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QSSPGVASNP
CCCCCCCCCC
>Mature Secondary Structure
MIKKFIDKLFGKSGSTGSAASESKRIKKLPFGKRQDVGLKEHGIDPKLVDDRAMDVVRTL
CHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHH
KQAGFEAYVVGGAVRDLLVGLRPKDFDVATDATPEQVKSLFRRAFIIGRRFRIVHVIYGR
HHCCCEEEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEEEEECC
GREHEVIEVSIFRAHLDSSLAEQVGGNERTSKSELAGMKHAVDSSGRVLRDNVWGPLEED
CCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHH
AARRDFTINAMYYDPETQIVVDYHNGIRDAKKKIIRMIGNPAVRYREDPVRIIRAVRFGA
HCCCCEEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHCCCCHHCCCCHHHHHHHHHHCC
KLSALGFKFEDKTSAPLREMSSLLADVPQSRLFDEMLKLLQTGHSLASVEQLKTLGLGTG
HHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
IYPLLDVVVEAAHEPFLNLALKDTDRRVGEGKPVAPSFLLSCILWSDVRDGWARRRKRGE
HHHHHHHHHHHHHCHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCH
HLMPALQDAIDDAFNAKIGDVSGRGKLGADMREIWTMQPRFEKRVGSSPYSLLEQPRFRA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHCCCCCHHHHHCCCHHH
GFDFLRLRAQTGEVDMALAEWWEKFSTAYDDERHAMIEEARQEQLQKPQHPRTRVKRQDV
CHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
EADGSSASPPSVRTDADQPETSSEQGDMAPARKRRRRRKPANRANATDSGSAEGGAGGGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QSSPGVASNP
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]