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The map label for this gene is holB [H]

Identifier: 91788381

GI number: 91788381

Start: 2632353

End: 2633492

Strand: Reverse

Name: holB [H]

Synonym: Bpro_2518

Alternate gene names: 91788381

Gene position: 2633492-2632353 (Counterclockwise)

Preceding gene: 91788382

Following gene: 91788380

Centisome position: 50.64

GC content: 66.49

Gene sequence:

>1140_bases
ATGAGTGAGGCCGCCAGCGAGATAAGTACAAGTACTGTGGTCAAAAAAGCGCCTCTGGCGCCCTGGCTGCAAACCCAGCT
GGAGAGCCTGCTGGCCCAGCGCGGCCACGCCTGGCTGCTCAGCGGCCCGTCGGGGCTGGGCCAGTTTGACCTGGCGCAGG
CACTGGCCCGCGCCTGGTTGTGCGAGCAGCCCACCCCACAGGGCGCTTGCGGTCACTGCGGCAGTTGCCACGCGGTCGAC
GTGCATACCCACGCCGACCTCTGCCTGCTGGTGCCTGAAACCCTGTCGCTCGCCCTGGGCTGGCCGCTGGACGAAAAAAC
CCAGGACGAGTTGGACAGCAAGAAACGCAAGCCCAGCAAGGAGATCAAGGTGGATGCGGCACGCGAGGCCGTGTCTTTCA
CGCAGTTCACGCGCTCGCGAGGCACGACCAAAGTCGTGCTGGTGTTTCCGGCCGAGCGCATGAACCACATCACGGCCAAC
ACCCTGCTCAAAACGCTGGAGGAGCCGCCCGGGCCCGTCAAATTCATTCTTGCGACCGATGCGGCCCACCAGCTGCTCCC
CACCATCCGCAGCCGCTGCCTTGGCCACACCATGCTCTGGCCCGATTTTGCGCAGGCTTCTGCCTGGCTCCAGCAGGAGG
CCGCCAGCCCGGGCGAGGGCGAAAGCAACCCAAAGAAGGGAGGGCCTGCGGCTGTACCTCCGGAGGCCGCAGATCTACAG
ACACTGCTGGTGGCGGCAGGGGGGCGTCCTGCCGATGCCCTGGCCTGGGCGCAAAACAGCGCCCCTGGTGAGGCGGCCCG
CCATTGGCAGGGCTTGCCCAAGGCGATGGCCCGCGGTGACGCCAGTGTGCTGGCCGATTGGTCGCCGGCCCAGGTCGTTG
ATGCCTTGCAGAAGCTTTGCCATGACGTTCTGGCTGTCAGGATGGGCGCGTCTCCGCGTTTTTTCAACGCCAAAGATCTG
CCGGAGCCGGTGGCCGGCAAACCTGGTAGCCCCCCCGCGCGCGTGCCGGGTCTTTATGCGCTTGCCAGCTGGTCCCGGGA
ACTGGCCGTCACCGCCCGCACTGTGGAGCATCCCTACAACCCGGGACTGATGCTGGAGTCCCTGGCGAGCCGCGCCCAAC
TGGCGCTCAGCTCGAAATAG

Upstream 100 bases:

>100_bases
AAGGCCATCCGGAACGCTTTGCCCGGATCAATGCCTCACAACCGCGCGATGTGGTCTGGCAGGCGGTGCGGCAGGTGTTT
GTGGCCAAAGGGTGGCTGGC

Downstream 100 bases:

>100_bases
GTCCCTCAGGGCGGCAATTGCAGGCAGACTCCTGTTCAAGCCTGATTGATAGGGACCTTGAACAACAGTTCACCGGTTTT
ATTTGTGCCTGCCAGCGTGC

Product: DNA-directed DNA polymerase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSEAASEISTSTVVKKAPLAPWLQTQLESLLAQRGHAWLLSGPSGLGQFDLAQALARAWLCEQPTPQGACGHCGSCHAVD
VHTHADLCLLVPETLSLALGWPLDEKTQDELDSKKRKPSKEIKVDAAREAVSFTQFTRSRGTTKVVLVFPAERMNHITAN
TLLKTLEEPPGPVKFILATDAAHQLLPTIRSRCLGHTMLWPDFAQASAWLQQEAASPGEGESNPKKGGPAAVPPEAADLQ
TLLVAAGGRPADALAWAQNSAPGEAARHWQGLPKAMARGDASVLADWSPAQVVDALQKLCHDVLAVRMGASPRFFNAKDL
PEPVAGKPGSPPARVPGLYALASWSRELAVTARTVEHPYNPGLMLESLASRAQLALSSK

Sequences:

>Translated_379_residues
MSEAASEISTSTVVKKAPLAPWLQTQLESLLAQRGHAWLLSGPSGLGQFDLAQALARAWLCEQPTPQGACGHCGSCHAVD
VHTHADLCLLVPETLSLALGWPLDEKTQDELDSKKRKPSKEIKVDAAREAVSFTQFTRSRGTTKVVLVFPAERMNHITAN
TLLKTLEEPPGPVKFILATDAAHQLLPTIRSRCLGHTMLWPDFAQASAWLQQEAASPGEGESNPKKGGPAAVPPEAADLQ
TLLVAAGGRPADALAWAQNSAPGEAARHWQGLPKAMARGDASVLADWSPAQVVDALQKLCHDVLAVRMGASPRFFNAKDL
PEPVAGKPGSPPARVPGLYALASWSRELAVTARTVEHPYNPGLMLESLASRAQLALSSK
>Mature_378_residues
SEAASEISTSTVVKKAPLAPWLQTQLESLLAQRGHAWLLSGPSGLGQFDLAQALARAWLCEQPTPQGACGHCGSCHAVDV
HTHADLCLLVPETLSLALGWPLDEKTQDELDSKKRKPSKEIKVDAAREAVSFTQFTRSRGTTKVVLVFPAERMNHITANT
LLKTLEEPPGPVKFILATDAAHQLLPTIRSRCLGHTMLWPDFAQASAWLQQEAASPGEGESNPKKGGPAAVPPEAADLQT
LLVAAGGRPADALAWAQNSAPGEAARHWQGLPKAMARGDASVLADWSPAQVVDALQKLCHDVLAVRMGASPRFFNAKDLP
EPVAGKPGSPPARVPGLYALASWSRELAVTARTVEHPYNPGLMLESLASRAQLALSSK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=199, Percent_Identity=36.1809045226131, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786676, Length=182, Percent_Identity=31.3186813186813, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40374; Mature: 40243

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAASEISTSTVVKKAPLAPWLQTQLESLLAQRGHAWLLSGPSGLGQFDLAQALARAWL
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
CEQPTPQGACGHCGSCHAVDVHTHADLCLLVPETLSLALGWPLDEKTQDELDSKKRKPSK
CCCCCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHCCCCH
EIKVDAAREAVSFTQFTRSRGTTKVVLVFPAERMNHITANTLLKTLEEPPGPVKFILATD
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
AAHQLLPTIRSRCLGHTMLWPDFAQASAWLQQEAASPGEGESNPKKGGPAAVPPEAADLQ
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
TLLVAAGGRPADALAWAQNSAPGEAARHWQGLPKAMARGDASVLADWSPAQVVDALQKLC
HHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCCHHHHHCCCCCEECCCCHHHHHHHHHHHH
HDVLAVRMGASPRFFNAKDLPEPVAGKPGSPPARVPGLYALASWSRELAVTARTVEHPYN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCC
PGLMLESLASRAQLALSSK
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEAASEISTSTVVKKAPLAPWLQTQLESLLAQRGHAWLLSGPSGLGQFDLAQALARAWL
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHH
CEQPTPQGACGHCGSCHAVDVHTHADLCLLVPETLSLALGWPLDEKTQDELDSKKRKPSK
CCCCCCCCCCCCCCCCCEEEECCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHCCCCH
EIKVDAAREAVSFTQFTRSRGTTKVVLVFPAERMNHITANTLLKTLEEPPGPVKFILATD
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
AAHQLLPTIRSRCLGHTMLWPDFAQASAWLQQEAASPGEGESNPKKGGPAAVPPEAADLQ
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
TLLVAAGGRPADALAWAQNSAPGEAARHWQGLPKAMARGDASVLADWSPAQVVDALQKLC
HHHHHCCCCCHHHHHHCCCCCCHHHHHHHHCCHHHHHCCCCCEECCCCHHHHHHHHHHHH
HDVLAVRMGASPRFFNAKDLPEPVAGKPGSPPARVPGLYALASWSRELAVTARTVEHPYN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCC
PGLMLESLASRAQLALSSK
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]