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The map label for this gene is cysW [H]

Identifier: 91788245

GI number: 91788245

Start: 2471657

End: 2472676

Strand: Direct

Name: cysW [H]

Synonym: Bpro_2378

Alternate gene names: 91788245

Gene position: 2471657-2472676 (Clockwise)

Preceding gene: 91788244

Following gene: 91788246

Centisome position: 47.53

GC content: 62.55

Gene sequence:

>1020_bases
ATGAGCGATAAACAAGCCCCCACACTTGTCGCTACGCGAGCCTCCGGCGTTGCGCCGCCCCTTGCCGACAGAAAAGGGGC
TGTGCTTGCTCGGGGCGGTCCTTCGCGGCGCACTCCTGCCCAACCGGTTCGCCGCGTCACGGCCGGAACGACTGAGTCGC
CCTGGGTGCGTTACACGCTGATCACGGGTGCTCTTTTATTCGTGTTCCTGTTTCTCGTGCTGCCGCTGGCCGCCGTGTTC
ACCGAAGCCTTGCGCAAGGGCTTGGGTGCCTACATCGCCGCGCTGCAGGAACCCGACGCCTGGTCGGCCATCAAGCTCAC
CTTGATTGCCGCCGCCGTTGCCGTACCGCTGAATCTGGTGTTTGGCGTAGCCGCAGCCTGGGCCATTGCCAAGTACGAGT
TTCGCGGCAAGTCGTTTTTGACGACCCTGGTGGACCTGCCGTTTTCCGTCTCGCCCGTGGTGGCAGGCCTGATCTATGTG
CTGCTGTTTGGCGCCCAGGGCTGGTTTGGCCCCTGGCTGCAGGCGCATGACATCAAGATCATTTTTGCCGTGCCCGGCAT
TGTGCTGGCGACCGTGTTCGTGACCTTTCCCTTCATTGCGCGCGAGCTGATTCCACTGATGCAGGCGCAGGGCAACGACG
AAGAGCAGGCAGCCATTGTGCTGGGCGCCACCGGCTGGCAAACCTTCTGGCATGTCACGCTGCCCAACATCAAGTGGGGT
TTGATCTACGGCGTGATCCTGTGCAATGCGCGGGCCATGGGCGAGTTTGGCGCGGTCTCCGTGGTGTCGGGCCACATCCG
CGGGCAAACCAACACCTTGCCGCTGCATGTGGAAATTTTGTACAACGAATACCAGTCGGTGGCCGCCTTTGCCGTGGCTT
CGCTGCTGGCCATTCTGGCGCTGGTCACGCTGGCCATCAAGTCGGTGGCGGAATGGCGCCACGAAGCAGAAATGAAGGCT
GCGGCTGATTTGCCGCCTGAACGCCCCGTGTTATCCCCTCTCCCCCTGGGAGAGGGCTAG

Upstream 100 bases:

>100_bases
CTACCGCAGTGGCCGTGGTGATGCTGGTGCTGTCATTCATCCTGCTGCTGGTCATCAACGCATTACAGGCCTGGCAGCGC
CGCAACGCAGGAGCGCCGGC

Downstream 100 bases:

>100_bases
GGTGAGGGCGCGGCCCCACCTCGTAGCCAACGCCGGCCCTCACCCCAACCCTCTCCCAGTGGGAGAGGGAGAAAGAGAAA
GAGAAAGAAATGAGCATCGA

Product: Sulfate ABC transporter permease CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSDKQAPTLVATRASGVAPPLADRKGAVLARGGPSRRTPAQPVRRVTAGTTESPWVRYTLITGALLFVFLFLVLPLAAVF
TEALRKGLGAYIAALQEPDAWSAIKLTLIAAAVAVPLNLVFGVAAAWAIAKYEFRGKSFLTTLVDLPFSVSPVVAGLIYV
LLFGAQGWFGPWLQAHDIKIIFAVPGIVLATVFVTFPFIARELIPLMQAQGNDEEQAAIVLGATGWQTFWHVTLPNIKWG
LIYGVILCNARAMGEFGAVSVVSGHIRGQTNTLPLHVEILYNEYQSVAAFAVASLLAILALVTLAIKSVAEWRHEAEMKA
AADLPPERPVLSPLPLGEG

Sequences:

>Translated_339_residues
MSDKQAPTLVATRASGVAPPLADRKGAVLARGGPSRRTPAQPVRRVTAGTTESPWVRYTLITGALLFVFLFLVLPLAAVF
TEALRKGLGAYIAALQEPDAWSAIKLTLIAAAVAVPLNLVFGVAAAWAIAKYEFRGKSFLTTLVDLPFSVSPVVAGLIYV
LLFGAQGWFGPWLQAHDIKIIFAVPGIVLATVFVTFPFIARELIPLMQAQGNDEEQAAIVLGATGWQTFWHVTLPNIKWG
LIYGVILCNARAMGEFGAVSVVSGHIRGQTNTLPLHVEILYNEYQSVAAFAVASLLAILALVTLAIKSVAEWRHEAEMKA
AADLPPERPVLSPLPLGEG
>Mature_338_residues
SDKQAPTLVATRASGVAPPLADRKGAVLARGGPSRRTPAQPVRRVTAGTTESPWVRYTLITGALLFVFLFLVLPLAAVFT
EALRKGLGAYIAALQEPDAWSAIKLTLIAAAVAVPLNLVFGVAAAWAIAKYEFRGKSFLTTLVDLPFSVSPVVAGLIYVL
LFGAQGWFGPWLQAHDIKIIFAVPGIVLATVFVTFPFIARELIPLMQAQGNDEEQAAIVLGATGWQTFWHVTLPNIKWGL
IYGVILCNARAMGEFGAVSVVSGHIRGQTNTLPLHVEILYNEYQSVAAFAVASLLAILALVTLAIKSVAEWRHEAEMKAA
ADLPPERPVLSPLPLGEG

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=286, Percent_Identity=48.2517482517483, Blast_Score=285, Evalue=2e-78,
Organism=Escherichia coli, GI1788764, Length=206, Percent_Identity=38.8349514563107, Blast_Score=148, Evalue=4e-37,
Organism=Escherichia coli, GI1786980, Length=221, Percent_Identity=33.4841628959276, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36267; Mature: 36136

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKQAPTLVATRASGVAPPLADRKGAVLARGGPSRRTPAQPVRRVTAGTTESPWVRYTL
CCCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCEEEHHH
ITGALLFVFLFLVLPLAAVFTEALRKGLGAYIAALQEPDAWSAIKLTLIAAAVAVPLNLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FGVAAAWAIAKYEFRGKSFLTTLVDLPFSVSPVVAGLIYVLLFGAQGWFGPWLQAHDIKI
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
IFAVPGIVLATVFVTFPFIARELIPLMQAQGNDEEQAAIVLGATGWQTFWHVTLPNIKWG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCHHH
LIYGVILCNARAMGEFGAVSVVSGHIRGQTNTLPLHVEILYNEYQSVAAFAVASLLAILA
HHHHHHHHCCHHCCCCCCHHEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHH
LVTLAIKSVAEWRHEAEMKAAADLPPERPVLSPLPLGEG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDKQAPTLVATRASGVAPPLADRKGAVLARGGPSRRTPAQPVRRVTAGTTESPWVRYTL
CCCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCCEEEHHH
ITGALLFVFLFLVLPLAAVFTEALRKGLGAYIAALQEPDAWSAIKLTLIAAAVAVPLNLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
FGVAAAWAIAKYEFRGKSFLTTLVDLPFSVSPVVAGLIYVLLFGAQGWFGPWLQAHDIKI
HHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEE
IFAVPGIVLATVFVTFPFIARELIPLMQAQGNDEEQAAIVLGATGWQTFWHVTLPNIKWG
EEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEEEEEECCCCHHH
LIYGVILCNARAMGEFGAVSVVSGHIRGQTNTLPLHVEILYNEYQSVAAFAVASLLAILA
HHHHHHHHCCHHCCCCCCHHEEECCCCCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHH
LVTLAIKSVAEWRHEAEMKAAADLPPERPVLSPLPLGEG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]