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The map label for this gene is cysNC [H]

Identifier: 91788204

GI number: 91788204

Start: 2432631

End: 2433947

Strand: Reverse

Name: cysNC [H]

Synonym: Bpro_2335

Alternate gene names: 91788204

Gene position: 2433947-2432631 (Counterclockwise)

Preceding gene: 91788205

Following gene: 91788203

Centisome position: 46.8

GC content: 63.78

Gene sequence:

>1317_bases
ATGAGCACGCCCACTGTTGAAACCGTCAGCCTGACCACAGCTGCAACTGCCCAGGACACGCGTGCCGCCCTGCGTTTTGT
CACCTGCGGCAGCGTCGATGATGGCAAGAGCACCCTGATTGGCCGCCTGCTGGTCGACAGCCGTGCCGTGCTGCAGGATC
ACCTGGCCGGCGTGCAGCGCGGCGGTGAGACTGACCTGGCCCTGCTGACCGATGGCCTGTCTGCCGAGCGCGAACAGGGC
ATCACCATTGACGTAGCCTACCGGTATTTCTCTACCGAGAGCCGCAAGTTCATCATTGCCGATGCCCCGGGCCACGAGCA
ATACACCCGCAACATGGTCACTGCGGCATCGAGCGCCGATGCCGCAGTGGTGCTGGTTGATGCCACCAAACTGACTTGGG
AGGCGCCTTCGCCCGAGCTGCTGCCGCAAACCCGCCGCCATTCGCTGCTGGTCGATCTGCTGCGCGTGCCGTCCATCGTC
TTTGCCATCAACAAGCTCGACGCGGTTGCCAACCCGGCCCTGGCCTTCGCCCATATCAGCGCGGCCTTGAGCCAGTTTGC
CAAGGAGGCGCAGATTCCGGTCAAGGCCATCGTGCCGGTGTCGGCCCTGATTGGCTACAACGTGGTGGATGCCCACCCCG
GCTGGTGCGATTACCAGGGCCCCAGCCTGCTGGAGATTCTGGAGCACCTGCCGGCCACACCGGCCGATACGTCGGAGGCT
TTTGCGTTTCCGGTGCAGTGGGTGGAGAAGTTTTCATCCAGCGCGGACACCTCGCAGGGACGACGTATCTTCTGGGGTCG
CATTGCCAGCGGTGCAGTACAACCGGGCCAGCAGATTACCGTGCTGCCGGGTGGGAAAAGCGCCACAGTGGCCCAAGTAC
TGGACCATACGCGCCAACCCAAGGCTGTATTGGCCGGGCACAGCGCCGGCATCATCCTGGACCGCGAACTGGACGTGTCA
CGCGGTGACTGGCTGCTGGCGGAGGGCGGATTCGAGCCACCGCTGGGCGTGCGCGAAATCACCGCGGAAGTTGCCTGGAT
GGACGACGAACCCCTGGTACCGGGCCGCATGTACTGGGCCTTGCAGGGCCACCGCTGGGTCAAGGCCAAAATCAAGCGTA
TCGTCCACCAGGTAAACATCAATACCCTGGAGACCCACGACGCAACACACCTGGACGCGAACGCCATCGGTGTCGTCGAG
TTGGCCCTGCTGGAGCCGATTCCGGCACGGCCTTACCTGCAGTCCCGCATCCTCGGCTCGCTGGTACTGGTGGACACGGC
GTCGCACAAAACATCCGGCGCCGCACTGGTTCTTTAA

Upstream 100 bases:

>100_bases
AAACGCTGGCCGCCGATGTCAGCGAGCGCGGCGCCACCCGCATGGACGACAAAACCTCCGATGCCTCCATGGAAAAGCGG
AAAAAAGAAGGATATTTCTG

Downstream 100 bases:

>100_bases
GCTGCCAAGGCCCTTTCAATTCACTGCTTCAACGCTGCTACTGGAAACCTCTTAAAATCAAGGGTTTCCACCGATCAGCC
AATACAACATGACACACATC

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: Sulfate adenylyltransferase subunit 1; ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSTPTVETVSLTTAATAQDTRAALRFVTCGSVDDGKSTLIGRLLVDSRAVLQDHLAGVQRGGETDLALLTDGLSAEREQG
ITIDVAYRYFSTESRKFIIADAPGHEQYTRNMVTAASSADAAVVLVDATKLTWEAPSPELLPQTRRHSLLVDLLRVPSIV
FAINKLDAVANPALAFAHISAALSQFAKEAQIPVKAIVPVSALIGYNVVDAHPGWCDYQGPSLLEILEHLPATPADTSEA
FAFPVQWVEKFSSSADTSQGRRIFWGRIASGAVQPGQQITVLPGGKSATVAQVLDHTRQPKAVLAGHSAGIILDRELDVS
RGDWLLAEGGFEPPLGVREITAEVAWMDDEPLVPGRMYWALQGHRWVKAKIKRIVHQVNINTLETHDATHLDANAIGVVE
LALLEPIPARPYLQSRILGSLVLVDTASHKTSGAALVL

Sequences:

>Translated_438_residues
MSTPTVETVSLTTAATAQDTRAALRFVTCGSVDDGKSTLIGRLLVDSRAVLQDHLAGVQRGGETDLALLTDGLSAEREQG
ITIDVAYRYFSTESRKFIIADAPGHEQYTRNMVTAASSADAAVVLVDATKLTWEAPSPELLPQTRRHSLLVDLLRVPSIV
FAINKLDAVANPALAFAHISAALSQFAKEAQIPVKAIVPVSALIGYNVVDAHPGWCDYQGPSLLEILEHLPATPADTSEA
FAFPVQWVEKFSSSADTSQGRRIFWGRIASGAVQPGQQITVLPGGKSATVAQVLDHTRQPKAVLAGHSAGIILDRELDVS
RGDWLLAEGGFEPPLGVREITAEVAWMDDEPLVPGRMYWALQGHRWVKAKIKRIVHQVNINTLETHDATHLDANAIGVVE
LALLEPIPARPYLQSRILGSLVLVDTASHKTSGAALVL
>Mature_437_residues
STPTVETVSLTTAATAQDTRAALRFVTCGSVDDGKSTLIGRLLVDSRAVLQDHLAGVQRGGETDLALLTDGLSAEREQGI
TIDVAYRYFSTESRKFIIADAPGHEQYTRNMVTAASSADAAVVLVDATKLTWEAPSPELLPQTRRHSLLVDLLRVPSIVF
AINKLDAVANPALAFAHISAALSQFAKEAQIPVKAIVPVSALIGYNVVDAHPGWCDYQGPSLLEILEHLPATPADTSEAF
AFPVQWVEKFSSSADTSQGRRIFWGRIASGAVQPGQQITVLPGGKSATVAQVLDHTRQPKAVLAGHSAGIILDRELDVSR
GDWLLAEGGFEPPLGVREITAEVAWMDDEPLVPGRMYWALQGHRWVKAKIKRIVHQVNINTLETHDATHLDANAIGVVEL
ALLEPIPARPYLQSRILGSLVLVDTASHKTSGAALVL

Specific function: APS kinase catalyzes the synthesis of activated sulfate [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=425, Percent_Identity=28.2352941176471, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI5729864, Length=425, Percent_Identity=28.2352941176471, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI4503475, Length=443, Percent_Identity=27.313769751693, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI4503471, Length=354, Percent_Identity=29.0960451977401, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI194018522, Length=305, Percent_Identity=28.8524590163934, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI46094014, Length=308, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI194018520, Length=305, Percent_Identity=28.8524590163934, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI194097354, Length=305, Percent_Identity=28.8524590163934, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI34147630, Length=318, Percent_Identity=27.6729559748428, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1789108, Length=432, Percent_Identity=42.3611111111111, Blast_Score=312, Evalue=3e-86,
Organism=Escherichia coli, GI1790412, Length=329, Percent_Identity=26.1398176291793, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789737, Length=329, Percent_Identity=26.1398176291793, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI32566629, Length=436, Percent_Identity=27.5229357798165, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=322, Percent_Identity=31.055900621118, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17552884, Length=433, Percent_Identity=27.7136258660508, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17569207, Length=433, Percent_Identity=27.7136258660508, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532065, Length=322, Percent_Identity=31.055900621118, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32566303, Length=433, Percent_Identity=25.4041570438799, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI32566301, Length=146, Percent_Identity=37.6712328767123, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI25141371, Length=308, Percent_Identity=25.3246753246753, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=324, Percent_Identity=27.7777777777778, Blast_Score=75, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322937, Length=434, Percent_Identity=29.2626728110599, Blast_Score=148, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6325337, Length=346, Percent_Identity=28.9017341040462, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6319594, Length=346, Percent_Identity=28.9017341040462, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320377, Length=423, Percent_Identity=26.241134751773, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6324761, Length=342, Percent_Identity=26.9005847953216, Blast_Score=91, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24652838, Length=414, Percent_Identity=26.8115942028986, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17137572, Length=414, Percent_Identity=26.8115942028986, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI45553807, Length=412, Percent_Identity=26.4563106796117, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45553816, Length=412, Percent_Identity=26.4563106796117, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24651721, Length=412, Percent_Identity=26.4563106796117, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17864154, Length=412, Percent_Identity=26.4563106796117, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17137380, Length=341, Percent_Identity=27.2727272727273, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI45550900, Length=308, Percent_Identity=29.2207792207792, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281363316, Length=259, Percent_Identity=30.1158301158301, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17864358, Length=259, Percent_Identity=30.1158301158301, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19921738, Length=225, Percent_Identity=28.4444444444444, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF01583 APS_kinase; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 47059; Mature: 46928

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPTVETVSLTTAATAQDTRAALRFVTCGSVDDGKSTLIGRLLVDSRAVLQDHLAGVQR
CCCCCEEEEEEEEECCCHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GGETDLALLTDGLSAEREQGITIDVAYRYFSTESRKFIIADAPGHEQYTRNMVTAASSAD
CCCCCEEEEECCCCCHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCC
AAVVLVDATKLTWEAPSPELLPQTRRHSLLVDLLRVPSIVFAINKLDAVANPALAFAHIS
EEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AALSQFAKEAQIPVKAIVPVSALIGYNVVDAHPGWCDYQGPSLLEILEHLPATPADTSEA
HHHHHHHHHHCCCEEEHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
FAFPVQWVEKFSSSADTSQGRRIFWGRIASGAVQPGQQITVLPGGKSATVAQVLDHTRQP
EEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCC
KAVLAGHSAGIILDRELDVSRGDWLLAEGGFEPPLGVREITAEVAWMDDEPLVPGRMYWA
CEEEECCCCCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHEEEECCCCCCCCCEEEEE
LQGHRWVKAKIKRIVHQVNINTLETHDATHLDANAIGVVELALLEPIPARPYLQSRILGS
ECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHEEEEECCCCCCCHHHHHHHHCE
LVLVDTASHKTSGAALVL
EEEEECCCCCCCCCEEEC
>Mature Secondary Structure 
STPTVETVSLTTAATAQDTRAALRFVTCGSVDDGKSTLIGRLLVDSRAVLQDHLAGVQR
CCCCEEEEEEEEECCCHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
GGETDLALLTDGLSAEREQGITIDVAYRYFSTESRKFIIADAPGHEQYTRNMVTAASSAD
CCCCCEEEEECCCCCHHHCCCEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHCCCCCC
AAVVLVDATKLTWEAPSPELLPQTRRHSLLVDLLRVPSIVFAINKLDAVANPALAFAHIS
EEEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
AALSQFAKEAQIPVKAIVPVSALIGYNVVDAHPGWCDYQGPSLLEILEHLPATPADTSEA
HHHHHHHHHHCCCEEEHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCC
FAFPVQWVEKFSSSADTSQGRRIFWGRIASGAVQPGQQITVLPGGKSATVAQVLDHTRQP
EEHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCC
KAVLAGHSAGIILDRELDVSRGDWLLAEGGFEPPLGVREITAEVAWMDDEPLVPGRMYWA
CEEEECCCCCEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHEEEECCCCCCCCCEEEEE
LQGHRWVKAKIKRIVHQVNINTLETHDATHLDANAIGVVELALLEPIPARPYLQSRILGS
ECCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHEEEEECCCCCCCHHHHHHHHCE
LVLVDTASHKTSGAALVL
EEEEECCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]