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The map label for this gene is potH [H]

Identifier: 91788775

GI number: 91788775

Start: 3077824

End: 3079074

Strand: Direct

Name: potH [H]

Synonym: Bpro_2915

Alternate gene names: 91788775

Gene position: 3077824-3079074 (Clockwise)

Preceding gene: 91788774

Following gene: 91788776

Centisome position: 59.19

GC content: 62.35

Gene sequence:

>1251_bases
ATGAGCAGTATCACCAGCACCGTCCTTCAGGTTGGCGACGCCTGGGCGTTGCGCCGGGCACTGTCGCGCGCCGATGCCCG
CCGAAAATGGCGCGCCTTTGCGCTTACGATGCCCTTGCTGGTGTTTCTGCTGCTGACCTTGCTGATTCCGATTGCCGCCC
TGCTGCAGCGCGCCGTTGAAAACCCCGAGGTCGTGGCAGCCCTGCCGCGGACGGTGCAAAGCCTCAAGGGCTGGAACCCC
GAGACCACGCCAGCACCCGCAAGTTACGCGGCGCTGGTGGAAGACCTGGGCCACCTTTCAGACAGCGCGGATGCCGGTGC
GCTGGCGCGGCGGCTCAACTCCGAAGTGGCGGGAGCGCGCTCCCTGGTGATGGGGGCGTACCGTGCGTTGCCCGTCACCG
GTTCTCCGGAGGAGATTCAGGCAAAGCTTCTCCAGCTCGATCAACGCTGGGGAGAACAACCCTTCTGGCAGGCCATCGCC
AAAAACAGCTCTCGCTGGACGCCCGATTATTTGCTCGCTTCGGTCGACCTGCGACGCAGCAGCCAGGGTGCTGTGGAACG
CATGCCCGAAGACCAGCGCGCGTTTGGCACCATCTTGCTACGCACTTTTTACATCAGCTTTGTCGTCACGCTGTTTTGCC
TGGTGCTGGCTTACCCGCTGGCCTGGTGGCTGGCGTCATTGCCGGCACGCAAAGCGAACCTTCTGATGATTCTGGTGCTG
GTGCCCTTCTGGACCTCAATCCTGGTTCGTGTCGCGGCCTGGATCGTTTTGCTGCAATCCGAAGGTCTGGTGAACCGCGG
GCTGATGGGCATAGGTCTCATCGACAGCCCGCTGCCCCTGTTGTTCAACCGCGCCGGCGTGGTGATTGCCATGGTGCACA
TCCTGCTCCCCTTCATGATTTTGCCGCTGTACAGCGTGATGAAGAACGTGCCCGCGACCTACATTCGCGCCGCGGTCTCA
TTGGGGAGCGCGCCCCTGTCAGCGTTCTTTCGTGTGTACGTGCCGCAAACCTATCCCGGCATCAGCGCCGGGGCCTTGCT
GGTCTTCATTTTGTCGATTGGCTACTACGTCACCCCCGCGCTGCTGGGCGGCGCCGACGACCAGATGCTGAGCTATTACA
TCGCCCGCTACACCAACGTGGAAGTCAACTGGGGCATGGCCTGCGCCCTGGGGGCACTGCTGTTGACGGCCACCATGATT
CTTTACGGCGTTTACCGGCGCATCGGCAAATCCGAACTCAACCTCGGTTGA

Upstream 100 bases:

>100_bases
GCCATCCAGATGAACGTCGGCTTCTGGGCCGATCAGGGTGAGGCGCTTGAAAAACGCTTTGCCGCCTGGGCCACGCAATA
AGCCGCAGGAGCAACACCCC

Downstream 100 bases:

>100_bases
TATCCTCTTACAGGCGTCAATACATCATGTTCAAAATCAAAGGGCCAGTGTTCCCGCGCTATGCCACCTTGTCGGACAAG
GCCGGCTGGTGGCTGCTGCG

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MSSITSTVLQVGDAWALRRALSRADARRKWRAFALTMPLLVFLLLTLLIPIAALLQRAVENPEVVAALPRTVQSLKGWNP
ETTPAPASYAALVEDLGHLSDSADAGALARRLNSEVAGARSLVMGAYRALPVTGSPEEIQAKLLQLDQRWGEQPFWQAIA
KNSSRWTPDYLLASVDLRRSSQGAVERMPEDQRAFGTILLRTFYISFVVTLFCLVLAYPLAWWLASLPARKANLLMILVL
VPFWTSILVRVAAWIVLLQSEGLVNRGLMGIGLIDSPLPLLFNRAGVVIAMVHILLPFMILPLYSVMKNVPATYIRAAVS
LGSAPLSAFFRVYVPQTYPGISAGALLVFILSIGYYVTPALLGGADDQMLSYYIARYTNVEVNWGMACALGALLLTATMI
LYGVYRRIGKSELNLG

Sequences:

>Translated_416_residues
MSSITSTVLQVGDAWALRRALSRADARRKWRAFALTMPLLVFLLLTLLIPIAALLQRAVENPEVVAALPRTVQSLKGWNP
ETTPAPASYAALVEDLGHLSDSADAGALARRLNSEVAGARSLVMGAYRALPVTGSPEEIQAKLLQLDQRWGEQPFWQAIA
KNSSRWTPDYLLASVDLRRSSQGAVERMPEDQRAFGTILLRTFYISFVVTLFCLVLAYPLAWWLASLPARKANLLMILVL
VPFWTSILVRVAAWIVLLQSEGLVNRGLMGIGLIDSPLPLLFNRAGVVIAMVHILLPFMILPLYSVMKNVPATYIRAAVS
LGSAPLSAFFRVYVPQTYPGISAGALLVFILSIGYYVTPALLGGADDQMLSYYIARYTNVEVNWGMACALGALLLTATMI
LYGVYRRIGKSELNLG
>Mature_415_residues
SSITSTVLQVGDAWALRRALSRADARRKWRAFALTMPLLVFLLLTLLIPIAALLQRAVENPEVVAALPRTVQSLKGWNPE
TTPAPASYAALVEDLGHLSDSADAGALARRLNSEVAGARSLVMGAYRALPVTGSPEEIQAKLLQLDQRWGEQPFWQAIAK
NSSRWTPDYLLASVDLRRSSQGAVERMPEDQRAFGTILLRTFYISFVVTLFCLVLAYPLAWWLASLPARKANLLMILVLV
PFWTSILVRVAAWIVLLQSEGLVNRGLMGIGLIDSPLPLLFNRAGVVIAMVHILLPFMILPLYSVMKNVPATYIRAAVSL
GSAPLSAFFRVYVPQTYPGISAGALLVFILSIGYYVTPALLGGADDQMLSYYIARYTNVEVNWGMACALGALLLTATMIL
YGVYRRIGKSELNLG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=213, Percent_Identity=36.6197183098592, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1787369, Length=211, Percent_Identity=36.9668246445498, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1787713, Length=180, Percent_Identity=32.2222222222222, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 45578; Mature: 45447

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSITSTVLQVGDAWALRRALSRADARRKWRAFALTMPLLVFLLLTLLIPIAALLQRAVE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPEVVAALPRTVQSLKGWNPETTPAPASYAALVEDLGHLSDSADAGALARRLNSEVAGAR
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SLVMGAYRALPVTGSPEEIQAKLLQLDQRWGEQPFWQAIAKNSSRWTPDYLLASVDLRRS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHCC
SQGAVERMPEDQRAFGTILLRTFYISFVVTLFCLVLAYPLAWWLASLPARKANLLMILVL
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VPFWTSILVRVAAWIVLLQSEGLVNRGLMGIGLIDSPLPLLFNRAGVVIAMVHILLPFMI
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LPLYSVMKNVPATYIRAAVSLGSAPLSAFFRVYVPQTYPGISAGALLVFILSIGYYVTPA
HHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLGGADDQMLSYYIARYTNVEVNWGMACALGALLLTATMILYGVYRRIGKSELNLG
HHCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSITSTVLQVGDAWALRRALSRADARRKWRAFALTMPLLVFLLLTLLIPIAALLQRAVE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NPEVVAALPRTVQSLKGWNPETTPAPASYAALVEDLGHLSDSADAGALARRLNSEVAGAR
CCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SLVMGAYRALPVTGSPEEIQAKLLQLDQRWGEQPFWQAIAKNSSRWTPDYLLASVDLRRS
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHCC
SQGAVERMPEDQRAFGTILLRTFYISFVVTLFCLVLAYPLAWWLASLPARKANLLMILVL
CCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
VPFWTSILVRVAAWIVLLQSEGLVNRGLMGIGLIDSPLPLLFNRAGVVIAMVHILLPFMI
HHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LPLYSVMKNVPATYIRAAVSLGSAPLSAFFRVYVPQTYPGISAGALLVFILSIGYYVTPA
HHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LLGGADDQMLSYYIARYTNVEVNWGMACALGALLLTATMILYGVYRRIGKSELNLG
HHCCCHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]