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The map label for this gene is lpdA [H]

Identifier: 91788531

GI number: 91788531

Start: 2804422

End: 2806266

Strand: Reverse

Name: lpdA [H]

Synonym: Bpro_2669

Alternate gene names: 91788531

Gene position: 2806266-2804422 (Counterclockwise)

Preceding gene: 91788532

Following gene: 91788530

Centisome position: 53.96

GC content: 62.33

Gene sequence:

>1845_bases
ATGAGCGCATCTTCGAGTCTCATCGATATCAAGGTACCGGACATTGGCGACTTTGACGAAGTGTCCGTGATTGAAGTGCT
GGTCAAGGCCGGCGACACCATCAAGGCCGAGCAGAGCCTGATCACCGTGGAAAGCGACAAGGCATCGATGGAAATCCCGT
CGAGCCAGGCCGGCGTGGTTAAGGAAGTCAAGGTCAAGATTGGCGACAAGGTCAAGCAAGGTTCTATCGTTCTGCTGCTG
GAGGGCGCAGGCGCAGCACTTGCTCCAAAATCCGAAGAAAAACAGACCCCAGCCCCCGTTCCTTCCGGGCAGGCTGCTCC
TGCGAGTGCAGCAGCTCAAGCGCCGGCCCCGGCCGCCGCCAGTTTCGCCGGCACGGCAGACCTCGACTGCGACGTGCTGG
TGCTGGGCGCGGGCCCTGGCGGCTATTCGGCGGCGTTTCGCGCTGCCGATCTGGGCCTCAAGGTCGTGCTGGTAGAGCGC
TACGCGACCCTTGGCGGAGTTTGCCTCAACGTGGGCTGCATTCCATCCAAGGCGCTGCTGCACGTGGCGGCCGTCATGGA
TGAAGTCCGGCATTTCGACAGCCTGGGCGTGAGCTTTGGCGAACCGGTGCTGGACATCAACAAACTGCGCACGCACAAGG
AAAAAGTCGTCGGCAAGCTGACGGGCGGGCTCGCCGCCATGGCCAAGATGCGCAAGGTCACCGTGGTACGCGGTTACGGC
AGTTTCGTCGGTGCGCACCATGTCGAGGTGCAGGAAACCTCGGGTGCCGCGCAAGAGACCACCGGCAAGAAGCAGACCAT
TGCCTTCAAGCATTGCGTGATCGCCGCGGGCTCGCAAGCGGTGCGCCTGCCTTTCATGCCCGAGGACCCGCGCGTGGTGG
ATTCGACGGGTGCACTGAACATGAAAGAAGTGCCCAAGCGCATGCTGATTCTGGGCGGCGGCATCATCGGCCTGGAAATG
GGAACGGTCTATTCCTCGCTGGGCGCGCGCCTGGACGTGGTTGAAATGCTGGACGGGCTGATGCAGGGCGCCGACCGTGA
CCTGGTCAAGGTTTGGCAGAAGATGAATGCACCGCGCTTTGACAACATCATGCTCAAGACCAAAACGGTGGCGGCCAAGG
CGACCGCGGCTGGCATTGAAGTCAGCTTTGAAGGCGAGGGCGCTCCCGCACCGCAAACCTACGACCTGGTGCTGCAGGCC
GTGGGCCGCACGCCCAACGGTAAAAAGATCGCGGCTGAAAAAGCTGGCATCACCGTGACCGACCGCGGTTTCATCCCTGT
CGACATCCAGATGCGGACCAACGTGCCGCATATCTTCGCGATTGGCGATGTCGTGGGCCAGCCCATGCTGGCGCACAAGG
CGGTGCATGAAGCGCATGTGGCGGCTGAAGTGATTGCGGGCGAGCTGCAGGGCAACAAGGAACTGGCGAGCGCCGCTTTC
AATGCCCGCGTGATCCCGAGCGTGGCTTACACCGACCCTGAAATTGCTTGGGTAGGCCTGACCGAAGACCAGGCCAAGGC
GCAGGGCATCAAGGTCAAGAAAGGCCTGTTCCCCTGGACGGCCTCGGGCCGCGCGATTGCCAACGGCCGCGATGAAGGTT
TCACCAAGCTGCTGTTTGACGACAGCCCCGAAGCGCATGGCCATGGCAAGATCCTCGGCGGTGGCATTGTGGGCACGCAC
GCCGGTGACCTGATTGGCGAGATTGCCCTGGCGATTGAAATGGGCGCGGATGCCGTCGATATTGGCAAAACCATCCATCC
GCACCCTACGCTCGGCGAGAGCATGGGCCTGGCGGCCGAGGTGGCGCATGGCAGCTGTACCGATATTCCGCCGGCGCGCA
AGTAA

Upstream 100 bases:

>100_bases
CCCGCGAAGTAGCCCTGGCGGGCATGAATGCCGGCTGCGAGTTCGACAAGAACTCCGCTGGACCCATTGAAGTATTTACT
CTCAAATTGAAAGCCTCCAA

Downstream 100 bases:

>100_bases
GCAACCCTGCTAACCCCCTGCCAGCCCGGACCAGCGAAGGTCCGGGCTTTTTGTTGCCGGTTTCCCGGAGCAGCGCATGC
GGTCGACTTGAATTTAGTGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MSASSSLIDIKVPDIGDFDEVSVIEVLVKAGDTIKAEQSLITVESDKASMEIPSSQAGVVKEVKVKIGDKVKQGSIVLLL
EGAGAALAPKSEEKQTPAPVPSGQAAPASAAAQAPAPAAASFAGTADLDCDVLVLGAGPGGYSAAFRAADLGLKVVLVER
YATLGGVCLNVGCIPSKALLHVAAVMDEVRHFDSLGVSFGEPVLDINKLRTHKEKVVGKLTGGLAAMAKMRKVTVVRGYG
SFVGAHHVEVQETSGAAQETTGKKQTIAFKHCVIAAGSQAVRLPFMPEDPRVVDSTGALNMKEVPKRMLILGGGIIGLEM
GTVYSSLGARLDVVEMLDGLMQGADRDLVKVWQKMNAPRFDNIMLKTKTVAAKATAAGIEVSFEGEGAPAPQTYDLVLQA
VGRTPNGKKIAAEKAGITVTDRGFIPVDIQMRTNVPHIFAIGDVVGQPMLAHKAVHEAHVAAEVIAGELQGNKELASAAF
NARVIPSVAYTDPEIAWVGLTEDQAKAQGIKVKKGLFPWTASGRAIANGRDEGFTKLLFDDSPEAHGHGKILGGGIVGTH
AGDLIGEIALAIEMGADAVDIGKTIHPHPTLGESMGLAAEVAHGSCTDIPPARK

Sequences:

>Translated_614_residues
MSASSSLIDIKVPDIGDFDEVSVIEVLVKAGDTIKAEQSLITVESDKASMEIPSSQAGVVKEVKVKIGDKVKQGSIVLLL
EGAGAALAPKSEEKQTPAPVPSGQAAPASAAAQAPAPAAASFAGTADLDCDVLVLGAGPGGYSAAFRAADLGLKVVLVER
YATLGGVCLNVGCIPSKALLHVAAVMDEVRHFDSLGVSFGEPVLDINKLRTHKEKVVGKLTGGLAAMAKMRKVTVVRGYG
SFVGAHHVEVQETSGAAQETTGKKQTIAFKHCVIAAGSQAVRLPFMPEDPRVVDSTGALNMKEVPKRMLILGGGIIGLEM
GTVYSSLGARLDVVEMLDGLMQGADRDLVKVWQKMNAPRFDNIMLKTKTVAAKATAAGIEVSFEGEGAPAPQTYDLVLQA
VGRTPNGKKIAAEKAGITVTDRGFIPVDIQMRTNVPHIFAIGDVVGQPMLAHKAVHEAHVAAEVIAGELQGNKELASAAF
NARVIPSVAYTDPEIAWVGLTEDQAKAQGIKVKKGLFPWTASGRAIANGRDEGFTKLLFDDSPEAHGHGKILGGGIVGTH
AGDLIGEIALAIEMGADAVDIGKTIHPHPTLGESMGLAAEVAHGSCTDIPPARK
>Mature_613_residues
SASSSLIDIKVPDIGDFDEVSVIEVLVKAGDTIKAEQSLITVESDKASMEIPSSQAGVVKEVKVKIGDKVKQGSIVLLLE
GAGAALAPKSEEKQTPAPVPSGQAAPASAAAQAPAPAAASFAGTADLDCDVLVLGAGPGGYSAAFRAADLGLKVVLVERY
ATLGGVCLNVGCIPSKALLHVAAVMDEVRHFDSLGVSFGEPVLDINKLRTHKEKVVGKLTGGLAAMAKMRKVTVVRGYGS
FVGAHHVEVQETSGAAQETTGKKQTIAFKHCVIAAGSQAVRLPFMPEDPRVVDSTGALNMKEVPKRMLILGGGIIGLEMG
TVYSSLGARLDVVEMLDGLMQGADRDLVKVWQKMNAPRFDNIMLKTKTVAAKATAAGIEVSFEGEGAPAPQTYDLVLQAV
GRTPNGKKIAAEKAGITVTDRGFIPVDIQMRTNVPHIFAIGDVVGQPMLAHKAVHEAHVAAEVIAGELQGNKELASAAFN
ARVIPSVAYTDPEIAWVGLTEDQAKAQGIKVKKGLFPWTASGRAIANGRDEGFTKLLFDDSPEAHGHGKILGGGIVGTHA
GDLIGEIALAIEMGADAVDIGKTIHPHPTLGESMGLAAEVAHGSCTDIPPARK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=475, Percent_Identity=40.2105263157895, Blast_Score=321, Evalue=2e-87,
Organism=Homo sapiens, GI50301238, Length=479, Percent_Identity=28.1837160751566, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI291045266, Length=500, Percent_Identity=26.4, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI22035672, Length=479, Percent_Identity=28.39248434238, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI148277065, Length=453, Percent_Identity=25.1655629139073, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI148277071, Length=478, Percent_Identity=25.1046025104602, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI33519430, Length=453, Percent_Identity=25.1655629139073, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI33519428, Length=453, Percent_Identity=25.1655629139073, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI33519426, Length=453, Percent_Identity=25.1655629139073, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI291045268, Length=493, Percent_Identity=25.5578093306288, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1786307, Length=488, Percent_Identity=60.0409836065574, Blast_Score=566, Evalue=1e-162,
Organism=Escherichia coli, GI87082354, Length=494, Percent_Identity=27.7327935222672, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789915, Length=474, Percent_Identity=29.535864978903, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=26.2820512820513, Blast_Score=143, Evalue=4e-35,
Organism=Escherichia coli, GI1786305, Length=73, Percent_Identity=63.013698630137, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI32565766, Length=472, Percent_Identity=39.6186440677966, Blast_Score=318, Evalue=6e-87,
Organism=Caenorhabditis elegans, GI17557007, Length=494, Percent_Identity=26.5182186234818, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI71983429, Length=468, Percent_Identity=25, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71983419, Length=468, Percent_Identity=25, Blast_Score=99, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI71982272, Length=461, Percent_Identity=24.5119305856833, Blast_Score=98, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321091, Length=486, Percent_Identity=38.0658436213992, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6325240, Length=495, Percent_Identity=29.2929292929293, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6325166, Length=497, Percent_Identity=25.9557344064386, Blast_Score=145, Evalue=2e-35,
Organism=Drosophila melanogaster, GI21358499, Length=500, Percent_Identity=39.8, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24640551, Length=532, Percent_Identity=27.6315789473684, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640549, Length=503, Percent_Identity=27.6341948310139, Blast_Score=119, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24640553, Length=503, Percent_Identity=27.4353876739563, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17737741, Length=494, Percent_Identity=26.3157894736842, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 63685; Mature: 63554

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASSSLIDIKVPDIGDFDEVSVIEVLVKAGDTIKAEQSLITVESDKASMEIPSSQAGVV
CCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCEEEEEECCCCCEECCCCCCCCC
KEVKVKIGDKVKQGSIVLLLEGAGAALAPKSEEKQTPAPVPSGQAAPASAAAQAPAPAAA
EEEHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHH
SFAGTADLDCDVLVLGAGPGGYSAAFRAADLGLKVVLVERYATLGGVCLNVGCIPSKALL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEEHHHHHCCEEEEECCCCHHHHH
HVAAVMDEVRHFDSLGVSFGEPVLDINKLRTHKEKVVGKLTGGLAAMAKMRKVTVVRGYG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
SFVGAHHVEVQETSGAAQETTGKKQTIAFKHCVIAAGSQAVRLPFMPEDPRVVDSTGALN
CCCCCEEEEEECCCCCCHHCCCCCCEEEEEEEEEECCCCEEECCCCCCCCCEECCCCCCC
MKEVPKRMLILGGGIIGLEMGTVYSSLGARLDVVEMLDGLMQGADRDLVKVWQKMNAPRF
HHHHCCEEEEECCCEEEEEHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHCCCCCC
DNIMLKTKTVAAKATAAGIEVSFEGEGAPAPQTYDLVLQAVGRTPNGKKIAAEKAGITVT
CCEEEEEEEHHHEEEECCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEE
DRGFIPVDIQMRTNVPHIFAIGDVVGQPMLAHKAVHEAHVAAEVIAGELQGNKELASAAF
CCCEEEEEEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
NARVIPSVAYTDPEIAWVGLTEDQAKAQGIKVKKGLFPWTASGRAIANGRDEGFTKLLFD
CCEECCEEEECCCCEEEEECCCHHHHHCCEEEECCCCCCCCCCCEEECCCCCCCEEEEEC
DSPEAHGHGKILGGGIVGTHAGDLIGEIALAIEMGADAVDIGKTIHPHPTLGESMGLAAE
CCCCCCCCCEEEECCEECCCHHHHHHHHHEEEECCCCHHHCCCCCCCCCCCCCCCCCEEE
VAHGSCTDIPPARK
ECCCCCCCCCCCCC
>Mature Secondary Structure 
SASSSLIDIKVPDIGDFDEVSVIEVLVKAGDTIKAEQSLITVESDKASMEIPSSQAGVV
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCEEEEEECCCCCEECCCCCCCCC
KEVKVKIGDKVKQGSIVLLLEGAGAALAPKSEEKQTPAPVPSGQAAPASAAAQAPAPAAA
EEEHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHH
SFAGTADLDCDVLVLGAGPGGYSAAFRAADLGLKVVLVERYATLGGVCLNVGCIPSKALL
CCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEEEHHHHHCCEEEEECCCCHHHHH
HVAAVMDEVRHFDSLGVSFGEPVLDINKLRTHKEKVVGKLTGGLAAMAKMRKVTVVRGYG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECC
SFVGAHHVEVQETSGAAQETTGKKQTIAFKHCVIAAGSQAVRLPFMPEDPRVVDSTGALN
CCCCCEEEEEECCCCCCHHCCCCCCEEEEEEEEEECCCCEEECCCCCCCCCEECCCCCCC
MKEVPKRMLILGGGIIGLEMGTVYSSLGARLDVVEMLDGLMQGADRDLVKVWQKMNAPRF
HHHHCCEEEEECCCEEEEEHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHCCCCCC
DNIMLKTKTVAAKATAAGIEVSFEGEGAPAPQTYDLVLQAVGRTPNGKKIAAEKAGITVT
CCEEEEEEEHHHEEEECCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCEEEE
DRGFIPVDIQMRTNVPHIFAIGDVVGQPMLAHKAVHEAHVAAEVIAGELQGNKELASAAF
CCCEEEEEEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHC
NARVIPSVAYTDPEIAWVGLTEDQAKAQGIKVKKGLFPWTASGRAIANGRDEGFTKLLFD
CCEECCEEEECCCCEEEEECCCHHHHHCCEEEECCCCCCCCCCCEEECCCCCCCEEEEEC
DSPEAHGHGKILGGGIVGTHAGDLIGEIALAIEMGADAVDIGKTIHPHPTLGESMGLAAE
CCCCCCCCCEEEECCEECCCHHHHHHHHHEEEECCCCHHHCCCCCCCCCCCCCCCCCEEE
VAHGSCTDIPPARK
ECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]