Click here to switch to the map view.

The map label for this gene is yiaJ [H]

Identifier: 91788193

GI number: 91788193

Start: 2419604

End: 2420383

Strand: Reverse

Name: yiaJ [H]

Synonym: Bpro_2324

Alternate gene names: 91788193

Gene position: 2420383-2419604 (Counterclockwise)

Preceding gene: 91788194

Following gene: 91788185

Centisome position: 46.54

GC content: 58.97

Gene sequence:

>780_bases
ATGAAAAAGTCCGACGCCACCCTCGCCGCAACCCCGACCGTTCAGGTCATCGAACGCATGTTCAATCTGCTGGAAATTCT
CGCCAATCGCGAAGAGGCGGTGTCCCTCAAGGAGATCAGCGAAAAATCCGGACTTCACCCCTCCACCGCCCACCGCATAC
TGAACGACCTGGCCACTGGACGTTTTGTCGACCGCCCCTCGGCGGGCAACTATCGGCTGGGCATGCGCCTCCTGGAGCTC
GGGAATCTCGTCAAGGCGCGATTGAATGTCCGCGACGCAGCCCTTACGCCGATGCGCGAATTGCACAAGCTCACTCAGCA
GCCCGTTAACCTCAGCATGCGGCAAGGTGATGAAATTGTGTATGTGGAGCGTGCTTACAGCGAACGATCCGGGATGCAGG
TGGTGCGGGCCATCGGTGGGCGGGCACCGCTGCATTTAACCTCGGTGGGTAAGCTGTTTCTGGCTTTTGACGATCCCCAA
CGACTGCGCACCTATGCCACTCGCACCGGCCTGTCGGGACAGACCCGAAACAGCATCACGCAGTTATCGGTACTGGAACG
CGAACTCGCCAAAGCGCGGCAATACGGCATCGCGCGCGACAATGAAGAGTTGGAATTGGGTGTGCGTTGCATGGCCGCCG
GCATCTACGACGATCAGAACCAATTGGTGGCAGGTCTTTCCATTTCGGCGCCCGCCGACAGGCTCGATGAGCACTGGCTA
CCCAAACTGCAGGCGACTGCCAACGAAATTTCAGCCGCACTGGGCTACAAACCCAAGTAA

Upstream 100 bases:

>100_bases
GACTGACCTGGCCAAAGGAAATACAACCGGACACCAGCAAACAAAATCAGCAGATGCATTGTGATTTCATAGCGTAAAAT
TTAATGCGTATAGTCTGCAC

Downstream 100 bases:

>100_bases
AAAGGAAGCGAGCCACCTTGGCTCTGCGTCGAGTCGCGATGAAAGTCGCGATGAAAATAGTGCGGGCTACCCGCACGAAA
CATCAACCGCCAATCTGGCT

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKKSDATLAATPTVQVIERMFNLLEILANREEAVSLKEISEKSGLHPSTAHRILNDLATGRFVDRPSAGNYRLGMRLLEL
GNLVKARLNVRDAALTPMRELHKLTQQPVNLSMRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAFDDPQ
RLRTYATRTGLSGQTRNSITQLSVLERELAKARQYGIARDNEELELGVRCMAAGIYDDQNQLVAGLSISAPADRLDEHWL
PKLQATANEISAALGYKPK

Sequences:

>Translated_259_residues
MKKSDATLAATPTVQVIERMFNLLEILANREEAVSLKEISEKSGLHPSTAHRILNDLATGRFVDRPSAGNYRLGMRLLEL
GNLVKARLNVRDAALTPMRELHKLTQQPVNLSMRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAFDDPQ
RLRTYATRTGLSGQTRNSITQLSVLERELAKARQYGIARDNEELELGVRCMAAGIYDDQNQLVAGLSISAPADRLDEHWL
PKLQATANEISAALGYKPK
>Mature_259_residues
MKKSDATLAATPTVQVIERMFNLLEILANREEAVSLKEISEKSGLHPSTAHRILNDLATGRFVDRPSAGNYRLGMRLLEL
GNLVKARLNVRDAALTPMRELHKLTQQPVNLSMRQGDEIVYVERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAFDDPQ
RLRTYATRTGLSGQTRNSITQLSVLERELAKARQYGIARDNEELELGVRCMAAGIYDDQNQLVAGLSISAPADRLDEHWL
PKLQATANEISAALGYKPK

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=250, Percent_Identity=33.6, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1786468, Length=255, Percent_Identity=31.7647058823529, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1788131, Length=259, Percent_Identity=30.5019305019305, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1786715, Length=248, Percent_Identity=31.4516129032258, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI87082359, Length=261, Percent_Identity=29.5019157088123, Blast_Score=107, Evalue=7e-25,
Organism=Escherichia coli, GI1790752, Length=228, Percent_Identity=27.6315789473684, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788581, Length=249, Percent_Identity=27.3092369477912, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 28731; Mature: 28731

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSDATLAATPTVQVIERMFNLLEILANREEAVSLKEISEKSGLHPSTAHRILNDLATG
CCCCCCCEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
RFVDRPSAGNYRLGMRLLELGNLVKARLNVRDAALTPMRELHKLTQQPVNLSMRQGDEIV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHCCCCCCHHCCCCCEE
YVERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAFDDPQRLRTYATRTGLSGQTRNSIT
EEEHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHH
QLSVLERELAKARQYGIARDNEELELGVRCMAAGIYDDQNQLVAGLSISAPADRLDEHWL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECEECCCCHHHHHHHHC
PKLQATANEISAALGYKPK
HHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKSDATLAATPTVQVIERMFNLLEILANREEAVSLKEISEKSGLHPSTAHRILNDLATG
CCCCCCCEEECCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
RFVDRPSAGNYRLGMRLLELGNLVKARLNVRDAALTPMRELHKLTQQPVNLSMRQGDEIV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHCCHHHHHHHHCCCCCCHHCCCCCEE
YVERAYSERSGMQVVRAIGGRAPLHLTSVGKLFLAFDDPQRLRTYATRTGLSGQTRNSIT
EEEHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHH
QLSVLERELAKARQYGIARDNEELELGVRCMAAGIYDDQNQLVAGLSISAPADRLDEHWL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECEECCCCHHHHHHHHC
PKLQATANEISAALGYKPK
HHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]