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The map label for this gene is alkK [H]

Identifier: 91788441

GI number: 91788441

Start: 2700653

End: 2702281

Strand: Reverse

Name: alkK [H]

Synonym: Bpro_2579

Alternate gene names: 91788441

Gene position: 2702281-2700653 (Counterclockwise)

Preceding gene: 91788448

Following gene: 91788440

Centisome position: 51.96

GC content: 60.83

Gene sequence:

>1629_bases
ATGTTAGGTTTGATGCAAAGCCAGCCACTGCAGATCTCGTCGCTGATTGAGTTTGCGGAGCGTCACCATGGCGACGCTGA
AATCGTGTCGCGCCGGGTGGAAGGGGACTTGCATCGCTACACCTACAAGGACGCGGCCTGTCGCGCGCGGCAGGTGGCCC
AGGCGCTGGATGGCCTGAAGCTCGCGTTCAGCGACCGGGTTGCCACGCTGGCATGGAATGGTTACCGTCACTTTGAGTTG
TATTACGGCGTCAGCGGCTCGGGCCGCGTGCTGCACACCATCAACCCGCGCCTGCATCCTGACCAGATCGCCTGGATTGC
CAACCACGCCGAAGACCAGGTGCTGTGTTTTGACCTGACCTTTTTGCCCCTGGTGCAGGCCGTCCACGGCAAGTGCCCCA
CCATCAAACACTACATTGCGCTGTGCGATGCGGACAAGCTCCCTGCAGACACCGGCATTCCGAATCTGCAGAGCTACGAG
CGCTGGATTGGCGGGCAGACAGGTGATTACGCCTGGCCTGCTTTTGACGAGAACTCCGCTTCCAGCATGTGCTACACCAG
CGGCACCACGGGCAATCCGAAGGCAGCGCTATTCAGCCACCGTTCCAGCATCCTGCATGCGTACGCGGCCGCCTTGCCCG
ATGTCATGTGCATTTCGGCGCGTGATGCCATCTTGCCGGTCGTACCGATGTTTCACGTCAACGCGTGGGGCATTCCGTAC
TCGGCGGCGCTGGCGGGTGCCAAGCTGGTGTTTCCGGGCCCGGCACTGGACGGCAAGTCGGTGTATGAGCTGATCGAGGC
TGAAAAAGTCACGTATGCCGCAGGCGTGCCGACGGTGTGGCAGATGCTGCTCGGTCACCTCAAGCCGGCGGGCCTGAGGT
TTTCGACCCTGCGCCGCACCGTGATCGGTGGTTCGGCCTGTCCGCCCGCCATGATCAACGCTTTCAGGGATGACTACGGC
GTGGAGGTGCTTCACGCCTGGGGCATGACCGAGATGAGCCCGCTGGGCACGCTGTGCACGCTTAAAAACAAACATGACGC
GCTGTCTGACGACGAGAAAATGAAAATCCGCCTCAAGCAGGGGCGCGCCATTTTTGGCGTCGACATGAAAATCGTCGACG
AAGCCGGCAAGGACCTGCCCTGGGACGGCAAAACCTTTGGCGACCTGCATGTCAAAGGGCCGTGGATTGTGCGCGAGTAC
TTCAAGGGGGAGGGGGGCGACCCCTTGGTCAATGGCTGGTTCCCGACCGGTGACGTCGCGACTATCGATGCGGATGGCTA
CATGCAAATCACGGACCGCAGCAAGGATGTCATCAAGTCCGGTGGCGAGTGGATCAGCTCGATTGACGTGGAAAACATTG
CGGTGTCGCATCCGGCCGTGGCCATGGCTGCCTGTATCGGCGTTCCCCATCCCAAGTGGGATGAGCGGCCCATCATTGCG
GTGGTCAGGAAGCCGGGCATGGACGTCACGCGCGAAGAGCTGATCAGGTTCTACGACGGCAAGACGGCGAAATGGCAGAT
TCCCGATGACGTGGTGTTTGTCGATGCCATTCCGTTGGGCGCGACCGGCAAGATGCTCAAGACCAGGCTCCGTGAGCTGC
TGAAGGACTACAAGCTGCCTGGTATTTGA

Upstream 100 bases:

>100_bases
CGGATTTTCCCGGGGCGGCTCTGGTGGCGTGCGCGCTACCATGCAATCACGAGACATGTAGATTCATAAACGACCCGGCA
GGGCAAGAAAGGAAAAGACG

Downstream 100 bases:

>100_bases
CGACCACGTGGAAAGCCTGGCGCGCTGTCACCTAGGCGATATGTCCTGCGGACAACCCCTAGCGTTGGCAATTCGAATTA
CTTGTACGCTGCAAAGCTTG

Product: long-chain-fatty-acid--CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MLGLMQSQPLQISSLIEFAERHHGDAEIVSRRVEGDLHRYTYKDAACRARQVAQALDGLKLAFSDRVATLAWNGYRHFEL
YYGVSGSGRVLHTINPRLHPDQIAWIANHAEDQVLCFDLTFLPLVQAVHGKCPTIKHYIALCDADKLPADTGIPNLQSYE
RWIGGQTGDYAWPAFDENSASSMCYTSGTTGNPKAALFSHRSSILHAYAAALPDVMCISARDAILPVVPMFHVNAWGIPY
SAALAGAKLVFPGPALDGKSVYELIEAEKVTYAAGVPTVWQMLLGHLKPAGLRFSTLRRTVIGGSACPPAMINAFRDDYG
VEVLHAWGMTEMSPLGTLCTLKNKHDALSDDEKMKIRLKQGRAIFGVDMKIVDEAGKDLPWDGKTFGDLHVKGPWIVREY
FKGEGGDPLVNGWFPTGDVATIDADGYMQITDRSKDVIKSGGEWISSIDVENIAVSHPAVAMAACIGVPHPKWDERPIIA
VVRKPGMDVTREELIRFYDGKTAKWQIPDDVVFVDAIPLGATGKMLKTRLRELLKDYKLPGI

Sequences:

>Translated_542_residues
MLGLMQSQPLQISSLIEFAERHHGDAEIVSRRVEGDLHRYTYKDAACRARQVAQALDGLKLAFSDRVATLAWNGYRHFEL
YYGVSGSGRVLHTINPRLHPDQIAWIANHAEDQVLCFDLTFLPLVQAVHGKCPTIKHYIALCDADKLPADTGIPNLQSYE
RWIGGQTGDYAWPAFDENSASSMCYTSGTTGNPKAALFSHRSSILHAYAAALPDVMCISARDAILPVVPMFHVNAWGIPY
SAALAGAKLVFPGPALDGKSVYELIEAEKVTYAAGVPTVWQMLLGHLKPAGLRFSTLRRTVIGGSACPPAMINAFRDDYG
VEVLHAWGMTEMSPLGTLCTLKNKHDALSDDEKMKIRLKQGRAIFGVDMKIVDEAGKDLPWDGKTFGDLHVKGPWIVREY
FKGEGGDPLVNGWFPTGDVATIDADGYMQITDRSKDVIKSGGEWISSIDVENIAVSHPAVAMAACIGVPHPKWDERPIIA
VVRKPGMDVTREELIRFYDGKTAKWQIPDDVVFVDAIPLGATGKMLKTRLRELLKDYKLPGI
>Mature_542_residues
MLGLMQSQPLQISSLIEFAERHHGDAEIVSRRVEGDLHRYTYKDAACRARQVAQALDGLKLAFSDRVATLAWNGYRHFEL
YYGVSGSGRVLHTINPRLHPDQIAWIANHAEDQVLCFDLTFLPLVQAVHGKCPTIKHYIALCDADKLPADTGIPNLQSYE
RWIGGQTGDYAWPAFDENSASSMCYTSGTTGNPKAALFSHRSSILHAYAAALPDVMCISARDAILPVVPMFHVNAWGIPY
SAALAGAKLVFPGPALDGKSVYELIEAEKVTYAAGVPTVWQMLLGHLKPAGLRFSTLRRTVIGGSACPPAMINAFRDDYG
VEVLHAWGMTEMSPLGTLCTLKNKHDALSDDEKMKIRLKQGRAIFGVDMKIVDEAGKDLPWDGKTFGDLHVKGPWIVREY
FKGEGGDPLVNGWFPTGDVATIDADGYMQITDRSKDVIKSGGEWISSIDVENIAVSHPAVAMAACIGVPHPKWDERPIIA
VVRKPGMDVTREELIRFYDGKTAKWQIPDDVVFVDAIPLGATGKMLKTRLRELLKDYKLPGI

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=582, Percent_Identity=24.3986254295533, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI187761345, Length=543, Percent_Identity=26.5193370165746, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI187761343, Length=543, Percent_Identity=26.5193370165746, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI38505220, Length=429, Percent_Identity=24.009324009324, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI157311624, Length=551, Percent_Identity=22.8675136116152, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI157311622, Length=551, Percent_Identity=22.8675136116152, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI58082049, Length=551, Percent_Identity=23.2304900181488, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI42544132, Length=522, Percent_Identity=23.3716475095785, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI115511026, Length=369, Percent_Identity=25.4742547425474, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI122937307, Length=522, Percent_Identity=22.6053639846743, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI28416953, Length=444, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI221142682, Length=504, Percent_Identity=24.2063492063492, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1788107, Length=518, Percent_Identity=25.6756756756757, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI145693145, Length=364, Percent_Identity=29.1208791208791, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1786810, Length=402, Percent_Identity=27.363184079602, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789201, Length=363, Percent_Identity=24.7933884297521, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1788595, Length=330, Percent_Identity=25.7575757575758, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1790505, Length=384, Percent_Identity=24.7395833333333, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71994694, Length=552, Percent_Identity=25.3623188405797, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=552, Percent_Identity=25.3623188405797, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=552, Percent_Identity=25.3623188405797, Blast_Score=138, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17558820, Length=514, Percent_Identity=26.2645914396887, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32563687, Length=514, Percent_Identity=26.0700389105058, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17560308, Length=533, Percent_Identity=26.078799249531, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=430, Percent_Identity=25.8139534883721, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531443, Length=490, Percent_Identity=22.8571428571429, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=525, Percent_Identity=23.8095238095238, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17538037, Length=431, Percent_Identity=24.8259860788863, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71985884, Length=357, Percent_Identity=24.9299719887955, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=510, Percent_Identity=21.1764705882353, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=493, Percent_Identity=25.3549695740365, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24581924, Length=559, Percent_Identity=27.0125223613596, Blast_Score=148, Evalue=8e-36,
Organism=Drosophila melanogaster, GI18859661, Length=468, Percent_Identity=25.6410256410256, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355181, Length=377, Percent_Identity=27.8514588859416, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24648260, Length=506, Percent_Identity=22.1343873517787, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648257, Length=497, Percent_Identity=22.9376257545272, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653035, Length=534, Percent_Identity=21.3483146067416, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281365686, Length=254, Percent_Identity=25.9842519685039, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648253, Length=530, Percent_Identity=21.8867924528302, Blast_Score=83, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356441, Length=363, Percent_Identity=23.6914600550964, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648255, Length=530, Percent_Identity=21.8867924528302, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356947, Length=362, Percent_Identity=25.6906077348066, Blast_Score=76, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21358303, Length=329, Percent_Identity=23.7082066869301, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076582, Length=533, Percent_Identity=21.9512195121951, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI20130357, Length=366, Percent_Identity=22.4043715846995, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI62472339, Length=434, Percent_Identity=23.963133640553, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24667955, Length=434, Percent_Identity=23.963133640553, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24667959, Length=432, Percent_Identity=23.3796296296296, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 59622; Mature: 59622

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGLMQSQPLQISSLIEFAERHHGDAEIVSRRVEGDLHRYTYKDAACRARQVAQALDGLK
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCE
LAFSDRVATLAWNGYRHFELYYGVSGSGRVLHTINPRLHPDQIAWIANHAEDQVLCFDLT
EECCCCEEEEEECCEEEEEEEEEECCCCCEEEECCCCCCCCCEEEEECCCCCCEEEEECH
FLPLVQAVHGKCPTIKHYIALCDADKLPADTGIPNLQSYERWIGGQTGDYAWPAFDENSA
HHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
SSMCYTSGTTGNPKAALFSHRSSILHAYAAALPDVMCISARDAILPVVPMFHVNAWGIPY
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHCCCCEEEEECCCCCH
SAALAGAKLVFPGPALDGKSVYELIEAEKVTYAAGVPTVWQMLLGHLKPAGLRFSTLRRT
HHHHCCCEEEECCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCHHHHHHH
VIGGSACPPAMINAFRDDYGVEVLHAWGMTEMSPLGTLCTLKNKHDALSDDEKMKIRLKQ
HHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEC
GRAIFGVDMKIVDEAGKDLPWDGKTFGDLHVKGPWIVREYFKGEGGDPLVNGWFPTGDVA
CCEEEECCHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCEECCCCCCCEE
TIDADGYMQITDRSKDVIKSGGEWISSIDVENIAVSHPAVAMAACIGVPHPKWDERPIIA
EECCCCEEEECCCCHHHHHCCCHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCCCCCEEE
VVRKPGMDVTREELIRFYDGKTAKWQIPDDVVFVDAIPLGATGKMLKTRLRELLKDYKLP
EEECCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
GI
CC
>Mature Secondary Structure
MLGLMQSQPLQISSLIEFAERHHGDAEIVSRRVEGDLHRYTYKDAACRARQVAQALDGLK
CCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCE
LAFSDRVATLAWNGYRHFELYYGVSGSGRVLHTINPRLHPDQIAWIANHAEDQVLCFDLT
EECCCCEEEEEECCEEEEEEEEEECCCCCEEEECCCCCCCCCEEEEECCCCCCEEEEECH
FLPLVQAVHGKCPTIKHYIALCDADKLPADTGIPNLQSYERWIGGQTGDYAWPAFDENSA
HHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCC
SSMCYTSGTTGNPKAALFSHRSSILHAYAAALPDVMCISARDAILPVVPMFHVNAWGIPY
CCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHCCCCEEEEECCCCCH
SAALAGAKLVFPGPALDGKSVYELIEAEKVTYAAGVPTVWQMLLGHLKPAGLRFSTLRRT
HHHHCCCEEEECCCCCCHHHHHHHHHHHHEEEECCCHHHHHHHHHCCCCCCCCHHHHHHH
VIGGSACPPAMINAFRDDYGVEVLHAWGMTEMSPLGTLCTLKNKHDALSDDEKMKIRLKQ
HHCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEEEC
GRAIFGVDMKIVDEAGKDLPWDGKTFGDLHVKGPWIVREYFKGEGGDPLVNGWFPTGDVA
CCEEEECCHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCCEECCCCCCCEE
TIDADGYMQITDRSKDVIKSGGEWISSIDVENIAVSHPAVAMAACIGVPHPKWDERPIIA
EECCCCEEEECCCCHHHHHCCCHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCCCCCEEE
VVRKPGMDVTREELIRFYDGKTAKWQIPDDVVFVDAIPLGATGKMLKTRLRELLKDYKLP
EEECCCCCCCHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCC
GI
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]