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The map label for this gene is glnA [H]

Identifier: 91788005

GI number: 91788005

Start: 2222463

End: 2223959

Strand: Reverse

Name: glnA [H]

Synonym: Bpro_2132

Alternate gene names: 91788005

Gene position: 2223959-2222463 (Counterclockwise)

Preceding gene: 91788006

Following gene: 91788004

Centisome position: 42.77

GC content: 67.67

Gene sequence:

>1497_bases
GTGAGGGCCGTGCCCACCTTCGCGCAGCTCTGCGGAATCCGCGATGCCGCACGCCAGCAAGCGCTGGAACACACCTCCGG
GCTGATCACTGCCAGCGACCTGGAGCTGGTGCGTTTTGCGTGGTGCGACCTGCACGGTGTCACACGCGGTAAAACCCTGG
TGGCCGGCGCCGCCGCAAAGGCCATGCAGGAAGGCGTGGGCCTGGTCAGCACCCTGGTCCTCAAGGACACCTCGGACCGC
AGCACGGCATTCAAAGTATTCGAGCGTGGCGGCCCCGCCGGGTTGCCGGGCTTCGAGTTCGCCAGCAACCTGCTGCTGCT
GGCCGACCCGTCAAGCTTTCGGCAACTGCCCTGGACGCCCGCCACCGGCTGGGTTCAGGGCCAGCTCTGGTTTCAAAACG
GCCAGCCGGTGGAGCTGGACACGCGCCGCGTGCTGCAGCAGGCGCTCGCCGGCCTGGCGCGGGCCGGCTACGGCATGAAG
TGCGGGCTCGAAGTCGAATTCCACATTTACAAGATCACCGAGGAAGGCACCCGGGACCAGCTGGACCCCAACCAGGCCGG
CTGGCCCGGTTTGCCGCCGCAGGTCAGCCTGGTTCACCCGGGCTACAACATGCTGACCGAGGGCTGGTACGACCTGGCCG
AAGAGCCCTTGCGCATCGTGCAGCACACGGCGCAGGCGCTGGGCCTGCCCTTGCTGTCGCTCGAGATCGAGTTGGGCCCC
AGCCAGGTGGAAGCAGTGTTCGAAGCCACCGACGCGCTGACAGCTGCCGACAACATGGTGCTGTTTCGCAATGCGGTCAA
GCAGGCGCTGCGCCGGGCGGGCTACCACGCCAGCTTCATGTGCCGCCCGCCGTTTGCCAACATCATGTCCAGCGGCTGGC
ACCTGCACCAGTCGCTGGTCGACAGTGAAACCGGCCGCAATGTGTTTCAGCGCGAAGCGCCGGCCCCGGGCAGCACGGCA
GCCGATGCCCGGCACACGCTGTCGGAGACGGGCGAGCACTACCTGGCCGGCCTGCTGGCGCATGCGCGTGGCATGGCCGT
GTTCTGCACGCCCACCATCAACGGCTTTGGCCGCTACCGCCCGAATGCGCTGGCGCCGCAGGCTGTGCTCTGGGGCCGCG
ACAACCGCGGCGCCATGCTGCGTGTGGTGGGCCAGTGCGGTGACGGCGCCACGCGCATTGAAAACCGCATCGGCGAGCCC
GCCGCCAACCCCTACCTGTACCTGGCCTCGCAGATTCATGCCGGGCTCGACGGCATCACGCGCCGGCTGAAGGCGCCGCC
CGCCACCGATGCACCCTATGGGGCTAGCGGCACCCTGCTGCCCACCAGCCTGGGCGAAGCGCTGGAGGCCCTGCAAACGG
ACCCGATTCTGGTCAAGGCCTTTGGCAGCGCCTTTGTCGACTACCTCGTGCGCGTCAAGCGGTCCGAGCTGGACCGCTAT
GCGCAAGCTGAAGACAAGGACGACTGGCAGCGCCGTGAATATTTCAGCCGGATTTGA

Upstream 100 bases:

>100_bases
GTGCGCCTTGGACGGGCAGCCCTCACCCCAACCCTCTCCCAGAGGGAGAGGGAGCCAAGATTCCTGCTTTCATTCCCTCT
CCCTCTGGGAGCAGGCTGGG

Downstream 100 bases:

>100_bases
ACGCCTTCTTCAACACTTCATGATTACCATTAACTTCATAGCTTCCGACGGACAACTGACAGGGGCTACAGCCCAAACGG
GCAAGAGCCTGATGCAGGCC

Product: L-glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MRAVPTFAQLCGIRDAARQQALEHTSGLITASDLELVRFAWCDLHGVTRGKTLVAGAAAKAMQEGVGLVSTLVLKDTSDR
STAFKVFERGGPAGLPGFEFASNLLLLADPSSFRQLPWTPATGWVQGQLWFQNGQPVELDTRRVLQQALAGLARAGYGMK
CGLEVEFHIYKITEEGTRDQLDPNQAGWPGLPPQVSLVHPGYNMLTEGWYDLAEEPLRIVQHTAQALGLPLLSLEIELGP
SQVEAVFEATDALTAADNMVLFRNAVKQALRRAGYHASFMCRPPFANIMSSGWHLHQSLVDSETGRNVFQREAPAPGSTA
ADARHTLSETGEHYLAGLLAHARGMAVFCTPTINGFGRYRPNALAPQAVLWGRDNRGAMLRVVGQCGDGATRIENRIGEP
AANPYLYLASQIHAGLDGITRRLKAPPATDAPYGASGTLLPTSLGEALEALQTDPILVKAFGSAFVDYLVRVKRSELDRY
AQAEDKDDWQRREYFSRI

Sequences:

>Translated_498_residues
MRAVPTFAQLCGIRDAARQQALEHTSGLITASDLELVRFAWCDLHGVTRGKTLVAGAAAKAMQEGVGLVSTLVLKDTSDR
STAFKVFERGGPAGLPGFEFASNLLLLADPSSFRQLPWTPATGWVQGQLWFQNGQPVELDTRRVLQQALAGLARAGYGMK
CGLEVEFHIYKITEEGTRDQLDPNQAGWPGLPPQVSLVHPGYNMLTEGWYDLAEEPLRIVQHTAQALGLPLLSLEIELGP
SQVEAVFEATDALTAADNMVLFRNAVKQALRRAGYHASFMCRPPFANIMSSGWHLHQSLVDSETGRNVFQREAPAPGSTA
ADARHTLSETGEHYLAGLLAHARGMAVFCTPTINGFGRYRPNALAPQAVLWGRDNRGAMLRVVGQCGDGATRIENRIGEP
AANPYLYLASQIHAGLDGITRRLKAPPATDAPYGASGTLLPTSLGEALEALQTDPILVKAFGSAFVDYLVRVKRSELDRY
AQAEDKDDWQRREYFSRI
>Mature_498_residues
MRAVPTFAQLCGIRDAARQQALEHTSGLITASDLELVRFAWCDLHGVTRGKTLVAGAAAKAMQEGVGLVSTLVLKDTSDR
STAFKVFERGGPAGLPGFEFASNLLLLADPSSFRQLPWTPATGWVQGQLWFQNGQPVELDTRRVLQQALAGLARAGYGMK
CGLEVEFHIYKITEEGTRDQLDPNQAGWPGLPPQVSLVHPGYNMLTEGWYDLAEEPLRIVQHTAQALGLPLLSLEIELGP
SQVEAVFEATDALTAADNMVLFRNAVKQALRRAGYHASFMCRPPFANIMSSGWHLHQSLVDSETGRNVFQREAPAPGSTA
ADARHTLSETGEHYLAGLLAHARGMAVFCTPTINGFGRYRPNALAPQAVLWGRDNRGAMLRVVGQCGDGATRIENRIGEP
AANPYLYLASQIHAGLDGITRRLKAPPATDAPYGASGTLLPTSLGEALEALQTDPILVKAFGSAFVDYLVRVKRSELDRY
AQAEDKDDWQRREYFSRI

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=483, Percent_Identity=27.536231884058, Blast_Score=163, Evalue=4e-40,
Organism=Escherichia coli, GI1790301, Length=234, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI87081870, Length=363, Percent_Identity=23.4159779614325, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 54245; Mature: 54245

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVPTFAQLCGIRDAARQQALEHTSGLITASDLELVRFAWCDLHGVTRGKTLVAGAAAK
CCCCCCHHHHHCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHH
AMQEGVGLVSTLVLKDTSDRSTAFKVFERGGPAGLPGFEFASNLLLLADPSSFRQLPWTP
HHHHCHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCCHHHHCCEEEEECCHHHCCCCCCC
ATGWVQGQLWFQNGQPVELDTRRVLQQALAGLARAGYGMKCGLEVEFHIYKITEEGTRDQ
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECEEEEEEEEEEECCCCCCC
LDPNQAGWPGLPPQVSLVHPGYNMLTEGWYDLAEEPLRIVQHTAQALGLPLLSLEIELGP
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCH
SQVEAVFEATDALTAADNMVLFRNAVKQALRRAGYHASFMCRPPFANIMSSGWHLHQSLV
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCHHHHHHHH
DSETGRNVFQREAPAPGSTAADARHTLSETGEHYLAGLLAHARGMAVFCTPTINGFGRYR
HHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
PNALAPQAVLWGRDNRGAMLRVVGQCGDGATRIENRIGEPAANPYLYLASQIHAGLDGIT
CCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCEEEHHHHHCCHHHHH
RRLKAPPATDAPYGASGTLLPTSLGEALEALQTDPILVKAFGSAFVDYLVRVKRSELDRY
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQAEDKDDWQRREYFSRI
HCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRAVPTFAQLCGIRDAARQQALEHTSGLITASDLELVRFAWCDLHGVTRGKTLVAGAAAK
CCCCCCHHHHHCCHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHCCCCCCCCHHHHHHHH
AMQEGVGLVSTLVLKDTSDRSTAFKVFERGGPAGLPGFEFASNLLLLADPSSFRQLPWTP
HHHHCHHHHHHHHEECCCCCHHHHHHHHCCCCCCCCCHHHHCCEEEEECCHHHCCCCCCC
ATGWVQGQLWFQNGQPVELDTRRVLQQALAGLARAGYGMKCGLEVEFHIYKITEEGTRDQ
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCEECEEEEEEEEEEECCCCCCC
LDPNQAGWPGLPPQVSLVHPGYNMLTEGWYDLAEEPLRIVQHTAQALGLPLLSLEIELGP
CCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCH
SQVEAVFEATDALTAADNMVLFRNAVKQALRRAGYHASFMCRPPFANIMSSGWHLHQSLV
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHCCHHHHHHHH
DSETGRNVFQREAPAPGSTAADARHTLSETGEHYLAGLLAHARGMAVFCTPTINGFGRYR
HHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCC
PNALAPQAVLWGRDNRGAMLRVVGQCGDGATRIENRIGEPAANPYLYLASQIHAGLDGIT
CCCCCCCCEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCEEEHHHHHCCHHHHH
RRLKAPPATDAPYGASGTLLPTSLGEALEALQTDPILVKAFGSAFVDYLVRVKRSELDRY
HHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AQAEDKDDWQRREYFSRI
HCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7916055 [H]