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The map label for this gene is rutF [H]

Identifier: 91787935

GI number: 91787935

Start: 2139888

End: 2140400

Strand: Direct

Name: rutF [H]

Synonym: Bpro_2059

Alternate gene names: 91787935

Gene position: 2139888-2140400 (Clockwise)

Preceding gene: 91787929

Following gene: 91787936

Centisome position: 41.15

GC content: 58.09

Gene sequence:

>513_bases
ATGAAAACGCCAACTGTCGTAGCAGAGGTTCAGACCGCCTTTCGTGATGCCATGCGCGGCATCGCGTCGACTGTATCAAT
TGTCACCGCAGGAGACCGGAGCCGGGGCCATGGCATGACCGCCACCGCCATCACGTCCGTGTCGCTTGATCCGCCGGCAC
TGCTGGTCTGTATCAATCGCAAAACGCTGCTGCACGACATCATTTCGTCCACCGGCCGGTTTTGCGTCAATGTGCTCGCG
CAGCAACACCGGGAAATATCGAACGCCTTCAGCGGCGGAGTCGCTCCGCATGAGCGATTTTCAATGGGTGACTGGTCCGA
GACCGAAGACGGGCTCAAGTACCTCACCTCAGCCCAGGCGCGCATTTTCTGCGTCAAAAAACTCGCCATCCCTTTTGGTA
CGCACACCATTTTCGTGGGCGAAGTCGGCGATGTGCAGATTGCCGAAAACAGCGCGCCACTCCTCTATCAGAACTCGACT
TACTGCAGCTCCACGCCAGTTGCTGCCCAGTAG

Upstream 100 bases:

>100_bases
CCTACAAGAATTCAATATACAACAGTTGCGTTTGCAATTGTTGCAAGTAAAAATATAGCCAAGCAAGCGCACTCAACCCC
AAAGATCGGAGAGCAACACC

Downstream 100 bases:

>100_bases
ATCGGGGAGCGCATCGACAACGACCTAACCGGGAATTTCACATGAATGATTTTGTTGACCATGCCGTTCTGGCATCACCG
CGTAAAAGCGAAACTGAAAG

Product: flavin reductase-like protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MKTPTVVAEVQTAFRDAMRGIASTVSIVTAGDRSRGHGMTATAITSVSLDPPALLVCINRKTLLHDIISSTGRFCVNVLA
QQHREISNAFSGGVAPHERFSMGDWSETEDGLKYLTSAQARIFCVKKLAIPFGTHTIFVGEVGDVQIAENSAPLLYQNST
YCSSTPVAAQ

Sequences:

>Translated_170_residues
MKTPTVVAEVQTAFRDAMRGIASTVSIVTAGDRSRGHGMTATAITSVSLDPPALLVCINRKTLLHDIISSTGRFCVNVLA
QQHREISNAFSGGVAPHERFSMGDWSETEDGLKYLTSAQARIFCVKKLAIPFGTHTIFVGEVGDVQIAENSAPLLYQNST
YCSSTPVAAQ
>Mature_170_residues
MKTPTVVAEVQTAFRDAMRGIASTVSIVTAGDRSRGHGMTATAITSVSLDPPALLVCINRKTLLHDIISSTGRFCVNVLA
QQHREISNAFSGGVAPHERFSMGDWSETEDGLKYLTSAQARIFCVKKLAIPFGTHTIFVGEVGDVQIAENSAPLLYQNST
YCSSTPVAAQ

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=151, Percent_Identity=29.1390728476821, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 18202; Mature: 18202

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPTVVAEVQTAFRDAMRGIASTVSIVTAGDRSRGHGMTATAITSVSLDPPALLVCINR
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEHEEEEECCCCCEEEEEECC
KTLLHDIISSTGRFCVNVLAQQHREISNAFSGGVAPHERFSMGDWSETEDGLKYLTSAQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHH
RIFCVKKLAIPFGTHTIFVGEVGDVQIAENSAPLLYQNSTYCSSTPVAAQ
HEEEHHHHHCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MKTPTVVAEVQTAFRDAMRGIASTVSIVTAGDRSRGHGMTATAITSVSLDPPALLVCINR
CCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEHEEEEECCCCCEEEEEECC
KTLLHDIISSTGRFCVNVLAQQHREISNAFSGGVAPHERFSMGDWSETEDGLKYLTSAQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCHH
RIFCVKKLAIPFGTHTIFVGEVGDVQIAENSAPLLYQNSTYCSSTPVAAQ
HEEEHHHHHCCCCCEEEEEECCCCEEEECCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA