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The map label for this gene is 91788172

Identifier: 91788172

GI number: 91788172

Start: 2397536

End: 2399257

Strand: Reverse

Name: 91788172

Synonym: Bpro_2303

Alternate gene names: NA

Gene position: 2399257-2397536 (Counterclockwise)

Preceding gene: 91788174

Following gene: 91788171

Centisome position: 46.14

GC content: 61.32

Gene sequence:

>1722_bases
ATGAAATCCCTCACCGGCCTCGACGCGACCTTTCTCTACCTCGAAACGCCGGAAATGCCCATGCATGTGGGCTCACTGAA
CCTGTGCGAGTTACCGGCCGGTTTCAGGGGCAGCTTTCACAAAGAGGTCACCCAACATATTGCCAGGCGCATGCATCTGG
CGCCCGTGTTCAGCCGCAAGCTGGTGTTCATGCCGCTGGACCTCGGTCATCCGTTGTGGGTCGAGGCTGACTCGGTCGAC
ATCGGCTTTCACATCCGCCGCGCCGACAGCGCCAAAAAAGGCGCTGCGCCGATGACGCTGGCCGAGGTGCACAAGCTGTG
CGCGCAACTGCATGGCGAGCTGATTGACCGCAACTTTCCCCTCTGGGAGTTCTATATTTTTGACCGGATCATGCTGCCGG
CCAGCCAGGGCGGTAAGGTTGTGGCTGGCTTTTTTTCCAAAATCCATCATGCTGCGCTGGACGGAAAAGGCGGCGTGCTG
CTGGCCAACGCCCTGCTGGACTTCAGCCCGGTGCCGCGTGAGGTGCCCCCGCCCGACCCGAGCCGTCGCCGCAAGCTGGA
GGCCGACCTCAAGCTCGGCAAGATGATCGGCTCGGTGTTTTCCAGCTCGCTGGGCCAGTTGGTCAAGGTGGCCAGGTCAT
TGCCCGCAGCAGCCAGCACCTTCGGCAGCACGCTGGCCAAACAGTCTGTTGCCAGTTCGGCCACCGGCGTGCGGCCGAAG
ATGCCGCTGAAGCTGGCGCCACCCACACCGTTTAATGCGGGCATCGGTTCGGGCCGGGTGTTCGTCACCGCCACCCTTCC
GCTGGCTGAATGCAAGGCAGTGGGCAAGGCGGTAGGTGGCTCGTTCAATGACGTGGTGCTGTGGATCTGCTCGACCGCCC
TGCGCGATTACCTCACGAAGCACCACAGCCTGCCCAAAAAATCGCTGGTGGCGGTCATGCCGGTCTCGCTGCGCGAAGAT
GGCGCTACCGACGCCAGCAAACTGGGCAACCAGGTGAGCATGAGCCTGGTGGAGTTGGGCACGCACCTGACGCACCCGCT
CAAGCGCATGAATGCCATCATGGCGTCCACCACCAGGGTGAAAGACTCCCTGCTAAGCTTGAAAGGCCTGTTGCCGACCG
ACTACCCGTCGCTGCTGGCGCCGTGGCTGGTGGGCGGCGCGGCCAGGCTGGCGCTGAATGCCTATGGCAAAAGTGGCCTG
GCCAGCCACCTGCCGATGGTGGCAAACCTCGCGATTTCGAATGTGCCAGGATCCGCAGTGCCCCTGTACCTGGCCGGCGC
CCGCTTTCTGACCTTCCACCCGCTGTCCATCATCATGCATGGCCTGGCACTCAACATCACCATCCAGACGTACGCCGGCC
ATGTTGATTTCGGCATTATTGGTGACAAGAAAGCGCTGCCGCATGCGCAAGACCTGGCCAAGGCGATCGAGGATGCTTTC
GGGCAAGCGCAGGCATTGATGGCCGGTACAACATCCGCTACAACTGCAAGCGGGCCAACGAAGGTCAAGACACCCGTAAA
AACCCGCAGAACTGCCGTGGTAAACGTGACCAACGCAACCAAGTTAGAGGGGCCTCCCTCTGGAAAAAGAAGCAGACGCC
CTGATACTGTTTCCATACATAACAACACCAAAACGTCCAATGCTTCGAGCCATGGCAAGCACATCCCGCAAAAAGCCCGC
ACCCCGCGAATCCAGCGCACAAGACCCCCCAGGCCAGCATGA

Upstream 100 bases:

>100_bases
CGTTGCGGGGGCCAGCGCCTTTGTGACAGGCCCGCGTACGGCAAACCCTAGTGCCGCGACTTGCCGCGCTCGGTTAACGT
CCCCCACGGAGACAACCACC

Downstream 100 bases:

>100_bases
CATTGCGCCGCCCAGCGCCTGGCTGCTGGCGCTGGAGCTGCGTGCCTTCTGGGAATTTGGCGCCCTGCTGCCCTCATGGC
CGCTGCTGGCACGCGCGCCC

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv1425/MT1468 [H]

Number of amino acids: Translated: 573; Mature: 573

Protein sequence:

>573_residues
MKSLTGLDATFLYLETPEMPMHVGSLNLCELPAGFRGSFHKEVTQHIARRMHLAPVFSRKLVFMPLDLGHPLWVEADSVD
IGFHIRRADSAKKGAAPMTLAEVHKLCAQLHGELIDRNFPLWEFYIFDRIMLPASQGGKVVAGFFSKIHHAALDGKGGVL
LANALLDFSPVPREVPPPDPSRRRKLEADLKLGKMIGSVFSSSLGQLVKVARSLPAAASTFGSTLAKQSVASSATGVRPK
MPLKLAPPTPFNAGIGSGRVFVTATLPLAECKAVGKAVGGSFNDVVLWICSTALRDYLTKHHSLPKKSLVAVMPVSLRED
GATDASKLGNQVSMSLVELGTHLTHPLKRMNAIMASTTRVKDSLLSLKGLLPTDYPSLLAPWLVGGAARLALNAYGKSGL
ASHLPMVANLAISNVPGSAVPLYLAGARFLTFHPLSIIMHGLALNITIQTYAGHVDFGIIGDKKALPHAQDLAKAIEDAF
GQAQALMAGTTSATTASGPTKVKTPVKTRRTAVVNVTNATKLEGPPSGKRSRRPDTVSIHNNTKTSNASSHGKHIPQKAR
TPRIQRTRPPRPA

Sequences:

>Translated_573_residues
MKSLTGLDATFLYLETPEMPMHVGSLNLCELPAGFRGSFHKEVTQHIARRMHLAPVFSRKLVFMPLDLGHPLWVEADSVD
IGFHIRRADSAKKGAAPMTLAEVHKLCAQLHGELIDRNFPLWEFYIFDRIMLPASQGGKVVAGFFSKIHHAALDGKGGVL
LANALLDFSPVPREVPPPDPSRRRKLEADLKLGKMIGSVFSSSLGQLVKVARSLPAAASTFGSTLAKQSVASSATGVRPK
MPLKLAPPTPFNAGIGSGRVFVTATLPLAECKAVGKAVGGSFNDVVLWICSTALRDYLTKHHSLPKKSLVAVMPVSLRED
GATDASKLGNQVSMSLVELGTHLTHPLKRMNAIMASTTRVKDSLLSLKGLLPTDYPSLLAPWLVGGAARLALNAYGKSGL
ASHLPMVANLAISNVPGSAVPLYLAGARFLTFHPLSIIMHGLALNITIQTYAGHVDFGIIGDKKALPHAQDLAKAIEDAF
GQAQALMAGTTSATTASGPTKVKTPVKTRRTAVVNVTNATKLEGPPSGKRSRRPDTVSIHNNTKTSNASSHGKHIPQKAR
TPRIQRTRPPRPA
>Mature_573_residues
MKSLTGLDATFLYLETPEMPMHVGSLNLCELPAGFRGSFHKEVTQHIARRMHLAPVFSRKLVFMPLDLGHPLWVEADSVD
IGFHIRRADSAKKGAAPMTLAEVHKLCAQLHGELIDRNFPLWEFYIFDRIMLPASQGGKVVAGFFSKIHHAALDGKGGVL
LANALLDFSPVPREVPPPDPSRRRKLEADLKLGKMIGSVFSSSLGQLVKVARSLPAAASTFGSTLAKQSVASSATGVRPK
MPLKLAPPTPFNAGIGSGRVFVTATLPLAECKAVGKAVGGSFNDVVLWICSTALRDYLTKHHSLPKKSLVAVMPVSLRED
GATDASKLGNQVSMSLVELGTHLTHPLKRMNAIMASTTRVKDSLLSLKGLLPTDYPSLLAPWLVGGAARLALNAYGKSGL
ASHLPMVANLAISNVPGSAVPLYLAGARFLTFHPLSIIMHGLALNITIQTYAGHVDFGIIGDKKALPHAQDLAKAIEDAF
GQAQALMAGTTSATTASGPTKVKTPVKTRRTAVVNVTNATKLEGPPSGKRSRRPDTVSIHNNTKTSNASSHGKHIPQKAR
TPRIQRTRPPRPA

Specific function: Upon expression in E.coli functions very weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has no wax synthase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 61201; Mature: 61201

Theoretical pI: Translated: 10.84; Mature: 10.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLTGLDATFLYLETPEMPMHVGSLNLCELPAGFRGSFHKEVTQHIARRMHLAPVFSRK
CCCCCCCCEEEEEEECCCCCEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LVFMPLDLGHPLWVEADSVDIGFHIRRADSAKKGAAPMTLAEVHKLCAQLHGELIDRNFP
EEEEEECCCCCEEEECCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
LWEFYIFDRIMLPASQGGKVVAGFFSKIHHAALDGKGGVLLANALLDFSPVPREVPPPDP
EEEHEEHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCCC
SRRRKLEADLKLGKMIGSVFSSSLGQLVKVARSLPAAASTFGSTLAKQSVASSATGVRPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
MPLKLAPPTPFNAGIGSGRVFVTATLPLAECKAVGKAVGGSFNDVVLWICSTALRDYLTK
CCEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HHSLPKKSLVAVMPVSLREDGATDASKLGNQVSMSLVELGTHLTHPLKRMNAIMASTTRV
HHCCCHHHEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDSLLSLKGLLPTDYPSLLAPWLVGGAARLALNAYGKSGLASHLPMVANLAISNVPGSAV
HHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCHHHHHHHCCCCCCCC
PLYLAGARFLTFHPLSIIMHGLALNITIQTYAGHVDFGIIGDKKALPHAQDLAKAIEDAF
EEEEECCHHEEECHHHHHHCCEEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHH
GQAQALMAGTTSATTASGPTKVKTPVKTRRTAVVNVTNATKLEGPPSGKRSRRPDTVSIH
HHHHHHHCCCCCCCCCCCCCEECCCCHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
NNTKTSNASSHGKHIPQKARTPRIQRTRPPRPA
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSLTGLDATFLYLETPEMPMHVGSLNLCELPAGFRGSFHKEVTQHIARRMHLAPVFSRK
CCCCCCCCEEEEEEECCCCCEECCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LVFMPLDLGHPLWVEADSVDIGFHIRRADSAKKGAAPMTLAEVHKLCAQLHGELIDRNFP
EEEEEECCCCCEEEECCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
LWEFYIFDRIMLPASQGGKVVAGFFSKIHHAALDGKGGVLLANALLDFSPVPREVPPPDP
EEEHEEHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCCCCCCCCC
SRRRKLEADLKLGKMIGSVFSSSLGQLVKVARSLPAAASTFGSTLAKQSVASSATGVRPK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCC
MPLKLAPPTPFNAGIGSGRVFVTATLPLAECKAVGKAVGGSFNDVVLWICSTALRDYLTK
CCEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
HHSLPKKSLVAVMPVSLREDGATDASKLGNQVSMSLVELGTHLTHPLKRMNAIMASTTRV
HHCCCHHHEEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDSLLSLKGLLPTDYPSLLAPWLVGGAARLALNAYGKSGLASHLPMVANLAISNVPGSAV
HHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCHHHHCCHHHHHHHCCCCCCCC
PLYLAGARFLTFHPLSIIMHGLALNITIQTYAGHVDFGIIGDKKALPHAQDLAKAIEDAF
EEEEECCHHEEECHHHHHHCCEEEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHH
GQAQALMAGTTSATTASGPTKVKTPVKTRRTAVVNVTNATKLEGPPSGKRSRRPDTVSIH
HHHHHHHCCCCCCCCCCCCCEECCCCHHCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEE
NNTKTSNASSHGKHIPQKARTPRIQRTRPPRPA
CCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]