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The map label for this gene is tcyB [H]

Identifier: 91788588

GI number: 91788588

Start: 2872901

End: 2873668

Strand: Direct

Name: tcyB [H]

Synonym: Bpro_2726

Alternate gene names: 91788588

Gene position: 2872901-2873668 (Clockwise)

Preceding gene: 91788587

Following gene: 91788589

Centisome position: 55.25

GC content: 62.63

Gene sequence:

>768_bases
ATGGAGCAAGTCCTCTGGTTCGGCTGGTTTCGCGCCGACATCATTTCGGAATACGCCGTGCTGTTCTGGCGCGGCCTGCA
GATGACCATCACCGTCACGCTGATCTGCATTGTGCAGGGCACGGCGCTCGGCCTGGCCATTGGCATGGCGCGACTGGCGC
AGGCACGCCACGCGCCCGCCCGGCAGCTCTGCCAGTACGCCTTGCGCTGGCCGTCCACCGTGTATGTCAGCTTTTTCCGC
GGCACGCCGCTGTTCGTGCAGATCCTGCTGATCCACTTTGCGGTGCTGCCGCTGTTCATCAACCCGGCCGACGGCCTGCT
GATTTCGGGCGAGACGGCCCGCAACATCAAACAGAACTACGGCGCCTTCCTGTCGGGCATTGTGGCGCTGACGCTAAACG
CGGGCGCCTACATCTCCGAGATCTTCCGGGCTGGCATCCAGTCAATTGACAAGGGCCAGGTGGAGGCGTCGCGCTCGCTG
GGCATGTCGTTTCCACGCACGATGTACCACGTGGTGCTGCCGCAGGCGTTTCGTCGCATGCTGCCGCCGCTGGGCAACAA
CGCCATTTCGCTGCTGAAAGACTCGTCGCTGATCTCGGCCATCGGCCTGGCCGAACTCGCTTACGCCGCGCGCACGGTGG
CCGGCGCCTATTCACGCTACTGGGAGCCCTACCTCACGATTTCGCTCATGTACTGGGTGCTGACACTGGGCCTTGCATAC
GTTGTCAAAAAACTGGAGGCTCGTTATGGACGAGGTGATTCGCGTTAA

Upstream 100 bases:

>100_bases
GTGAAAGCCGACGGAAGCTACGCGCAGATCTACAAGAAGTGGTTCCAGGCTGAAGCCCCCGTGCTACCGGCCCAATAAGC
ACTGAGCCCAAGCTTTCCTT

Downstream 100 bases:

>100_bases
CCGGCTGACCAAGCAGTTTGGTCATCTTCCCGTGCTGCGCGGCATCGACTGCACCATCAAGGCGTCCGAGGTGGTGTGCG
TCATCGGGCCGTCGGGCTCG

Product: amino acid ABC transporter permease

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MEQVLWFGWFRADIISEYAVLFWRGLQMTITVTLICIVQGTALGLAIGMARLAQARHAPARQLCQYALRWPSTVYVSFFR
GTPLFVQILLIHFAVLPLFINPADGLLISGETARNIKQNYGAFLSGIVALTLNAGAYISEIFRAGIQSIDKGQVEASRSL
GMSFPRTMYHVVLPQAFRRMLPPLGNNAISLLKDSSLISAIGLAELAYAARTVAGAYSRYWEPYLTISLMYWVLTLGLAY
VVKKLEARYGRGDSR

Sequences:

>Translated_255_residues
MEQVLWFGWFRADIISEYAVLFWRGLQMTITVTLICIVQGTALGLAIGMARLAQARHAPARQLCQYALRWPSTVYVSFFR
GTPLFVQILLIHFAVLPLFINPADGLLISGETARNIKQNYGAFLSGIVALTLNAGAYISEIFRAGIQSIDKGQVEASRSL
GMSFPRTMYHVVLPQAFRRMLPPLGNNAISLLKDSSLISAIGLAELAYAARTVAGAYSRYWEPYLTISLMYWVLTLGLAY
VVKKLEARYGRGDSR
>Mature_255_residues
MEQVLWFGWFRADIISEYAVLFWRGLQMTITVTLICIVQGTALGLAIGMARLAQARHAPARQLCQYALRWPSTVYVSFFR
GTPLFVQILLIHFAVLPLFINPADGLLISGETARNIKQNYGAFLSGIVALTLNAGAYISEIFRAGIQSIDKGQVEASRSL
GMSFPRTMYHVVLPQAFRRMLPPLGNNAISLLKDSSLISAIGLAELAYAARTVAGAYSRYWEPYLTISLMYWVLTLGLAY
VVKKLEARYGRGDSR

Specific function: Part of the ABC transporter complex tcyABC involved in L-cystine import. Probably responsible for the translocation of the substrate across the membrane (Probable) [H]

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787030, Length=228, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788226, Length=221, Percent_Identity=35.7466063348416, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786873, Length=224, Percent_Identity=33.0357142857143, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1786874, Length=226, Percent_Identity=29.2035398230088, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787087, Length=237, Percent_Identity=28.6919831223629, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI87082239, Length=235, Percent_Identity=29.3617021276596, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788645, Length=248, Percent_Identity=25.4032258064516, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI48994930, Length=115, Percent_Identity=40.8695652173913, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788646, Length=184, Percent_Identity=29.3478260869565, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI1787086, Length=198, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010065
- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28349; Mature: 28349

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVLWFGWFRADIISEYAVLFWRGLQMTITVTLICIVQGTALGLAIGMARLAQARHAPA
CCCEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RQLCQYALRWPSTVYVSFFRGTPLFVQILLIHFAVLPLFINPADGLLISGETARNIKQNY
HHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHH
GAFLSGIVALTLNAGAYISEIFRAGIQSIDKGQVEASRSLGMSFPRTMYHVVLPQAFRRM
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LPPLGNNAISLLKDSSLISAIGLAELAYAARTVAGAYSRYWEPYLTISLMYWVLTLGLAY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VVKKLEARYGRGDSR
HHHHHHHHCCCCCCC
>Mature Secondary Structure
MEQVLWFGWFRADIISEYAVLFWRGLQMTITVTLICIVQGTALGLAIGMARLAQARHAPA
CCCEEEHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RQLCQYALRWPSTVYVSFFRGTPLFVQILLIHFAVLPLFINPADGLLISGETARNIKQNY
HHHHHHHHHCCCCEEEHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHH
GAFLSGIVALTLNAGAYISEIFRAGIQSIDKGQVEASRSLGMSFPRTMYHVVLPQAFRRM
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LPPLGNNAISLLKDSSLISAIGLAELAYAARTVAGAYSRYWEPYLTISLMYWVLTLGLAY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VVKKLEARYGRGDSR
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7551042; 8969502; 9384377 [H]