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The map label for this gene is yfcG [H]

Identifier: 91788822

GI number: 91788822

Start: 3123956

End: 3124660

Strand: Direct

Name: yfcG [H]

Synonym: Bpro_2962

Alternate gene names: 91788822

Gene position: 3123956-3124660 (Clockwise)

Preceding gene: 91788821

Following gene: 91788824

Centisome position: 60.07

GC content: 60.0

Gene sequence:

>705_bases
ATGATCAACGTTTACTCATGGCCCACTCCCAATGGCCACAAAGTCCATGTCATGCTCGAGGAGTGCGGCTTTCGCCTCGG
CCGTGACTGGCAGGCCTTTCCGGTCAACATCGGCAAAGGCGACCAGTTCAAGCCCGACTTTCTCGCCATCAGCCCCAACA
ACAAGATTCCCGCACTGGTCGACCCCCATGGCCCGGACGGCAAGCCCATTTCGCTGTTTGAATCCGGCGCCATTTTGCTC
TACCTGGCCGCCAAAACCGGCAAGCTGCTGCCGAAAAGCGACCGGCTCAAATTCCAGGTTTTGCAATGGCTGATGTTCCA
GATGGGCAGCGTGGGCCCCATGATCGGCCAGACCCACCACTTTCGCCAGTACGCCCCCGAAAAGATCGACTACGCCGTCA
ACCGCTACACCAATGAAACCAGGCGGCTCTACGGCGTAATGGACAGGCAGCTGGCCACCAGCAAATTCATCGCCTGCAAC
CAGTACACCATTGCCGATGTGGCCATCTTCCCCTGGCTGCGCAACTGGGCCAACCATGGCATCGACTGGGCCGACTACCC
CCACCTGAAAAAATGGTTCGACCTCGTCAGCGCCCGCCCGGCCGTGCAGCGCGGCGTACAGGTACTGGCGGACCTGCGCC
GCCCGCTCACGGGCGACAAGGAACGCGACATCCTGTTTGGCAAAACACAGTACGCAAAGCGGTAA

Upstream 100 bases:

>100_bases
CCAGGACATCCGCACGTTTCAGAGCCGATCAAACAGGGTTTCTGCTTTTCTTTTACAGTGCAGCAGGCACCCTGATACTA
AAGAAAACACGGAAACACCA

Downstream 100 bases:

>100_bases
CTGCGGGGCCGGTGAGGGCACCGTCCTTATCGCACGGCGGCGTCCAGGGTAGCCTGAACGAGGTCCGCGCCGGCTTTTTG
GCGCACGCTGTCGACGATAC

Product: glutathione S-transferase-like protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MINVYSWPTPNGHKVHVMLEECGFRLGRDWQAFPVNIGKGDQFKPDFLAISPNNKIPALVDPHGPDGKPISLFESGAILL
YLAAKTGKLLPKSDRLKFQVLQWLMFQMGSVGPMIGQTHHFRQYAPEKIDYAVNRYTNETRRLYGVMDRQLATSKFIACN
QYTIADVAIFPWLRNWANHGIDWADYPHLKKWFDLVSARPAVQRGVQVLADLRRPLTGDKERDILFGKTQYAKR

Sequences:

>Translated_234_residues
MINVYSWPTPNGHKVHVMLEECGFRLGRDWQAFPVNIGKGDQFKPDFLAISPNNKIPALVDPHGPDGKPISLFESGAILL
YLAAKTGKLLPKSDRLKFQVLQWLMFQMGSVGPMIGQTHHFRQYAPEKIDYAVNRYTNETRRLYGVMDRQLATSKFIACN
QYTIADVAIFPWLRNWANHGIDWADYPHLKKWFDLVSARPAVQRGVQVLADLRRPLTGDKERDILFGKTQYAKR
>Mature_234_residues
MINVYSWPTPNGHKVHVMLEECGFRLGRDWQAFPVNIGKGDQFKPDFLAISPNNKIPALVDPHGPDGKPISLFESGAILL
YLAAKTGKLLPKSDRLKFQVLQWLMFQMGSVGPMIGQTHHFRQYAPEKIDYAVNRYTNETRRLYGVMDRQLATSKFIACN
QYTIADVAIFPWLRNWANHGIDWADYPHLKKWFDLVSARPAVQRGVQVLADLRRPLTGDKERDILFGKTQYAKR

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=208, Percent_Identity=49.5192307692308, Blast_Score=210, Evalue=7e-56,
Organism=Escherichia coli, GI87082195, Length=215, Percent_Identity=45.1162790697674, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI87081789, Length=176, Percent_Identity=28.9772727272727, Blast_Score=79, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324100, Length=237, Percent_Identity=35.0210970464135, Blast_Score=119, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28571670, Length=178, Percent_Identity=29.2134831460674, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17864592, Length=198, Percent_Identity=26.7676767676768, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17864598, Length=180, Percent_Identity=31.1111111111111, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922160, Length=195, Percent_Identity=30.2564102564103, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17933730, Length=236, Percent_Identity=24.1525423728814, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864594, Length=205, Percent_Identity=26.3414634146341, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI221378983, Length=172, Percent_Identity=27.906976744186, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24646259, Length=172, Percent_Identity=27.906976744186, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26841; Mature: 26841

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINVYSWPTPNGHKVHVMLEECGFRLGRDWQAFPVNIGKGDQFKPDFLAISPNNKIPALV
CCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCEEE
DPHGPDGKPISLFESGAILLYLAAKTGKLLPKSDRLKFQVLQWLMFQMGSVGPMIGQTHH
CCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHH
FRQYAPEKIDYAVNRYTNETRRLYGVMDRQLATSKFIACNQYTIADVAIFPWLRNWANHG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCC
IDWADYPHLKKWFDLVSARPAVQRGVQVLADLRRPLTGDKERDILFGKTQYAKR
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHCCC
>Mature Secondary Structure
MINVYSWPTPNGHKVHVMLEECGFRLGRDWQAFPVNIGKGDQFKPDFLAISPNNKIPALV
CCCCCCCCCCCCCEEEEEHHHCCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCEEE
DPHGPDGKPISLFESGAILLYLAAKTGKLLPKSDRLKFQVLQWLMFQMGSVGPMIGQTHH
CCCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCHH
FRQYAPEKIDYAVNRYTNETRRLYGVMDRQLATSKFIACNQYTIADVAIFPWLRNWANHG
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHCC
IDWADYPHLKKWFDLVSARPAVQRGVQVLADLRRPLTGDKERDILFGKTQYAKR
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]