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The map label for this gene is aes [H]

Identifier: 91788388

GI number: 91788388

Start: 2640555

End: 2641517

Strand: Reverse

Name: aes [H]

Synonym: Bpro_2525

Alternate gene names: 91788388

Gene position: 2641517-2640555 (Counterclockwise)

Preceding gene: 91788390

Following gene: 91788387

Centisome position: 50.8

GC content: 65.11

Gene sequence:

>963_bases
ATGCGGCATTCGGCTGACCCCCACAAGCTGCTGACCCCCGCCATGCGGGACGTGCTGCGCCGCATGGCCAAGGCCGGCCG
TCCGCCAATGCACACGCTAACGCCCCAGCAGGCGAGGGCGGCCTATGCTGCGGGTGCCGATGTGCTGGAGATACCTTCAC
CCCGGCTGGCCCGGGTGGAGGATTTCGCGCTGCCTGCCCGAGATGGCCACGCGATTGCAGCGAGGCTGTATGCGCCGTCC
AGCGATCACTTGCCGGTACTGGTGTACTTTCACGGCGGGGGCTTCACCATAGGCGGCATTGACACGCACGACGTGCTGTG
TCGCCAGCTCAGCCACCTTGCCGGTTGCGCTGTGATTTCGGTCGATTACCGCCTGGCGCCGGAGCACAAATTTCCGGTTG
CTGGCCATGACGCATGGGATGCGTTGCACTGGGTCGCCACCCACGGCGCCAGCCAAGGCATTGACAACACCCGTATCGCC
GTGGGAGGGGACAGCGCCGGCGGTACCCTGGCCGCGACGTGCGCGGTGCTTGCGCGGGATGCGGGGCTGCCGCTGGCCTT
GCAACTGCTGTTTTACCCCGGCTGTGCCGCCCACCAGAACACACCGTCGCACCAGAAATTTGCCACGGGCTTTGTGCTCG
AAGAAACTCATATCAGCTGGCTTTTCAGCCAATATGTCCGCAGCCCGGCTGACCGCGAGGACTGGCGCTTTGCGCCGCTG
CACACCCCTGACGTGGACGGGGTGGCCTCAGCCTGGGTAGGTTTGGCTGAATGCGACCCGCTGGTCGATGAAGGCGTGAT
GTATGCCGACAAGCTGCGTACTGCCGGTGTGCCCGTGGATCTGGAAATCTACCGCGGCGTTACGCATGAGTTCATCAAGA
TGGGCCGGGTCATCCCCGAGGCCCGGCGAGCTCACGCCGATGCCGCGCAGGCCATTCAGCGTGCGTTTGGATCGGCTCCT
TAG

Upstream 100 bases:

>100_bases
TTGACTTTACTTTACTGGCTGGACGCGCGCATGGGCCGCAAGCAAACCCTGATCTTGGCCGCCCTGGTGGTGGTGTGTTT
GACTGCCTCAAATAGTGATC

Downstream 100 bases:

>100_bases
GCGGAATTTCTGGGCATGAAACGAGCATGAAACGTCAAGACTTTCGTTTTTTTCACCGCCTGCGCGTGCGCTGGGTGGAA
GTGGACATGCAGAAAATCGT

Product: alpha/beta hydrolase fold-3 protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MRHSADPHKLLTPAMRDVLRRMAKAGRPPMHTLTPQQARAAYAAGADVLEIPSPRLARVEDFALPARDGHAIAARLYAPS
SDHLPVLVYFHGGGFTIGGIDTHDVLCRQLSHLAGCAVISVDYRLAPEHKFPVAGHDAWDALHWVATHGASQGIDNTRIA
VGGDSAGGTLAATCAVLARDAGLPLALQLLFYPGCAAHQNTPSHQKFATGFVLEETHISWLFSQYVRSPADREDWRFAPL
HTPDVDGVASAWVGLAECDPLVDEGVMYADKLRTAGVPVDLEIYRGVTHEFIKMGRVIPEARRAHADAAQAIQRAFGSAP

Sequences:

>Translated_320_residues
MRHSADPHKLLTPAMRDVLRRMAKAGRPPMHTLTPQQARAAYAAGADVLEIPSPRLARVEDFALPARDGHAIAARLYAPS
SDHLPVLVYFHGGGFTIGGIDTHDVLCRQLSHLAGCAVISVDYRLAPEHKFPVAGHDAWDALHWVATHGASQGIDNTRIA
VGGDSAGGTLAATCAVLARDAGLPLALQLLFYPGCAAHQNTPSHQKFATGFVLEETHISWLFSQYVRSPADREDWRFAPL
HTPDVDGVASAWVGLAECDPLVDEGVMYADKLRTAGVPVDLEIYRGVTHEFIKMGRVIPEARRAHADAAQAIQRAFGSAP
>Mature_320_residues
MRHSADPHKLLTPAMRDVLRRMAKAGRPPMHTLTPQQARAAYAAGADVLEIPSPRLARVEDFALPARDGHAIAARLYAPS
SDHLPVLVYFHGGGFTIGGIDTHDVLCRQLSHLAGCAVISVDYRLAPEHKFPVAGHDAWDALHWVATHGASQGIDNTRIA
VGGDSAGGTLAATCAVLARDAGLPLALQLLFYPGCAAHQNTPSHQKFATGFVLEETHISWLFSQYVRSPADREDWRFAPL
HTPDVDGVASAWVGLAECDPLVDEGVMYADKLRTAGVPVDLEIYRGVTHEFIKMGRVIPEARRAHADAAQAIQRAFGSAP

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=195, Percent_Identity=29.7435897435897, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI206597554, Length=153, Percent_Identity=36.6013071895425, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157041239, Length=192, Percent_Identity=32.2916666666667, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI68051721, Length=263, Percent_Identity=26.9961977186312, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI21328446, Length=106, Percent_Identity=39.622641509434, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI226423947, Length=271, Percent_Identity=26.1992619926199, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786682, Length=305, Percent_Identity=27.8688524590164, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17567059, Length=333, Percent_Identity=28.2282282282282, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71996133, Length=126, Percent_Identity=35.7142857142857, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540028, Length=141, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=141, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI115533410, Length=132, Percent_Identity=31.0606060606061, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115533412, Length=132, Percent_Identity=31.0606060606061, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34483; Mature: 34483

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHSADPHKLLTPAMRDVLRRMAKAGRPPMHTLTPQQARAAYAAGADVLEIPSPRLARVE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHH
DFALPARDGHAIAARLYAPSSDHLPVLVYFHGGGFTIGGIDTHDVLCRQLSHLAGCAVIS
HHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHHCEEEEE
VDYRLAPEHKFPVAGHDAWDALHWVATHGASQGIDNTRIAVGGDSAGGTLAATCAVLARD
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHC
AGLPLALQLLFYPGCAAHQNTPSHQKFATGFVLEETHISWLFSQYVRSPADREDWRFAPL
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHCEEEEHHHHHHHHHHHHCCCCCCCCCEECCC
HTPDVDGVASAWVGLAECDPLVDEGVMYADKLRTAGVPVDLEIYRGVTHEFIKMGRVIPE
CCCCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCHH
ARRAHADAAQAIQRAFGSAP
HHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRHSADPHKLLTPAMRDVLRRMAKAGRPPMHTLTPQQARAAYAAGADVLEIPSPRLARVE
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCHHHHH
DFALPARDGHAIAARLYAPSSDHLPVLVYFHGGGFTIGGIDTHDVLCRQLSHLAGCAVIS
HHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCEEECCCCHHHHHHHHHHHHHCEEEEE
VDYRLAPEHKFPVAGHDAWDALHWVATHGASQGIDNTRIAVGGDSAGGTLAATCAVLARD
EEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHC
AGLPLALQLLFYPGCAAHQNTPSHQKFATGFVLEETHISWLFSQYVRSPADREDWRFAPL
CCCHHHHHHHHCCCCCCCCCCCCHHHHHHCEEEEHHHHHHHHHHHHCCCCCCCCCEECCC
HTPDVDGVASAWVGLAECDPLVDEGVMYADKLRTAGVPVDLEIYRGVTHEFIKMGRVIPE
CCCCCCHHHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHCHH
ARRAHADAAQAIQRAFGSAP
HHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA