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The map label for this gene is cysT [H]

Identifier: 91788244

GI number: 91788244

Start: 2470767

End: 2471660

Strand: Direct

Name: cysT [H]

Synonym: Bpro_2377

Alternate gene names: 91788244

Gene position: 2470767-2471660 (Clockwise)

Preceding gene: 91788243

Following gene: 91788245

Centisome position: 47.51

GC content: 62.64

Gene sequence:

>894_bases
ATGACTTCCCTGTCTTCTGTTGCACCGCCCGCCGCGGTGCTGCCGGGCTCGCCTGCGGGCGGCAAACGGGCTGCCCGGCG
GGTGTTGCCCGGCTTTTACCTCACGCTGGGCTACACGGTTTTGTACCTCAGCCTGATCGTGCTGATCCCGATCTCGGCGC
TGTTCTTCAAAACGTTCACGCTCACCCTGGATCAGTTCTGGGTGGCCGTGACCTCGCCGCGCGTGCTGGCCTCCTACCGC
CTCACGTTTGGCGCGTCGCTGATCGCGGCGCTCGTCAACCTGGTGTTCGGCCTGCTGGTGGCCTGGGTGCTGGTGCGCTA
CACGTTTCCCGGCAAAAAACTGGTTGATGCGCTGGTTGATTTGCCCTTTGCGCTGCCCACGGCCGTGGCCGGCATTTCGC
TCACAGCGCTGCTGGCGGGCAACGGCTGGCTCGGCCAGTACCTGGAGCCCATGGGCATCAAGCTGGCCTTCACGCCGGCC
GGCGTGGTGATTGCGCTGATTTTTGTGGGTCTTCCGTTTGTCGTGCGCACGGTGCAGCCGGTGCTGGAAGATGCGGAGAA
AGAACTCGAAGAAGCCGCAACGTCATTGGGCGCCACACGCCTGCAGATTTTCACCAGGGTCATCCTGCCGCACATCATGC
CGGCGCTGCTGACGGGCTTTGCCATGGCCTTTGCGCGGGCGATTGGCGAGTACGGATCGGTTATCTTTATTGCCGGCAAC
ATGCCCATGATTTCGGAGATCACGCCGCTCATCATCATCGGCAAGCTCGAGCAGTACGACTATGCTGGTGCTACCGCAGT
GGCCGTGGTGATGCTGGTGCTGTCATTCATCCTGCTGCTGGTCATCAACGCATTACAGGCCTGGCAGCGCCGCAACGCAG
GAGCGCCGGCATGA

Upstream 100 bases:

>100_bases
TGCAGGAGTTGTTTGGCTCCCTGAGCGAAGCGCAAAAAGTCCACTTCAATGACGGCGGACAGTTCGACAAGCTTTACACC
GTCAAGTAAGCCTCCACGCC

Downstream 100 bases:

>100_bases
GCGATAAACAAGCCCCCACACTTGTCGCTACGCGAGCCTCCGGCGTTGCGCCGCCCCTTGCCGACAGAAAAGGGGCTGTG
CTTGCTCGGGGCGGTCCTTC

Product: Sulfate ABC transporter permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTSLSSVAPPAAVLPGSPAGGKRAARRVLPGFYLTLGYTVLYLSLIVLIPISALFFKTFTLTLDQFWVAVTSPRVLASYR
LTFGASLIAALVNLVFGLLVAWVLVRYTFPGKKLVDALVDLPFALPTAVAGISLTALLAGNGWLGQYLEPMGIKLAFTPA
GVVIALIFVGLPFVVRTVQPVLEDAEKELEEAATSLGATRLQIFTRVILPHIMPALLTGFAMAFARAIGEYGSVIFIAGN
MPMISEITPLIIIGKLEQYDYAGATAVAVVMLVLSFILLLVINALQAWQRRNAGAPA

Sequences:

>Translated_297_residues
MTSLSSVAPPAAVLPGSPAGGKRAARRVLPGFYLTLGYTVLYLSLIVLIPISALFFKTFTLTLDQFWVAVTSPRVLASYR
LTFGASLIAALVNLVFGLLVAWVLVRYTFPGKKLVDALVDLPFALPTAVAGISLTALLAGNGWLGQYLEPMGIKLAFTPA
GVVIALIFVGLPFVVRTVQPVLEDAEKELEEAATSLGATRLQIFTRVILPHIMPALLTGFAMAFARAIGEYGSVIFIAGN
MPMISEITPLIIIGKLEQYDYAGATAVAVVMLVLSFILLLVINALQAWQRRNAGAPA
>Mature_296_residues
TSLSSVAPPAAVLPGSPAGGKRAARRVLPGFYLTLGYTVLYLSLIVLIPISALFFKTFTLTLDQFWVAVTSPRVLASYRL
TFGASLIAALVNLVFGLLVAWVLVRYTFPGKKLVDALVDLPFALPTAVAGISLTALLAGNGWLGQYLEPMGIKLAFTPAG
VVIALIFVGLPFVVRTVQPVLEDAEKELEEAATSLGATRLQIFTRVILPHIMPALLTGFAMAFARAIGEYGSVIFIAGNM
PMISEITPLIIIGKLEQYDYAGATAVAVVMLVLSFILLLVINALQAWQRRNAGAPA

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=272, Percent_Identity=49.6323529411765, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI87082099, Length=276, Percent_Identity=35.1449275362319, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1786980, Length=216, Percent_Identity=34.7222222222222, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1787369, Length=181, Percent_Identity=30.939226519337, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786254, Length=223, Percent_Identity=25.5605381165919, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787368, Length=184, Percent_Identity=31.5217391304348, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31646; Mature: 31515

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSLSSVAPPAAVLPGSPAGGKRAARRVLPGFYLTLGYTVLYLSLIVLIPISALFFKTFT
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLDQFWVAVTSPRVLASYRLTFGASLIAALVNLVFGLLVAWVLVRYTFPGKKLVDALVD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LPFALPTAVAGISLTALLAGNGWLGQYLEPMGIKLAFTPAGVVIALIFVGLPFVVRTVQP
CCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
VLEDAEKELEEAATSLGATRLQIFTRVILPHIMPALLTGFAMAFARAIGEYGSVIFIAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
MPMISEITPLIIIGKLEQYDYAGATAVAVVMLVLSFILLLVINALQAWQRRNAGAPA
CCHHHHCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TSLSSVAPPAAVLPGSPAGGKRAARRVLPGFYLTLGYTVLYLSLIVLIPISALFFKTFT
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTLDQFWVAVTSPRVLASYRLTFGASLIAALVNLVFGLLVAWVLVRYTFPGKKLVDALVD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LPFALPTAVAGISLTALLAGNGWLGQYLEPMGIKLAFTPAGVVIALIFVGLPFVVRTVQP
CCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHH
VLEDAEKELEEAATSLGATRLQIFTRVILPHIMPALLTGFAMAFARAIGEYGSVIFIAGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
MPMISEITPLIIIGKLEQYDYAGATAVAVVMLVLSFILLLVINALQAWQRRNAGAPA
CCHHHHCCCEEEEECHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]