Click here to switch to the map view.

The map label for this gene is phbC [H]

Identifier: 91788012

GI number: 91788012

Start: 2229453

End: 2231195

Strand: Direct

Name: phbC [H]

Synonym: Bpro_2139

Alternate gene names: 91788012

Gene position: 2229453-2231195 (Clockwise)

Preceding gene: 91788010

Following gene: 91788013

Centisome position: 42.87

GC content: 59.15

Gene sequence:

>1743_bases
ATGAATTCTGACGCACAGTGGTCCCAGGCGGCCCAACAGATGCAGGAAACCTTTGGCGCGGGCTTCCAGCAGATGCTGGG
CGCATTCGGAACAGGCAGCCCTCCGGCCGGTTTGCCGCAAATCGATCTGTCCACGTTCCAGCAGGCCCTGCCGTCCCTCC
GCTTCGATCCCGTCAAGCTGCAAGAACTGCAGCAGCAATACATTCAAAACGCCTCAGTGCTCTGGAACCAGGGGGTTCAG
GCTGGTGTCGACGCAGCCACCGCATCCGACCGGCGTTTTGCCGCCGAAGCCTGGAATGCCAATCCTGTCTCCAGGTTCTC
GGCCTCCACCTACCTGCTCAATGCCCGTACCCTGATGGGGCTGGCCGAAGCGGTGGAGGGCGATGCGAAAACCAAGGCAC
GTCTGCGATTTGCGGTTGAGCAGTGGATGGCGGCAACGGCGCCCAGCAATTTTCTGGCGCTGAATGCCGAAGCCCAGCAA
AAGGCCATCGCCAGCAAGGGCGAGAGCATTGCGCGTGGCATCCAGAACCTGCTGCACGACATGAGACAGGGCCATCTGTC
GATGACCGACGAAAGTGTGTTCGAGGTGGGCAAGAATGTTGCCACCACGGAAGGCGCGGTGGTCTTTGAAAACGAATTCT
TCCAGCTGCTGGAATACAAGCCGCTGACGGCGAGGGTCTATGAAAAACCCTTCCTGCTCGTTCCGCCCTGCATCAACAAG
TTTTATATTCTGGATCTGCAACCCGACAATTCGCTGATCCGCTACGCGGTCAGCCAGGGCCAACGTACCTTCGTGGTGAG
CTGGCGCAACCCTGACGAGTCCATGACCGGCAACACATGGGATGACTATATCGAGCACGGTGCCATCAAGGCCATCGAGG
TGGTGCAGGAAATCACCGGTGCCAAAACCATCAATGCGCTGGGTTTTTGCGTTGGCGGCACTATTTTGGGAACGGCGCTT
GCCGTGCTGGCTGCACGCGGCGAGAAGCCGGTGACCAGCCTCACGCTGCTGACTTCACTGCTTGACTTCACCGACACCGG
CATCCTGGATATTTTCATTGACGAGAATTCCGTCAAATACCGTGAGGCGGAGATGGGGAAAGGGGGCTTGCTCAAGGGGC
AGGACCTGGCGGCTACCTTCAGCTTTCTGCGACCCAACGATCTGGTCTGGAATTACGTCGTGGGCAATTACCTGAAAGGC
GAAACACCGCCGCCGTTCGACCTGCTCTACTGGAACAGCGACTCCACCAACCTGCCGGGCCCGTTTTACGCCTGGTACCT
GCGCAACACCTATTTTGAAAACAACCTGGTCAAACCTGGCAAAACCGTGGTGTGCGGCCAAAAGGTTGATCTGGGCAAGC
TCGACATGCCCGTTTATATCTACGGCTCGCGCGAGGACCATATCGTGCCCATTGGCGGAGCCTATGCGTCAACGCAGCAG
CTGCCGGGCAAAAAGCGTTTTGTGATGGGCGCTTCCGGCCATATTGCCGGCGTGATCAATCCGCCGGCCAAGAAAAAACG
CAGCTACTGGACCAACGATAAGCTGCCGCAGGGCAAGTTTCCCGCGGAGCAGGCTGAGTGGCAGAAAAATGCCACAGAAC
ACCCCGGCAGCTGGTGGACGGACTGGTCCGACTGGCTCGAGGGCCATGCCGGCAAGCAAATTGCCGCGCCCAAGGGCTAC
GGCGGTCGCCAGCACAAGAAGATCGAGCCCGCGCCGGGCCGGTACGTCAAGGTGAAGGCCTGA

Upstream 100 bases:

>100_bases
AGTTCGGTCAGCAATTTAGAAGAAAACCCTACGATACTGAAAGCCTGTTTAAAAAGGCGAAAAAAGGGTCTTCAGGACTC
CATACCGAGAAGGAGGCAAG

Downstream 100 bases:

>100_bases
AGATGAGCGTCTCAATCTTCACCTCAAGCCTCCATAAAAGCGTGAAAAGCATCCAATAATTTACAGGGCTCAGGTATCGA
AAAGCCCATGGCAGACGCTC

Product: poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 580; Mature: 580

Protein sequence:

>580_residues
MNSDAQWSQAAQQMQETFGAGFQQMLGAFGTGSPPAGLPQIDLSTFQQALPSLRFDPVKLQELQQQYIQNASVLWNQGVQ
AGVDAATASDRRFAAEAWNANPVSRFSASTYLLNARTLMGLAEAVEGDAKTKARLRFAVEQWMAATAPSNFLALNAEAQQ
KAIASKGESIARGIQNLLHDMRQGHLSMTDESVFEVGKNVATTEGAVVFENEFFQLLEYKPLTARVYEKPFLLVPPCINK
FYILDLQPDNSLIRYAVSQGQRTFVVSWRNPDESMTGNTWDDYIEHGAIKAIEVVQEITGAKTINALGFCVGGTILGTAL
AVLAARGEKPVTSLTLLTSLLDFTDTGILDIFIDENSVKYREAEMGKGGLLKGQDLAATFSFLRPNDLVWNYVVGNYLKG
ETPPPFDLLYWNSDSTNLPGPFYAWYLRNTYFENNLVKPGKTVVCGQKVDLGKLDMPVYIYGSREDHIVPIGGAYASTQQ
LPGKKRFVMGASGHIAGVINPPAKKKRSYWTNDKLPQGKFPAEQAEWQKNATEHPGSWWTDWSDWLEGHAGKQIAAPKGY
GGRQHKKIEPAPGRYVKVKA

Sequences:

>Translated_580_residues
MNSDAQWSQAAQQMQETFGAGFQQMLGAFGTGSPPAGLPQIDLSTFQQALPSLRFDPVKLQELQQQYIQNASVLWNQGVQ
AGVDAATASDRRFAAEAWNANPVSRFSASTYLLNARTLMGLAEAVEGDAKTKARLRFAVEQWMAATAPSNFLALNAEAQQ
KAIASKGESIARGIQNLLHDMRQGHLSMTDESVFEVGKNVATTEGAVVFENEFFQLLEYKPLTARVYEKPFLLVPPCINK
FYILDLQPDNSLIRYAVSQGQRTFVVSWRNPDESMTGNTWDDYIEHGAIKAIEVVQEITGAKTINALGFCVGGTILGTAL
AVLAARGEKPVTSLTLLTSLLDFTDTGILDIFIDENSVKYREAEMGKGGLLKGQDLAATFSFLRPNDLVWNYVVGNYLKG
ETPPPFDLLYWNSDSTNLPGPFYAWYLRNTYFENNLVKPGKTVVCGQKVDLGKLDMPVYIYGSREDHIVPIGGAYASTQQ
LPGKKRFVMGASGHIAGVINPPAKKKRSYWTNDKLPQGKFPAEQAEWQKNATEHPGSWWTDWSDWLEGHAGKQIAAPKGY
GGRQHKKIEPAPGRYVKVKA
>Mature_580_residues
MNSDAQWSQAAQQMQETFGAGFQQMLGAFGTGSPPAGLPQIDLSTFQQALPSLRFDPVKLQELQQQYIQNASVLWNQGVQ
AGVDAATASDRRFAAEAWNANPVSRFSASTYLLNARTLMGLAEAVEGDAKTKARLRFAVEQWMAATAPSNFLALNAEAQQ
KAIASKGESIARGIQNLLHDMRQGHLSMTDESVFEVGKNVATTEGAVVFENEFFQLLEYKPLTARVYEKPFLLVPPCINK
FYILDLQPDNSLIRYAVSQGQRTFVVSWRNPDESMTGNTWDDYIEHGAIKAIEVVQEITGAKTINALGFCVGGTILGTAL
AVLAARGEKPVTSLTLLTSLLDFTDTGILDIFIDENSVKYREAEMGKGGLLKGQDLAATFSFLRPNDLVWNYVVGNYLKG
ETPPPFDLLYWNSDSTNLPGPFYAWYLRNTYFENNLVKPGKTVVCGQKVDLGKLDMPVYIYGSREDHIVPIGGAYASTQQ
LPGKKRFVMGASGHIAGVINPPAKKKRSYWTNDKLPQGKFPAEQAEWQKNATEHPGSWWTDWSDWLEGHAGKQIAAPKGY
GGRQHKKIEPAPGRYVKVKA

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 63964; Mature: 63964

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDAQWSQAAQQMQETFGAGFQQMLGAFGTGSPPAGLPQIDLSTFQQALPSLRFDPVKL
CCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHH
QELQQQYIQNASVLWNQGVQAGVDAATASDRRFAAEAWNANPVSRFSASTYLLNARTLMG
HHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHCCCCCCHHHHCCCEEEEHHHHHHH
LAEAVEGDAKTKARLRFAVEQWMAATAPSNFLALNAEAQQKAIASKGESIARGIQNLLHD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHH
MRQGHLSMTDESVFEVGKNVATTEGAVVFENEFFQLLEYKPLTARVYEKPFLLVPPCINK
HHCCCCCCCHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHCCCCEEECCCCCC
FYILDLQPDNSLIRYAVSQGQRTFVVSWRNPDESMTGNTWDDYIEHGAIKAIEVVQEITG
EEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHC
AKTINALGFCVGGTILGTALAVLAARGEKPVTSLTLLTSLLDFTDTGILDIFIDENSVKY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
REAEMGKGGLLKGQDLAATFSFLRPNDLVWNYVVGNYLKGETPPPFDLLYWNSDSTNLPG
EHHHCCCCCCCCCCHHHHHHHHCCCCCCEEHHHHCCCCCCCCCCCCEEEEECCCCCCCCC
PFYAWYLRNTYFENNLVKPGKTVVCGQKVDLGKLDMPVYIYGSREDHIVPIGGAYASTQQ
CEEEEEHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCEEECCCCCHHHHH
LPGKKRFVMGASGHIAGVINPPAKKKRSYWTNDKLPQGKFPAEQAEWQKNATEHPGSWWT
CCCCCEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
DWSDWLEGHAGKQIAAPKGYGGRQHKKIEPAPGRYVKVKA
CHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEC
>Mature Secondary Structure
MNSDAQWSQAAQQMQETFGAGFQQMLGAFGTGSPPAGLPQIDLSTFQQALPSLRFDPVKL
CCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHH
QELQQQYIQNASVLWNQGVQAGVDAATASDRRFAAEAWNANPVSRFSASTYLLNARTLMG
HHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHCCCCCCHHHHCCCEEEEHHHHHHH
LAEAVEGDAKTKARLRFAVEQWMAATAPSNFLALNAEAQQKAIASKGESIARGIQNLLHD
HHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHHHH
MRQGHLSMTDESVFEVGKNVATTEGAVVFENEFFQLLEYKPLTARVYEKPFLLVPPCINK
HHCCCCCCCHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCHHHHHCCCCEEECCCCCC
FYILDLQPDNSLIRYAVSQGQRTFVVSWRNPDESMTGNTWDDYIEHGAIKAIEVVQEITG
EEEEEECCCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHHHHHHHC
AKTINALGFCVGGTILGTALAVLAARGEKPVTSLTLLTSLLDFTDTGILDIFIDENSVKY
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCEE
REAEMGKGGLLKGQDLAATFSFLRPNDLVWNYVVGNYLKGETPPPFDLLYWNSDSTNLPG
EHHHCCCCCCCCCCHHHHHHHHCCCCCCEEHHHHCCCCCCCCCCCCEEEEECCCCCCCCC
PFYAWYLRNTYFENNLVKPGKTVVCGQKVDLGKLDMPVYIYGSREDHIVPIGGAYASTQQ
CEEEEEHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCEEECCCCCHHHHH
LPGKKRFVMGASGHIAGVINPPAKKKRSYWTNDKLPQGKFPAEQAEWQKNATEHPGSWWT
CCCCCEEEEECCCCEEEEECCCHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
DWSDWLEGHAGKQIAAPKGYGGRQHKKIEPAPGRYVKVKA
CHHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2670936; 1987116 [H]