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The map label for this gene is livK [C]

Identifier: 91789123

GI number: 91789123

Start: 3453753

End: 3455018

Strand: Reverse

Name: livK [C]

Synonym: Bpro_3263

Alternate gene names: 91789123

Gene position: 3455018-3453753 (Counterclockwise)

Preceding gene: 91789125

Following gene: 91789122

Centisome position: 66.44

GC content: 63.74

Gene sequence:

>1266_bases
ATGCAAAAACTCTTGAGACGCGTCGCGCTGGCCCTATGTCTGGGCACTGCATTGGCCGCCCAGGCGCAGCCGGCTGTCCA
GCCGCCGGTGCGGATCGCGCTCATCGAGAGCCTCTCCGGGCCCTTCGCCAACACCGGTGAAGCGGTGTTCCGCAACCTGG
TCTGGGCTACCGAGCGCGTCAATGCGCGCGGCGGCGTCAAGCTGCCCGGCGGTGCGCGCAACCTGGCGGTCGAGCGCTAC
GACAACAAGGGGCAGAACGAGGAGGCCCTGGCGGCGTTGCGTTCGGCCATCGATGACGGCGTGACGGTGATCGCGCAGGG
CAATTCGTCGGCCATAGCGGCAACGCTGATTGATGCCATCAACAAGCACAACGAGCGTGAGCCGGGCAAGCGCGTGCTGT
TCCTGAACTATTCGGCGGTCGATCCCATCCTGACGAACGAAAAGTGCAGCTACTGGCATTTCCGTTTTGATGCCCACGCC
GACATGCGCATGGCGGCCCTGATGGATGTGCTCAAGGATGACAAGGCGGTCAAAAGCGTCTACCTGATCGGCCAGGACTA
CAGCTTTGGCCAGGCGGTGCTGCGTGAGGCCCGCAAGCAGCTTGGCGCGCAGCGTCCCGATGTGCAGATCGTCGGGGACG
AGTTGCATGCGCTGGGGCGGGTGAAGGACTTCATTCCTTACGCCGTGAAAATCAAGGCCAGCGGCGCGCAGGCGGTGCTG
ACCGGGAACTGGGGCAATGACCTGACACTGCTCATCAAGGCCGCGAAGGAAGTGGGTTATGAGGGCAAGTTCTATACCTT
CTATGGCAATGCGCTGGGCGTGCCGGCGGCGCTGGGCGATGCCGGCGTGGGCAAGGTGATTGCGGTGGCGGACTGGTTTC
CCAACGAACCCGGCGATGCGTCCGAGATTTTCTACCAGGCGTTTCGCCAGCGCTTTCCCAGGCCGCAGGATGACTATGTG
CACATGCGCATGCAGCTGATGATGGAGGCGTTTGCCCAGGGTCTTGAAAGGGCTGGCTCGACGGAGTCGGGCGCCGTGGC
TGCACAGCTTGAGCGGGCCGCCGTGGCTGTGTCAGGTCACAAGGGCGCGATGCGTGCGGCCGACCACCAGTTCCAGCAGC
CGCTGTTGGTGGCGATGATGGGTAAGCAGGGCGCACCCGGTGTCAAATTTGACGTCGAGGGGTCGGGTTACGGTTTTCGT
GTTATCAAGACCATCGCGCCCGAGCGGGCCGAGCAGCCGCACAGCTGCAAGATGGTGCGGCCCTGA

Upstream 100 bases:

>100_bases
GTTTCCCCTTACACTGCGCGGCGTTCACCATGATTGCCGCATGCTCATCAATTAGGTGTACGGACGATTTATTCAAGCGA
AAACCGGGATGTATTTATTC

Downstream 100 bases:

>100_bases
TCCAGTCGCCCCCATCCCCGTTCCCCCGTCCGGTGAGCCGGCGCAACAGGTCCATCCATTGAATTAAAGGGCAAGCATGA
GACAGGCAGTAGTGAATCTT

Product: putative transporter signal peptide protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MQKLLRRVALALCLGTALAAQAQPAVQPPVRIALIESLSGPFANTGEAVFRNLVWATERVNARGGVKLPGGARNLAVERY
DNKGQNEEALAALRSAIDDGVTVIAQGNSSAIAATLIDAINKHNEREPGKRVLFLNYSAVDPILTNEKCSYWHFRFDAHA
DMRMAALMDVLKDDKAVKSVYLIGQDYSFGQAVLREARKQLGAQRPDVQIVGDELHALGRVKDFIPYAVKIKASGAQAVL
TGNWGNDLTLLIKAAKEVGYEGKFYTFYGNALGVPAALGDAGVGKVIAVADWFPNEPGDASEIFYQAFRQRFPRPQDDYV
HMRMQLMMEAFAQGLERAGSTESGAVAAQLERAAVAVSGHKGAMRAADHQFQQPLLVAMMGKQGAPGVKFDVEGSGYGFR
VIKTIAPERAEQPHSCKMVRP

Sequences:

>Translated_421_residues
MQKLLRRVALALCLGTALAAQAQPAVQPPVRIALIESLSGPFANTGEAVFRNLVWATERVNARGGVKLPGGARNLAVERY
DNKGQNEEALAALRSAIDDGVTVIAQGNSSAIAATLIDAINKHNEREPGKRVLFLNYSAVDPILTNEKCSYWHFRFDAHA
DMRMAALMDVLKDDKAVKSVYLIGQDYSFGQAVLREARKQLGAQRPDVQIVGDELHALGRVKDFIPYAVKIKASGAQAVL
TGNWGNDLTLLIKAAKEVGYEGKFYTFYGNALGVPAALGDAGVGKVIAVADWFPNEPGDASEIFYQAFRQRFPRPQDDYV
HMRMQLMMEAFAQGLERAGSTESGAVAAQLERAAVAVSGHKGAMRAADHQFQQPLLVAMMGKQGAPGVKFDVEGSGYGFR
VIKTIAPERAEQPHSCKMVRP
>Mature_421_residues
MQKLLRRVALALCLGTALAAQAQPAVQPPVRIALIESLSGPFANTGEAVFRNLVWATERVNARGGVKLPGGARNLAVERY
DNKGQNEEALAALRSAIDDGVTVIAQGNSSAIAATLIDAINKHNEREPGKRVLFLNYSAVDPILTNEKCSYWHFRFDAHA
DMRMAALMDVLKDDKAVKSVYLIGQDYSFGQAVLREARKQLGAQRPDVQIVGDELHALGRVKDFIPYAVKIKASGAQAVL
TGNWGNDLTLLIKAAKEVGYEGKFYTFYGNALGVPAALGDAGVGKVIAVADWFPNEPGDASEIFYQAFRQRFPRPQDDYV
HMRMQLMMEAFAQGLERAGSTESGAVAAQLERAAVAVSGHKGAMRAADHQFQQPLLVAMMGKQGAPGVKFDVEGSGYGFR
VIKTIAPERAEQPHSCKMVRP

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=351, Percent_Identity=22.7920227920228, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45623; Mature: 45623

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLLRRVALALCLGTALAAQAQPAVQPPVRIALIESLSGPFANTGEAVFRNLVWATERV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHC
NARGGVKLPGGARNLAVERYDNKGQNEEALAALRSAIDDGVTVIAQGNSSAIAATLIDAI
CCCCCEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
NKHNEREPGKRVLFLNYSAVDPILTNEKCSYWHFRFDAHADMRMAALMDVLKDDKAVKSV
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHEEE
YLIGQDYSFGQAVLREARKQLGAQRPDVQIVGDELHALGRVKDFIPYAVKIKASGAQAVL
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCEEEEEEECCCEEEE
TGNWGNDLTLLIKAAKEVGYEGKFYTFYGNALGVPAALGDAGVGKVIAVADWFPNEPGDA
ECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHCCCCCCCCEEEEEECCCCCCCCH
SEIFYQAFRQRFPRPQDDYVHMRMQLMMEAFAQGLERAGSTESGAVAAQLERAAVAVSGH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEECCC
KGAMRAADHQFQQPLLVAMMGKQGAPGVKFDVEGSGYGFRVIKTIAPERAEQPHSCKMVR
CCCHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCCCCEEEEEHHCCHHCCCCCCCCCCC
P
C
>Mature Secondary Structure
MQKLLRRVALALCLGTALAAQAQPAVQPPVRIALIESLSGPFANTGEAVFRNLVWATERV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHC
NARGGVKLPGGARNLAVERYDNKGQNEEALAALRSAIDDGVTVIAQGNSSAIAATLIDAI
CCCCCEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHH
NKHNEREPGKRVLFLNYSAVDPILTNEKCSYWHFRFDAHADMRMAALMDVLKDDKAVKSV
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHEEE
YLIGQDYSFGQAVLREARKQLGAQRPDVQIVGDELHALGRVKDFIPYAVKIKASGAQAVL
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHCCEEEEEEECCCEEEE
TGNWGNDLTLLIKAAKEVGYEGKFYTFYGNALGVPAALGDAGVGKVIAVADWFPNEPGDA
ECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCHHCCCCCCCCEEEEEECCCCCCCCH
SEIFYQAFRQRFPRPQDDYVHMRMQLMMEAFAQGLERAGSTESGAVAAQLERAAVAVSGH
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEECCC
KGAMRAADHQFQQPLLVAMMGKQGAPGVKFDVEGSGYGFRVIKTIAPERAEQPHSCKMVR
CCCHHHHHHHHHCCHHHHHHCCCCCCCCEEEECCCCCCEEEEEHHCCHHCCCCCCCCCCC
P
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA