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The map label for this gene is sbp [H]

Identifier: 91788243

GI number: 91788243

Start: 2469763

End: 2470755

Strand: Direct

Name: sbp [H]

Synonym: Bpro_2376

Alternate gene names: 91788243

Gene position: 2469763-2470755 (Clockwise)

Preceding gene: 91788242

Following gene: 91788244

Centisome position: 47.49

GC content: 59.92

Gene sequence:

>993_bases
ATGACTTCAAAATTCAAAATCGCTCTTGCAACCCTGGCGCTGGTCACGGGCGGTATCTCCTCGGCGCAAACCTTGCTCAA
CGGTTCGTACGACGTGGCGCGCGAGTTTTACAAGGACTACAACGCGGCCTTCATTGCCCACTACAAGAAAACCACCGGCA
AGGATGTGAAGGTCGACCAGTCGCACGGCGGCTCCAGCGCCATTGCACGCTCCGTGGCCGATGGCCTGGACGCTGACGTG
GTGACCATGAACACCAGCACCGACGTGGAGTTTCTGGCCAATGCCGGTGTCGTGGCCAAAGACTGGGCCAAGCGCTTTCC
GGACAATGCCTCGCCCACCACCTCCACCATGCTGTTCCTGGTGCGCAACGGCAACCCCAAGGGCATCAAGGACTGGGATG
ACCTGGTCAAGCCCGGCATCCAGGTGATCGTGGTCAACCCCAAAACCGGCGGCAATGGCCGCTACGCCTACCTGGCGGCC
TGGGGCTATGCGAAGAAGAAGGGCGCCAGCGATGCGCAGGCCGCCGAGTTTGTCGGCAAGCTGTACAAAAACGTGCCCGT
GCTGGGCAAGGGTGGTCGCGATGCCACCACGGTGTTCCTGCAGCGCAATATCGGCGATGTGCTAATCACCTTCGAATCGG
AAGTGCTGTCGGTGAACAAGGAGTTTGGCGAAGGCAAGGTGGACGCGATTTACCCGTCCATCAGCATCCTGGCTGAAAAC
CCGGTGGCGGTGGTCGAACGCACTGTGGCCAAGAAGGGCACGGCGGACCTTGCCAAGGCTTATCTCAACTACCTCTACTC
GGACGAAGGCCAGGAAATTGCCGCCCGCCACGCGCTGCGCCCGCGCTCGCAGGCCGTGCTCAAAAAGTACGCCAACACCT
TCAAGCCCGTGCAGCTGTTCACCGTGCAGGAGTTGTTTGGCTCCCTGAGCGAAGCGCAAAAAGTCCACTTCAATGACGGC
GGACAGTTCGACAAGCTTTACACCGTCAAGTAA

Upstream 100 bases:

>100_bases
ATGCGCCCCTGACGGCCCACCCTGGTTTTTCATCCTCATCTTCAACACCTCCTTCACCTTCTTCTTCTTCTTCCTCCTCT
CTTGGATCCCTGGAAACGGC

Downstream 100 bases:

>100_bases
GCCTCCACGCCATGACTTCCCTGTCTTCTGTTGCACCGCCCGCCGCGGTGCTGCCGGGCTCGCCTGCGGGCGGCAAACGG
GCTGCCCGGCGGGTGTTGCC

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MTSKFKIALATLALVTGGISSAQTLLNGSYDVAREFYKDYNAAFIAHYKKTTGKDVKVDQSHGGSSAIARSVADGLDADV
VTMNTSTDVEFLANAGVVAKDWAKRFPDNASPTTSTMLFLVRNGNPKGIKDWDDLVKPGIQVIVVNPKTGGNGRYAYLAA
WGYAKKKGASDAQAAEFVGKLYKNVPVLGKGGRDATTVFLQRNIGDVLITFESEVLSVNKEFGEGKVDAIYPSISILAEN
PVAVVERTVAKKGTADLAKAYLNYLYSDEGQEIAARHALRPRSQAVLKKYANTFKPVQLFTVQELFGSLSEAQKVHFNDG
GQFDKLYTVK

Sequences:

>Translated_330_residues
MTSKFKIALATLALVTGGISSAQTLLNGSYDVAREFYKDYNAAFIAHYKKTTGKDVKVDQSHGGSSAIARSVADGLDADV
VTMNTSTDVEFLANAGVVAKDWAKRFPDNASPTTSTMLFLVRNGNPKGIKDWDDLVKPGIQVIVVNPKTGGNGRYAYLAA
WGYAKKKGASDAQAAEFVGKLYKNVPVLGKGGRDATTVFLQRNIGDVLITFESEVLSVNKEFGEGKVDAIYPSISILAEN
PVAVVERTVAKKGTADLAKAYLNYLYSDEGQEIAARHALRPRSQAVLKKYANTFKPVQLFTVQELFGSLSEAQKVHFNDG
GQFDKLYTVK
>Mature_329_residues
TSKFKIALATLALVTGGISSAQTLLNGSYDVAREFYKDYNAAFIAHYKKTTGKDVKVDQSHGGSSAIARSVADGLDADVV
TMNTSTDVEFLANAGVVAKDWAKRFPDNASPTTSTMLFLVRNGNPKGIKDWDDLVKPGIQVIVVNPKTGGNGRYAYLAAW
GYAKKKGASDAQAAEFVGKLYKNVPVLGKGGRDATTVFLQRNIGDVLITFESEVLSVNKEFGEGKVDAIYPSISILAENP
VAVVERTVAKKGTADLAKAYLNYLYSDEGQEIAARHALRPRSQAVLKKYANTFKPVQLFTVQELFGSLSEAQKVHFNDGG
QFDKLYTVK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=325, Percent_Identity=51.3846153846154, Blast_Score=334, Evalue=5e-93,
Organism=Escherichia coli, GI1788765, Length=312, Percent_Identity=46.1538461538462, Blast_Score=274, Evalue=7e-75,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 35696; Mature: 35565

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKFKIALATLALVTGGISSAQTLLNGSYDVAREFYKDYNAAFIAHYKKTTGKDVKVDQ
CCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEEHHCCCCCEEEEC
SHGGSSAIARSVADGLDADVVTMNTSTDVEFLANAGVVAKDWAKRFPDNASPTTSTMLFL
CCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEE
VRNGNPKGIKDWDDLVKPGIQVIVVNPKTGGNGRYAYLAAWGYAKKKGASDAQAAEFVGK
EECCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCEEEEEEECCHHHCCCCHHHHHHHHHH
LYKNVPVLGKGGRDATTVFLQRNIGDVLITFESEVLSVNKEFGEGKVDAIYPSISILAEN
HHHCCCCCCCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHCCCCCEEEECCCEEEEECC
PVAVVERTVAKKGTADLAKAYLNYLYSDEGQEIAARHALRPRSQAVLKKYANTFKPVQLF
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
TVQELFGSLSEAQKVHFNDGGQFDKLYTVK
EHHHHHHHHHHHCEEEECCCCCCCEEEECC
>Mature Secondary Structure 
TSKFKIALATLALVTGGISSAQTLLNGSYDVAREFYKDYNAAFIAHYKKTTGKDVKVDQ
CCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEEHHCCCCCEEEEC
SHGGSSAIARSVADGLDADVVTMNTSTDVEFLANAGVVAKDWAKRFPDNASPTTSTMLFL
CCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEE
VRNGNPKGIKDWDDLVKPGIQVIVVNPKTGGNGRYAYLAAWGYAKKKGASDAQAAEFVGK
EECCCCCCCCCHHHHHCCCEEEEEECCCCCCCCCEEEEEEECCHHHCCCCHHHHHHHHHH
LYKNVPVLGKGGRDATTVFLQRNIGDVLITFESEVLSVNKEFGEGKVDAIYPSISILAEN
HHHCCCCCCCCCCCCEEEEEECCCCCEEEEECHHHHHHHHHCCCCCEEEECCCEEEEECC
PVAVVERTVAKKGTADLAKAYLNYLYSDEGQEIAARHALRPRSQAVLKKYANTFKPVQLF
CHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
TVQELFGSLSEAQKVHFNDGGQFDKLYTVK
EHHHHHHHHHHHCEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]