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The map label for this gene is hisJ [H]

Identifier: 91788587

GI number: 91788587

Start: 2872111

End: 2872878

Strand: Direct

Name: hisJ [H]

Synonym: Bpro_2725

Alternate gene names: 91788587

Gene position: 2872111-2872878 (Clockwise)

Preceding gene: 91788586

Following gene: 91788588

Centisome position: 55.23

GC content: 62.63

Gene sequence:

>768_bases
ATGAATCTCTTGCAAAAAATAGCGGCTACGGCCGGACTGTTCACACTCGCGCTGGGCGGCGTCCATGCCCAGAACACCGA
GCTGGTGGTCGGCTCCAGCGCCACCTACCGGCCGTTTGCCTATGAAAGCCCGAGCAAGGACATCGTCGGCTACGACGTGG
ACATGATCAAGGCCATCGCCCAGAAGGCTGGCCTGAAGATCAAGATCGTCAACACGCCCTGGACCGGCATCTTCGCCGCC
CTCAACAACGGCGACCTGGACCTGGTGATCTCCGGCGTGACCATCAACGACAAGCGCAAGCAGTCCTACGACTTCAGCAC
CCCCTACTTCGAGGCGCGCCAGCTGCTCGCCGTGCACAGCAACAGCACCGCCAAAGGCCTGAAGGACCTGGCCGGCAAAA
AGATCGGTGTGGTCACCGGCAGCACTGGCGACGACATGGCCTCGCGCGAGTTCGGCAAGACCAACCCCGACATCCGCCGC
TTCGAGAGCACGCCCGTGGTGATCTCCGAGCTGGCCAACAACGGCCTGGATGCGGCCATCGGCGACAACGGCGTCATTGC
CTTCCGCGCCCAGGAGCACAAGCAGCTCAAGACCGTCAGCGACCCGGCCTTTCCCAAGGAGTACTTCGGCATCGTCGTCA
AGCAGGGCAACAAGGCCTTGCTGGACAAGCTCAACGCCGGCCTGGCCGCGGTGAAAGCCGACGGAAGCTACGCGCAGATC
TACAAGAAGTGGTTCCAGGCTGAAGCCCCCGTGCTACCGGCCCAATAA

Upstream 100 bases:

>100_bases
GTGCCCGGCTTGGCACGCGTTACCATTGGCATCTTTTCGATTTGCGGCCCTCAGCGGGCCGCTTGATGCGTTTTCCACCC
CCACCTGTTTGGACTTACCC

Downstream 100 bases:

>100_bases
GCACTGAGCCCAAGCTTTCCTTATGGAGCAAGTCCTCTGGTTCGGCTGGTTTCGCGCCGACATCATTTCGGAATACGCCG
TGCTGTTCTGGCGCGGCCTG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-glutamine [Cytoplasm] [C]

Alternate protein names: HBP [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNLLQKIAATAGLFTLALGGVHAQNTELVVGSSATYRPFAYESPSKDIVGYDVDMIKAIAQKAGLKIKIVNTPWTGIFAA
LNNGDLDLVISGVTINDKRKQSYDFSTPYFEARQLLAVHSNSTAKGLKDLAGKKIGVVTGSTGDDMASREFGKTNPDIRR
FESTPVVISELANNGLDAAIGDNGVIAFRAQEHKQLKTVSDPAFPKEYFGIVVKQGNKALLDKLNAGLAAVKADGSYAQI
YKKWFQAEAPVLPAQ

Sequences:

>Translated_255_residues
MNLLQKIAATAGLFTLALGGVHAQNTELVVGSSATYRPFAYESPSKDIVGYDVDMIKAIAQKAGLKIKIVNTPWTGIFAA
LNNGDLDLVISGVTINDKRKQSYDFSTPYFEARQLLAVHSNSTAKGLKDLAGKKIGVVTGSTGDDMASREFGKTNPDIRR
FESTPVVISELANNGLDAAIGDNGVIAFRAQEHKQLKTVSDPAFPKEYFGIVVKQGNKALLDKLNAGLAAVKADGSYAQI
YKKWFQAEAPVLPAQ
>Mature_255_residues
MNLLQKIAATAGLFTLALGGVHAQNTELVVGSSATYRPFAYESPSKDIVGYDVDMIKAIAQKAGLKIKIVNTPWTGIFAA
LNNGDLDLVISGVTINDKRKQSYDFSTPYFEARQLLAVHSNSTAKGLKDLAGKKIGVVTGSTGDDMASREFGKTNPDIRR
FESTPVVISELANNGLDAAIGDNGVIAFRAQEHKQLKTVSDPAFPKEYFGIVVKQGNKALLDKLNAGLAAVKADGSYAQI
YKKWFQAEAPVLPAQ

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787031, Length=233, Percent_Identity=33.9055793991416, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787085, Length=248, Percent_Identity=31.4516129032258, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788228, Length=223, Percent_Identity=30.4932735426009, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI1787088, Length=249, Percent_Identity=28.9156626506024, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1788649, Length=224, Percent_Identity=26.7857142857143, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788648, Length=224, Percent_Identity=24.5535714285714, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: 1440 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 460 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27335; Mature: 27335

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLQKIAATAGLFTLALGGVHAQNTELVVGSSATYRPFAYESPSKDIVGYDVDMIKAIA
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QKAGLKIKIVNTPWTGIFAALNNGDLDLVISGVTINDKRKQSYDFSTPYFEARQLLAVHS
HHCCCEEEEEECCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHEEEECC
NSTAKGLKDLAGKKIGVVTGSTGDDMASREFGKTNPDIRRFESTPVVISELANNGLDAAI
CCHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCCCHHHHCCCCHHHHHHHCCCCCEEE
GDNGVIAFRAQEHKQLKTVSDPAFPKEYFGIVVKQGNKALLDKLNAGLAAVKADGSYAQI
CCCCEEEEEECCHHHHHHCCCCCCCHHHEEEEEECCCHHHHHHHCCCEEEEECCCCHHHH
YKKWFQAEAPVLPAQ
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MNLLQKIAATAGLFTLALGGVHAQNTELVVGSSATYRPFAYESPSKDIVGYDVDMIKAIA
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
QKAGLKIKIVNTPWTGIFAALNNGDLDLVISGVTINDKRKQSYDFSTPYFEARQLLAVHS
HHCCCEEEEEECCCCEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCCHHHHHHEEEECC
NSTAKGLKDLAGKKIGVVTGSTGDDMASREFGKTNPDIRRFESTPVVISELANNGLDAAI
CCHHHHHHHHCCCEEEEEECCCCCCHHHHHCCCCCCCHHHHCCCCHHHHHHHCCCCCEEE
GDNGVIAFRAQEHKQLKTVSDPAFPKEYFGIVVKQGNKALLDKLNAGLAAVKADGSYAQI
CCCCEEEEEECCHHHHHHCCCCCCCHHHEEEEEECCCHHHHHHHCCCEEEEECCCCHHHH
YKKWFQAEAPVLPAQ
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamine [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamine [Periplasm] + H2O = ADP + phosphate + L-glutamine [Cytoplasm] [C]

General reaction: Translocates substrate across the membrane [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]