Click here to switch to the map view.

The map label for this gene is metC [H]

Identifier: 91787915

GI number: 91787915

Start: 2117729

End: 2118967

Strand: Reverse

Name: metC [H]

Synonym: Bpro_2039

Alternate gene names: 91787915

Gene position: 2118967-2117729 (Counterclockwise)

Preceding gene: 91787916

Following gene: 91787914

Centisome position: 40.75

GC content: 64.73

Gene sequence:

>1239_bases
ATGCACCAAGGTCACCCAGGCGCTCCCCGCATGAATAAAACATCGCCGCCGGCTACCTCACCATCCGGTGCGTCGCCCCT
GACCCGTCTGGTCCACGCCGGCAAGCAGCCCGCCTTCGACGGCGGCCGGCCGGTCAACCCGCCCGTGGTGCGCGCCAGCA
CCGTGCTGTTTGACAACACGGCGCAGCAGCGCGAGATGCGCATGCGGCGCGGCGATGAGCGGCTGTTCACCTACGGAGCC
CGTGGCAACCCGACCAATTTCGCACTCGAAGATGTGGTCAGTGAACTCGAAGGCGCCTACCGGACGCGGCTCTTTCCCAC
CGGCCTGGCCGCCATCGCCATGACGCTGCTCTCTTACCTGAAGCCCGGCGACCACCTGCTGATGTCAGACAGCGTGTACG
AGCCGGCGCGCAAGCTGGTGGGCTCCTTCCTCACGCCGTACAACATCCGCTGCACTTTCTTTGCGGCCGATGGCAGCGAC
GTGGAAGAAAAAATCGAAGCCGGCACGTGCATGATTTATGCCGAATGCCCGGGCTCGCTGGTCTACGAAATGTGCGACCT
GCCGCGTCTGGCCGAAATCGCGCGCGTGAGAGGGCTCCTGCTGGTGGCCGACAACACCTGGGGCTCCGGCTGCCAATACC
GGCCGCTGGCTTTGGGCGCCGATGTATCCATCATGGCTGCCACCAAATACCTTTCGGGCCATTCCGACGTGATGATGGGC
ACCGTTGCCACCACGAAAGATACCTGGCAGGCACTGGCCGAACGCAGTGACGCCTTCGGCATGACGGTCAGCCCCGACGA
TGCCTACCTGGTGCTGCGCGGCACACGCAGCCTGGCCGCGCGCCTGGCCCTGCACGAGAGTCATGGCTTGCAAGTGGCGT
ACTGGCTGCAATCACGCAGCGAAGTAGCCACGGTCTTCTGCCCCGCCTTGCCCGATCACCCGGGTCATGCCCTGTGGCAA
AGAGACTGCAAGGGAAGCAATGGCCTGGTTTCTTTCGAACTCCAACCCGGCTTCGACCAGAGAGCCGCCGAGCGGCTGAT
CGACAGCCTTCAATTGTTTGGCATTGGTGCCTCGTGGGGCGGCTATGAAAGCCTGGTCACGCTGGCCGACATGCGCCGCG
CCCGCAGCGTGACCGACTGGTCGGACCGTGGCCAGGTGATCCGGCTGCACATCGGGCTGGAAGACCCGGCCGACCTGCTG
CGCGATTTGCTGCAGGGATTTGCCGCACTCACCGCCTGA

Upstream 100 bases:

>100_bases
CGGCAACACGCCGGCGGCCTTTGCTGCGCAGCTCAAGGAAGACATCGCCCGCTGGTCGCGCGTCGTCAAGCTGTCGGGTG
CGAAACTGGACTGATGTGTC

Downstream 100 bases:

>100_bases
GCCAAACGCCATCTCACGCCGCACGCCGGCGTTCCCTTTTGCGGTCCTTTTTATCTTCTTTCCCCCGAGGTCTTGCACAA
CCATGCTCAGCTATGCGCGC

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase; Osteotoxin [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MHQGHPGAPRMNKTSPPATSPSGASPLTRLVHAGKQPAFDGGRPVNPPVVRASTVLFDNTAQQREMRMRRGDERLFTYGA
RGNPTNFALEDVVSELEGAYRTRLFPTGLAAIAMTLLSYLKPGDHLLMSDSVYEPARKLVGSFLTPYNIRCTFFAADGSD
VEEKIEAGTCMIYAECPGSLVYEMCDLPRLAEIARVRGLLLVADNTWGSGCQYRPLALGADVSIMAATKYLSGHSDVMMG
TVATTKDTWQALAERSDAFGMTVSPDDAYLVLRGTRSLAARLALHESHGLQVAYWLQSRSEVATVFCPALPDHPGHALWQ
RDCKGSNGLVSFELQPGFDQRAAERLIDSLQLFGIGASWGGYESLVTLADMRRARSVTDWSDRGQVIRLHIGLEDPADLL
RDLLQGFAALTA

Sequences:

>Translated_412_residues
MHQGHPGAPRMNKTSPPATSPSGASPLTRLVHAGKQPAFDGGRPVNPPVVRASTVLFDNTAQQREMRMRRGDERLFTYGA
RGNPTNFALEDVVSELEGAYRTRLFPTGLAAIAMTLLSYLKPGDHLLMSDSVYEPARKLVGSFLTPYNIRCTFFAADGSD
VEEKIEAGTCMIYAECPGSLVYEMCDLPRLAEIARVRGLLLVADNTWGSGCQYRPLALGADVSIMAATKYLSGHSDVMMG
TVATTKDTWQALAERSDAFGMTVSPDDAYLVLRGTRSLAARLALHESHGLQVAYWLQSRSEVATVFCPALPDHPGHALWQ
RDCKGSNGLVSFELQPGFDQRAAERLIDSLQLFGIGASWGGYESLVTLADMRRARSVTDWSDRGQVIRLHIGLEDPADLL
RDLLQGFAALTA
>Mature_412_residues
MHQGHPGAPRMNKTSPPATSPSGASPLTRLVHAGKQPAFDGGRPVNPPVVRASTVLFDNTAQQREMRMRRGDERLFTYGA
RGNPTNFALEDVVSELEGAYRTRLFPTGLAAIAMTLLSYLKPGDHLLMSDSVYEPARKLVGSFLTPYNIRCTFFAADGSD
VEEKIEAGTCMIYAECPGSLVYEMCDLPRLAEIARVRGLLLVADNTWGSGCQYRPLALGADVSIMAATKYLSGHSDVMMG
TVATTKDTWQALAERSDAFGMTVSPDDAYLVLRGTRSLAARLALHESHGLQVAYWLQSRSEVATVFCPALPDHPGHALWQ
RDCKGSNGLVSFELQPGFDQRAAERLIDSLQLFGIGASWGGYESLVTLADMRRARSVTDWSDRGQVIRLHIGLEDPADLL
RDLLQGFAALTA

Specific function: Also has cytotoxic activity toward osteogenic, osteosarcoma and tracheal cells, in vitro. The chemical basis for cell toxicity might be the formation and subsequent transfer of sulfane-sulfur to proteins, derived via beta-cystathionase cleavage of L-cysti

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=377, Percent_Identity=33.9522546419098, Blast_Score=188, Evalue=8e-48,
Organism=Homo sapiens, GI299473759, Length=375, Percent_Identity=32, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI25453487, Length=373, Percent_Identity=30.5630026809651, Blast_Score=155, Evalue=7e-38,
Organism=Escherichia coli, GI1789383, Length=395, Percent_Identity=39.2405063291139, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1790375, Length=374, Percent_Identity=32.0855614973262, Blast_Score=172, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17538055, Length=383, Percent_Identity=32.3759791122715, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17560056, Length=377, Percent_Identity=30.5039787798408, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=29.6495956873315, Blast_Score=167, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6321170, Length=337, Percent_Identity=36.7952522255193, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6319307, Length=355, Percent_Identity=29.5774647887324, Blast_Score=141, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6321254, Length=422, Percent_Identity=25.3554502369668, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=22.6544622425629, Blast_Score=96, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321904, Length=374, Percent_Identity=24.331550802139, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922554, Length=401, Percent_Identity=31.1720698254364, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006233
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 44878; Mature: 44878

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQGHPGAPRMNKTSPPATSPSGASPLTRLVHAGKQPAFDGGRPVNPPVVRASTVLFDNT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
AQQREMRMRRGDERLFTYGARGNPTNFALEDVVSELEGAYRTRLFPTGLAAIAMTLLSYL
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KPGDHLLMSDSVYEPARKLVGSFLTPYNIRCTFFAADGSDVEEKIEAGTCMIYAECPGSL
CCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHCCCEEEEEECCCCHH
VYEMCDLPRLAEIARVRGLLLVADNTWGSGCQYRPLALGADVSIMAATKYLSGHSDVMMG
HHHHHCCHHHHHHHHHCEEEEEEECCCCCCCCCCCEEECCCCEEEEHHHHHCCCCCEEEE
TVATTKDTWQALAERSDAFGMTVSPDDAYLVLRGTRSLAARLALHESHGLQVAYWLQSRS
EEECCHHHHHHHHHCCCCCCCEECCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCC
EVATVFCPALPDHPGHALWQRDCKGSNGLVSFELQPGFDQRAAERLIDSLQLFGIGASWG
CEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCCC
GYESLVTLADMRRARSVTDWSDRGQVIRLHIGLEDPADLLRDLLQGFAALTA
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHQGHPGAPRMNKTSPPATSPSGASPLTRLVHAGKQPAFDGGRPVNPPVVRASTVLFDNT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCC
AQQREMRMRRGDERLFTYGARGNPTNFALEDVVSELEGAYRTRLFPTGLAAIAMTLLSYL
HHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KPGDHLLMSDSVYEPARKLVGSFLTPYNIRCTFFAADGSDVEEKIEAGTCMIYAECPGSL
CCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHCCCEEEEEECCCCHH
VYEMCDLPRLAEIARVRGLLLVADNTWGSGCQYRPLALGADVSIMAATKYLSGHSDVMMG
HHHHHCCHHHHHHHHHCEEEEEEECCCCCCCCCCCEEECCCCEEEEHHHHHCCCCCEEEE
TVATTKDTWQALAERSDAFGMTVSPDDAYLVLRGTRSLAARLALHESHGLQVAYWLQSRS
EEECCHHHHHHHHHCCCCCCCEECCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCC
EVATVFCPALPDHPGHALWQRDCKGSNGLVSFELQPGFDQRAAERLIDSLQLFGIGASWG
CEEEEEECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHEEECCCCC
GYESLVTLADMRRARSVTDWSDRGQVIRLHIGLEDPADLLRDLLQGFAALTA
CHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8463265 [H]