Click here to switch to the map view.

The map label for this gene is ispDF [H]

Identifier: 91788578

GI number: 91788578

Start: 2861383

End: 2862105

Strand: Reverse

Name: ispDF [H]

Synonym: Bpro_2716

Alternate gene names: 91788578

Gene position: 2862105-2861383 (Counterclockwise)

Preceding gene: 91788584

Following gene: 91788577

Centisome position: 55.04

GC content: 62.93

Gene sequence:

>723_bases
ATGACGGCAACCAGCGCAAATTCCCTCAATTCGTCCATTTCCCGTTTTTTTGCCCTGATTCCCTGTGCGGGGCAGGGCAG
CCGCGCCGGAACGCCCGGCGCCAAGCAATACCAGGTGATTGCCGGCAGGGCCATGGTGTTGCATACGCTGGCGGCTTTTT
CAGATGTTGAACGTCTTTCAGGCATGCTGGTGGTGGTGGCGCCCGGTGACCCGTTTTTTGATCAGGCCTTGAACAAGCGT
TTCGATGTGGCCGCTTGCGGCGGCGCCAGCCGCGCCGCGAGTGTGCGCAACGGCCTGCAGGCACTGGTCAACAGTGGCGC
CGAGGAGCACGACTGGGTGTTGGTCCACGATGCGGCCCGCTGCCTGATTACCCCTGCGCAAATCAACCAGCTGATCGATG
CCTGCGAACACGACGAGGTGGGTGGTTTGCTTGCGCACAAGCTGCCCGACACCCTCAAGGTGGAAGTGGGCGGGCGTGTG
GCGGCCACCATTGACCGGAACGATAAGTGGCTGGCCCAGACGCCGCAGATGTTTCGCATTGGTGCGCTGAGCCGGGCACT
CGACCTGGCCGGCCCCGCCGTGACCGACGAAGCCAGTGCCATGGAGGCCTTGGGCCTGGCGCCGAAATTGGTGGCTGGCA
GTGCGCAAAATTTCAAGGTGACCTACCCCGAAGACTTTGCACTGGCTGAGGCGATTCTGCTCACGCGCAGCCGCAGACCC
TGA

Upstream 100 bases:

>100_bases
GTTGCAGGAAAATTCAGGAAAAAAGCCGAGCATCCCAAACCATTCGGAGTGGGGTGCTCGCAGCCTGCAGAATGAGGATT
TTAGAATCTATGATGAGGCG

Downstream 100 bases:

>100_bases
AGCCGTTGCCGGGCCATCGTTGACCGCCCGGGAAACGATTCCCTTTTACTTTTATTTTCATGTGAAAGCGCATGCCATGA
ATTCCGTGCCGTCCACCCCA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MTATSANSLNSSISRFFALIPCAGQGSRAGTPGAKQYQVIAGRAMVLHTLAAFSDVERLSGMLVVVAPGDPFFDQALNKR
FDVAACGGASRAASVRNGLQALVNSGAEEHDWVLVHDAARCLITPAQINQLIDACEHDEVGGLLAHKLPDTLKVEVGGRV
AATIDRNDKWLAQTPQMFRIGALSRALDLAGPAVTDEASAMEALGLAPKLVAGSAQNFKVTYPEDFALAEAILLTRSRRP

Sequences:

>Translated_240_residues
MTATSANSLNSSISRFFALIPCAGQGSRAGTPGAKQYQVIAGRAMVLHTLAAFSDVERLSGMLVVVAPGDPFFDQALNKR
FDVAACGGASRAASVRNGLQALVNSGAEEHDWVLVHDAARCLITPAQINQLIDACEHDEVGGLLAHKLPDTLKVEVGGRV
AATIDRNDKWLAQTPQMFRIGALSRALDLAGPAVTDEASAMEALGLAPKLVAGSAQNFKVTYPEDFALAEAILLTRSRRP
>Mature_239_residues
TATSANSLNSSISRFFALIPCAGQGSRAGTPGAKQYQVIAGRAMVLHTLAAFSDVERLSGMLVVVAPGDPFFDQALNKRF
DVAACGGASRAASVRNGLQALVNSGAEEHDWVLVHDAARCLITPAQINQLIDACEHDEVGGLLAHKLPDTLKVEVGGRVA
ATIDRNDKWLAQTPQMFRIGALSRALDLAGPAVTDEASAMEALGLAPKLVAGSAQNFKVTYPEDFALAEAILLTRSRRP

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=235, Percent_Identity=25.9574468085106, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789104, Length=228, Percent_Identity=40.3508771929825, Blast_Score=131, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 25291; Mature: 25160

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATSANSLNSSISRFFALIPCAGQGSRAGTPGAKQYQVIAGRAMVLHTLAAFSDVERLS
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHC
GMLVVVAPGDPFFDQALNKRFDVAACGGASRAASVRNGLQALVNSGAEEHDWVLVHDAAR
CEEEEEECCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
CLITPAQINQLIDACEHDEVGGLLAHKLPDTLKVEVGGRVAATIDRNDKWLAQTPQMFRI
HEECHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCEEEEEEECCCCCHHCCCHHHHH
GALSRALDLAGPAVTDEASAMEALGLAPKLVAGSAQNFKVTYPEDFALAEAILLTRSRRP
HHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TATSANSLNSSISRFFALIPCAGQGSRAGTPGAKQYQVIAGRAMVLHTLAAFSDVERLS
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHC
GMLVVVAPGDPFFDQALNKRFDVAACGGASRAASVRNGLQALVNSGAEEHDWVLVHDAAR
CEEEEEECCCCHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCH
CLITPAQINQLIDACEHDEVGGLLAHKLPDTLKVEVGGRVAATIDRNDKWLAQTPQMFRI
HEECHHHHHHHHHHHCCCCCCCHHHHHCCCCEEEEECCEEEEEEECCCCCHHCCCHHHHH
GALSRALDLAGPAVTDEASAMEALGLAPKLVAGSAQNFKVTYPEDFALAEAILLTRSRRP
HHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA